- Email: [email protected]
Isolation virulent strain of the Trypanosoma equiperdum for preparation of trypanosoma antigen
S70
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
Correlation between renal resistive index and histological changes in dogs with chronic kidney disease Radu Constantinescu 1,∗ , Mario Darius Codreanu 1 , Iuliana Codreanu 2 1 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania 2 Department of Physiology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania
E-mail address: [email protected] (R. Constantinescu). The resistive index (RI) represent an indirect measurement that may provide information over changes in the renal blood flow resistance. RI was calculated from the duplex Doppler waveform, according to the Pourcelot’s formula, from the arcuate arteries, in one kidney, with 10 min before performing renal biopsy in 19 dogs diagnosed with chronic kidney disease (CKD). The RI values were compared with laboratory findings and the results of renal biopsy. The goal of this paper is to appreciate the association between RI and pathological lesions. The present study was carried out in the Internal Medicine Department of the Faculty of Veterinary Medicine, Bucharest, over a period of 26 months, from September 2013 to March 2016. RI was not different whether hyaline deposits were present or not (0.65 vs 0.64) or with normal intima or moderate intima thickening. Patients with severe arteriosclerosis had a significantly higher RI than those with no or moderate arteriosclerosis (0.73 vs 0.61). No association was found between RI value and glomerulosclerosis. Patients with isolated glomerular involvement had significant lower RI than those with interstitial fibrosis and/or vascular lesions (0.62 vs 0.74). The results of this study indicates that RI values are slightly increased in some forms of renal medical disease but not in others and also could give the opportunity to identify CKD patients at high risk of end stage renal disease. http://dx.doi.org/10.1016/j.jbiotec.2016.05.255 MYC inhibition may be a promising strategy for a significant fraction of SCLC Onur Tokgun ∗ , Pervin Elvan Tokgun, Hakan Akca, Gulseren Bagci Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey E-mail address: [email protected] (O. Tokgun). Small cell lung cancer (SCLC) is the most aggressive type of lung cancer with high mortality. One of the MYC family genes, MYC, MYCL1 or MYCN, is amplified and overexpressed in ∼20% of SCLC; therefore, we have selected these MYC family genes as strong candidates of targets for therapy. Our goal is to investigate the biological significance of MYC family gene amplification for SCLC cells by either down-regulation of MYC family genes or suppression of their activities with inducible shRNAs and siRNAs To check the effect of Myc expression on cellular proliferation in SCLC cell lines, we constructed constitutive and inducible Myc expression vectors and inducible lentiviral vector for MYC inhibition strategy. The effect of Myc expression on cellular
proliferation and gene expression profiling were evaluated in Myc overexpressed and knockdown SCLC cells. Constitutive and inducible expression of Myc induced cellular proliferation in Myc non-amplified SCLC cells. Importantly, c-Myc downregulation markedly reduced the enhanced proliferation with ectopic expression of c-myc. And in MYC overexpressed cells we evaluated inhibitory effects of shRNAs on cellular proliferation. Due to changes in Myc expression, we evaluated the changes of some proliferation and apoptosis markers by western blot and qRT-PCR. The results indicate that SCLC cells are addicted to Myc proteins for their growth. Therefore, they are highly sensitive to MYC inhibition. http://dx.doi.org/10.1016/j.jbiotec.2016.05.256 Isolation virulent strain of the Trypanosoma equiperdum for preparation of trypanosoma antigen Gulnazi Daulethanovna Akhmetova 1 , Shabdarbaeva Gulnar Sabirovna 2 , Turganbaeva Gulnar Eldesbaevna 3 , Usmangalieva Symbat Suttybaevna 1 , Nurgazy Banu Omirtaevna 4 , Khussainov Damir Mikhdatovich 2 , Abeuov Khairulla Bilalovich 5 , Mukhitdinova Gulnar Yergalievna 6 , Assylkhanov Darkhan Ualievich 3,∗ 1
Department of Biological Safety, Kazakh National Agrarian University, Almaty, Kazakhstan 2 Scientific and Production Enterprise “Antigen” LLP, Almaty region, Kazakhstan 3 Scientific and Production Enterprise “Antigen” LLP, Laboratory of Parasitology, Almaty region, Kazakhstan 4 Kazakh National Agrarian University, Department of Biological Safety, Almaty, Kazakhstan 5 Kazakh Veterinary Scientific-Research Institute JSC “KazAgroInnovation”, Almaty, Kazakhstan 6 Department of Clinical Veterinary Medicine, Almaty, Kazakhstan E-mail address: [email protected] (A.D. Ualievich).
