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Isolation and identification of alkaloids of Erythrina abyssinica A Sudanese medicinal plant
S40
Abstracts
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9 ±1.5 vs. 297±2.5 nmol/g tissue for TQ, 10 mg/kg; p<0.001 and 153.5±1.3 nmol/g tissue for NSO, 0.384 mg/kg; p<0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus. Keywords: Nigella sativa, Thymoquinone, Lipid peroxidation, Ischemia–reperfusion injury (IRI), Hippocampus doi:10.1016/j.clinbiochem.2011.08.1051
Oral – [A-10-658-6] Effect of silibinin on telomerase gene expression in breast cancer cell line (T47D) Marzieh Nasiri, Nosratollah Zarghami, Mohammad Pourhassan Moghaddam, Mohammad Rahmati, Kazem Nejati-Koshki, Mahdieh Mollazadeh, Raana Jahanban, Amin Barkhordari Clinical Biochemistry and Laboratory Medicine, Tabriz University Of Medical Sciences, Tabriz, Iran E-mail address: [email protected] (N. Zarghami) Current therapies for breast cancer include treatments which are toxic and show often drug resistance. Telomerase, a cellular reverse transcriptase that maintains the ends of linear chromosomes (telomeres), is activated in the vast majority of breast cancers but not in normal adjacent tissues. Therefore its target, preferably with natural compounds including herbal extracts such as silibinin, is desirable. Silibinin, a major biologically active portion of the plant extract from milk thistle (Silybum marianum), has anti-cancer properties. Therefore, the main objective of current work was to study the inhibitory effect of silibinin on telomerase gene expression in T47D breast cancer cell line after MTT assay for 24, 48 and 72 h. Finally, after determination of IC50, cells were treated with silibinin and real time PCR was done to measure the amount of telomerase inhibition by the silibinin. Data analysis showed that silibinin has time and dose-dependent cytotoxic effect on T47D breast cancer cell line with IC50 = 109, 75, 31 μM for 24, 48, 72 h, respectively and this compound inhibits telomerase gene expression with time and dose-dependent manner. Analysis of data showed significant decrease in telomerase expression with increase in silibinin concentration (p-value< 0.05). In conclusion, silibinin has cytotoxic and telomerase inhibitory effect on cell line T47D and it may be exploited as potential source for developing novel drugs against breast cancer. Keywords: Silibinin, Telomerase, Breast cancer doi:10.1016/j.clinbiochem.2011.08.1052
Oral – [A-10-672-1] Isolation and identification of alkaloids of Erythrina abyssinica A Sudanese medicinal plant Ahmsd Gagdima, Adam Musab a Peace University, Sudan b Peace University, Sudan, Giri-adam E-mail address: [email protected] (A. Gagdim)
Erythrina abyssinica Lam.; leguminosae family (Fabaceae) is widespread from Sudan and Ethiopia south to Angola, Zimbabwe and Mozambique. It has been introduced as an ornamental in Mauritius and various places in tropical Asia and Central America. Erythrina abyssinica is well known as a medicinal plant. The bark is most commonly used in traditional medicine, to treat snakebites, malaria, sexually transmittable diseases such as syphilis and gonorrhoea, amoebiasis, cough, liver inflammation, stomach-ache, colic and measles. Roasted and powdered bark is applied to burns, ulcers and swellings. The liquid from crushed bark of green stems is used to cure conjunctivitis caused by Chlamydia trachomatis (trachoma), whereas bark sap is also drunk as an anthelmintic. The bark is also applied against vomiting. Pounded flowers serve to treat dysentery. A maceration of the flower is drunk as an abortifacient, and applied externally to treat earache. Roots are taken to treat peptic ulcers, epilepsy, malaria, blennorrhagia and schistosomiasis. Leaves are taken to treat peptic ulcers; they are also used for treatment of diarrhoea. A leaf decoction serves as an emetic. Leaves are applied externally to wounds and painful joints; they are also applied to treat skin diseases in cattle. Fruit extracts are taken to treat asthma and meningitis. The alkaloid constituents of the stem bark, and leaves, of Erythrina abyssinica Lam.; leguminosae family (Fabaceae) growing in Sudan were isolated and identified. The crude alkaloidal content was isolated according to the `acid-base shakeout` method, after then, it's cytotoxicity was in vitro evaluated against HeLa, Hep-G2, HEP-2, HCT116, MCF-7 and HFB4, and resulted with IC50= 13.8, 10.1, 8.16, 13.9, 11.4 and 12.2 μg/mL, respectively. Further chromatographic