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Intermediate enzyme kinetics
166
TIBS - July 1976
In general, this book provides a solid substrate complex interacts with the enzyme are heavily stressed. The added com- grounding in the practical and theoretical plexities that occur when a metal activator aspects of enzyme kinetics. The author is plays a dual role in binding to the enzyme careful to point out the limitations of the and the substrate are, however, ignored. various methods discussed and the added This book differs from most works on complexities that can occur if isomerizathis subject in that a considerable amount tions or abortive complex formation takes of space is devoted to practical details. In place. Anyone wishing to learn about the these sections, on the design and conduct subject should benefit from reading this of kinetic experiments, the author provides book, but I feel that the benefit might have a great deal of sound advice which could been greater if the author had developed be read with advantage by anyone starting the principles instead of concentrating on kinetic work. The author's interest in pure the particular. KEITH TIPTON kinetics is, however, evident, since his discussions on the choice of assay conditions Keith Tipton is Lecturer in Biochemistry at the Univercontain no suggestion that there might be sity of Cambridge. Cambridge. U.K. some advantage in trying to approximate these to the conditions that might be expected within the cell. The discussion of the theory of kinetic Alcohol a n d A b n o r m a l Protein Bioanalysis is extremely comprehensive. All synthesis. Biochemical a n d Clinical likely two- and three-substrate reaction edited by M.A. Rothschild, M. Oratz and S.S. Schreiber, published by Pergamon mechanisms are considered and the complete kinetic equations are listed for many Press, New York. $25.-/$10.50 (hard case) (xvff+ 519 pages) of them in an appendix. The types of inhibition given by substrates, products, alternative substrates and alternative products This book is a collection of 24 separate are tabulated for all the mechanisms as essays, each from a different authorship, are the Haldane and Dalziel relationships. bearing on problems relating to the effects In concentrating on this array of particular of alcohol and/or nutritional factors (espemechanisms many of the general principles cially malnutrition) on protein metabolism underlying the analyses are too easily lost. and protein synthesis. The title does not In the section on dead-end inhibitors the do full justice to the contents. About half author lists Cleland's rules and briefly sug- the papers deal with general aspects of proT . H . PENNINGTON gests that they may be understood from tein metabolism, such as methods of investigation, the biochemistry and pathology T.H. Pennington is Senior Lecturer at the b~stitute a consideration of the various pathways of Virology, University of Glasgow, Glasgow, U.K. in which each of the intermediates in the of tissue proteins, and protein transport reaction mechanism may be formed. This through membranes. The quality of the articles, as is not unimportant concept is not developed further, however, and indeed it is not men- usual in multi-author volumes, is uneven. tioned in the discussion of product inhibi- Some of the essays are like chapters of advanced text-books, merely repeating intion. Initial Rate Enzyme Kinetics Cleland's formulation is used for repre- formation already published in previous by H.J. Fromm, published by Sprhzger senting kinetic reaction pathways except reviews, while other authors approach Verlag, Berlin-Heidelberg-New York. in the many instances when this proves their task in a more original manner and to be inadequate, leading to a rather con- offer new and interesting vistas. The reDM78.- ($32.-) (321 pages) fusing mixture of presentations. Occa- viewer found the chapters on the regulaThe presentation of enzyme kinetic theory sionally symbols are not defined when they tion of protein synthesis in relation to in an easily digestible form has always been are first used and there is no general table amino acid supply (H.N. Munro); on a difficult problem, and the solutions that of symbols. The printing is sometimes un- metabolic adaptation to protein deficiency have been adopted by different writers clear and there are a few confusing printing (J. C. Waterlow); on effects of ethanol on range from the strictly mathematical errors. In his choice of illustrative exam- intestinal amino acid transport (Tsun approach to the formulation of qualitative ples the author relies heavily on his own Chang); on diet and protein metabolism 'rules' to enable the kinetic mechanism published work particularly with hexok- in skeletal muscle (D.J. Millward); and obeyed by an enzyme to be deduced with inase. For example, the behaviour of hex- on the control of protein turnover in the the minimum of thought. This book, okinase is used to explain the artefacts that isolated perfused rat heart, of special which is based on a graduate course