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JOURNAL SCAN
JOURNAL SCAN Barberis MCP, Faleri M, Verones e S , Casadi o C, Viale G. A selectiv e marker of normal an d neoplasti c mesothelia l cells in serou s effusions . Acta Cytol. 1997 ; 41(6) : 1757-61 . This study was undertaken to asses s the utility of a specifi c immunohistochemica l marker - calretinin - for accurate distinction betwee n mesothelioma s and metastati c carcinoma s in malignant serou s effusions . At presen t no definite marker exist s to make-this distinction with certainity. The authors studie d 33 cytologic specimen s of serou s effusion s - 2 0 from mesothelioma s and 13 from metastati c adenocarcinomas . This was a retrospective study of case s whos e diagnosi s had bee n establishe d by histolog y (light and electro n microscopically) . The workers found that all 20 specimen s from mesothelioma s as well as normal mesothelia l cells staine d strongly and diffusely { > 75 % cells ) with calretinin. Only 3/1 3 case s of metastati c adenocarcinom a showe d faint reactivity in 10-5 0 pe r cen t cells . Similar results were als o found by immuno-electronmicroscopy . The authors conclud e that calretinin show s 10 0 pe r cen t sensitivity and 81 pe r cen t specificity for mesothelia l cells . It's us e along with markers of adenocarcinom a like CEA and B72. 3 will considerabl y improve the diagnosti c accurac y in case s of mesothelioma .
D'Costa M , Fleming E, Patterson MC. Cardiac Troponin-I for the Diagnosis of Acute Myocardial Infarction in the Emergency Department. Am .1 Clin Pathol 1997: 108: 550-5. Cardiac troponin-I (Tnl) was tested in 316 consecutive patients admitted with chest pain to the emergency department of a medium-sized community based hospital. The aim o f the study was to compare the rate o f positive finding for Tnl and CK-MB at admission and at 24 hrs. Serum myoglobin an early marker or A M I was also measured. Of the 316 patients 62 were discharged with a diagnosis of acute myocardial infarction. Forty nine of them showed an abnormal level of Tnl compared with 27 for creatine kinase (CK-MB), in the first specimen obtained at admission. The overall peak performance ofTnl testing in samples at 24 hours of admission indicated a high sensitivity (97%) and specificity (98%) for the diagnosis of A M I . A l l 62 patients with A M I were correctly diagnosed when a combination of the Tnl and myoglobin was used. A further advantage recorded by the authors was in elderly patients with myocardial injury and low CK and normal CK-MB values where Tnl was positive. The authors suggest that testing for Tnl could replace CK-MB and in combination with myoglobin, could facilitate the rapid and effective triage of patients with chest pain in the emergency department.
Schocll WMJ. Pieber D, Reich O, Lahouscn M , Janicek M , Guecer F. Winter R. Tumor angiogenesis as a prognostic factor in ovarian carcinoma - Quantification of Endothelial immunorcactivity by image analysis. Cancer. 1997; 80(12): 2257-62.
Contributed by Wg Cdr (Mrs)AK Sabhikhi. Reader. Department of Pathology, Armed Forces Medical College. Pune 411040
The authors undertook to objectively quantify tumor microvessel density based on the premise that neovascularisation would correlate with the degree o f proliferation and hence the prognosis of ovarian carcinoma. They studied 24 patients of FIGO Stage 111c ovarian carcinomas who remained alive and free of disease 6 years after diagnosis and treatment. The control group consisted of 14 patients o f ovarian carcinoma matched for age. stage, diagnosis, treatment etc, but who died during this period. Tumor tissue from the biopsy at diagnosis was stained with antibodies to FVIII, CD 31 and CD 34 as endothelial markers using the alkaline phosphatasc-streptavidin biotin method for immunohistochemical staining. CD 34 stained sections were further analysed by a computer-aided image analysis system. The authors noted that the endothelial area in the survivors was only 33 per cent o f the area in those who died and suggest a cut-off value of 0.05 mm of endotheliac area for a specificity of 79 per cent and a sensitivity of 93 per cent. The authors conclude that in such matched groups under study, endothelial area proved to be an independant prognostic factor in patients with advanced ovarian carcinoma.
Bauemifein d A. Compariso n of antibacterial activities of the quinolones , Bay-12-8039 , gatifloxacin (AM 1155) , trovafloxacin, clinafloxacin, levofloxaci n an d ciprofloxacin. Journal of Antimicrobial Chemotherap y (1997) ; 40:639-51 .
