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Investigation of some blood biochemical parameters in sheep naturally infected with theileriosis
Abstracts
spectroscopy. We have chosen 1–5 mg ml− 1 of BSA concentration and the effect of three parameters including temperature, pH and time of incubation on aggregation process. The samples were incubated in temperatures at the range of 40–80 °C and 12, 24, 36 and 48 h of agitation. Results: Results confirm the developing of the fibrils and oligomers by progressing the time of incubation. The optimum concentration, pH and temperature of fibrilogenesis were achieved at 5 mg/ml, 3.3 and 65 °C, respectively. Conclusion: The process optimized in 48 h agitation (5 mg/ml of BSA in 65 °C at 3.3 buffer pH). According to this study the best condition evaluated for use was producing fibrils as bio-nanomaterials in new aspect of protein aggregation. Keywords: Bovine serum albumin, Aggregation, Fibrillation, Optimization
doi:10.1016/j.clinbiochem.2011.08.329
Poster – [A-10-265-3] Investigation of some blood biochemical parameters in sheep naturally infected with theileriosis Hasan Baghishani, Reza Razmi Gholam, Saeed Yaghfoori, Amin Ahmadi Dezaki Ferdowsi University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (H. Baghishani), [email protected] (R. Razmi Gholam), [email protected] (S. Yaghfoori), [email protected] (A. Ahmadi Dezaki) Introduction: Theileriosis is one of the most important haemoparasitic diseases in ruminants and have long been considered as a major problem to efficient sheep and goats production in Iran. Determination of blood biochemical parameters is an important part of the clinical laboratory diagnostic tools available to veterinarians to assess health and disease in animal species. The present study was conducted to investigate the effect of sheep theileriosis on some blood biochemical parameters. Methods: Thirty adult fat-tailed sheep suffering from theileriosis were selected on the basis of clinical examination and positive peripheral blood smears, and twenty clinically healthy animals without parasitaemia (according to the blood smears) served as controls. Plasma biochemical parameters were assayed using colorimetric methods. Results: Biochemical analysis of infected cases revealed a significant (P < 0.05) decrease in blood glucose and albumin, in addition to a non-significant decrease in total protein and creatinine concentrations when compared to the uninfected group. On the other hand, a significant increase of urea, total bilirubin, aspartate aminotransferase and lactate dehydrogenase, in addition to a nonsignificant rise in triglyceride, cholesterol, uric acid, alanine aminotransferase, alkaline phosphatise and creatine kinase levels was found in infected group in comparison to controls. Conclusion: This study led to conclude that theileriosis in sheep is associated with some alterations in blood biochemical parameters. Thus, measuring these changes may provide help to understand the disease pathogenesis and could be used as a tool for diagnosis, prognosis and evaluation of the therapy applied. Keywords: Blood biochemical parameters, Sheep, Theileriosis doi:10.1016/j.clinbiochem.2011.08.330
S137
Poster – [A-10-267-1] Assassment of serum levels of LH and FSH in patient with polycystic ovary syndrome Mona Asadi Mashhad, Iran E-mail address: [email protected] Introduction: This study was Polycystic Ovary Syndrome (POCS) is one of the most common endocrinopathies in reproductive age women. Endocrine disorders that is common in this patient include anormal level of gonadotropin hormone, TSH, prolactin and Insulin resistance. This study was designed to assess the level of LH, FSH and proportion of LH/FSH in PCOS patient. Materials and methods: The serum samples of 258 that 65 of them had PCOS and 193 were healthy were collected at the third day of menstrual cycle. By using of RIA technics the level of PRL determined and then the results by statistical T test and Simple test and SPSS analysis (18 version) were analyzed. P < 0.005 as significant level was considerd. Results: The mean age of control group was 32 years old and patient was 27. Significant statistic correlation had shown between age and incidence of syndrome (p < 0.001). There is a significant correlation between the serum level of LH and disease (p < 0.0). Significant relation between FSH and incidence of syndrome had shown, too (p < 0.002). Proportion of LH/FSH in compare with control group had shown a significant relation (p < 0.0). Conclusion: The prevalence of this disease is more commonin younger women. The level of LH and proportion of LH/FSH in this disease is increased and the level of FSH is decreased. Keywords: LH, FSH, Polycistic ovary syndrome, Age
doi:10.1016/j.clinbiochem.2011.08.331
Poster – [A-10-270-1] Effect of food restriction and postprandial hyperglycemia control on dyslipidemia in diabetic rats Azam Alavi nezhad Neuroscience Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] Introduction: In the past, the main cause of vascular disorders in diabetes was considered to be chronic hyperglycemia, but nowadays the role of postprandial hyperglycemia in inducing vascular disorders is more prominent. Purpose this research is study the effect of food restriction and postprandial hyperglycemia control on vascular disorders in diabetes. Method: Groups were included: control, diabetic, diabetic + food restriction, diabetic treated with NPH insulin, diabetic treated with NPH insulin + FR, diabetic treated with 70/30 insulin, diabetic treated with 70/30 insulin + FR. Diabetes was induced with subcutaneous injection of 60 mg/kg streptozocin. To control postprandial hyperglycemia, both types of insulins were injected 2 units in the morning before giving the food. In addition, the amount of food for diabetic rats with food restriction was considered as the amount of food for control rats. Serum glucose, total cholesterol and triglyceride concentrations at the end of the first, second and third months of the exam were measured. Results: Serum glucose levels in all groups of diabetic rats showed a significant increase compared to control group (p < 0.001). Total cholesterol measured at three times was not significantly different in diabetic groups versus the control group. Triglyceride levels were significantly increased in diabetic group than other groups (p< 0.001).
