- Email: [email protected]
INTRAORAL LIPOMAS: A RETROSPECTIVE STUDY OF 43 CASES
OOOO Volume 129, Number 1 Study Design: MEDLINE/PubMed, EMBASE, Web of Science, and Scopus electronic databases were searched to identify relevant articles published up to December 2017. A manual search of bibliographies and reference lists of the included studies were also performed. Results: Initially, 661 studies were gathered and 11 met the inclusion criteria (10 in vitro; 1 in vivo). Evaluated outcomes of the studies were mitotic index, cell proliferation, cell cycle analysis, apoptosis assay, proteins analysis, and morphologic examination. Five (50%) out of 10 in vitro studies demonstrated increased cell proliferation or stimulatory effect; 4 demonstrated inhibition, no proliferation, or lower levels of cell viability and 1 showed a decreased pro-osteoclastogenic potential of the cell lines studied after PBM. The in vivo study concluded that PBM inhibited tumor progression. Laser parameters were poorly described in the majority of the studies. Conclusions: There are a broad variation in the methodologies and controversial results regarding the effects of PBM on HNSCC cells. No clear conclusions could be obtained because of the scarce number of publications. Clinical trials and further in vivo studies are strongly recommended.
FREQUENCY AND PROGNOSTIC IMPORTANCE OF TRANSLOCATION (6;9) (MYBNFIB GENES) IN HEAD AND NECK ADENOID CYSTIC CARCINOMA: A SYSTEMATIC REVIEW. YASMIN DIAS DE ALMEIDA PINTO, SARA FERREIRA DOS SANTOS COSTA, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, ALBINA ALTEMANI, e PABLO AGUSTIN VARGAS, LUCAS GUIMARAES ABREU and, FELIPE PAIVA FONSECA Objective: To carry out a systematic review to address the prevalence and the prognostic potential of translocation (6;9) (MYB-NFIB genes) mutation in head and neck adenoid cystic carcinoma (AdCC). Study Design: Articles published were retrieved from multiple databases using specific search strategies. They were screened and articles included had their data extracted by 2 authors who latter assessed the risk of bias of each study. Quantitative analysis was done to determine the incidence of the translocation. Results: A total of 1107 articles were initially retrieved with 36 remaining for data extraction. The presence of t(6;9)(MYB-NFIB) translocation varied significantly, especially due to methodologic heterogeneity among studies. The meta-analysis revealed a range from 16% to 100% in the prevalence of the translocation. A total of 11 studies attempted to determine the prognostic importance of the mutation, but no study found any significant association with survival rates and only 3 studies observed a significant association with age, sex, tumor location, and the presence of recurrences and metastases. Conclusions: The prevalence of t(6;9)(MYB-NFIB) translocation in head and neck AdCC varies according to the laboratory methods used and the best evidence available demonstrate that t(6;9)(MYB-NFIB) is not a prognostic determinant. Support: CNPq and FAPEMIG.
ORAL CANCER EPIDEMIOLOGY IN PATIENTS ADMITTED TO AN ONCOLOGY HOSPITAL. MILENA THAYS MATIAS DOS
ABSTRACTS
e147
SANTOS, LUCAS EMMANUELL DE MORAIS NEVES, SANDRA APARECIDA MARINHO, SMYRNA LUIZA XIMENES DE SOUZA, SERGIO HENRIQUE GONCALVES ¸ DE CARVALHO, DMITRY JOSE DE SANTANA SARMENTO and, GUSTAVO GOMES AGRIPINO Objective: The aim of this study was to verify the prevalence of oral cancer in patients admitted to a referral oncology hospital. Study Design: Cross-sectional and descriptive study, in which 166 patients diagnosed with cancer were examined. Results: Of the admitted patients, there was a prevalence of males (64.5%) with an average age of 51.7 years and nonwhite color (59%). Among the various types of cancer, acute leukemia (34.9%) was the most prevalent, followed by lymphomas (16.9%), pharynx and larynx (7.8%), mouth (6%), lung (6%), and head and neck cancer (6%). Chemotherapy was the most prescribed (58.4%) therapeutic approach, followed by its association with radiotherapy (18.7%), and only radiotherapy (9.6%). Radiotherapy was the most used treatment for oral cancer. Conclusions: In the evaluated sample, oral cancer was the fourth most prevalent type, predominating for the sixth decade of life, in a ratio of 4 males to 1 female. Radiotherapy was the most-used treatment. Financial support: PIBIC.
