- Email: [email protected]
Interplay between p63 and miR-138 in tongue carcinogenesis
Oral Presentation Outcomes were bone regeneration measured on conebeam computed tomography (conebeam-CT) X-rays, bone histology and patient satisfaction. Findings: Postoperative conebeam-CTs showed new bone formation in the non-augmented side, however there was less opacity seen than in the conventionally augmented side. Six months after sinus membrane elevation enough bone was formed in all cases for implant placement. The bone biopsies showed newly formed bone on the experimental side (osteoinductivity). On the conventional side, a typical mixture of autogenous and xenogenous bone was seen (osteoconductivity). Conclusions: The results suggest that the elevation of the sinus membrane will lead to new bone formation. However, the osteogenetic capacity of the sinus membrane is not proven, as this membrane cannot be regarded as periosteal tissue. http://dx.doi.org/10.1016/j.ijom.2015.08.672 Compression fracture of posterior lateral maxillary sinus wall secondary to isolated mandibular fractures – a domino effect H.Y. Lin ∗ , C.M. Chang, P.Y. Chang, K.J. Chen, Y.F. Wu, M.Yc. Chen Department of OMS, Taichung China Medical University Hospital, Taiwan Background: The purpose of this study is to describe an incidental findings of posterior lateral sinus wall fracture occurred in primarily isolated mandibular fractures and try to propose a hypothesis of its mechanism. Objectives and methods: By reviewing facial bone CT images of 358 individual’s sustained facial injury and mandibular fractures from January 2010 through August 2014, we have identified 245 patients enrolled in this retrospective study according to the following exclusion criteria: (1) concomitant mid-face fractures; (2) poor image quality of facial bone CT; (3) fractures limited to dentoalveolar portion of mandible. Findings: The incidence of maxillary sinus posterior lateral wall fracture in isolated mandible fracture population was 18%, with green stick fractures (n = 7) and compression fractures (n = 37), found in 44 individuals. Analysis of the 83 mandibular fractures sites of these 44 cases revealed that the most commonly affected area was located at contralateral parasymphysis (22%, n = 18) followed by the ipsilateral mandibular angle (16%, n = 13). Concurrent maxillary sinus posterior lateral wall fracture with two site mandible fractures was most often (55%) and followed by only one site mandible fracture (30%). Conclusions: We postulated fractures of posterior lateral sinus walls as a “domino effect” of direct pounding by the severely displaced ipsilateral coronoid process and transient dislocation of condylar head when primary mandibular fractures occurred.
e103
Dynamic cellular and molecular modulations of diabetes mediated head and neck carcinogenesis C.J. Liu 1,2,4,∗ , W.J. Chang 4,5 , C.Y. Chen 4 , F.J. Sun 2,3 , H.W. Cheng 2 , T.Y. Chen 4 , S.C. Lin 4,5 , W.C. Li 4 1
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan 2 Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan 3 Mackay College of Medicine, Nursing and Management, Taipei, Taiwan 4 Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan 5 Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan
Background: Head and neck squamous cell carcinoma (HNSCC) is one of the most prevalent neoplasms worldwide. While numerous potent dietary insults were considered as oncogenic players for HNSCC development, the impact of metabolic imbalance was less emphasized during HNSCC carcinogenesis. Previous preclinical and epidemiological investigations showed that DM could possibly be correlated with greater incidence and poorer prognosis in HNSCC patients; however, the outcomes from different groups are contradictive and underlying mechanisms remains elusive. Objectives and methods: In the present study, the changes of cellular malignancy in response to prolonged glucose incubation in HNSCC cells were examined. We retrospectively reviewed 613 patients with HNSCC. DM and other potential prognostic variables were chosen for analysis in this study. Univariate and multivariate analyses were conducted to identify prognostic factors associated with survival. Findings: The results demonstrated that hyperglycemia enhanced HNSCC cell malignancy over time through suppression of cell differentiation, promotion of cell motility, increased resistance to cisplatin, and up-regulation of the nutrient-sensing Akt/AMPK-mTORC1 pathway. Whereas DM pathology led to a higher percentage of neck lymph nodal metastasis and poorer prognosis in HNSCC patients. Conclusion: The present study confirms that hyperglycemia and DM could enhance HNSCC malignancy and the outcomes are of great benefit in providing better anti-cancer treatment strategy for DM patients with HNSCC. Regional session: Asia. http://dx.doi.org/10.1016/j.ijom.2015.08.674 Interplay between p63 and miR-138 in tongue carcinogenesis Z. Zhuang 1 , N. Xie 1,3 , C. Wang 1 , J. Hu 1 , J. Hou 1 , T. Wang 2 , H. Huang 1 , X. Liu 1,∗ 1
http://dx.doi.org/10.1016/j.ijom.2015.08.673
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China 2 The Affiliated Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China 3 Center for Immunology and Infectious Diseases, SRI International, VA, United States Background and objectives: The role of p63 and miRNA in tumorigenesis remains largely unknown. This study was aim to determine the expression and functional regulation of p63 and miR-138 in tongue cancer (TC), and further to analyze their correlation both in vitro and in vivo.