taught may arise when a metal-substrate complex value. Morphological sections deal with by the author, takes a path between these interacts with the enzyme, in preference changes produced by ethanol, in cardiac two extremes. The material covered is res- to any of the earlier work on such systems. muscle and liver. Rosenoer discusses the tricted to initial-rate and isotope-exchange The book contains details of two useful nutritional potential of enterohepatically kinetics, there being no general treatment computer programs as an appendix, one recycled urea nitrogen in man. Other of the kinetics of transient states. The for deriving steady-state equations a n d chapters deal with alcoholic diseases of the choice of topics is occasionally somewhat one for the statistical treatment of kinetic cardiovascular system, liver and brain. arbitrary, for example the kinetics of acti- data. It is a pity, however, that the latter There is a chapter by H.O. Conn on the vators are not considered in any detail but program is in a language that is not at therapy of alcoholic liver disease. the problems that can arise when a metal- present widely available. Although quite a few of the topics have
of Chapter IIl that only oncorna-, rea-, and poxviruses possess virion-associated transcriptases. I disagree with the interpretation of the antipoxvirus effects of rifampicin in Chapter VII; most workers are agreed that the macrocyclic ring is necessary for activity and that the active site does not reside in the hydrazone side chain. In addition, Becker states that the inhibition of virus envelope formation follows the inhibition of cleavage of a structural polypeptide precursor, whereas the experimental evidence in fact suggests the reverse. The statement in Table III that rifampicin inhibits poxvirus coat protein synthesis has possibly crept in as an oversimplification, synthesis being substituted unintentionally for processing. With the exception of these criticisms and one or two other over-simplifications and omissions, the monograph presents a concise and accurate account of inhibitors of animal virus growth, and will serve as a useful review of this subject. The lengthy interval between the completion of the literature survey and the publication date means that the book is already somewhat out of date. As is usual with Karger, the book is well produced; an innovation for the 'Monographs in Virology' series is the soft cover. Unfortunately this has not had any inhibitory effect on the now-to-beexpected high price.
Intermediate enzyme kinetics
Dated but useful essays
TIBS - July 1976
In general, this book provides a solid substrate complex interacts with the enzyme are heavily stressed. The added com- grounding in the practical and theoretical plexities that occur when a metal activator aspects of enzyme kinetics. The author is plays a dual role in binding to the enzyme careful to point out the limitations of the and the substrate are, however, ignored. various methods discussed and the added This book differs from most works on complexities that can occur if isomerizathis subject in that a considerable amount tions or abortive complex formation takes of space is devoted to practical details. In place. Anyone wishing to learn about the these sections, on the design and conduct subject should benefit from reading this of kinetic experiments, the author provides book, but I feel that the benefit might have a great deal of sound advice which could been greater if the author had developed be read with advantage by anyone starting the principles instead of concentrating on kinetic work. The author's interest in pure the particular. KEITH TIPTON kinetics is, however, evident, since his discussions on the choice of assay conditions Keith Tipton is Lecturer in Biochemistry at the Univercontain no suggestion that there might be sity of Cambridge. Cambridge. U.K. some advantage in trying to approximate these to the conditions that might be expected within the cell. The discussion of the theory of kinetic Alcohol a n d A b n o r m a l Protein Bioanalysis is extremely comprehensive. All synthesis. Biochemical a n d Clinical likely two- and three-substrate reaction edited by M.A. Rothschild, M. Oratz and S.S. Schreiber, published by Pergamon mechanisms are considered and the complete kinetic equations are listed for many Press, New York. $25.