Among the widespread protozoan diseases causing economic damage to development of horse breeding in Kazakhstan and the important disease is Dourine. For diagnosis of trypanosomosis in horses used to serologic diagnosis. For increase of trypanosomes in blood the great influence is exerted by virulence of parasite. Scientists confirmed that mass reproduction of trypanosomes depends on methods of infestation. Preparation of an antigen for diagnosis of trypanosomosis of horses on the basis of virulent strain of Trypanosoma equiperdum. In study we used the local strain of T. equiperdum isolated from sick horses. For isolation virulent strain of T. equiperdum infected 5 white mice with the isolated blood the infected rabbits (I – passage). Height of parasitemia in mice took blood were bled and injected it into the following party (II – passage) and carried out X passages. I passage of a trypanosome in blood of white mice appeared in single quantities (1–5 trypanosomes 1 f.o.v) for 4 days. Parasitic reaction increased till 20–65 1 f.o.v and killed mice for 21–24 days. Parasites of the II–VI – passages were found 3–4 days, their quantity has increased 90–95 1 f.o.v parasites. Mice killed for 6–7 days. VII–X passages significantly shorten the appearance of parasites in blood, for 3 days the parasitemia was 90-110 1 f.o.v trypanosomes. The death of mice was observed for 3–4 days.
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
The results demonstrated that the virulence of strain of T. equiperdum can be increased with VII – passage. http://dx.doi.org/10.1016/j.jbiotec.2016.05.257 Effects of size grading and size difference on growth performance of hybrid tilapia (Oreochromis niloticus ♀ X Oreochromis aureus♂) juvenile in fiber tank conditions Suat Dikel Aquaculture Department, Faculty of Fisheries, Cukurova University, Faculty of Fisheries, Adana, Turkey E-mail address: [email protected]. The effects of size grading and size differences between small and large individuals on growth performance of hybrid tilapia (Oreochromis niloticus ♀ X Oreochromis aureus♂) juvenile were investigated. The 0 age 1–4 g hybrid fry which have not completed the juvenile period were stocked as a 40 fry/m3 in 500 l fiber tanks placed in a greenhouse and cultured for 60 days. Trails comprises four groups, the first graded group (G1), consisting of only small individuals, and three mixed groups consisting 1 g and 2 g (G2), 1–3 g (G3) and 1–4 g (G4) juvenile respectively. At the end of the experimental feeding period, it was observed that small fry in G2 were reached the best growth performance among the other groups (P < 0.01). Feed conversion ratio of group G2 (1.39 ± 0.05) was lower than the other groups (P < 0.05). In conclusion, size grading and size difference were affected on growth performance of hybrid tilapia fry, especially when juvenile were stocked as 1–2 g stocking composition (like G2), as a result of well competition can be established and growth performance of trail positively affected that. http://dx.doi.org/10.1016/j.jbiotec.2016.05.258 Degradation of clofibric acid by a phenol resistant Pseudomonas aeruginosa strain Sabra Hemidouche 1,2,∗ , Lidia Favier 3 , Zahra Sadaoui 1 , Abdeltif Amrane 3 1
Laboratoire de Génie de la Réaction, Faculté de Génie Mécanique et de Génie des Procédés, Université des Sciences et de la Technologie Houari, Boumediene, BP n◦ 32 el Alia Bab -Ezzouar, 16111 Alger, Algeria 2 Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, BP 384, Siège ex-Pasna Zone Industrielle, Bou-Ismail, CP 42004 Tipaza, Algeria 3 Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Université Européenne de Bretagne, 11 Allée de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France E-mail address: [email protected] (S. Hemidouche).
