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Isolement des cellules souches mésenchymateuses autologues dérivées du tissu adipeux pour la réparation des pertes de substance osseuse
Revue de chirurgie orthopédique et traumatologique 102 (2016) 656
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Mémoire original
Isolement des cellules souches mésenchymateuses autologues dérivées du tissu adipeux pour la réparation des pertes de substance osseuse夽 Isolation of autologous adipose tissue-derived mesenchymal stem cells for bone repair E. Raposio a , S. Bonomini b , F. Calderazzi c,∗ a
Department of Surgical Sciences, Plastic Surgery Section, University of Parma, Via Gramsci 14, 43100 Parma, Italy Department of Clinical and Experimental Medicine, Division of Hematology, Parma University Hospital, Via Gramsci 14, 43100 Parma, Italy c Department of Surgical Sciences, Orthopedic Clinic, Parma University Hospital, Via Gramsci 14, 43100 Parma, Italy b
i n f o
a r t i c l e
Historique de l’article : Rec¸u le 31 janvier 2016 Accepté le 19 juillet 2016 Keywords: Adipose-derived stem cells Bone and cartilage Cell Therapy
a b s t r a c t Introduction. – Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. Methods. – This paper describes the procedure to obtain a 5 cc saline sample, containing the adiposederived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. Results. – A mean of 2.5 × 106 cells is isolated for each procedure; 35 % (875 000) of these are CD34 +/ CD45cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLA-ABC) and negative (CD33, CD39, CD102, CD106, CD146, HLA-DR) cell-associated surface antigens, characterizing them as ASCs. Conclusions. – This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures. © 2016 Elsevier Masson SAS. All rights reserved.
DOI de l’article original : http://dx.doi.org/10.1016/j.otsr.2016.07.006. 夽 Cet article peut être consulté in extenso dans la version anglaise de la revue Orthopaedics & Traumatology: Surgery & Research sur Science Direct (sciencedirect.com) en utilisant le DOI ci-dessus. ∗ Auteur correspondant. Adresse e-mail : fi[email protected] (F. Calderazzi). http://dx.doi.org/10.1016/j.rcot.2016.07.022 ´ ´ 1877-0517/© 2016 Elsevier Masson SAS. Tous droits reserv es.
Disponible en ligne sur
ScienceDirect www.sciencedirect.com
Mémoire original
Isolement des cellules souches mésenchymateuses autologues dérivées du tissu adipeux pour la réparation des pertes de substance osseuse夽 Isolation of autologous adipose tissue-derived mesenchymal stem cells for bone repair E. Raposio a , S. Bonomini b , F. Calderazzi c,∗ a
Department of Surgical Sciences, Plastic Surgery Section, University of Parma, Via Gramsci 14, 43100 Parma, Italy Department of Clinical and Experimental Medicine, Division of Hematology, Parma University Hospital, Via Gramsci 14, 43100 Parma, Italy c Department of Surgical Sciences, Orthopedic Clinic, Parma University Hospital, Via Gramsci 14, 43100 Parma, Italy b
i n f o
a r t i c l e
Historique de l’article : Rec¸u le 31 janvier 2016 Accepté le 19 juillet 2016 Keywords: Adipose-derived stem cells Bone and cartilage Cell Therapy
a b s t r a c t Introduction. – Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. Methods. – This paper describes the procedure to obtain a 5 cc saline sample, containing the adiposederived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. Results. – A mean of 2.5 × 106 cells is isolated for each procedure; 35 % (875 000) of these are CD34 +/ CD45cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLA-ABC) and negative (CD33, CD39, CD102, CD106, CD146, HLA-DR) cell-associated surface antigens, characterizing them as ASCs. Conclusions. – This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures. © 2016 Elsevier Masson SAS. All rights reserved.
DOI de l’article original : http://dx.doi.org/10.1016/j.otsr.2016.07.006. 夽 Cet article peut être consulté in extenso dans la version anglaise de la revue Orthopaedics & Traumatology: Surgery & Research sur Science Direct (sciencedirect.com) en utilisant le DOI ci-dessus. ∗ Auteur correspondant. Adresse e-mail : fi[email protected] (F. Calderazzi). http://dx.doi.org/10.1016/j.rcot.2016.07.022 ´ ´ 1877-0517/© 2016 Elsevier Masson SAS. Tous droits reserv es.