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Isolation and characterization of halophilic bacteria from Çamaltı Saltern Turkey
New Biotechnology · Volume 25S · September 2009
of energy and carbon Yarrowia lipolytica is an unique strictly aerobic yeast with the ability to degrade very efficiently hydrophobic substrates such as n-alkanes, fatty acids, fats and oils for which it has specific metabolic pathways. So, Y. lipolytica is emerging as a promising agent in crude oil degradation. In the present work, the degrading potential of Y. lipolytica IMUFRJ 50682, isolated from tropical estuarine water in Rio de Janeiro (Brazil), was evaluated. It has been demonstrated that this strain is capable to assimilate hydrocarbons, growing in medium containing crude oil as unique carbon source. To investigate which groups of compounds present in crude oil this strain is able to degrade, Y. lipolytica IMUFRJ 50682 was grown in medium containing petroleum Marlin (19API) at 28◦ C and 160 rpm for five days. The residual petroleum was submitted to gas chromatographic—mass spectrometry analysis (GC—MS) and the obtained chromatographic firgerprints were compared with the control assay residual petroleum that was also incubated in the same conditions. Y. lipolytica appears to assimilate hydrocarbons of high molecular weight such n-alkanes (C11 —C19 ), isoprenoids (pristane and phytane), aromatics with two or three aromatics rings (naphthalene, methylnaphthalenes, dimethylnaphthalenes, trimethylnaphthalenes, phenanthrene, methylphenanthrenes, dimethylphenanthrenes). In conditions of absence of these hydrocarbons, Y. lipolytica was also capable to consume more complex hydrocarbons as tricyclic terpanes. With that, Y. lipolytica IMUFRJ 50682 showed to be a potential crude oil degrading yeast. So, this strain can be used for bioremediation processes. doi:10.1016/j.nbt.2009.06.339
2.1.093 Isolation and characterization of halophilic bacteria from C ¸ amaltı Saltern Turkey M.B. Mutlu ∗ , K. Guven Anadolu University, Eskisehir, Turkey
C ¸ amaltı Saltern is the biggest coastal solar saltern on the Aegean cost of Turkey and produces most of the salt consumed in the country. The brines originate by evaporation of seawater (so-called thalassohaline brines) and reflect the ionic composition of the sea. This study was carried out to evaluate the microbial diversity of C ¸ amaltı Saltern, since there are no detailed reports on microbiata of this hypersaline environment. The aim of this study were isolate halophilic bacteria from the saltern and identify the isolates by their 16S rRNA sequences. Samples were taken from different pans of C ¸ amaltı Saltern in July 2006. Isolation was carried by using 25% Sea Water Agar. Identification of the isolates have been performed by using 16S rRNA sequence analysis. A total of 11 isolates from the saltern were chosen for the further tests. It has been found that all the isolates were belong to Bacteria domain. Identification of the isolates have been performed by using 16S rRNA sequence analysis. Isolates were identified as Halomonas, Halobacillus and Pontibacillus genus.
ABSTRACTS
2.1.094 To study characteristics of antibacterial of seven medicinal plants on two bacteria: Bacillus cereus and Salmonella A. Ganbari ∗ , N. Mahdi Nezhad, A. Heidari Department of Plant Breeding and Biocentre, Faculty of Agriculture, Zabol University, Zabol, Islamic Republic of Iran
Manufacturing of medicinal chemistry in spectra spread, from medicine in color, figs and different measurements appears by specialists. Manufacturing of this medicine caused to make Research on medicinal plant at once become to stagnancy. Because Physician without any Information of any event of medicinal chemistry and with anxiousness given to the patients. Until, Gradually was talking about Identicalness, problem of effects of body direction, but also about medicine was heard on scientific society with this regards, at end specialists was of this experiences of scientific, Gradually they come to know about disadvantages of use of medicine with effects of materials. specialist twenty century Named as extract of twenty century of medicinal plant. In this research with aim to investigate characteristics of antibacterial extract of seven medicinal plant (thymus vulgaris L, pimpinella anisum L, Trigonella foenum-graecum L, Eucalyptus globoulus , Allium sativum L, Achillea millefolium L, Matricaria chamomilla) on two bacteria one of them Salmonella negative gram bacteria and second of them Bacillus.Cereus Positive gram bacteria. Experiment was conducted in molecular biology center in Zabol University during 2007. Experiment has been design four replication by two culture media of PDA and Nutrient Agar by two kind of bacteria in Petri dishes and mentioned 5% extract of medicinal plant by standard Disc diffusion (6.4 mm) in sterile complete conditions in 37◦ C during 24 hours. With this regards, results has been shown and observed the bacteria Gram positive Bacillus.Cereus related to bacteria Gram negative Salmonella showed more sensitivity, but not significant differences. Diameter of media, non-existence of bacteria with attention of treatment was good and this, indication from characteristics of antibacterial of mentioned medicinal plant specially of Trigonella foenumgraecum L. Experiment used according to statistical, analysis of variance, diameter of media, non-existence of extract of medicinal plant, showed to high significant difference and characteristics of antibacterial of extract of seven medicinal plant such as. Trigonella foenum-graecum L. having maximum effects as Thymus vulgaris having minimum effects. doi:10.1016/j.nbt.2009.06.341