GENETIC RISK FACTORS FOR NONSYNDROMIC CLEFT LIP WITH OR WITHOUT CLEFT PALATE IN THE BRAZILIAN POPULATION. RENATO ASSIS MACHADO, EVELYN NAVARRO NOGUEIRA, HERCILIO MARTELLI JUNIOR, SILVIA REGINA DE ALMEIDA REIS, DARLENE CAMATI PERSUHN and, RICARDO D. COLETTA Objective: To evaluate the reported genome-wide risk loci in a Brazilian sample of nonsyndromic cleft lip with or without cleft palate (NSCL§P). Study Design: Single nucleotide polymorphisms (SNPs) rs7552 (2 q24.2), rs8049367 (16 p13.3), rs1880646, rs7406226, rs9891446 (17 p13), rs1588366 (17 q23.2), and rs73039426 (19 q13.11) were genotyped using TaqMan allelic discrimination assays in an ancestry-structured case-control study containing 831 NSCL§P patients and 866 healthy controls. Results: Logistic regression analysis of the singlemarkers revealed rs7552 as a susceptibility risk marker for NSCL§P, yielding an odds ratio (OR) of 1.71 (95% confidence interval [CI]: 1.31-2.24, P = 9 £ 10-6) in the homozygous state. Several SNP-SNP interactions containing rs7552 reached significance after adjustment for multiple tests (both Bonferroni assumption and 1000 permutation test), with the most significant interaction involving the 3-loci among rs7552, rs9891446, and rs73039426 (P = 6.1 £ 10-9 and p1000 permutation = 0.001). Conclusion: Our study is the first to support the association of rs7552 in 2 p24.2 with NSCL§P in the highly admixed Brazilian population.
INTRAORAL LIPOMAS: A RETROSPECTIVE STUDY OF 43 CASES. AUGUSTO CESAR LEAL DA SILVA LEONEL, MATHEUS FERREIRA LINARES, KAMILLA KARLA MAURICIO PASSOS, ELAINE JUDITE DE AMORIM CARVALHO,
ABSTRACTS
e148
JUREMA FREIRE LISBOA DE CASTRO, OSLEI PAES DE ALMEIDA and, DANYEL ELIAS DA CRUZ PEREZ Objective: The aim of this study was to analyze the clinical and histopathologic features of a series of intraoral lipomas (IL). Study Design: The present study was retrospective, observational, and descriptive. All cases of IL diagnosed at an oral pathology laboratory were selected for the study. Clinical data, such as age, sex, location of the lesion, time of evolution, clinical presentation of the lesion, clinical hypothesis of diagnosis, and treatment were collected from the clinical charts. In some cases, to confirm the diagnosis, immunohistochemistry was performed. Results: Forty-three cases of IL were studied. Most of them (55.8%) occurred in women. The mean age was 77.4 years, and the most affected site was the buccal mucosa (51%), followed by the tongue (19%). Histologically, the lesions were defined as classic lipomas (53.4%), fibrolipomas (32.5%), spindle-cell lipomas (9.3%), intramuscular lipoma (2.4%), and sialolipoma (2.4%). Regarding to the clinical hypothesis of diagnosis, lipoma was not considered in 42.9% of the cases. Conclusions: IL are relatively uncommon tumors. In some cases, particularly in spindle-cells lipomas, immunohistochemistry is an important tool to reach the final diagnosis.
