Biosensors & Bioelectronics VoL 12 No. 5 (1997)
determination of phosphate, taking approximately 3 mm for one measurement. A linear response was observed from 0.37 gM to 7.4 gM phosphate while the detection limit was 74 nM. This sensitivity is sufficient to determine the maximum permissible phosphate concentration of the natural waters of Japan (0.32 gM). The pyruvate oxidase immobilized column gave a stable response over a period of 2 weeks when stock solutions were analyzed.
Contact: Research Center, Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153, Japan. Italy - Potentiometric alternating biosensing In J. BIOCHEM. BIOPHYS. METHODS (32/3 (171-181) 1996) M. Adami, I. Zolfmo, S. Fenu, D. Nardelli & C. Nicolini of Societa Ricerca Industriale Avanzatareport on 'Potentiometric alternating biosensing system for in continuum toxicity tests on cell population'. The possibility to monitor, in continuum, changes of cellular metabolism was investigated. Examples of this feature are shown: metabolic changes due to drugs administration are evaluated as a decrease of extra-cellular acidification rate on CHO-K1 and HeLa cells. Parallel experiments with a standard test were performed to validate the results obtained with the biosensor.
Contact: Technobiochip, Societa Ricerca Industriale Avanzata, Via della Marina, 1-57030 Marciana, Italy. Japan - Sensor for monitoring river water In J. BIOTECHNOL. (48/1-2 (73-80) 1996) K. lkebukuro, A. Miyata, S.J. Cho, Y. Nomura, S . M Chang, Y. Yamauchi, Y. Hasebe, S. Uchiyama & I. Karube of the University of Tokyo report on 'Microbial cyanide sensor for monitoring river water'. A microbial cyanide sensor using Saccharomyces cerevisiae for monitoring a river water is described. This sensor is based on the inhibition of S. cerevisiae's respiration by cyanide. This sensor is a reactor type flow system and composed of two oxygen electrodes and a reactor which contains S. cerevisiae immobilized beads. The S cerevisiae's respiration activity is measured using the oxygen electrodes. The sensor showed a linear response in the range from 0 to 15 gM and maintained stable response for 9 days at ambient temperature. The sensor was optimized for the monitoring of river water and was applied to river water analysis.
Contact: Research Center, Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-lcu, Tokyo 153, Japan.
These abstracts are sourced from the Excerpta Mediea database Embase. No responsibilityis assumed by Elsevier Advanced Technologyfor misinterpretationor damageor costs directlyor indirectlydue to grammaticalor textural errors containedtherein; nor for any injury and/or damage to persons or propertyas a matter of products liability,negligenceor otherwise,or from any use or operationof any methods,products,inslructionsor ideascontainedin the material herein. Because of rapid advances in the medical sciences,we recommendthat independentverificationof diagnosesand drag dosages should be made: EAT makes no representation or warranty expressed or implied, includingbut not limitedto any impliedwarranty or merchantabilityof fitness for a particular purpose, with respect to this section and specificallydisclaimsall such warrantiesand representations.
0956-5663/97/$17.00 © 1997 Elsevier Science Ltd
vii