fractionation of the crude alkaloidal fraction led to the isolation of Erythraline (1), Erysodine (2), Erysotrine (3), 8-Oxoerythraline (4) and 11-Methoxyerysodine (5), it is the first time for compound (IV and amp;V) to occur in this species. The isolated alkaloids showed in vitro cytotoxic activity against Hep-G2 with IC50= 17.6,11.8,15.8, 3.89 and 11.4 μg/mL respectively, and against HEP-2 with IC50 =15.90, 19.90, 21.60, 18.50 and 11.50 μg/mL respectively. Furthermore, the alkaloidal fraction caused a reduction for the viability of mock-infected MT-4 cells by CC50 =53 μM and achieve 50% protection of MT-4 cells from the HIV-1 induced cytopathogeneticy by EC50 = >53 μM, as compared with EFV as positive control which showed CC50= 45 μM and EC50 =0.003 μM, as determined by the MTT method. Keywords: Erythrina abyssinica Lam., Fabaceae (Leguminosae), Alkaloids, Anti-HIV-1, Cytotoxicity doi:10.1016/j.clinbiochem.2011.08.1053
Oral – [A-10-795-1] Screening the methanol extracts of some Iranian plants for tyrosinase inhibitory activity Ahmad Gholamhoseinian, Zohreh Razmi Kerman University of Medical Sciences, Iran E-mail addresses: [email protected] (A. Gholamhoseinian), [email protected] (Z. Razmi) Introduction: Melanin is responsible for pigmentation of skin and hair. It protects our skin from the harmful effects of UV radiation. However abnormal production of it may lead to different diseases such as hyper-pigmentation or vitiligo. Tyrosinase is a key enzyme in melanin production therefore inhibitors of this enzyme should have broad application. Due to the adverse side effects of synthetic inhibitors that are currently being used, the search for new inhibitors specially from natural sources is needed. Materials and method: The methanol extracts of 80 Iranian plants were prepared. The inhibitory effects of these extracts on tyrosinase activity were determined by spectrophotometric methods at 492 nm. The inhibition mode of effective plant extracts was determined using the Lineweaver–Burk plots.
Abstracts
The transient global cerebral ischemia was induced using four-vesselocclusion method for 20 min. Lipid peroxidation level in hippocampus portion was measured as malondialdehyde (MDA) based on its reaction with thiobarbituric acid (TBA) following ischemic insult. Results: The transient global cerebral ischemia induced a significant increase in TBA reactive substances (TBARS) level (p < 0.001), in comparison to sham-operated animal. Pretreatment with TQ and NSO resulted to a significant decrease in MDA level as compared to ischemic group (66.9 ±1.5 vs. 297±2.5 nmol/g tissue for TQ, 10 mg/kg; p<0.001 and 153.5±1.3 nmol/g tissue for NSO, 0.384 mg/kg; p<0.001). Using a reversed-phase HPLC system, the amount of TQ in NSO was 0.58% w/w. Conclusion: These results suggested that TQ and NSO may have protective effects on lipid peroxidation process during IRI in rat hippocampus. Keywords: Nigella sativa, Thymoquinone, Lipid peroxidation, Ischemia–reperfusion injury (IRI), Hippocampus doi:10.1016/j.clinbiochem.2011.08.1051
Oral – [A-10-658-6] Effect of silibinin on telomerase gene expression in breast cancer cell line (T47D) Marzieh Nasiri, Nosratollah Zarghami, Mohammad Pourhassan Moghaddam, Mohammad Rahmati, Kazem Nejati-Koshki, Mahdieh Mollazadeh, Raana Jahanban, Amin Barkhordari Clinical Biochemistry and Laboratory Medicine, Tabriz University Of Medical Sciences, Tabriz, Iran E-mail address: [email protected] (N. Zarghami) Current therapies for breast cancer include treatments which are toxic and show often drug resistance. Telomerase, a cellular reverse transcriptase that maintains the ends of linear chromosomes (telomeres), is activated in the vast majority of breast cancers but not in normal adjacent tissues. Therefore its target, preferably with natural compounds including herbal extracts such as silibinin, is desirable. Silibinin, a major biologically active portion of the plant extract from milk thistle (Silybum marianum), has anti-cancer properties. Therefore, the main objective of current work was to study the inhibitory effect of silibinin on telomerase gene expression in T47D breast cancer cell line after MTT assay for 24, 48 and 72 h. Finally, after determination of IC50, cells were treated with silibinin and real time PCR was done to measure the amount of telomerase inhibition by the silibinin. Data analysis showed that silibinin has time and dose-dependent cytotoxic effect on T47D breast cancer cell line with IC50 = 109, 75, 31 μM for 24, 48, 72 h, respectively and this compound inhibits telomerase gene expression with time and dose-dependent manner. Analysis of data showed significant decrease in telomerase expression with increase in silibinin concentration (p-value< 0.05). In conclusion, silibinin has cytotoxic and telomerase inhibitory effect on cell line T47D and it may be exploited as potential source for developing novel drugs against breast cancer. Keywords: Silibinin, Telomerase, Breast cancer doi:10.1016/j.clinbiochem.2011.08.1052
