- Email: [email protected]
Karyotypic comparison of a testicular cancer cell line with acquired resistance to cis-platin and its sensitive counterpart
Abstracts
255
. A27
KARYOTYPIC COMPARISON OF A TESTICULAR CANCER CELL LINE WITH ACQUIRED RESISTANCE TO CIS-PLATIN AND ITS SENSITIVE COUNTERPART. NA Heerema. BJ Roth, CG Palmer, A Milatovich, GW Sledge, Jr.. IN Univ School of Medicine, Indianapolis, IN A cell line (833K ~ ) resistant to 64xi05 ug/ml cisplatin was developed from the testicular tumor cell line 833K. Karyotypic analysis of the cell lines showed 3 clones in the sensitive line, all related and showing clonal evolution The resistant line is monoclonal and shows further changes from the karyotypically m O ~ c ~ b n o r m a l sensitive clone. The k a r y o t y p ~ & ~ 833K ~ with differences between 833K
8) of t
.i) ss
cellular glutathi stainlng followed the 2 cell lines, levels by 6 hour (S,R)-sulfoxlmine extremely long sa are translocated tono~acro~elntrlcchromsomes. However, the amount ~flI!NOR:i~!~ii~ty: !~S ir~¢&~led by AgNOR staining is comparable in both the sensitive and the resistant lines.
255
. A27
KARYOTYPIC COMPARISON OF A TESTICULAR CANCER CELL LINE WITH ACQUIRED RESISTANCE TO CIS-PLATIN AND ITS SENSITIVE COUNTERPART. NA Heerema. BJ Roth, CG Palmer, A Milatovich, GW Sledge, Jr.. IN Univ School of Medicine, Indianapolis, IN A cell line (833K ~ ) resistant to 64xi05 ug/ml cisplatin was developed from the testicular tumor cell line 833K. Karyotypic analysis of the cell lines showed 3 clones in the sensitive line, all related and showing clonal evolution The resistant line is monoclonal and shows further changes from the karyotypically m O ~ c ~ b n o r m a l sensitive clone. The k a r y o t y p ~ & ~ 833K ~ with differences between 833K
8) of t
.i) ss
cellular glutathi stainlng followed the 2 cell lines, levels by 6 hour (S,R)-sulfoxlmine extremely long sa are translocated tono~acro~elntrlcchromsomes. However, the amount ~flI!NOR:i~!~ii~ty: !~S ir~¢&~led by AgNOR staining is comparable in both the sensitive and the resistant lines.