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Inversion inv(4)(p15q26) in a squamous cell carcinoma of the hypopharynx
Inversion inv(4)(p15q26) in a Squamous Cell Carcinoma of the Hypopharynx
There are few cytogenetic studies of solid tumors [1, 2]. Reports of cytogenetic analyses of malignant tumors from the upper aerodigestive tract are restricted to only three squamous cell carcinomas, two of the larynx and one of the tongue [35]. We present here the finding of a single cytogenetic abnormalityinv(4)(p15q26)--in a squamous cell carcinoma of the hypopharynx. The patient, a 71-year-old male smoker, was admitted to the Department of OtoRhino-Laryngology because of hoarseness and dysphagia of 2-3 months' duration.
Figure
1. Partial tumor cell k a r y o t y p e s i b lustrating the inv(4)(p15q26). A r r o w h e a d s indicate breakpoints.
233 ¢~ 1988 Elsevier Science Publishing Co., Inc. 655 Avenue of the Americas, New York, NY 10010
Cancer Genet Cytogenet 36:233- 234 (1988) 0165-4608/88/$03.50
234
Y. lin et al.
He had also d e v e l o p e d d y s p n e a w h e n in a r e c u m b e n t position. At h y p o p h a r y n g o l a r y n g o s c o p y , a p o s t c r i c o i d a l h y p o p h a r y n g e a l t u m o r was found. The patient also had a regional l y m p h n o d e metastasis. A b i o p s y was taken from the p r i m a r y t u m o r and d i v i d e d into two parts, one for c y t o g e n e t i c and the o t h e r for h i s t o p a t h o l o g i c e x a m i n a t i o n . The latter r e v e a l e d a m o d e r a t e l y d i f f e r e n t i a t e d s q u a m o u s cell carcinoma. T h e t u m o r was classified as T 4 N I M O . C h r o m o s o m e p r e p a r a t i o n s w e r e m a d e from s h o r t - t e r m c u l t u r e s i n i t i a t e d after c o l l a g e n a s e disaggregation of the t u m o r b i o p s y (see [3] for d e t a i l e d description). A total of 22 m e t a p h a s e s was p h o t o g r a p h e d and k a r y o t y p e d . A n o r m a l m a l e k a r y o t y p e was f o u n d in two cells. N i n e m e t a p h a s e s had n o n c l o n a l aberrations. In the r e m a i n ing 11 cells, the k a r y o t y p e 46,XY,inv(4)(p15q26) was f o u n d (Fig. 1). A s i m i l a r inv{4) has not b e e n d e s c r i b e d in any o t h e r m a l i g n a n t d i s o r d e r 12]. Until n o w , c y t o g e n e t i c a b n o r m a l i t i e s h a v e not been r e p o r t e d in c a r c i n o m a s of the h y p o p h a r y n x . Nor h a v e s q u a m o u s cell c a r c i n o m a k a r y o t y p e s of a d j a c e n t segm e n t s of the e s o p h a g u s so far been described. Therefore, no data are available for c o m p a r i s o n w i t h the aberration f o u n d in the p r e s e n t case. Supported by grants from the Swedish Cancer Society, The Swedish Work Environment Fund, and the lAP Foundation for Medical Research. Dr. lin is on leave from the Department of Genetics, Cancer Institute, Sun Yat-sen University of Medical Sciences, Guangzhou, People's Republic of China. Y. IlN S. HEIM N. MANDAHL A. BIORKLUND I. WENNERBERG F. MITELMAN
Departments of Clinical Genetics and Oto-Rhino-Laryngology Lund University Hospital S-221 85 Lund, Sweden
REFERENCES 1. Helm S, Mitehnan F (1987): Cancer Cytogenetics. Alan R. Liss, New York. 2. Mitehnan F {1988): Catalog of Chromosome Aberrations in Cancer. 3rd ed, Alan R. Liss, New York. 3, Jin Y. Mandahl N, Helm S, Biorklund A, Wennerberg J, Mitehnan F {1988): Unique karyotypic abnormalities in a squamous cell carcinoma of the larynx. Cancer Genet Cytogenet 30:177 179. 4. ]in Y, Mandahl N, lteim S, Bi6rkhmd A. Weimerberg ], Mitelman F (1988): t(6:7)(q23:p22) as the sole chromosomal anomah, in a vocal cord carcinoma. Cancer Genet Cytogenet 32:305 307. 5. Jin Y, lleim S, Mandah[ N, Bi6rkluud A, Welmerberg J, Mitelmall F (1988): Multiple apparently urn'elated clonal chromosome abnormalities in a squanlous cell caruinoma of the tongue. Cancer Geln;t (]ytogenel 32:93 100.