This study provides an alternative solution for the bioremediation of clofibric acid (CLA) a human pharmaceutical, well known as refractory to the conventional wastewater treatment. The capacity of a phenol resistant Pseudomonas aeruginosa strain to remove CLA was investigated. Kinetic batch experiments were conducted in basal minimal medium under aerobic conditions and at different initial pollutant concentrations and in the presence or in the absence of a carbon co-substrate. For these tests the stirring
S71
rate, pH and temperature were fixed to 150 tr/min, 7.3 and 37 ◦ C, respectively. Samples were regularly taken over 7 days in order determine the optical density, pH and the pollutant concentration (by HPLC/UV). Results have shown that the bacterial metabolic activity is reflected by pH decreasing and consumptions of carbon co-substrates. It was observed that the CLA elimination yield ranges between 71% and 17%, depending on its initial concentration. http://dx.doi.org/10.1016/j.jbiotec.2016.05.259 Testing the killing ability of pig neutrophils after stimulation with an ethanolamine derivative Gabriel Gajaila ∗ , Iuliana Gajaila, Gabriel Cotor, Lucian Ionita Preclinical Sciences Department, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (G. Gajaila). The pig neutrophils are separated from blood samples by the use of density gradient with Percoll medium. The purified neutrophils are seeded into the walls of microtiter plates in a tissue culture medium supported with nutrients and buffering serum. The ethanolamine solution is added in the tissue culture and the tray is incubated at 37 ◦ C for 2 h in an optimum atmosphere. Viable staphylococci are used as phagocytosis target and reincubated with pig neutrophils such that an optimum number of these are taken into the cytoplasm of the phagocytic cells. After several incubation periods, the neutrophils can be removed from the culture well and lysed by exposure to water. The lysate was cultured in order to determine the number of viable staphylococci remaining at that time, relative to the total number of starting bacterial organisms. Viable staphylococci released from the cytoplasm may be cultured on agar plates and the number of colonies enumerated. Neutrophils will progressively kill an increasing numbers of staphylococci, so that there are fewer colonies at each time point of the experiment (30 min intervals over a two-hour period). The “killing curve” generated after the experiment allows the comparison of the phagocitary activity of normal and stimulated neutrophils. The results indicate a 30% decrease of the surviving staphylococci in the stimulated neutrophils vs. the unstimulated ones. http://dx.doi.org/10.1016/j.jbiotec.2016.05.260 Purification and characterization of a bioflocculant produced by Bacillus amyloliquefaciens AS21a Aynur Gul Karahan 1,∗ , Gizem Gunay 1 , Mehmet Lutfu Cakmakci 2 1
Suleyman Demirel University, Faculty of Engineering, Department of Food Engineering, Turkey 2 Ankara University, Faculty of Engineering, Department of Food Engineering, Turkey E-mail address: [email protected] (A.G. Karahan). In this study, 109 strains were isolated from samples of soil and waste water that were collected from I˙ zmir, Isparta, Antalya, Denizli, Balikesir and Bursa. Strains that will be determined the flocculating activities were investigated for their morphological
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
Correlation between renal resistive index and histological changes in dogs with chronic kidney disease Radu Constantinescu 1,∗ , Mario Darius Codreanu 1 , Iuliana Codreanu 2 1 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania 2 Department of Physiology, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania
E-mail address: [email protected] (R. Constantinescu). The resistive index (RI) represent an indirect measurement that may provide information over changes in the renal blood flow resistance. RI was calculated from the duplex Doppler waveform, according to the Pourcelot’s formula, from the arcuate arteries, in one kidney, with 10 min before performing renal biopsy in 19 dogs diagnosed with chronic kidney disease (CKD). The RI values were compared with laboratory findings and the results of renal biopsy. The goal of this paper is to appreciate the association between RI and pathological lesions. The present study was carried out in the Internal Medicine Department of the Faculty of Veterinary Medicine, Bucharest, over a period of 26 months, from September 2013 to March 2016. RI was not different whether hyaline deposits were present or not (0.65 vs 0.64) or with normal intima or moderate intima thickening. Patients with severe arteriosclerosis had a