TOPICAL APPLICATION OF CHAMOMILE AND CURCUMIN IN ORAL ULCERS: AN EXPERIMENTAL STUDY IN RATS. JULIANE DE QUADROS DE BORTOLLI, MARIANA KLEIN, DIENI TEIXEIRA, GABRIEL LOUZEIRO, KAREN CHERUBINI, MARIA ANTONIA ZANCANARO DE FIGUEIREDO and, FERNANDA GONCALVES ¸ SALUM Objective: To evaluate the effect of topical application of 10% chamomile extract and 2% curcumin gel in the oral ulcers of the rats. Study Design: Thirty-nine Wistar rats were divided into 3 groups: the chamomile group, the curcumin group, and the control group. A 5-mm circular excision wound was made with sterile punch in the tongue of rats; Chamomile extract, curcumin, and vehicle gel were applied 2 times per day for 5 days on the oral ulcers, all animals were euthanized on the sixth day postoperatively. Results: There was no significant difference between the groups in relation to area of residual ulcer (P = .20), newly formed epithelium (P = .97), and intensity of inflammatory infiltrate (P = .724). Conclusions: Chamomile and curcumin had no effect on accelerating the healing of oral ulcers or reducing the inflammatory process.
PREVALENCE OF SALIVARY GLAND LESIONS IN A POPULATION OF RIO DE JANEIRO: AN 8-YEAR RETROSPECTIVE STUDY. CARINA ALMEIDA PIRES, THIAGO MOREIRA PESSOA, RUTH TRAMONTANI RAMOS, FABIO RAMOA PIRES, GERALDO OLIVEIRA SILVA JUNIOR and, MARILIA HEFFER CANTISANO Objective: The aim of this study is to analyze the prevalence of salivary glands lesions in patients attended in the clinic of stomatology, with emphasis on clinical signs and symptoms,
OOOO January 2020 anatomopathologic and imaging diagnoses, in search for the best prognosis. Study Design: This is a retrospective, observational, and longitudinal study. From the analysis of 1736 dental records of the patients attended in the clinic of stomatology, in the period of 2010-2017, 138 cases that present in their definitive diagnosis some lesions of salivary glands were selected. The analyzed information was: Definitive diagnosis, sex, ethnicity, and age. Results: In the present study, 138 cases (7.95%) obtained from dental records show lesions involving salivary glands. Of this number, 92.75% were nonneoplastic in nature and 7.25% were neoplasms; 70.29% were female, and 29.71% were male. The most frequent lesions were mucoceles (32.61%), sialadenitis (20.29%), and Sjogren syndrome (18.12%). Conclusions: According to the data found in this research, it is concluded that the study proposed here is of great clinical relevance; it contributes to data that can lead to a significant reduction in mortality, morbidity, and treatment costs.
INTEROBSERVER AGREEMENT IN ORAL DYSPLASIA GRADING, ASSOCIATED WITH PROTEIN EXPRESSION AND LOSS OF HETEROZIGOSITY. ELIS ANDRADE DE LIMA ZUTIN, YONARA MARIA FREIRE SOARES MARQUES, PATRICIA PIMENTEL DE BARROS, MORUN BERNARDINO NETO, RICARDO ALVES DE MESQUITA, SANDRO ROBERTO VALENTINI and, YASMIN RODARTE CARVALHO Oral leukoplakia (OL) may precede squamous cell carcinoma and its histologic grading is subjective. Loss of heterozygosity (LOH) has been reported as risk predictor of OL malignant transformation. Objective: Correlate histologic and immunohistochemical characteristics of OL and evaluate molecular aspects, aiming at to demonstrate their malignant potential. Study Design: Ninety-six cases of OL were stratified by the World Health Organization (WHO) and Kujan systems by 3 blinded pathologists and correlated to the immunohistochemical biomarkers (IBs) p53, Ki-67, pRb, and p16. In 25 cases, LOH analysis was performed. Results: A moderate concordance among pathologists in both systems (Kendall correlation) was observed. Weak to moderate correlation among IBs was observed, as well as among IBs and histologic grading (Spearman correlation). Staining for p16 showed agreement with all the others IBs. Ki-67 was the most concordant marker in Kujan’s system. The LOH was found in moderate and severe dysplasia for the markers TP53 (17 p13.1) and IFNA (9 p21), in 2 cases each. Conclusions: Greater concordance among pathologists as well as between histologic grading and IBs was demonstrated by Kujan’s system. In OL, p16 can express alone the diagnoses obtained by all others IBs together. LOH could be related to the degree of histopathologic severity and to the greater expression of IBs.