e104
Oral Presentation
Methods: Levels of p63 and miR-138 in TC (n = 103) were investigated in vivo and in vitro. Their correlations with the clinicopathological parameters and prognosis were analyzed. The interactive regulation of miR-138 and p63 was further evaluated. Findings: Increased p63 and reduced miR-138 expression was observed in tongue cancers (p < 0.05). Notably, a more elevated expression for p63 appeared in the invasive tumor front compared with superficial or central counterparts (p < 0.05). A negative correlation between p63 and miR-138 expression can be observed. The levels of p63 and miR-138 are associated with cell differentiation, cervical lymph node metastasis status and clinical stage as well. Overexpression of p63 was correlated with reduced overall survival, and can be served as an independent prognostic factor. More, a negative correlation between p63 (Np63 predominant) and miR-138 expression can be observed in vitro (p < 0.05). Ectopic expression of miR-138 led to reduced p63 level and suppressed cell invasion and migration. Conversely, knockdown of p63 enhanced miR-138 expression and reversed the aggressive phenotype by regulating cell differentiation. Conclusions: The deregulation of p63 and miR-138 are common molecular events in tongue cancer progression. Our findings suggest that p63 and miR-138 may collaboratively play a role in tongue carcinogenesis. http://dx.doi.org/10.1016/j.ijom.2015.08.675 Myoepithelioma of hard palate – report of two cases and literature review C.M. Lo ∗ , K.H. Yeung Oral Maxillofacial Surgery and Dental Unit, Prince of Wales Hospital, Hong Kong Introduction: Myoepithelioma was defined as a benign salivary gland tumor composed almost exclusively only myoepithelial cells without ductal component. It was well known for its rarity, accounting for about 1% of the salivary gland tumor. The objective of this paper was to present two cases with literature review and determines the clinical, radiographical and histological features of this rare pathology. Case reports: Two patients (a 61 years old male and a 51 years old female) presented with myoepithelioma on hard palate were treated in the Oral Maxillofacial Surgery and Dental Unit, Prince of Wales Hospital, Hong Kong. Both cases were presented with smooth, non-ulcerated palatal swelling. The histological diagnosis was confirmed by incisional biopsy. Both were treated with surgical excision and further diagnosed plasmacystoid histological subtype. No recurrence was seen in both cases after 3 years. Conclusion: Combining the current two case reports with the other eight cases of palatal myoepithelioma previously reported in the literature indicated that myoepithelioma is a rare tumor, but should also be included in the differential diagnosis of palatal swelling. It can be adequately treated by surgical excision with margin of healthy borders. http://dx.doi.org/10.1016/j.ijom.2015.08.676
Practical classification system for horizontal/mesio-angular impaction of mandibular third molars W.F. Lo ∗ , K.J. Chen, M.Yc. Chen China Medical University Hospital, Taichung, Taiwan Background: As we know, Pell–Gregory’s classification provide the benchmark of grading the difficulty of vertically impacted mandibular third molars, which is based on the amount of tooth covered by anterior border of the ascending ramus and the depth of the impaction relative to the adjacent tooth, 2nd molars. However, Pell–Gregory’s system cannot be applied to evaluate horizontal/mesio-angular impactions which are much more commonly encountered clinical scenario. Objectives: The purpose of this study was to provide a clear and efficient classification system for both horizontal and mesioangular impaction of mandibular third molars. Methods: There are 412 patients, aged from 17 to 61 years old, who had odontectomy of impacted mandibular third molars by 2 designated surgeons, one attending and one resident of OMS department in the year of 2014. We recorded the operation time, pain evaluation by visual analog scale (VAS) at postoperative day-7 of these procedures (n = 433). Preoperative panoramic radiographs were routinely obtained for grading of all impacted mandibular 3rd molars according to the Taichung China Medical University (TCMU) Classification system by another senior oral maxillofacial surgeon. Findings: After linear regression analysis, there is significant (p-value <0.05) correlation of operation time and the TCMU Classification system. However, there is no significant relationship between pain scale (VAS) and the grading system. Conclusions: Odontectomy of impacted third molars is one of the most common practice for oral maxillofacial surgeons. It is very important for surgeons to estimate or expect the difficulties beforehand. TCMU Classification offers a simple and clear grading system for peri-operative evaluation of horizontal and mesio-angular impaction of mandibular 3rd molars. http://dx.doi.org/10.1016/j.ijom.2015.08.677 Creating a programmed, self-destructing protein drug, Kazecin, that causes the apoptotic progression of the oral SAS-tumor W.L. Lo 1,2,∗ , D.T. Chen 3,4 , K.W. Chang 1,2 , S.Y. Kao 1,2 , C.S. Chang 1,2 , A. Lin 1 1
School of Dentistry, National Yang-Ming University, Taipei, Taiwan 2 Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan 3 Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan 4 VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan Background: Based on the ribosome-inactivating nature of ribotoxin.1,2 Objectives: The concept of ‘Kamikaze ribotoxin’ is formulated to create a potential protein drug targeted against tumor growth by protein engineering. Methods: The engineered Kamikaze ribotoxin (Kazecin) equips a structure entity of alpha-sarcin with a cell penetrating peptide and a caspase-3-sensitive peptide.