doi:10.1016/j.nbt.2009.06.340
www.elsevier.com/locate/nbt S81
of energy and carbon Yarrowia lipolytica is an unique strictly aerobic yeast with the ability to degrade very efficiently hydrophobic substrates such as n-alkanes, fatty acids, fats and oils for which it has specific metabolic pathways. So, Y. lipolytica is emerging as a promising agent in crude oil degradation. In the present work, the degrading potential of Y. lipolytica IMUFRJ 50682, isolated from tropical estuarine water in Rio de Janeiro (Brazil), was evaluated. It has been demonstrated that this strain is capable to assimilate hydrocarbons, growing in medium containing crude oil as unique carbon source. To investigate which groups of compounds present in crude oil this strain is able to degrade, Y. lipolytica IMUFRJ 50682 was grown in medium containing petroleum Marlin (19API) at 28◦ C and 160 rpm for five days. The residual petroleum was submitted to gas chromatographic—mass spectrometry analysis (GC—MS) and the obtained chromatographic firgerprints were compared with the control assay residual petroleum that was also incubated in the same conditions. Y. lipolytica appears to assimilate hydrocarbons of high molecular weight such n-alkanes (C11 —C19 ), isoprenoids (pristane and phytane), aromatics with two or three aromatics rings (naphthalene, methylnaphthalenes, dimethylnaphthalenes, trimethylnaphthalenes, phenanthrene, methylphenanthrenes, dimethylphenanthrenes). In conditions of absence of these hydrocarbons, Y. lipolytica was also capable to consume more complex hydrocarbons as tricyclic terpanes. With that, Y. lipolytica IMUFRJ 50682 showed to be a potential crude oil degrading yeast. So, this strain can be used for bioremediation processes. doi:10.1016/j.nbt.2009.06.339
2.1.093 Isolation and characterization of halophilic bacteria from C ¸ amaltı Saltern Turkey M.B. Mutlu ∗ , K. Guven Anadolu University, Eskisehir, Turkey
C ¸ amaltı Saltern is the biggest coastal solar saltern on the Aegean cost of Turkey and produces most of the salt consumed in the country. The brines originate by evaporation of seawater (so-called thalassohaline brines) and reflect the ionic composition of the sea. This study was carried out to evaluate the microbial diversity of C ¸ amaltı Saltern, since there are no detailed reports on microbiata of this hypersaline environment. The aim of this study were isolate halophilic bacteria from the saltern and identify the isolates by their 16S rRNA sequences. Samples were taken from different pans of C ¸ amaltı Saltern in July 2006. Isolation was carried by using 25% Sea Water Agar. Identification of the isolates have been performed by using 16S rRNA sequence analysis. A total of 11 isolates from the saltern were chosen for the further tests. It has been found that all the isolates were belong to Bacteria domain. Identification of the isolates have been performed by using 16S rRNA sequence analysis. Isolates were identified as Halomonas, Halobacillus and Pontibacillus genus.
ABSTRACTS
2.1.094 To study characteristics of antibacterial of seven medicinal plants on two bacteria: Bacillus cereus and Salmonella A. Ganbari ∗ , N. Mahdi Nezhad, A. Heidari Department of Plant Breeding and Biocentre, Faculty of Agriculture, Zabol University, Zabol, Islamic Republic of Iran
Manufacturing of medicinal chemistry in spectra spread, from medicine in color, figs and different measurements appears by specialists. Manufacturing of this medicine caused to make Research on medicinal plant at once become to stagnancy. Because Physician without any Information of any event of medicinal chemistry and with anxiousness given to the patients. Until, Gradually was talking about Identicalness, problem of effects of body direction, but also about medicine was heard on scientific society with this regards, at end specialists was of this experiences of scientific, Gradually they come to know about disadvantages of use of medicine with effects of materials. specialist twenty century Named as extract of twenty century of medicinal plant. In this research with aim to investigate characteristics of antibacterial extract of seven medicinal plant (thymus vulgaris L, pimpinella anisum L, Trigonella foenum-graecum L, Eucalyptus globoulus , Allium sativum L, Achillea millefolium L, Matricaria chamomilla) on two bacteria one of them Salmonella negative gram bacteria and second of them Bacillus.Cereus Positive gram bacteria. Experiment was conducted in molecular biology center in Zabol University during 2007. Experiment has been design four replication by two culture media of PDA and Nutrient Agar by two kind of bacteria in Petri dishes and mentioned 5% extract of medicinal plant by standard Disc diffusion (6.4 mm) in sterile complete conditions in 37◦ C during 24 hours. With this regards, results has been shown and observed the bacteria Gram positive Bacillus.Cereus related to bacteria Gram negative Salmonella showed more sensitivity, but not significant differences. Diameter of media, non-existence of bacteria with attention of treatment was good and this, indication from characteristics of antibacterial of mentioned medicinal plant specially of Trigonella foenumgraecum L. Experiment used according to statistical, analysis of variance, diameter of media, non-existence of extract of medicinal plant, showed to high significant difference and characteristics of antibacterial of extract of seven medicinal plant such as. Trigonella foenum-graecum L. having maximum effects as Thymus vulgaris having minimum effects. doi:10.1016/j.nbt.2009.06.341
doi:10.1016/j.nbt.2009.06.340
www.elsevier.com/locate/nbt S81