MASS SPECTROMETRY-BASED PROTEOME PROFILE DIFFERENTIATES ADENOID CYSTIC CARCINOMA FROM POLYMORPHOUS ADENOCARCINOMA. FELIPE PAIVA FONSECA, SARA FERREIRA DOS SANTOS COSTA, CAROLINA SOARES CARNEIRO MACEDO, ADRIANA FRANCO PAES LEME, ^ HELDER ANTONIO REBELO PONTES, MANOELA
FREQUENCY AND PROGNOSTIC IMPORTANCE OF TRANSLOCATION (6;9) (MYBNFIB GENES) IN HEAD AND NECK ADENOID CYSTIC CARCINOMA: A SYSTEMATIC REVIEW. YASMIN DIAS DE ALMEIDA PINTO, SARA FERREIRA DOS SANTOS COSTA, BRUNO AUGUSTO BENEVENUTO DE ANDRADE, ALBINA ALTEMANI, e PABLO AGUSTIN VARGAS, LUCAS GUIMARAES ABREU and, FELIPE PAIVA FONSECA Objective: To carry out a systematic review to address the prevalence and the prognostic potential of translocation (6;9) (MYB-NFIB genes) mutation in head and neck adenoid cystic carcinoma (AdCC). Study Design: Articles published were retrieved from multiple databases using specific search strategies. They were screened and articles included had their data extracted by 2 authors who latter assessed the risk of bias of each study. Quantitative analysis was done to determine the incidence of the translocation. Results: A total of 1107 articles were initially retrieved with 36 remaining for data extraction. The presence of t(6;9)(MYB-NFIB) translocation varied significantly, especially due to methodologic heterogeneity among studies. The meta-analysis revealed a range from 16% to 100% in the prevalence of the translocation. A total of 11 studies attempted to determine the prognostic importance of the mutation, but no study found any significant association with survival rates and only 3 studies observed a significant association with age, sex, tumor location, and the presence of recurrences and metastases. Conclusions: The prevalence of t(6;9)(MYB-NFIB) translocation in head and neck AdCC varies according to the laboratory methods used and the best evidence available demonstrate that t(6;9)(MYB-NFIB) is not a prognostic determinant. Support: CNPq and FAPEMIG.
ORAL CANCER EPIDEMIOLOGY IN PATIENTS ADMITTED TO AN ONCOLOGY HOSPITAL. MILENA THAYS MATIAS DOS
ABSTRACTS
e147
SANTOS, LUCAS EMMANUELL DE MORAIS NEVES, SANDRA APARECIDA MARINHO, SMYRNA LUIZA XIMENES DE SOUZA, SERGIO HENRIQUE GONCALVES ¸ DE CARVALHO, DMITRY JOSE DE SANTANA SARMENTO and, GUSTAVO GOMES AGRIPINO Objective: The aim of this study was to verify the prevalence of oral cancer in patients admitted to a referral oncology hospital. Study Design: Cross-sectional and descriptive study, in which 166 patients diagnosed with cancer were examined. Results: Of the admitted patients, there was a prevalence of males (64.5%) with an average age of 51.7 years and nonwhite color (59%). Among the various types of cancer, acute leukemia (34.9%) was the most prevalent, followed by lymphomas (16.9%), pharynx and larynx (7.8%), mouth (6%), lung (6%), and head and neck cancer (6%). Chemotherapy was the most prescribed (58.4%) therapeutic approach, followed by its association with radiotherapy (18.7%), and only radiotherapy (9.6%). Radiotherapy was the most used treatment for oral cancer. Conclusions: In the evaluated sample, oral cancer was the fourth most prevalent type, predominating for the sixth decade of life, in a ratio of 4 males to 1 female. Radiotherapy was the most-used treatment. Financial support: PIBIC.