-/$10.50 (hard case) (xvff+ 519 pages) of them in an appendix. The types of inhibition given by substrates, products, alternative substrates and alternative products This book is a collection of 24 separate are tabulated for all the mechanisms as essays, each from a different authorship, are the Haldane and Dalziel relationships. bearing on problems relating to the effects In concentrating on this array of particular of alcohol and/or nutritional factors (espemechanisms many of the general principles cially malnutrition) on protein metabolism underlying the analyses are too easily lost. and protein synthesis. The title does not In the section on dead-end inhibitors the do full justice to the contents. About half author lists Cleland's rules and briefly sug- the papers deal with general aspects of proT . H . PENNINGTON gests that they may be understood from tein metabolism, such as methods of investigation, the biochemistry and pathology T.H. Pennington is Senior Lecturer at the b~stitute a consideration of the various pathways of Virology, University of Glasgow, Glasgow, U.K. in which each of the intermediates in the of tissue proteins, and protein transport reaction mechanism may be formed. This through membranes. The quality of the articles, as is not unimportant concept is not developed further, however, and indeed it is not men- usual in multi-author volumes, is uneven. tioned in the discussion of product inhibi- Some of the essays are like chapters of advanced text-books, merely repeating intion. Initial Rate Enzyme Kinetics Cleland's formulation is used for repre- formation already published in previous by H.J. Fromm, published by Sprhzger senting kinetic reaction pathways except reviews, while other authors approach Verlag, Berlin-Heidelberg-New York. in the many instances when this proves their task in a more original manner and to be inadequate, leading to a rather con- offer new and interesting vistas. The reDM78.- ($32.-) (321 pages) fusing mixture of presentations. Occa- viewer found the chapters on the regulaThe presentation of enzyme kinetic theory sionally symbols are not defined when they tion of protein synthesis in relation to in an easily digestible form has always been are first used and there is no general table amino acid supply (H.N. Munro); on a difficult problem, and the solutions that of symbols. The printing is sometimes un- metabolic adaptation to protein deficiency have been adopted by different writers clear and there are a few confusing printing (J. C. Waterlow); on effects of ethanol on range from the strictly mathematical errors. In his choice of illustrative exam- intestinal amino acid transport (Tsun approach to the formulation of qualitative ples the author relies heavily on his own Chang); on diet and protein metabolism 'rules' to enable the kinetic mechanism published work particularly with hexok- in skeletal muscle (D.J. Millward); and obeyed by an enzyme to be deduced with inase. For example, the behaviour of hex- on the control of protein turnover in the the minimum of thought. This book, okinase is used to explain the artefacts that isolated perfused rat heart, of special which is based on a graduate course taught may arise when a metal-substrate complex value. Morphological sections deal with by the author, takes a path between these interacts with the enzyme, in preference changes produced by ethanol, in cardiac two extremes. The material covered is res- to any of the earlier work on such systems. muscle and liver. Rosenoer discusses the tricted to initial-rate and isotope-exchange The book contains details of two useful nutritional potential of enterohepatically kinetics, there being no general treatment computer programs as an appendix, one recycled urea nitrogen in man. Other of the kinetics of transient states. The for deriving steady-state equations a n d chapters deal with alcoholic diseases of the choice of topics is occasionally somewhat one for the statistical treatment of kinetic cardiovascular system, liver and brain. arbitrary, for example the kinetics of acti- data. It is a pity, however, that the latter There is a chapter by H.O. Conn on the vators are not considered in any detail but program is in a language that is not at therapy of alcoholic liver disease. the problems that can arise when a metal- present widely available. Although quite a few of the topics have
of Chapter IIl that only oncorna-, rea-, and poxviruses possess virion-associated transcriptases. I disagree with the interpretation of the antipoxvirus effects of rifampicin in Chapter VII; most workers are agreed that the macrocyclic ring is necessary for activity and that the active site does not reside in the hydrazone side chain. In addition, Becker states that the inhibition of virus envelope formation follows the inhibition of cleavage of a structural polypeptide precursor, whereas the experimental evidence in fact suggests the reverse. The statement in Table III that rifampicin inhibits poxvirus coat protein synthesis has possibly crept in as an oversimplification, synthesis being substituted unintentionally for processing. With the exception of these criticisms and one or two other over-simplifications and omissions, the monograph presents a concise and accurate account of inhibitors of animal virus growth, and will serve as a useful review of this subject. The lengthy interval between the completion of the literature survey and the publication date means that the book is already somewhat out of date. As is usual with Karger, the book is well produced; an innovation for the 'Monographs in Virology' series is the soft cover. Unfortunately this has not had any inhibitory effect on the now-to-beexpected high price.
Intermediate enzyme kinetics
Dated but useful essays