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Contributed by Lt Col (Mrs) R Lakhtakia, Reader, Department of Pathology, Armed Forces Medical College, Pune 411040
Recentl y a numbe r of quinolone s like Bay 12-8039 , gatifloxacin, trovafloxacin, clinafloxacin and levofloxaci n have reache d to the clinical investigations . Antibacterial activity of thes e five newe r quinolone s and of well establishe d ciprofloxacin was determine d agains t two thousan d two hundred and ninety four isolate s of eighty seve n species . MICs were determine d by the aga r dilution technique . The majority of Enterobacteriacea e has MICs of <1 mg/L of all six quinolones . Clinafloxacin wa s mos t active compoun d agains t all specie s being two to four times more active than ciprofloxacin, Bay 12-8039 , gatifloxacin and four to eigh t times active than levofloxaci n and trovafloxacin. Activity agains t glucos e no n fermenting Gram negativ e rods varies betwee n species . Agains t Pseudomonas aeruginosa and Ps putida the mos t active compound s were : ciprofloxacin and clinafloxacin (MIC= 0.5- 2 mg/L). Most active compoun d agains t staphylococcu s were Bay 12-8039.trovafloxaci n and clinafloxacin (MICgo = 0.06-0.25mg/L ) followed by gatifloxacin (MICgo= 0.13-0.5mg/L) , levofloxaci n (MICo= 0.2 5 - 1mg/L) and ciprofloxacin (MICgo= 0.5- 1 mg/L). Agains t enterococc i Bay 9
Contributed by Lt Col (Mrs R Lakhatakia, Wg Cdr AK Sabhikhi, Department of Pathology; Lt col Yogesh Chandcr. Dept of Microbiology. A F M C . Pune
D'Costa M , Fleming E, Patterson MC. Cardiac Troponin-I for the Diagnosis of Acute Myocardial Infarction in the Emergency Department. Am .1 Clin Pathol 1997: 108: 550-5. Cardiac troponin-I (Tnl) was tested in 316 consecutive patients admitted with chest pain to the emergency department of a medium-sized community based hospital. The aim o f the study was to compare the rate o f positive finding for Tnl and CK-MB at admission and at 24 hrs. Serum myoglobin an early marker or A M I was also measured. Of the 316 patients 62 were discharged with a diagnosis of acute myocardial infarction. Forty nine of them showed an abnormal level of Tnl compared with 27 for creatine kinase (CK-MB), in the first specimen obtained at admission. The overall peak performance ofTnl testing in samples at 24 hours of admission indicated a high sensitivity (97%) and specificity (98%) for the diagnosis of A M I . A l l 62 patients with A M I were correctly diagnosed when a combination of the Tnl and myoglobin was used. A further advantage recorded by the authors was in elderly patients with myocardial injury and low CK and normal CK-MB values where Tnl was positive. The authors suggest that testing for Tnl could replace CK-MB and in combination with myoglobin, could facilitate the rapid and effective triage of patients with chest pain in the emergency department.
Schocll WMJ. Pieber D, Reich O, Lahouscn M , Janicek M , Guecer F. Winter R. Tumor angiogenesis as a prognostic factor in ovarian carcinoma - Quantification of Endothelial immunorcactivity by image analysis. Cancer. 1997; 80(12): 2257-62.
Contributed by Wg Cdr (Mrs)AK Sabhikhi. Reader. Department of Pathology, Armed Forces Medical College. Pune 411040
The authors undertook to objectively quantify tumor microvessel density based on the premise that neovascularisation would correlate with the degree o f proliferation and hence the prognosis of ovarian carcinoma. They studied 24 patients of FIGO Stage 111c ovarian carcinomas who remained alive and free of disease 6 years after diagnosis and treatment. The control group consisted of 14 patients o f ovarian carcinoma matched for age. stage, diagnosis, treatment etc, but who died during this period. Tumor tissue from the biopsy at diagnosis was stained with antibodies to FVIII, CD 31 and CD 34 as endothelial markers using the alkaline phosphatasc-streptavidin biotin method for immunohistochemical staining. CD 34 stained sections were further analysed by a computer-aided image analysis system. The authors noted that the endothelial area in the survivors was only 33 per cent o f the area in those who died and suggest a cut-off value of 0.05 mm of endotheliac area for a specificity of 79 per cent and a sensitivity of 93 per cent. The authors conclude that in such matched groups under study, endothelial area proved to be an independant prognostic factor in patients with advanced ovarian carcinoma.
Bauemifein d A. Compariso n of antibacterial activities of the quinolones , Bay-12-8039 , gatifloxacin (AM 1155) , trovafloxacin, clinafloxacin, levofloxaci n an d ciprofloxacin. Journal of Antimicrobial Chemotherap y (1997) ; 40:639-51 .
2
Contributed by Lt Col (Mrs) R Lakhtakia, Reader, Department of Pathology, Armed Forces Medical College, Pune 411040
Recentl y a numbe r of quinolone s like Bay 12-8039 , gatifloxacin, trovafloxacin, clinafloxacin and levofloxaci n have reache d to the clinical investigations . Antibacterial activity of thes e five newe r quinolone s and of well establishe d ciprofloxacin was determine d agains t two thousan d two hundred and ninety four isolate s of eighty seve n species . MICs were determine d by the aga r dilution technique . The majority of Enterobacteriacea e has MICs of <1 mg/L of all six quinolones . Clinafloxacin wa s mos t active compoun d agains t all specie s being two to four times more active than ciprofloxacin, Bay 12-8039 , gatifloxacin and four to eigh t times active than levofloxaci n and trovafloxacin. Activity agains t glucos e no n fermenting Gram negativ e rods varies betwee n species . Agains t Pseudomonas aeruginosa and Ps putida the mos t active compound s were : ciprofloxacin and clinafloxacin (MIC= 0.5- 2 mg/L). Most active compoun d agains t staphylococcu s were Bay 12-8039.trovafloxaci n and clinafloxacin (MICgo = 0.06-0.25mg/L ) followed by gatifloxacin (MICgo= 0.13-0.5mg/L) , levofloxaci n (MICo= 0.2 5 - 1mg/L) and ciprofloxacin (MICgo= 0.5- 1 mg/L). Agains t enterococc i Bay 9
Contributed by Lt Col (Mrs R Lakhatakia, Wg Cdr AK Sabhikhi, Department of Pathology; Lt col Yogesh Chandcr. Dept of Microbiology. A F M C . Pune