Isolation and complete sequence of the pur L gene encoding FGAM synthase II in Lactobacillus casei

Gene, 133 (1993) 147 0 1993 Elsevier Science Publishers

GENE

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Corrigendum Gene, 119(1992) 123-126 0 1992 Elsevier Science Publishers

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Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei (Purine operon; pa&; purQ; purF)

Zheng-Ming GL+* Duane W. Martindale” and Byong H. Leeb “Department of Microbiology, and bDepartment of Food Science and Agricultural Chemistry, McGill University, Macdonald Campus, Ste. Anne de Bellevue, Quebec, H9X 3V9, Canada. Tel. (514) 398-7979

We have discovered several sequencing errors over one area (nt 2360-2395) of the sequence included in the above paper. The correct sequence should have two additional adenines between nt 2361 and 2362, the adenine at nt 2370 should be deleted, and one thymine should be added between nt 2385 and 2386, and between nt 2394 and 2395. The start codons (ATG) and nt present in one sequence, but not in the other are underlined below. Fig. 2, lines starting with 2281, should be corrected as follows, starting with nt 2346:

Asterisks mark the stop codons. Note that the net effect of the changes is an addition of three nt so that the reading frame is only affected over this region. The putative purL product is shorter by one aa. The N-terminal end of the putative product of the corrected L. casei (Lc) purF gene now matches that of B. subtilis (Bs) to a much

Correspondence

to: Dr. D. Martindale,

Department

of Microbiology,

21,111 Lakeshore Rd., Ste. Anne de Bellevue, Quebec, H9X lC0, Canada. Tel. (5 14) 398-7886; Fax (l-514) 398-7624. * Present address: Institute of Parasitology, McGill University, Macdonald Campus, 21,111 Lakeshore Rd., Ste. Anne de Bellevue, Quebec H9X ICO, Canada. Tel. (I-514) 398-7999.

greater extent. Two lines of Fig. 4 should be corrected as follows:

(5 ’ end) .. Original: 1 MPHEPRLKMKNAGFRCWG Lc

gurF

.

Bs

I

:I

II

1 MLAEIKGLNEECGVFGIWG

Corrected: 1 MPHEPKGLNEECGVFGVWG Lc I I IIIIIIIIIII:II 1 MLAEIKGLNEECGVFGIWG Bs We thank Dr. Howard Zalkin for pointing out that our sequence was probably incorrect in this region based upon their studies of the N-terminal end of amidophosphoribosyltransferase (the purF product) from several organisms [Zhou, G., Broyles, S.S., Dixon, J.E. and Zalkin, H.: Avian glutamine phoshoribosylpyrophosphate amidotransferase propeptide processing and activity are dependent upon essential cysteine residues. J. Biol. Chem. 267 (1992) 7936-79421.

Abbreviations: aa, amino acid(s); L., Lactobacillus; nt, nucleotide(s); purF, gene encoding amidophosphoribosyltransferase; purL, gene encoding phosphoribosylformylglycinamidine (FGAM) synthase II.