- Email: [email protected]
Involvement of chromosome 12 in well differentiated liposarcoma
Abstracts
12 7
NEW CASE OF BENIGN LIPOMA WITH t(2;12)(p23;q14): CHROMOSOME BAND 2p21-p23 IS A SITE OF NONRANDOM REARRANGEMENT F. MUGNERET, F. PEDEUTOUR, A. DEBADIER, I. SIDANER, C. TURC-CAREL * Laboratory of Cytogenetics, Faculty of Medicine 21033 DIJON FRANCE + LGMCH, CNRS, URA 1462, Faculty of Medicine 06107 NICE FRANCE In this study, we report a new case of benign lipoma in a 70 year-old woman with a t(2;12)(p23;q14). In lipomas, consistent of rearrangements of chromosome 12 at bands 12q13-q14 have been observed. In a recent study (1), the chromosome 12 was rearranged the most frequent with chromosomes 1, 2, 3 and 21. Five cases were rearranged with 2p21p23; our observation confirm the recurrent and nonrandom nature of the implication of the chromosome 2 in lipomas. We have used non isotopic in situ hybridization for the mapping of various probes on chromosome 12; these included: COL2AI, GLI, pYNH15. The two genes and the anonymous DNA segment - are localized on derivative chromosome 12. (1) SREEKANTIAIAH C. and al, Cancer Research 1991, 51, 422-433
INVOLVEMENT OF CHROMOSOME 12 IN WELL DIFFERENTIATED LIPOSARCOMA N Florence Pedeutour (1), Agn~s Lamouroux (1), Jean Michel Coindre (2), Fran~oise Collin (3), Colette Bouchot (1), Claude Turc Carel 1. LGMCH, CNRS URA 1462, Facult~ de M~decine 06107 NICE 2. Fondation Bergoni~, 33076 BORDEAUX 3. Centre Georges-Frangois Leclerc, 21034 DIJON, FRANCE. Involvement of chromosome 12 in reciprocal translocations occur as non random and specific changes in benign lipoma and myxoid liposarcoma. In other histological subtypes of liposarcoma, different chromosomal changes are found. They are not as specific as the ones mentioned above, but they exhibit some consistency. These changes include giant marker(s), and/or ring(s) chromosomes. The definition of the origin(s) of such marker chromosomes is more often impossible to ascertain by standard cytogenetic banding techniques. We have used fluorescent in situ hybridization with centromeric and whole chromosome painting probes to tentatively characterize such chromosome markers in two cases of well differentiated liposarcoma (WDLPS). One was a WDLPS lipoma-like. The sole abnormality was a supernumerary giant chromosome with abnormal banded regions. Sequences of chromosome 12 were shown on the entire length of the marker, but alternated with sequences from other chromosomes. The second case was a WDLPS with supernumerary ring chromosomes (2 to 4). Every ring was shown to contain chromosome 12 sequences. With in mind the specific involvement of the 12q13-q14 region in lipoma and myxoid liposarcoma, is the presence of chromosome 12 sequences in another subtype of liposarcoma just a coincidence ? or may we postulate that a same gene located at the 12q13-q14 region is specifically involved in adipocyte transformation, when rearranged or deregulated following translocation or amplification or ring formation ? To answer to these questions, additional investigations are necessary 1/at the chromosomal level to more precisely know the genetic content of the 12 sequences found in WDLPS, using single copy probes of genes located at the 12q13-q14 region (in progress), 2/ at the molecular level.
12 7
NEW CASE OF BENIGN LIPOMA WITH t(2;12)(p23;q14): CHROMOSOME BAND 2p21-p23 IS A SITE OF NONRANDOM REARRANGEMENT F. MUGNERET, F. PEDEUTOUR, A. DEBADIER, I. SIDANER, C. TURC-CAREL * Laboratory of Cytogenetics, Faculty of Medicine 21033 DIJON FRANCE + LGMCH, CNRS, URA 1462, Faculty of Medicine 06107 NICE FRANCE In this study, we report a new case of benign lipoma in a 70 year-old woman with a t(2;12)(p23;q14). In lipomas, consistent of rearrangements of chromosome 12 at bands 12q13-q14 have been observed. In a recent study (1), the chromosome 12 was rearranged the most frequent with chromosomes 1, 2, 3 and 21. Five cases were rearranged with 2p21p23; our observation confirm the recurrent and nonrandom nature of the implication of the chromosome 2 in lipomas. We have used non isotopic in situ hybridization for the mapping of various probes on chromosome 12; these included: COL2AI, GLI, pYNH15. The two genes and the anonymous DNA segment - are localized on derivative chromosome 12. (1) SREEKANTIAIAH C. and al, Cancer Research 1991, 51, 422-433
INVOLVEMENT OF CHROMOSOME 12 IN WELL DIFFERENTIATED LIPOSARCOMA N Florence Pedeutour (1), Agn~s Lamouroux (1), Jean Michel Coindre (2), Fran~oise Collin (3), Colette Bouchot (1), Claude Turc Carel 1. LGMCH, CNRS URA 1462, Facult~ de M~decine 06107 NICE 2. Fondation Bergoni~, 33076 BORDEAUX 3. Centre Georges-Frangois Leclerc, 21034 DIJON, FRANCE. Involvement of chromosome 12 in reciprocal translocations occur as non random and specific changes in benign lipoma and myxoid liposarcoma. In other histological subtypes of liposarcoma, different chromosomal changes are found. They are not as specific as the ones mentioned above, but they exhibit some consistency. These changes include giant marker(s), and/or ring(s) chromosomes. The definition of the origin(s) of such marker chromosomes is more often impossible to ascertain by standard cytogenetic banding techniques. We have used fluorescent in situ hybridization with centromeric and whole chromosome painting probes to tentatively characterize such chromosome markers in two cases of well differentiated liposarcoma (WDLPS). One was a WDLPS lipoma-like. The sole abnormality was a supernumerary giant chromosome with abnormal banded regions. Sequences of chromosome 12 were shown on the entire length of the marker, but alternated with sequences from other chromosomes. The second case was a WDLPS with supernumerary ring chromosomes (2 to 4). Every ring was shown to contain chromosome 12 sequences. With in mind the specific involvement of the 12q13-q14 region in lipoma and myxoid liposarcoma, is the presence of chromosome 12 sequences in another subtype of liposarcoma just a coincidence ? or may we postulate that a same gene located at the 12q13-q14 region is specifically involved in adipocyte transformation, when rearranged or deregulated following translocation or amplification or ring formation ? To answer to these questions, additional investigations are necessary 1/at the chromosomal level to more precisely know the genetic content of the 12 sequences found in WDLPS, using single copy probes of genes located at the 12q13-q14 region (in progress), 2/ at the molecular level.