e103
Dynamic cellular and molecular modulations of diabetes mediated head and neck carcinogenesis C.J. Liu 1,2,4,∗ , W.J. Chang 4,5 , C.Y. Chen 4 , F.J. Sun 2,3 , H.W. Cheng 2 , T.Y. Chen 4 , S.C. Lin 4,5 , W.C. Li 4 1
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan 2 Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan 3 Mackay College of Medicine, Nursing and Management, Taipei, Taiwan 4 Institute of Oral Biology and Department of Dentistry, School of Dentistry, National Yang-Ming University, Taipei, Taiwan 5 Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan
Background: Head and neck squamous cell carcinoma (HNSCC) is one of the most prevalent neoplasms worldwide. While numerous potent dietary insults were considered as oncogenic players for HNSCC development, the impact of metabolic imbalance was less emphasized during HNSCC carcinogenesis. Previous preclinical and epidemiological investigations showed that DM could possibly be correlated with greater incidence and poorer prognosis in HNSCC patients; however, the outcomes from different groups are contradictive and underlying mechanisms remains elusive. Objectives and methods: In the present study, the changes of cellular malignancy in response to prolonged glucose incubation in HNSCC cells were examined. We retrospectively reviewed 613 patients with HNSCC. DM and other potential prognostic variables were chosen for analysis in this study. Univariate and multivariate analyses were conducted to identify prognostic factors associated with survival. Findings: The results demonstrated that hyperglycemia enhanced HNSCC cell malignancy over time through suppression of cell differentiation, promotion of cell motility, increased resistance to cisplatin, and up-regulation of the nutrient-sensing Akt/AMPK-mTORC1 pathway. Whereas DM pathology led to a higher percentage of neck lymph nodal metastasis and poorer prognosis in HNSCC patients. Conclusion: The present study confirms that hyperglycemia and DM could enhance HNSCC malignancy and the outcomes are of great benefit in providing better anti-cancer treatment strategy for DM patients with HNSCC. Regional session: Asia. http://dx.doi.org/10.1016/j.ijom.2015.08.674 Interplay between p63 and miR-138 in tongue carcinogenesis Z. Zhuang 1 , N. Xie 1,3 , C. Wang 1 , J. Hu 1 , J. Hou 1 , T. Wang 2 , H. Huang 1 , X. Liu 1,∗ 1
http://dx.doi.org/10.1016/j.ijom.2015.08.673
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, China 2 The Affiliated Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China 3 Center for Immunology and Infectious Diseases, SRI International, VA, United States Background and objectives: The role of p63 and miRNA in tumorigenesis remains largely unknown. This study was aim to determine the expression and functional regulation of p63 and miR-138 in tongue cancer (TC), and further to analyze their correlation both in vitro and in vivo.