significantly higher RI than those with no or moderate arteriosclerosis (0.73 vs 0.61). No association was found between RI value and glomerulosclerosis. Patients with isolated glomerular involvement had significant lower RI than those with interstitial fibrosis and/or vascular lesions (0.62 vs 0.74). The results of this study indicates that RI values are slightly increased in some forms of renal medical disease but not in others and also could give the opportunity to identify CKD patients at high risk of end stage renal disease. http://dx.doi.org/10.1016/j.jbiotec.2016.05.255 MYC inhibition may be a promising strategy for a significant fraction of SCLC Onur Tokgun ∗ , Pervin Elvan Tokgun, Hakan Akca, Gulseren Bagci Department of Medical Biology, School of Medicine, Pamukkale University, Denizli, Turkey E-mail address: [email protected] (O. Tokgun). Small cell lung cancer (SCLC) is the most aggressive type of lung cancer with high mortality. One of the MYC family genes, MYC, MYCL1 or MYCN, is amplified and overexpressed in ∼20% of SCLC; therefore, we have selected these MYC family genes as strong candidates of targets for therapy. Our goal is to investigate the biological significance of MYC family gene amplification for SCLC cells by either down-regulation of MYC family genes or suppression of their activities with inducible shRNAs and siRNAs To check the effect of Myc expression on cellular proliferation in SCLC cell lines, we constructed constitutive and inducible Myc expression vectors and inducible lentiviral vector for MYC inhibition strategy. The effect of Myc expression on cellular
proliferation and gene expression profiling were evaluated in Myc overexpressed and knockdown SCLC cells. Constitutive and inducible expression of Myc induced cellular proliferation in Myc non-amplified SCLC cells. Importantly, c-Myc downregulation markedly reduced the enhanced proliferation with ectopic expression of c-myc. And in MYC overexpressed cells we evaluated inhibitory effects of shRNAs on cellular proliferation. Due to changes in Myc expression, we evaluated the changes of some proliferation and apoptosis markers by western blot and qRT-PCR. The results indicate that SCLC cells are addicted to Myc proteins for their growth. Therefore, they are highly sensitive to MYC inhibition. http://dx.doi.org/10.1016/j.jbiotec.2016.05.256 Isolation virulent strain of the Trypanosoma equiperdum for preparation of trypanosoma antigen Gulnazi Daulethanovna Akhmetova 1 , Shabdarbaeva Gulnar Sabirovna 2 , Turganbaeva Gulnar Eldesbaevna 3 , Usmangalieva Symbat Suttybaevna 1 , Nurgazy Banu Omirtaevna 4 , Khussainov Damir Mikhdatovich 2 , Abeuov Khairulla Bilalovich 5 , Mukhitdinova Gulnar Yergalievna 6 , Assylkhanov Darkhan Ualievich 3,∗ 1
Department of Biological Safety, Kazakh National Agrarian University, Almaty, Kazakhstan 2 Scientific and Production Enterprise “Antigen” LLP, Almaty region, Kazakhstan 3 Scientific and Production Enterprise “Antigen” LLP, Laboratory of Parasitology, Almaty region, Kazakhstan 4 Kazakh National Agrarian University, Department of Biological Safety, Almaty, Kazakhstan 5 Kazakh Veterinary Scientific-Research Institute JSC “KazAgroInnovation”, Almaty, Kazakhstan 6 Department of Clinical Veterinary Medicine, Almaty, Kazakhstan E-mail address: [email protected] (A.D. Ualievich).
Among the widespread protozoan diseases causing economic damage to development of horse breeding in Kazakhstan and the important disease is Dourine. For diagnosis of trypanosomosis in horses used to serologic diagnosis. For increase of trypanosomes in blood the great influence is exerted by virulence of parasite. Scientists confirmed that mass reproduction of trypanosomes depends on methods of infestation. Preparation of an antigen for diagnosis of trypanosomosis of horses on the basis of virulent strain of Trypanosoma equiperdum. In study we used the local strain of T. equiperdum isolated from sick horses. For isolation virulent strain of T. equiperdum infected 5 white mice with the isolated blood the infected rabbits (I – passage). Height of parasitemia in mice took blood were bled and injected it into the following party (II – passage) and carried out X passages. I passage of a trypanosome in blood of white mice appeared in single quantities (1–5 trypanosomes 1 f.o.v) for 4 days. Parasitic reaction increased till 20–65 1 f.o.v and killed mice for 21–24 days. Parasites of the II–VI – passages were found 3–4 days, their quantity has increased 90–95 1 f.o.v parasites. Mice killed for 6–7 days. VII–X passages significantly shorten the appearance of parasites in blood, for 3 days the parasitemia was 90-110 1 f.o.v trypanosomes. The death of mice was observed for 3–4 days.
