- Email: [email protected]
Investigation of the effects of interferon-γ on serum interleukins-4 and 6, disease severity and quality of life in patients with atopic dermatitis
S152
Abstracts
Poster – [A-10-602-4] Investigation of the effects of interferon-γ on serum interleukins-4 and 6, disease severity and quality of life in patients with atopic dermatitis Sahebkar Amirhossein, Panahi Yunes, Beiraghdar Fatemeh, Amiri Mojtaba, Madanchi Nima Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (S. Amirhossein) Introduction: Atopic dermatitis (AD) is a chronic, relapsing and inflammatory disease which is characterized by eczema and pruritic skin symptoms. In spite of its high prevalence and its adverse impact on the quality of life, there is as yet no standard treatment for AD. In the present study, the effect of treatment with interferon-γ was investigated in AD patients. Methods: Eighteen patients (12 males and 6 females) were treated with subcutaneous IFN-γ (50 μg/m2; 3 times per week) for one month. Serum concentrations of interleukin-4 (IL-4), IL-6 and IgE, together with disease severity and quality of life were evaluated at baseline and at the end of trial. Assessment of quality of life was performed using Dermatology Life Quality Index (DLQI). The severity of AD was evaluated using SCORAD index which comprises 3 subsections: SCORAD-A (indicative of the extent of disorder), SCORAD-B (indicative of the intensity of the disorder) and SCORAD-C (indicative of subjective symptoms including itch and sleeplessness). Results: Administration of IFN-γ was associated with significant decreases in serum IL-4 (p < 0.001) and IL-6 (p < 0.001) concentrations. DLQI (p < 0.001), SCORAD-A and SCORAD-B indexes were also significantly improved by the end of trial. However, no significant difference in SCORAD-C index and serum IgE concentrations was observed (p > 0.05). Conclusion: Although no significant effect was observed on serum IgE, IFN-γ could ameliorate the quality of life and reduce the extent and intensity of disease in AD patients. These favorable effects could be attributed to the impact of IFN-γ in decreasing serum IL-4 and IL-6 concentrations. Keywords: Interfon-γ, Atopic dermatitis, Quality of life, Interleukins, IgE, SCORAD doi:10.1016/j.clinbiochem.2011.08.376
Poster – [A-10-605-1] Folate, vitamin B12 and homocystine status in the elderly in North-eastern Iran Manavifar Lida Mashhad, Iran E-mail address: [email protected] Introduction: With respect to prevalence of folate and cobalamin deficiency in old people, determining a cut off point for low and marginal levels of folate and cobalamin deficiency before appearance of anemia or neurocognitive presentations in elderly is necessary. Materials and Methods: In this cross-sectional study, 280 persons over 65 years old in the twelve regions of Mashhad city admitted to Emam Reza (p.b.up) hospital. They were physically examined by a general practitioner. Other data were obtained by questionaire. Serum cobalamin and folate measured by RIA method. Seventy six persons, who had cobalamin 120–450 pg/ml and folate 1.5-7 ng/ml without any homocystein (Hcy) confounders (creatinin > 1.5 g/dl, cigarette smoking and consuming some drugs), measured for serum homocystein by Elisa method. All collected data statistically analyzed by spss software.
