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IP006 The influence of uremia and hemodialysis on neutrophil phagocytosis and antimicrobial killing
Wissenschaftliches Programm 55. DGHM-Tagung 29. September-1. Oktober 2003 in Dresden Abstracts - Poster
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The influence of uremia and hemodialysis on neutrophil phagocytosis and antimicrobial killing Anding-Rost, K.1; Gross, p.2; Rost, J.M.3; Wei6bach, L.1; Ruppelt, A } ; Jacobs, E. 1
~TU Dresden; Medical Microbiology 2TU Dresden; Nephrology 3Max Planck-Institute; Physics of Complex Systems Background: Neutrophil functions in hemodialysis (HD) patients are altered by uremia and by hemodialysis procedure. We investigate details of the neutrophil dysfunction since its nature and origin is not well understood. This is reflected by conflicting results about neutrophil phagocytosis activity and by scarce data on the neutrophil killing capability in HD-patients. Methods: Using a flowcytometric test system we have measured simultaneously phagocytosis, antimicrobial killing of yeast by neutrophils and the production of reactive oxygen species (ROS) of neutrophils in 117 whole-blood samples of healthy controls and in 50 pre- and 50 postdialysis samples of HD-patients, half of them with diabetes mellitus (DM). We have constructed a model to account for the dependence on the stimulus-to-cell ratio and obtain means for phagocytosis and killing at different incubation times. Results: (i) HD-patients have significantly lower neutrophil killing (20%) than healthy controls. (ii) Dialysis improves the killing capability by 10-15%, after dialysis the killing activity remains significantly (10%) below that of the controls. (iii) The percentage of neutrophils, which exhibit phagocytosis and produce ROS does not differ significantly between HD-patients and healthy controls. (iv) Age has no significant influence on phagocytosis and killing. Conclusion: The neutrophil killing capability is reduced in HDpatients while the amount of neutrophils which phagocyte and produce ROS remains unchanged. Functional impairment of uremic neutrophils is therefore mainly a result of their reduced capability to kill microorganisms intracellularly.
http://www.dghm.org
208
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The influence of uremia and hemodialysis on neutrophil phagocytosis and antimicrobial killing Anding-Rost, K.1; Gross, p.2; Rost, J.M.3; Wei6bach, L.1; Ruppelt, A } ; Jacobs, E. 1
~TU Dresden; Medical Microbiology 2TU Dresden; Nephrology 3Max Planck-Institute; Physics of Complex Systems Background: Neutrophil functions in hemodialysis (HD) patients are altered by uremia and by hemodialysis procedure. We investigate details of the neutrophil dysfunction since its nature and origin is not well understood. This is reflected by conflicting results about neutrophil phagocytosis activity and by scarce data on the neutrophil killing capability in HD-patients. Methods: Using a flowcytometric test system we have measured simultaneously phagocytosis, antimicrobial killing of yeast by neutrophils and the production of reactive oxygen species (ROS) of neutrophils in 117 whole-blood samples of healthy controls and in 50 pre- and 50 postdialysis samples of HD-patients, half of them with diabetes mellitus (DM). We have constructed a model to account for the dependence on the stimulus-to-cell ratio and obtain means for phagocytosis and killing at different incubation times. Results: (i) HD-patients have significantly lower neutrophil killing (20%) than healthy controls. (ii) Dialysis improves the killing capability by 10-15%, after dialysis the killing activity remains significantly (10%) below that of the controls. (iii) The percentage of neutrophils, which exhibit phagocytosis and produce ROS does not differ significantly between HD-patients and healthy controls. (iv) Age has no significant influence on phagocytosis and killing. Conclusion: The neutrophil killing capability is reduced in HDpatients while the amount of neutrophils which phagocyte and produce ROS remains unchanged. Functional impairment of uremic neutrophils is therefore mainly a result of their reduced capability to kill microorganisms intracellularly.
http://www.dghm.org
208