There are few cytogenetic studies of solid tumors [1, 2]. Reports of cytogenetic analyses of malignant tumors from the upper aerodigestive tract are restricted to only three squamous cell carcinomas, two of the larynx and one of the tongue [35]. We present here the finding of a single cytogenetic abnormalityinv(4)(p15q26)--in a squamous cell carcinoma of the hypopharynx. The patient, a 71-year-old male smoker, was admitted to the Department of OtoRhino-Laryngology because of hoarseness and dysphagia of 2-3 months' duration.
Figure
1. Partial tumor cell k a r y o t y p e s i b lustrating the inv(4)(p15q26). A r r o w h e a d s indicate breakpoints.
233 ¢~ 1988 Elsevier Science Publishing Co., Inc. 655 Avenue of the Americas, New York, NY 10010
Cancer Genet Cytogenet 36:233- 234 (1988) 0165-4608/88/$03.50
234
Y. lin et al.
He had also d e v e l o p e d d y s p n e a w h e n in a r e c u m b e n t position. At h y p o p h a r y n g o l a r y n g o s c o p y , a p o s t c r i c o i d a l h y p o p h a r y n g e a l t u m o r was found. The patient also had a regional l y m p h n o d e metastasis. A b i o p s y was taken from the p r i m a r y t u m o r and d i v i d e d into two parts, one for c y t o g e n e t i c and the o t h e r for h i s t o p a t h o l o g i c e x a m i n a t i o n . The latter r e v e a l e d a m o d e r a t e l y d i f f e r e n t i a t e d s q u a m o u s cell carcinoma. T h e t u m o r was classified as T 4 N I M O . C h r o m o s o m e p r e p a r a t i o n s w e r e m a d e from s h o r t - t e r m c u l t u r e s i n i t i a t e d after c o l l a g e n a s e disaggregation of the t u m o r b i o p s y (see [3] for d e t a i l e d description). A total of 22 m e t a p h a s e s was p h o t o g r a p h e d and k a r y o t y p e d . A n o r m a l m a l e k a r y o t y p e was f o u n d in two cells. N i n e m e t a p h a s e s had n o n c l o n a l aberrations. In the r e m a i n ing 11 cells, the k a r y o t y p e 46,XY,inv(4)(p15q26) was f o u n d (Fig. 1). A s i m i l a r inv{4) has not b e e n d e s c r i b e d in any o t h e r m a l i g n a n t d i s o r d e r 12]. Until n o w , c y t o g e n e t i c a b n o r m a l i t i e s h a v e not been r e p o r t e d in c a r c i n o m a s of the h y p o p h a r y n x . Nor h a v e s q u a m o u s cell c a r c i n o m a k a r y o t y p e s of a d j a c e n t segm e n t s of the e s o p h a g u s so far been described. Therefore, no data are available for c o m p a r i s o n w i t h the aberration f o u n d in the p r e s e n t case. Supported by grants from the Swedish Cancer Society, The Swedish Work Environment Fund, and the lAP Foundation for Medical Research. Dr. lin is on leave from the Department of Genetics, Cancer Institute, Sun Yat-sen University of Medical Sciences, Guangzhou, People's Republic of China. Y. IlN S. HEIM N. MANDAHL A. BIORKLUND I. WENNERBERG F. MITELMAN
Departments of Clinical Genetics and Oto-Rhino-Laryngology Lund University Hospital S-221 85 Lund, Sweden
REFERENCES 1. Helm S, Mitehnan F (1987): Cancer Cytogenetics. Alan R. Liss, New York. 2. Mitehnan F {1988): Catalog of Chromosome Aberrations in Cancer. 3rd ed, Alan R. Liss, New York. 3, Jin Y. Mandahl N, Helm S, Biorklund A, Wennerberg J, Mitehnan F {1988): Unique karyotypic abnormalities in a squamous cell carcinoma of the larynx. Cancer Genet Cytogenet 30:177 179. 4. ]in Y, Mandahl N, lteim S, Bi6rkhmd A. Weimerberg ], Mitelman F (1988): t(6:7)(q23:p22) as the sole chromosomal anomah, in a vocal cord carcinoma. Cancer Genet Cytogenet 32:305 307. 5. Jin Y, lleim S, Mandah[ N, Bi6rkluud A, Welmerberg J, Mitelmall F (1988): Multiple apparently urn'elated clonal chromosome abnormalities in a squanlous cell caruinoma of the tongue. Cancer Geln;t (]ytogenel 32:93 100.