Results: In considerated levels of folate and cobalamin deficiency, 48.7% of old people had hyperhomocysteinemia. Hcy was inversely correlated with cobalamin (p = 0.001) and with folate bellow cut off point (p = 0.044). According to Hcy > 15 mol/lμ, cut off point for cobalamin and folate deficiency was <330 pg/ml and <6.5 ng/ml, respectively. Prevalence of cobalamin deficiency in low levels (<122 pg/ml) was 22.7% and in marginal levels (122–330 pg/ml) was 51.8%. Prevalence of folate deficiency in low levels (<3 ng/ml) was 16.7% and in marginal levels (<6.5 ng/ml) was 64.2%. Conclusion: According to definition of cut off point, prevalence of cobalamin and folate deficiency especially in marginal levels is higher than that is recorded. It is recommended that before appearance the sign and symptoms of deficiencies and other risks of hyperhomocysteinemia, old people are screened for cobalamin and folate status at appropriate intervals. Keywords: Cobalamin(V.B12), Folate, Hemocystein, Old peaple doi:10.1016/j.clinbiochem.2011.08.377
Poster — [A-10-622-1] Effect of statin therapy on serum trace element status in dyslipidemic patients: Results of a placebo-controlled cross-over trail Asad Zadeh Aghdaei Hamida, Abbas Heshmatib, Farzaneh Norouzic, Shima Tavallaeid, Majid Ghayour Mobarhane, Mohsen Mohebatia a Department of Laboratory, Razavi Hospital, Mashhad, Iran b Paradise Daneshgah, Azadi Square, Khorasan Razavi, Mashhad, Iran c Mashhad University of Medical Sciences, Mashhad, Iran d BuAli Research Institute, BuAli Square, Mashhad, Iran e Biochemistry and Nutrition Research Center, Faculty of Medicine, Mashhad, Iran E-mail addresses: [email protected] (A. Heshmati), [email protected] (F. Norouzi), [email protected] (S. Tavallaei), [email protected] (M. Ghayour Mobarhan) Introduction: Statins are widely used in the management of coronary risk by their efficacy in reducing LDL and their pleiotropic effects. Trace elements such as Zinc and Copper have potential role in atherosclerosis. We wished to investigate the effects of simvastatin on serum Zinc, copper, Zinc/Copper superoxide dismutase (SOD) and ceruloplasmin (Cp) in dyslipidemic patients. Methods: We treated 102 dyslipidemic individuals who had not used statins with simvastatin or placebo in a double-blind, cross-over, placebo-controlled trial for a period of 4 weeks with 2 weeks wash out. Lipid profile, FBS and serum concentrations of Zn, Cu, SOD and Cp were measured before and after each treatment period. Results: Treatment with simvastatin was associated with significant reductions in TC, LDL (p < 0.001), TG (p < 0.001) and hs-CRP (p < 0.05) in both groups. A significant correlation between serum concentrations of Cp and total cholesterol (p < 0.05) was observed. However statin therapy did not change serum concentrations of Zn, Cu, Cp and the activity of SOD in statin-placebo group and placebostatin group (p < 0.05). Conclusion: We found that simvastatin therapy for 4 weeks improved lipid profile. However there was no significantly change on serum level of Zn, Cu and Cp. These results may be affected by the class of statin, the dose which was used and the period of administrating. Therefore, conducting larger multicenter trials is recommended. Keywords: Statins, Cu, Zn, SoD Cu/Zn, Cp, Se doi:10.1016/j.clinbiochem.2011.08.378
Abstracts
Poster – [A-10-602-4] Investigation of the effects of interferon-γ on serum interleukins-4 and 6, disease severity and quality of life in patients with atopic dermatitis Sahebkar Amirhossein, Panahi Yunes, Beiraghdar Fatemeh, Amiri Mojtaba, Madanchi Nima Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (S. Amirhossein) Introduction: Atopic dermatitis (AD) is a chronic, relapsing and inflammatory disease which is characterized by eczema and pruritic skin symptoms. In spite of its high prevalence and its adverse impact on the quality of life, there is as yet no standard treatment for AD. In the present study, the effect of treatment with interferon-γ was investigated in AD patients. Methods: Eighteen patients (12 males and 6 females) were treated with subcutaneous IFN-γ (50 μg/m2; 3 times per week) for one month. Serum concentrations of interleukin-4 (IL-4), IL-6 and IgE, together with disease severity and quality of life were evaluated at baseline and at the end of trial. Assessment of quality of life was performed using Dermatology Life Quality Index (DLQI). The severity of AD was evaluated using SCORAD index which comprises 3 subsections: SCORAD-A (indicative of the extent of disorder), SCORAD-B (indicative of the intensity of the disorder) and SCORAD-C (indicative of subjective symptoms including itch and sleeplessness). Results: Administration of IFN-γ was associated with significant decreases in serum IL-4 (p < 0.001) and IL-6 (p < 0.001) concentrations. DLQI (p < 0.001), SCORAD-A and SCORAD-B indexes were also significantly improved by the end of trial. However, no significant difference in SCORAD-C index and serum IgE concentrations was observed (p > 0.05). Conclusion: Although no significant effect was observed on serum IgE, IFN-γ could ameliorate the quality of life and reduce the extent and intensity of disease in AD patients. These favorable effects could be attributed to the impact of IFN-γ in decreasing serum IL-4 and IL-6 concentrations. Keywords: Interfon-γ, Atopic dermatitis, Quality of life, Interleukins, IgE, SCORAD doi:10.1016/j.clinbiochem.2011.08.376
Poster – [A-10-605-1] Folate, vitamin B12 and homocystine status in the elderly in North-eastern Iran Manavifar Lida Mashhad, Iran E-mail address: [email protected] Introduction: With respect to prevalence of folate and cobalamin deficiency in old people, determining a cut off point for low and marginal levels of folate and cobalamin deficiency before appearance of anemia or neurocognitive presentations in elderly is necessary. Materials and Methods: In this cross-sectional study, 280 persons over 65 years old in the twelve regions of Mashhad city admitted to Emam Reza (p.b.up) hospital. They were physically examined by a general practitioner. Other data were obtained by questionaire. Serum cobalamin and folate measured by RIA method. Seventy six persons, who had cobalamin 120–450 pg/ml and folate 1.5-7 ng/ml without any homocystein (Hcy) confounders (creatinin > 1.5 g/dl, cigarette smoking and consuming some drugs), measured for serum homocystein by Elisa method. All collected data statistically analyzed by spss software.
