Isolation and “in vitro” maintenance of plasmodium falciparum

Poster Sessions / Parasitology

International

P-0648

P-0650

OF tlVE DIFFERENT MEDIA FOR ENTAMOEBA HIBTOLYTICA .

EVALUATION CULllVATlO?d WA’, Depilnd

shuty

wu

made

inordcrtodctumhemit?.bkx&

foridiatkmdrmrintenraccofE.tim Wehave been abk to imbtctwostcainsofE.hbdytidE.diq=firom Imlial canim anfi wckmmrintriaedthcmiffdllowing me& mciuccOctober 1996 : l- TYSGM-9 2- HSr+s 3Robhum ,4-LiverhfiuihAep,5-RPMhw.Thcatnoeba ware sthdund thm tim&perwcckandthcmubiphhn me&

mteoflzaumoc~waocalc~forlwomontha.our~ indiceumiihemosisuitdhmcdiaforxcnic-of E.w

are

Rob&m

:

RF’MI1640 and HSr+s

rcapoh+.Tothtbcstofmy~mC

GROWTH OF ACANTHAMOEBA SP. STRAINS ON BACTERLA AND FUNGI ISOLATED FROM CONTACT LENS STORAGE CASES

and Immunology,

Acanthamoeba is a free-living protozoan and well established causative agent of keratitis, particularly in contact lens (CL) wearers. The most usual source of amebas is CL storage cases and solutions previously contaminated by bacteria/fungi that parasite selectively use as food source. We investigated the role of these contaminants as substrates for the growth of environmental and human strain of Acanthamoebo sp. isolated from comeal ulcer. The strains were maintained as axenic cultures in serumcasein-glucose-yeast extract medium. For preparing inoculum 72h old axenic culture was used and final concentration of amehas in saline was l.5x105/ml and bacteria and fungi 0.5 MC Farland units. Cultures were incubated at 30°C and number of trophozoites was counted in triples after 24h in hemocytometer. As control Enterobacter cloacae WBSused as one of official substrates for the isolation of amebas from clinical specimens. Serratialiqueficiens and Alcaligenes faecalis caused the highest grade of growth, even higher than E. cloacne. In the presence of Staphylococcus aureus, S. epidermidis, diphtheroids, Mormella sp. and Candida albicans there was no significant difference in growth than with E. cloacae. For the clinical strain Moraxella sp. appeared to be a better growth-promoting

of Pfn&&y&MycQlogy,Sclwo10fMedicine, Behe&tyuniver+ofh4ed.Scknas,Irsn.

A

297

DZamiC MA, KranjEiC-Zec I, MitroviC S, ArsiC V Department of Parasitology, Institute of microbiology University of Belgrade School of Medicine, Yugoslavia

XENIC

OF

RFZAEIANW.

Shabeed

47 (Suppl.) (1998) 283-389

cdturcof

E.hitd#atolheRPh4I1uoMnotbanachiedhithefto.

bacterial

substrate

than

for

environmental

strain.

At

the

contrary,

the

Pseudomonas aeruginoso caused certain reduction in the number of trophozoites and appearance of cystic forms of both strains. Our findings suggest that contamination of CL storage cases with parasite and especially Gram negatives such as S. lique/aciens and A. ,fiecalis presence

of

capable. of supporting pathogenesis

amebas.

The

microfolora

amebic

presence could

of

P-0651

ZYMODEME ISOLATED Kdar

S.

PATTERNS

OF ENTAMOEBA

FROM CLINICAL

Bahar iH.

HISTOLYTICA

STRAINS

he an important

noted

bacteria

on

of importance

with

this agent.

The

in the

as transient of protozoa,

same bacteria

of amebas than

could

in be

E. cloacae

specimens.

ISOIATION AND

Pardruc 8. ‘tie

factor

conjunctiva

in existence

used as better substrate for isolation

from the clinical

P-0649

could

keratitis by the provision of large inocula of

be a factor

case of eye contamination eventually

growth

of Acanfhomoeba

“in v/iWkWNlENMCE Plewnodium fe&@dntm,

c:,

Cobezrs c

l

OF

’

PATIENTS

* Pwooltology Dhdoion. Nollonol Cm\er of Loborotories of Pubtc Hooth, INS, Lbnr, Ponl. +. Gonel Director of Notlonoi Corder PI Lobo~Mofies of PII& Hooth. INS, Lii.

Yulug N

Dokuz Evliil University, Faculty of Medicine, Department of Microbiology.

itidu. Lmir,TUiye Throughout

the world,

Mobrio by Pkwncdium f&i$wurn is and importud pmbcm in many areas. mainty aml~on b&n of Perk amebiasis

is a common

medical

problem

both

developed and developing countries While 10% of infected patients invasive amebiasis, the reman@ asymptomatic. Entamoeba histolyttca

in

suffer

IS now known to consist of hvo gene&ally distinct species, the invasive parasite E. histolytico, which is the etiologic agent of amebic colitis and liver abscess, and the noninvasive parasite Entamorba Lspar. Micr~~~~py cannot diiemntiate E. hrsfolytico from E. dispar. culture is more senative than microscopy, and woenzyme analysis of the cultured amoebae enables differentiation of from E. dwpar. Snubs on iwenzyme patterns (zymodems) of E.hrstolytlco isolated from thousands of asymptomatic and symptomatic human cases have resulted in the characterization of at least 23 distinct zymodems. Based on the clinical picture of the source of the isolate. these rymodemes are placed m one of two categotiexpathogenic or nonpathogenic. Two isoeruyme systems, the hexokinase (HK) and phosfoglucomutase (PC&Q, have been found to differ m their electrofomtic migration m pathogenic and nonpathogenic E histolyka The nonpathogenic isoenzyme patterns. with rare exception,are ohtamed m tsolates from asymptomatic carriers with negative serology,while the pathogenic zymodemes. which are the minority of cases. are usually derived from individuals wth positive serology and presence of some clinical symptoms. Among the studied isolates. malic enzyme produced charecteristically a showed double bands. phosphoglucomutase and smgle band. hexokinase glucosephosphate iwmerase showed a rmgk band. hy references pmperl~. were detected

f%btc Heath

Tho ctnkol reolotonce of lnMabrlaldrugs Euch os CMroqub~r and our ignomnco on the P. Fdciprrum stnin cir~ulatii mnong the dck pwpb &l&cd. us to intold the loolotion and mointononco of P. Fo/c&wm Mder cutural condilions to chomcterize il. Blood somplos of 10 msbrial cooes by P. foki~rum vddch diognosb woo mode by thick Rhr, bwo cutured h RFM-lg40 (SlCh44) mdlum khwing Trogor and Jonnon method. The medium wof suppkmerdod 4th Hepec, Gontnnyein, 10% inootiwtod human pbsmo (9’ group), 5% of hcmotocryle (Humon Red Ceto, 73’ group) ond incubolod 1 37%. Tho cutum modhun wet changed every day and Ibo pororilos worn ovoklod by Giomso steined kts. The resuts showed pwooitc roprodulion in o period of 6 to 31 doyo. AUor of t0 days of mrinlononco. Ihe panoiled Rod cots ohwdg good lmounl of fint forms were cryopnrowod under otondofd procedunl. From the 10 mat&al studies cases, the cuture r9t ebt? in eigth. Thoro parasites nit be goneticaty chrrrderizod md its -in vitro. rosislsnce to drugs sludiid.