Oral – [A-10-672-1] Isolation and identification of alkaloids of Erythrina abyssinica A Sudanese medicinal plant Ahmsd Gagdima, Adam Musab a Peace University, Sudan b Peace University, Sudan, Giri-adam E-mail address: [email protected] (A. Gagdim)
Erythrina abyssinica Lam.; leguminosae family (Fabaceae) is widespread from Sudan and Ethiopia south to Angola, Zimbabwe and Mozambique. It has been introduced as an ornamental in Mauritius and various places in tropical Asia and Central America. Erythrina abyssinica is well known as a medicinal plant. The bark is most commonly used in traditional medicine, to treat snakebites, malaria, sexually transmittable diseases such as syphilis and gonorrhoea, amoebiasis, cough, liver inflammation, stomach-ache, colic and measles. Roasted and powdered bark is applied to burns, ulcers and swellings. The liquid from crushed bark of green stems is used to cure conjunctivitis caused by Chlamydia trachomatis (trachoma), whereas bark sap is also drunk as an anthelmintic. The bark is also applied against vomiting. Pounded flowers serve to treat dysentery. A maceration of the flower is drunk as an abortifacient, and applied externally to treat earache. Roots are taken to treat peptic ulcers, epilepsy, malaria, blennorrhagia and schistosomiasis. Leaves are taken to treat peptic ulcers; they are also used for treatment of diarrhoea. A leaf decoction serves as an emetic. Leaves are applied externally to wounds and painful joints; they are also applied to treat skin diseases in cattle. Fruit extracts are taken to treat asthma and meningitis. The alkaloid constituents of the stem bark, and leaves, of Erythrina abyssinica Lam.; leguminosae family (Fabaceae) growing in Sudan were isolated and identified. The crude alkaloidal content was isolated according to the `acid-base shakeout` method, after then, it's cytotoxicity was in vitro evaluated against HeLa, Hep-G2, HEP-2, HCT116, MCF-7 and HFB4, and resulted with IC50= 13.8, 10.1, 8.16, 13.9, 11.4 and 12.2 μg/mL, respectively. Further chromatographic fractionation of the crude alkaloidal fraction led to the isolation of Erythraline (1), Erysodine (2), Erysotrine (3), 8-Oxoerythraline (4) and 11-Methoxyerysodine (5), it is the first time for compound (IV and amp;V) to occur in this species. The isolated alkaloids showed in vitro cytotoxic activity against Hep-G2 with IC50= 17.6,11.8,15.8, 3.89 and 11.4 μg/mL respectively, and against HEP-2 with IC50 =15.90, 19.90, 21.60, 18.50 and 11.50 μg/mL respectively. Furthermore, the alkaloidal fraction caused a reduction for the viability of mock-infected MT-4 cells by CC50 =53 μM and achieve 50% protection of MT-4 cells from the HIV-1 induced cytopathogeneticy by EC50 = >53 μM, as compared with EFV as positive control which showed CC50= 45 μM and EC50 =0.003 μM, as determined by the MTT method. Keywords: Erythrina abyssinica Lam., Fabaceae (Leguminosae), Alkaloids, Anti-HIV-1, Cytotoxicity doi:10.1016/j.clinbiochem.2011.08.1053
Oral – [A-10-795-1] Screening the methanol extracts of some Iranian plants for tyrosinase inhibitory activity Ahmad Gholamhoseinian, Zohreh Razmi Kerman University of Medical Sciences, Iran E-mail addresses: [email protected] (A. Gholamhoseinian), [email protected] (Z. Razmi) Introduction: Melanin is responsible for pigmentation of skin and hair. It protects our skin from the harmful effects of UV radiation. However abnormal production of it may lead to different diseases such as hyper-pigmentation or vitiligo. Tyrosinase is a key enzyme in melanin production therefore inhibitors of this enzyme should have broad application. Due to the adverse side effects of synthetic inhibitors that are currently being used, the search for new inhibitors specially from natural sources is needed. Materials and method: The methanol extracts of 80 Iranian plants were prepared. The inhibitory effects of these extracts on tyrosinase activity were determined by spectrophotometric methods at 492 nm. The inhibition mode of effective plant extracts was determined using the Lineweaver–Burk plots.