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
The results demonstrated that the virulence of strain of T. equiperdum can be increased with VII – passage. http://dx.doi.org/10.1016/j.jbiotec.2016.05.257 Effects of size grading and size difference on growth performance of hybrid tilapia (Oreochromis niloticus ♀ X Oreochromis aureus♂) juvenile in fiber tank conditions Suat Dikel Aquaculture Department, Faculty of Fisheries, Cukurova University, Faculty of Fisheries, Adana, Turkey E-mail address: [email protected]. The effects of size grading and size differences between small and large individuals on growth performance of hybrid tilapia (Oreochromis niloticus ♀ X Oreochromis aureus♂) juvenile were investigated. The 0 age 1–4 g hybrid fry which have not completed the juvenile period were stocked as a 40 fry/m3 in 500 l fiber tanks placed in a greenhouse and cultured for 60 days. Trails comprises four groups, the first graded group (G1), consisting of only small individuals, and three mixed groups consisting 1 g and 2 g (G2), 1–3 g (G3) and 1–4 g (G4) juvenile respectively. At the end of the experimental feeding period, it was observed that small fry in G2 were reached the best growth performance among the other groups (P < 0.01). Feed conversion ratio of group G2 (1.39 ± 0.05) was lower than the other groups (P < 0.05). In conclusion, size grading and size difference were affected on growth performance of hybrid tilapia fry, especially when juvenile were stocked as 1–2 g stocking composition (like G2), as a result of well competition can be established and growth performance of trail positively affected that. http://dx.doi.org/10.1016/j.jbiotec.2016.05.258 Degradation of clofibric acid by a phenol resistant Pseudomonas aeruginosa strain Sabra Hemidouche 1,2,∗ , Lidia Favier 3 , Zahra Sadaoui 1 , Abdeltif Amrane 3 1
Laboratoire de Génie de la Réaction, Faculté de Génie Mécanique et de Génie des Procédés, Université des Sciences et de la Technologie Houari, Boumediene, BP n◦ 32 el Alia Bab -Ezzouar, 16111 Alger, Algeria 2 Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, BP 384, Siège ex-Pasna Zone Industrielle, Bou-Ismail, CP 42004 Tipaza, Algeria 3 Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, Université Européenne de Bretagne, 11 Allée de Beaulieu, CS 50837, 35708 Rennes Cedex 7, France E-mail address: [email protected] (S. Hemidouche).
This study provides an alternative solution for the bioremediation of clofibric acid (CLA) a human pharmaceutical, well known as refractory to the conventional wastewater treatment. The capacity of a phenol resistant Pseudomonas aeruginosa strain to remove CLA was investigated. Kinetic batch experiments were conducted in basal minimal medium under aerobic conditions and at different initial pollutant concentrations and in the presence or in the absence of a carbon co-substrate. For these tests the stirring
S71
rate, pH and temperature were fixed to 150 tr/min, 7.3 and 37 ◦ C, respectively. Samples were regularly taken over 7 days in order determine the optical density, pH and the pollutant concentration (by HPLC/UV). Results have shown that the bacterial metabolic activity is reflected by pH decreasing and consumptions of carbon co-substrates. It was observed that the CLA elimination yield ranges between 71% and 17%, depending on its initial concentration. http://dx.doi.org/10.1016/j.jbiotec.2016.05.259 Testing the killing ability of pig neutrophils after stimulation with an ethanolamine derivative Gabriel Gajaila ∗ , Iuliana Gajaila, Gabriel Cotor, Lucian Ionita Preclinical Sciences Department, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine, Bucharest, Romania E-mail address: [email protected] (G. Gajaila). The pig neutrophils are separated from blood samples by the use of density gradient with Percoll medium. The purified neutrophils are seeded into the walls of microtiter plates in a tissue culture medium supported with nutrients and buffering serum. The ethanolamine solution is added in the tissue culture and the tray is incubated at 37 ◦ C for 2 h in an optimum atmosphere. Viable staphylococci are used as phagocytosis target and reincubated with pig neutrophils such that an optimum number of these are taken into the cytoplasm of the phagocytic cells. After several incubation periods, the neutrophils can be removed from the culture well and lysed by exposure to water. The lysate was cultured in order to determine the number of viable staphylococci remaining at that time, relative to the total number of starting bacterial organisms. Viable staphylococci released from the cytoplasm may be cultured on agar plates and the number of colonies enumerated. Neutrophils will progressively kill an increasing numbers of staphylococci, so that there are fewer colonies at each time point of the experiment (30 min intervals over a two-hour period). The “killing curve” generated after the experiment allows the comparison of the phagocitary activity of normal and stimulated neutrophils. The results indicate a 30% decrease of the surviving staphylococci in the stimulated neutrophils vs. the unstimulated ones. http://dx.doi.org/10.1016/j.jbiotec.2016.05.260 Purification and characterization of a bioflocculant produced by Bacillus amyloliquefaciens AS21a Aynur Gul Karahan 1,∗ , Gizem Gunay 1 , Mehmet Lutfu Cakmakci 2 1
Suleyman Demirel University, Faculty of Engineering, Department of Food Engineering, Turkey 2 Ankara University, Faculty of Engineering, Department of Food Engineering, Turkey E-mail address: [email protected] (A.G. Karahan). In this study, 109 strains were isolated from samples of soil and waste water that were collected from I˙ zmir, Isparta, Antalya, Denizli, Balikesir and Bursa. Strains that will be determined the flocculating activities were investigated for their morphological