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Interrelationships of functional, biochemical and morphological variables in catecholamine cardiotoxicity
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ABSTRACTS / Journal of Molecular and Cellular Cardiology 42 (2007) S238–S246
characteristics that determine the selection of revascularization method. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.731
TIMI risk score in patients with unstable angina/non-st elevation mi who underwent CABG revascularization G. Pampu, I. Craciunescu, M. Serban, S. Vasile, H. Moldovan, D. Deleanu, C. Ginghina. Institute of Cardiovascular Diseases, Bucharest, Romania Background: TIMI risk score has not been yet tested in special categories as patients revascularized by CABG. Objective: The purpose of the study was to investigate the relationships between the TIMI risk score and clinical outcome in patients with UA/NSTEMI and CABG revascularization. Methods: 158 patients with UA/NSTEMI who underwent myocardial revascularization by CABG were enrolled and followed-up over a 2 years period. We registered all-cause mortality, the major cardiac adverse events (MACE), the patency of coronary grafts and the necessity of repeated revascularization. Results: Patients were divided into 4 groups, according to the number of MACE and consecutive hospital admissions after the CABG. Mean values of TIMI risk score were 3.16 in group a with 0/1 MACE, 4.75 in group B with 2/3 MACE, 5.23 in group C with 4/5 MACE and 6.27 in group D with ≥ 6 MACE (P < 0.001 by ANOVA analyse). All patients underwent coronarographic control after CABG. Highest TIMI risk score were registered in patients with all grafts occluded (mean value of 6.33 ± 1.26). Cut off value of TIMI risk score for patency of venous grafts was 3.35, with a specificity of 86.6% and a sensitivity of 82.1%. Patients with repeated revascularization within 2 years after the first CABG (either repeated CABG or PTCA) had a higher TIMI risk score (7.12 ± 2.87 versus 3.06 ± 1.23, p < 0.001). Conclusion: TIMI risk score is a valuable predictor of clinical outcome and patency of grafts in patients with UA/NSTEMI who underwent CABG revascularization. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.732
Interrelationships of functional, biochemical and morphological variables in catecholamine cardiotoxicity Přemysl Mladěnka, Radek Hrdina, Zuzka Bobrovová, Mojmír Hübl, Petr Nachtigal, Jan Škrle, Vladimir Semecký. Charles Univ., Fac Pharmacy, Czech Republic Coronary heart disease and mainly its most serious form– acute myocardial infarction (AIM)–represent in spite of large
cardiovascular research still the most common cause of death in the developed countries. Isoprenaline (ISO), a synthetic catecholamine, has the ability to evoke myocardial impairment in some aspects very similar to AIM in human. This study was designated to verify these injuries from biochemical, functional and histological point of views and to establish correlations among different parameters. 18 male Wistar rats were divided into two groups—control and ISO. After 24 h of ISO (100 mg/ kg s.c.) or saline administration, measurements were performed. Very significant differences were shown in various parameters between both groups. Shortly, ISO brought about significant impairment of myocardial function, marked elevation of cardiac troponin T in plasma, calcium overload in the myocardium and was associated with myocardial necrosis. Some significant correlations were revealed, in particular, of cardiac troponin T with calcium overload and functional variables. In conclusion. ISO-myocardial injury may be a suitable model of AIM, in addition, cardiac troponin T could precisely indicate the extent of functional damages of the myocardium. Acknowledgments This work was supported by grants 98/2005/C/FaF and 94/ 2006/C/FaF of Charles University in Prague (Czech Republic). Keywords: Infarction; Cardiomyopathies; Calcium doi:10.1016/j.yjmcc.2007.03.733
Myocardial ischemia prevention in swine with autologous ozoneized blood transfusion Andrea Barone, Javier Woskoboinic, Aníbal Grangeat, Ricardo Pérez, Ignacio Berra, Guillermo Berra, José Milei. ININCA, Univ Buenos Aires, Argentina Although ozone therapy has been widely used, no attempts have been made to evaluate its protective effects in acute myocardial ischemia. Because of this, a pilot study with a few number of animals was initiated. Under surgical conditions, 200 ml of autologous venous blood, enriched with 20 ml ozone (major autohemotransfusion—MAHT) were infused through the internal jugular vein of adult swines (n = 3), 10 min before the proximal right coronary artery was occluded with a balloon for angioplasty. All animals were sacrificed immediately after having completed the sequence: ST segment elevation, ventricular fibrillation and cardiac arrest. No evident necrotic/ ischemic areas as assessed by tripheniltetrazolium chloride (TTC) were detected and histological sections revealed no damage in myocardial tissue. Conversely, swine infused with plain autologous venous blood (n = 3) and submitted to the same protocol showed preservation in only 81.4 ± 11.6% of the total myocardium, with infarcted/ischemic areas of 14 ± 8.5% and 4.5 ± 3.1%, respectively, (TTC stain), while diffuse monofocal–multifocal necrotic areas were found in microscopical