GENETIC RISK FACTORS FOR NONSYNDROMIC CLEFT LIP WITH OR WITHOUT CLEFT PALATE IN THE BRAZILIAN POPULATION. RENATO ASSIS MACHADO, EVELYN NAVARRO NOGUEIRA, HERCILIO MARTELLI JUNIOR, SILVIA REGINA DE ALMEIDA REIS, DARLENE CAMATI PERSUHN and, RICARDO D. COLETTA Objective: To evaluate the reported genome-wide risk loci in a Brazilian sample of nonsyndromic cleft lip with or without cleft palate (NSCL§P). Study Design: Single nucleotide polymorphisms (SNPs) rs7552 (2 q24.2), rs8049367 (16 p13.3), rs1880646, rs7406226, rs9891446 (17 p13), rs1588366 (17 q23.2), and rs73039426 (19 q13.11) were genotyped using TaqMan allelic discrimination assays in an ancestry-structured case-control study containing 831 NSCL§P patients and 866 healthy controls. Results: Logistic regression analysis of the singlemarkers revealed rs7552 as a susceptibility risk marker for NSCL§P, yielding an odds ratio (OR) of 1.71 (95% confidence interval [CI]: 1.31-2.24, P = 9 £ 10-6) in the homozygous state. Several SNP-SNP interactions containing rs7552 reached significance after adjustment for multiple tests (both Bonferroni assumption and 1000 permutation test), with the most significant interaction involving the 3-loci among rs7552, rs9891446, and rs73039426 (P = 6.1 £ 10-9 and p1000 permutation = 0.001). Conclusion: Our study is the first to support the association of rs7552 in 2 p24.2 with NSCL§P in the highly admixed Brazilian population.
INTRAORAL LIPOMAS: A RETROSPECTIVE STUDY OF 43 CASES. AUGUSTO CESAR LEAL DA SILVA LEONEL, MATHEUS FERREIRA LINARES, KAMILLA KARLA MAURICIO PASSOS, ELAINE JUDITE DE AMORIM CARVALHO,
ABSTRACTS
e148
JUREMA FREIRE LISBOA DE CASTRO, OSLEI PAES DE ALMEIDA and, DANYEL ELIAS DA CRUZ PEREZ Objective: The aim of this study was to analyze the clinical and histopathologic features of a series of intraoral lipomas (IL). Study Design: The present study was retrospective, observational, and descriptive. All cases of IL diagnosed at an oral pathology laboratory were selected for the study. Clinical data, such as age, sex, location of the lesion, time of evolution, clinical presentation of the lesion, clinical hypothesis of diagnosis, and treatment were collected from the clinical charts. In some cases, to confirm the diagnosis, immunohistochemistry was performed. Results: Forty-three cases of IL were studied. Most of them (55.8%) occurred in women. The mean age was 77.4 years, and the most affected site was the buccal mucosa (51%), followed by the tongue (19%). Histologically, the lesions were defined as classic lipomas (53.4%), fibrolipomas (32.5%), spindle-cell lipomas (9.3%), intramuscular lipoma (2.4%), and sialolipoma (2.4%). Regarding to the clinical hypothesis of diagnosis, lipoma was not considered in 42.9% of the cases. Conclusions: IL are relatively uncommon tumors. In some cases, particularly in spindle-cells lipomas, immunohistochemistry is an important tool to reach the final diagnosis.
TOPICAL APPLICATION OF CHAMOMILE AND CURCUMIN IN ORAL ULCERS: AN EXPERIMENTAL STUDY IN RATS. JULIANE DE QUADROS DE BORTOLLI, MARIANA KLEIN, DIENI TEIXEIRA, GABRIEL LOUZEIRO, KAREN CHERUBINI, MARIA ANTONIA ZANCANARO DE FIGUEIREDO and, FERNANDA GONCALVES ¸ SALUM Objective: To evaluate the effect of topical application of 10% chamomile extract and 2% curcumin gel in the oral ulcers of the rats. Study Design: Thirty-nine Wistar rats were divided into 3 groups: the chamomile group, the curcumin group, and the control group. A 5-mm circular excision wound was made with sterile punch in the tongue of rats; Chamomile extract, curcumin, and vehicle gel were applied 2 times per day for 5 days on the oral ulcers, all animals were euthanized on the sixth day postoperatively. Results: There was no significant difference between the groups in relation to area of residual ulcer (P = .20), newly formed epithelium (P = .97), and intensity of inflammatory infiltrate (P = .724). Conclusions: Chamomile and curcumin had no effect on accelerating the healing of oral ulcers or reducing the inflammatory process.