spectroscopy. We have chosen 1–5 mg ml− 1 of BSA concentration and the effect of three parameters including temperature, pH and time of incubation on aggregation process. The samples were incubated in temperatures at the range of 40–80 °C and 12, 24, 36 and 48 h of agitation. Results: Results confirm the developing of the fibrils and oligomers by progressing the time of incubation. The optimum concentration, pH and temperature of fibrilogenesis were achieved at 5 mg/ml, 3.3 and 65 °C, respectively. Conclusion: The process optimized in 48 h agitation (5 mg/ml of BSA in 65 °C at 3.3 buffer pH). According to this study the best condition evaluated for use was producing fibrils as bio-nanomaterials in new aspect of protein aggregation. Keywords: Bovine serum albumin, Aggregation, Fibrillation, Optimization
doi:10.1016/j.clinbiochem.2011.08.329
Poster – [A-10-265-3] Investigation of some blood biochemical parameters in sheep naturally infected with theileriosis Hasan Baghishani, Reza Razmi Gholam, Saeed Yaghfoori, Amin Ahmadi Dezaki Ferdowsi University of Mashhad, Mashhad, Iran E-mail addresses: [email protected] (H. Baghishani), [email protected] (R. Razmi Gholam), [email protected] (S. Yaghfoori), [email protected] (A. Ahmadi Dezaki) Introduction: Theileriosis is one of the most important haemoparasitic diseases in ruminants and have long been considered as a major problem to efficient sheep and goats production in Iran. Determination of blood biochemical parameters is an important part of the clinical laboratory diagnostic tools available to veterinarians to assess health and disease in animal species. The present study was conducted to investigate the effect of sheep theileriosis on some blood biochemical parameters. Methods: Thirty adult fat-tailed sheep suffering from theileriosis were selected on the basis of clinical examination and positive peripheral blood smears, and twenty clinically healthy animals without parasitaemia (according to the blood smears) served as controls. Plasma biochemical parameters were assayed using colorimetric methods. Results: Biochemical analysis of infected cases revealed a significant (P < 0.05) decrease in blood glucose and albumin, in addition to a non-significant decrease in total protein and creatinine concentrations when compared to the uninfected group. On the other hand, a significant increase of urea, total bilirubin, aspartate aminotransferase and lactate dehydrogenase, in addition to a nonsignificant rise in triglyceride, cholesterol, uric acid, alanine aminotransferase, alkaline phosphatise and creatine kinase levels was found in infected group in comparison to controls. Conclusion: This study led to conclude that theileriosis in sheep is associated with some alterations in blood biochemical parameters. Thus, measuring these changes may provide help to understand the disease pathogenesis and could be used as a tool for diagnosis, prognosis and evaluation of the therapy applied. Keywords: Blood biochemical parameters, Sheep, Theileriosis doi:10.1016/j.clinbiochem.2011.08.330
S137
Poster – [A-10-267-1] Assassment of serum levels of LH and FSH in patient with polycystic ovary syndrome Mona Asadi Mashhad, Iran E-mail address: [email protected] Introduction: This study was Polycystic Ovary Syndrome (POCS) is one of the most common endocrinopathies in reproductive age women. Endocrine disorders that is common in this patient include anormal level of gonadotropin hormone, TSH, prolactin and Insulin resistance. This study was designed to assess the level of LH, FSH and proportion of LH/FSH in PCOS patient. Materials and methods: The serum samples of 258 that 65 of them had PCOS and 193 were healthy were collected at the third day of menstrual cycle. By using of RIA technics the level of PRL determined and then the results by statistical T test and Simple test and SPSS analysis (18 version) were analyzed. P < 0.005 as significant level was considerd. Results: The mean age of control group was 32 years old and patient was 27. Significant statistic correlation had shown between age and incidence of syndrome (p < 0.001). There is a significant correlation between the serum level of LH and disease (p < 0.0). Significant relation between FSH and incidence of syndrome had shown, too (p < 0.002). Proportion of LH/FSH in compare with control group had shown a significant relation (p < 0.0). Conclusion: The prevalence of this disease is more commonin younger women. The level of LH and proportion of LH/FSH in this disease is increased and the level of FSH is decreased. Keywords: LH, FSH, Polycistic ovary syndrome, Age
doi:10.1016/j.clinbiochem.2011.08.331
Poster – [A-10-270-1] Effect of food restriction and postprandial hyperglycemia control on dyslipidemia in diabetic rats Azam Alavi nezhad Neuroscience Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] Introduction: In the past, the main cause of vascular disorders in diabetes was considered to be chronic hyperglycemia, but nowadays the role of postprandial hyperglycemia in inducing vascular disorders is more prominent. Purpose this research is study the effect of food restriction and postprandial hyperglycemia control on vascular disorders in diabetes. Method: Groups were included: control, diabetic, diabetic + food restriction, diabetic treated with NPH insulin, diabetic treated with NPH insulin + FR, diabetic treated with 70/30 insulin, diabetic treated with 70/30 insulin + FR. Diabetes was induced with subcutaneous injection of 60 mg/kg streptozocin. To control postprandial hyperglycemia, both types of insulins were injected 2 units in the morning before giving the food. In addition, the amount of food for diabetic rats with food restriction was considered as the amount of food for control rats. Serum glucose, total cholesterol and triglyceride concentrations at the end of the first, second and third months of the exam were measured. Results: Serum glucose levels in all groups of diabetic rats showed a significant increase compared to control group (p < 0.001). Total cholesterol measured at three times was not significantly different in diabetic groups versus the control group. Triglyceride levels were significantly increased in diabetic group than other groups (p< 0.001).