Results: In considerated levels of folate and cobalamin deficiency, 48.7% of old people had hyperhomocysteinemia. Hcy was inversely correlated with cobalamin (p = 0.001) and with folate bellow cut off point (p = 0.044). According to Hcy > 15 mol/lμ, cut off point for cobalamin and folate deficiency was <330 pg/ml and <6.5 ng/ml, respectively. Prevalence of cobalamin deficiency in low levels (<122 pg/ml) was 22.7% and in marginal levels (122–330 pg/ml) was 51.8%. Prevalence of folate deficiency in low levels (<3 ng/ml) was 16.7% and in marginal levels (<6.5 ng/ml) was 64.2%. Conclusion: According to definition of cut off point, prevalence of cobalamin and folate deficiency especially in marginal levels is higher than that is recorded. It is recommended that before appearance the sign and symptoms of deficiencies and other risks of hyperhomocysteinemia, old people are screened for cobalamin and folate status at appropriate intervals. Keywords: Cobalamin(V.B12), Folate, Hemocystein, Old peaple doi:10.1016/j.clinbiochem.2011.08.377
Poster — [A-10-622-1] Effect of statin therapy on serum trace element status in dyslipidemic patients: Results of a placebo-controlled cross-over trail Asad Zadeh Aghdaei Hamida, Abbas Heshmatib, Farzaneh Norouzic, Shima Tavallaeid, Majid Ghayour Mobarhane, Mohsen Mohebatia a Department of Laboratory, Razavi Hospital, Mashhad, Iran b Paradise Daneshgah, Azadi Square, Khorasan Razavi, Mashhad, Iran c Mashhad University of Medical Sciences, Mashhad, Iran d BuAli Research Institute, BuAli Square, Mashhad, Iran e Biochemistry and Nutrition Research Center, Faculty of Medicine, Mashhad, Iran E-mail addresses: [email protected] (A. Heshmati), [email protected] (F. Norouzi), [email protected] (S. Tavallaei), [email protected] (M. Ghayour Mobarhan) Introduction: Statins are widely used in the management of coronary risk by their efficacy in reducing LDL and their pleiotropic effects. Trace elements such as Zinc and Copper have potential role in atherosclerosis. We wished to investigate the effects of simvastatin on serum Zinc, copper, Zinc/Copper superoxide dismutase (SOD) and ceruloplasmin (Cp) in dyslipidemic patients. Methods: We treated 102 dyslipidemic individuals who had not used statins with simvastatin or placebo in a double-blind, cross-over, placebo-controlled trial for a period of 4 weeks with 2 weeks wash out. Lipid profile, FBS and serum concentrations of Zn, Cu, SOD and Cp were measured before and after each treatment period. Results: Treatment with simvastatin was associated with significant reductions in TC, LDL (p < 0.001), TG (p < 0.001) and hs-CRP (p < 0.05) in both groups. A significant correlation between serum concentrations of Cp and total cholesterol (p < 0.05) was observed. However statin therapy did not change serum concentrations of Zn, Cu, Cp and the activity of SOD in statin-placebo group and placebostatin group (p < 0.05). Conclusion: We found that simvastatin therapy for 4 weeks improved lipid profile. However there was no significantly change on serum level of Zn, Cu and Cp. These results may be affected by the class of statin, the dose which was used and the period of administrating. Therefore, conducting larger multicenter trials is recommended. Keywords: Statins, Cu, Zn, SoD Cu/Zn, Cp, Se doi:10.1016/j.clinbiochem.2011.08.378