e104
Oral Presentation
Methods: Levels of p63 and miR-138 in TC (n = 103) were investigated in vivo and in vitro. Their correlations with the clinicopathological parameters and prognosis were analyzed. The interactive regulation of miR-138 and p63 was further evaluated. Findings: Increased p63 and reduced miR-138 expression was observed in tongue cancers (p < 0.05). Notably, a more elevated expression for p63 appeared in the invasive tumor front compared with superficial or central counterparts (p < 0.05). A negative correlation between p63 and miR-138 expression can be observed. The levels of p63 and miR-138 are associated with cell differentiation, cervical lymph node metastasis status and clinical stage as well. Overexpression of p63 was correlated with reduced overall survival, and can be served as an independent prognostic factor. More, a negative correlation between p63 (Np63 predominant) and miR-138 expression can be observed in vitro (p < 0.05). Ectopic expression of miR-138 led to reduced p63 level and suppressed cell invasion and migration. Conversely, knockdown of p63 enhanced miR-138 expression and reversed the aggressive phenotype by regulating cell differentiation. Conclusions: The deregulation of p63 and miR-138 are common molecular events in tongue cancer progression. Our findings suggest that p63 and miR-138 may collaboratively play a role in tongue carcinogenesis. http://dx.doi.org/10.1016/j.ijom.2015.08.675 Myoepithelioma of hard palate – report of two cases and literature review C.M. Lo ∗ , K.H. Yeung Oral Maxillofacial Surgery and Dental Unit, Prince of Wales Hospital, Hong Kong Introduction: Myoepithelioma was defined as a benign salivary gland tumor composed almost exclusively only myoepithelial cells without ductal component. It was well known for its rarity, accounting for about 1% of the salivary gland tumor. The objective of this paper was to present two cases with literature review and determines the clinical, radiographical and histological features of this rare pathology. Case reports: Two patients (a 61 years old male and a 51 years old female) presented with myoepithelioma on hard palate were treated in the Oral Maxillofacial Surgery and Dental Unit, Prince of Wales Hospital, Hong Kong. Both cases were presented with smooth, non-ulcerated palatal swelling. The histological diagnosis was confirmed by incisional biopsy. Both were treated with surgical excision and further diagnosed plasmacystoid histological subtype. No recurrence was seen in both cases after 3 years. Conclusion: Combining the current two case reports with the other eight cases of palatal myoepithelioma previously reported in the literature indicated that myoepithelioma is a rare tumor, but should also be included in the differential diagnosis of palatal swelling. It can be adequately treated by surgical excision with margin of healthy borders. http://dx.doi.org/10.1016/j.ijom.2015.08.676
Practical classification system for horizontal/mesio-angular impaction of mandibular third molars W.F. Lo ∗ , K.J. Chen, M.Yc. Chen China Medical University Hospital, Taichung, Taiwan Background: As we know, Pell–Gregory’s classification provide the benchmark of grading the difficulty of vertically impacted mandibular third molars, which is based on the amount of tooth covered by anterior border of the ascending ramus and the depth of the impaction relative to the adjacent tooth, 2nd molars. However, Pell–Gregory’s system cannot be applied to evaluate horizontal/mesio-angular impactions which are much more commonly encountered clinical scenario. Objectives: The purpose of this study was to provide a clear and efficient classification system for both horizontal and mesioangular impaction of mandibular third molars. Methods: There are 412 patients, aged from 17 to 61 years old, who had odontectomy of impacted mandibular third molars by 2 designated surgeons, one attending and one resident of OMS department in the year of 2014. We recorded the operation time, pain evaluation by visual analog scale (VAS) at postoperative day-7 of these procedures (n = 433). Preoperative panoramic radiographs were routinely obtained for grading of all impacted mandibular 3rd molars according to the Taichung China Medical University (TCMU) Classification system by another senior oral maxillofacial surgeon. Findings: After linear regression analysis, there is significant (p-value <0.05) correlation of operation time and the TCMU Classification system. However, there is no significant relationship between pain scale (VAS) and the grading system. Conclusions: Odontectomy of impacted third molars is one of the most common practice for oral maxillofacial surgeons. It is very important for surgeons to estimate or expect the difficulties beforehand. TCMU Classification offers a simple and clear grading system for peri-operative evaluation of horizontal and mesio-angular impaction of mandibular 3rd molars. http://dx.doi.org/10.1016/j.ijom.2015.08.677 Creating a programmed, self-destructing protein drug, Kazecin, that causes the apoptotic progression of the oral SAS-tumor W.L. Lo 1,2,∗ , D.T. Chen 3,4 , K.W. Chang 1,2 , S.Y. Kao 1,2 , C.S. Chang 1,2 , A. Lin 1 1
School of Dentistry, National Yang-Ming University, Taipei, Taiwan 2 Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan 3 Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan 4 VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan Background: Based on the ribosome-inactivating nature of ribotoxin.1,2 Objectives: The concept of ‘Kamikaze ribotoxin’ is formulated to create a potential protein drug targeted against tumor growth by protein engineering. Methods: The engineered Kamikaze ribotoxin (Kazecin) equips a structure entity of alpha-sarcin with a cell penetrating peptide and a caspase-3-sensitive peptide.