ABSTRACTS / Journal of Molecular and Cellular Cardiology 42 (2007) S238–S246
characteristics that determine the selection of revascularization method. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.731
TIMI risk score in patients with unstable angina/non-st elevation mi who underwent CABG revascularization G. Pampu, I. Craciunescu, M. Serban, S. Vasile, H. Moldovan, D. Deleanu, C. Ginghina. Institute of Cardiovascular Diseases, Bucharest, Romania Background: TIMI risk score has not been yet tested in special categories as patients revascularized by CABG. Objective: The purpose of the study was to investigate the relationships between the TIMI risk score and clinical outcome in patients with UA/NSTEMI and CABG revascularization. Methods: 158 patients with UA/NSTEMI who underwent myocardial revascularization by CABG were enrolled and followed-up over a 2 years period. We registered all-cause mortality, the major cardiac adverse events (MACE), the patency of coronary grafts and the necessity of repeated revascularization. Results: Patients were divided into 4 groups, according to the number of MACE and consecutive hospital admissions after the CABG. Mean values of TIMI risk score were 3.16 in group a with 0/1 MACE, 4.75 in group B with 2/3 MACE, 5.23 in group C with 4/5 MACE and 6.27 in group D with ≥ 6 MACE (P < 0.001 by ANOVA analyse). All patients underwent coronarographic control after CABG. Highest TIMI risk score were registered in patients with all grafts occluded (mean value of 6.33 ± 1.26). Cut off value of TIMI risk score for patency of venous grafts was 3.35, with a specificity of 86.6% and a sensitivity of 82.1%. Patients with repeated revascularization within 2 years after the first CABG (either repeated CABG or PTCA) had a higher TIMI risk score (7.12 ± 2.87 versus 3.06 ± 1.23, p < 0.001). Conclusion: TIMI risk score is a valuable predictor of clinical outcome and patency of grafts in patients with UA/NSTEMI who underwent CABG revascularization. Keywords: Ventricular function; Ischemia doi:10.1016/j.yjmcc.2007.03.732
Interrelationships of functional, biochemical and morphological variables in catecholamine cardiotoxicity Přemysl Mladěnka, Radek Hrdina, Zuzka Bobrovová, Mojmír Hübl, Petr Nachtigal, Jan Škrle, Vladimir Semecký. Charles Univ., Fac Pharmacy, Czech Republic Coronary heart disease and mainly its most serious form– acute myocardial infarction (AIM)–represent in spite of large
cardiovascular research still the most common cause of death in the developed countries. Isoprenaline (ISO), a synthetic catecholamine, has the ability to evoke myocardial impairment in some aspects very similar to AIM in human. This study was designated to verify these injuries from biochemical, functional and histological point of views and to establish correlations among different parameters. 18 male Wistar rats were divided into two groups—control and ISO. After 24 h of ISO (100 mg/ kg s.c.) or saline administration, measurements were performed. Very significant differences were shown in various parameters between both groups. Shortly, ISO brought about significant impairment of myocardial function, marked elevation of cardiac troponin T in plasma, calcium overload in the myocardium and was associated with myocardial necrosis. Some significant correlations were revealed, in particular, of cardiac troponin T with calcium overload and functional variables. In conclusion. ISO-myocardial injury may be a suitable model of AIM, in addition, cardiac troponin T could precisely indicate the extent of functional damages of the myocardium. Acknowledgments This work was supported by grants 98/2005/C/FaF and 94/ 2006/C/FaF of Charles University in Prague (Czech Republic). Keywords: Infarction; Cardiomyopathies; Calcium doi:10.1016/j.yjmcc.2007.03.733
Myocardial ischemia prevention in swine with autologous ozoneized blood transfusion Andrea Barone, Javier Woskoboinic, Aníbal Grangeat, Ricardo Pérez, Ignacio Berra, Guillermo Berra, José Milei. ININCA, Univ Buenos Aires, Argentina Although ozone therapy has been widely used, no attempts have been made to evaluate its protective effects in acute myocardial ischemia. Because of this, a pilot study with a few number of animals was initiated. Under surgical conditions, 200 ml of autologous venous blood, enriched with 20 ml ozone (major autohemotransfusion—MAHT) were infused through the internal jugular vein of adult swines (n = 3), 10 min before the proximal right coronary artery was occluded with a balloon for angioplasty. All animals were sacrificed immediately after having completed the sequence: ST segment elevation, ventricular fibrillation and cardiac arrest. No evident necrotic/ ischemic areas as assessed by tripheniltetrazolium chloride (TTC) were detected and histological sections revealed no damage in myocardial tissue. Conversely, swine infused with plain autologous venous blood (n = 3) and submitted to the same protocol showed preservation in only 81.4 ± 11.6% of the total myocardium, with infarcted/ischemic areas of 14 ± 8.5% and 4.5 ± 3.1%, respectively, (TTC stain), while diffuse monofocal–multifocal necrotic areas were found in microscopical