PREVALENCE OF SALIVARY GLAND LESIONS IN A POPULATION OF RIO DE JANEIRO: AN 8-YEAR RETROSPECTIVE STUDY. CARINA ALMEIDA PIRES, THIAGO MOREIRA PESSOA, RUTH TRAMONTANI RAMOS, FABIO RAMOA PIRES, GERALDO OLIVEIRA SILVA JUNIOR and, MARILIA HEFFER CANTISANO Objective: The aim of this study is to analyze the prevalence of salivary glands lesions in patients attended in the clinic of stomatology, with emphasis on clinical signs and symptoms,
OOOO January 2020 anatomopathologic and imaging diagnoses, in search for the best prognosis. Study Design: This is a retrospective, observational, and longitudinal study. From the analysis of 1736 dental records of the patients attended in the clinic of stomatology, in the period of 2010-2017, 138 cases that present in their definitive diagnosis some lesions of salivary glands were selected. The analyzed information was: Definitive diagnosis, sex, ethnicity, and age. Results: In the present study, 138 cases (7.95%) obtained from dental records show lesions involving salivary glands. Of this number, 92.75% were nonneoplastic in nature and 7.25% were neoplasms; 70.29% were female, and 29.71% were male. The most frequent lesions were mucoceles (32.61%), sialadenitis (20.29%), and Sjogren syndrome (18.12%). Conclusions: According to the data found in this research, it is concluded that the study proposed here is of great clinical relevance; it contributes to data that can lead to a significant reduction in mortality, morbidity, and treatment costs.
INTEROBSERVER AGREEMENT IN ORAL DYSPLASIA GRADING, ASSOCIATED WITH PROTEIN EXPRESSION AND LOSS OF HETEROZIGOSITY. ELIS ANDRADE DE LIMA ZUTIN, YONARA MARIA FREIRE SOARES MARQUES, PATRICIA PIMENTEL DE BARROS, MORUN BERNARDINO NETO, RICARDO ALVES DE MESQUITA, SANDRO ROBERTO VALENTINI and, YASMIN RODARTE CARVALHO Oral leukoplakia (OL) may precede squamous cell carcinoma and its histologic grading is subjective. Loss of heterozygosity (LOH) has been reported as risk predictor of OL malignant transformation. Objective: Correlate histologic and immunohistochemical characteristics of OL and evaluate molecular aspects, aiming at to demonstrate their malignant potential. Study Design: Ninety-six cases of OL were stratified by the World Health Organization (WHO) and Kujan systems by 3 blinded pathologists and correlated to the immunohistochemical biomarkers (IBs) p53, Ki-67, pRb, and p16. In 25 cases, LOH analysis was performed. Results: A moderate concordance among pathologists in both systems (Kendall correlation) was observed. Weak to moderate correlation among IBs was observed, as well as among IBs and histologic grading (Spearman correlation). Staining for p16 showed agreement with all the others IBs. Ki-67 was the most concordant marker in Kujan’s system. The LOH was found in moderate and severe dysplasia for the markers TP53 (17 p13.1) and IFNA (9 p21), in 2 cases each. Conclusions: Greater concordance among pathologists as well as between histologic grading and IBs was demonstrated by Kujan’s system. In OL, p16 can express alone the diagnoses obtained by all others IBs together. LOH could be related to the degree of histopathologic severity and to the greater expression of IBs.
MASS SPECTROMETRY-BASED PROTEOME PROFILE DIFFERENTIATES ADENOID CYSTIC CARCINOMA FROM POLYMORPHOUS ADENOCARCINOMA. FELIPE PAIVA FONSECA, SARA FERREIRA DOS SANTOS COSTA, CAROLINA SOARES CARNEIRO MACEDO, ADRIANA FRANCO PAES LEME, ^ HELDER ANTONIO REBELO PONTES, MANOELA