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Low Wall shear Stress
L 005
Aorta Remodeling Responses to Distinct Atherogenic Stimuli: Hypertension, Hypercholesterolemia and Turbulent Flow/Low Wall shear Stress
L 007
HDL-Cholesterol (HDL-c) Plasma Concentration in No-Diabetics Women with Coronary Artery Disease (CAD) or Not. Is There Difference?
Prado CM, Rossi MA
Denardi CAS, Chagas ACP, Casella Filho A, Favarato D, Luz PL
Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto – Famesp, Universidadae de São Paulo, São Paulo, SP, Brazil
Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
Background: Taking into account that atherosclerosis is a focal disease and high levels of plasma cholesterol and blood pressure are closely correlated with its pathogenesis, it is a challenge to explain local rather than global effects on arteries. We investigated the role of hypertension, hypercholesterolemia and turbulent blood flow /low wall shear stress on aorta remodeling of rats feeding normal or hypercholesterolemic diet after severe stenosis of the abdominal aorta. Methods: Animals were divided in sham-operated, operated, sham-operated + hypercholesterolemic diet (HD), and operated + HD. Results: This model of aortic constriction produced: (a) hypertensive prestenotic segment associated with laminar flow/normal wall shear stress characterized by enlarged heterogeneous endothelial cells, diffusely distributed neointimal plaques, and medial thickening; (b) normotensive poststenotic segment associated with turbulent flow/low wall shear stress characterized by intima delicate and focally distributed neointimal plaques similar to those observed in the hypertensive prestenotic segment but many of them larger in size; (c) hypercholesterolemic prestenotic segment revealed diffusely distributed foci of small flat lesions characterized by intimal foam cells accumulation, and (d) hypercholesterolemic poststenotic segment revealed focally distributed incipient atherosclerotic lesions characterized by raised focal lesions within the intima. Conclusions: Our findings suggest that increased circumferential wall tension due to hypertension plays a key role in the remodeling through biomechanical effects on oxidative stress and increased TGF-a expression. The remodeling observed in the presence of hypercholesterolemia could be initiated by oxidative stress that is involved in several processes of atherogenesis and this remodeling is more pronounced in the presence of turbulent blood flow/low wall shear stress.
Purpose: The cardiovascular diseases have presented great increase contributing in the current days by 78% of all causes of death in the developing countries. In reason of its increasing impact in women in the last few decades and, considering dyslipidemia a factor of important risk in this population, our objective was to evaluate HDL-c plasma concentration and statin treatment of the no-diabetics women with coronary artery disease (CAD) or not in an university hospital. Methods: Observed study in women with CAD were collected and evaluated in the hospital laboratory in 2008/2009. The data were collected during the routine consultations by the investigator. Results: From a group with 154 women patients with ages between 26 and 97 years old, we observed 2 groups: From a group with 90 patients with CAD (ages between 44 and 97 years old), we observed: 57 (63.3%) with HDL-c < 50 mg/dL and 26 (28.8%) HDL-c < 40 mg/dL and 11 (12.2%) without statin use. From the group without CAD (64 patients with ages between 26 and 83 years old), we observed: 36 (56.3%) with HDL-c < 50 mg/dL and 12 (18.8%) HDL-c < 40 mg/dL and 40 (62.5%) without statin use. The frequency of HDL-c level were simmilar in both groups to consider HDL-c < 50 mg/dL (p = 0.4724), and HDL-c < 40 mg/dL (p = 0.2118). The regular use of statin were statistical significant (37.5% vs. 788%; OR= 0.23; IC95% (0.1-0.56); p = 0.0004). Conclusions: There weren’t differences in HDL-c < 50 mg/dL (p = 0.4724) or HDL-c< 40 mg/dL (p = 0.2118). The regular use of statin were statistical significant (37.5% vs. 78.8%; OR = 0.23; IC95% (0.1-0.56); p = 0.0004).
L 006
HDL-Cholesterol (HDL-C) Plasma Concentration in no- Diabetic Women with Coronary Artery Disease (CAD) Submitted to Coronary Artery Bypass Graft (CABG) and/or Percutaneous Coronary Intervention (PCI) and Clinic Treatment. Is There Difference?
Denardi CAS, Chagas ACP, Casella Filho A, Favarato D, Luz PL Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil Purpose: The cardiovascular diseases have presented great increase contributing in the current days by 78% of all causes of death in the developing countries. In reason of its increasing impact in women in the last few decades and, considering dyslipidemia as a factor of important risk, in this population our objective was to evaluate HDL-c plasma concentration and statin treatment in the women with coronary artery disease (CAD), submitted Coronary artery bypass graft (CABG) and/or percutaneous coronary intervention (PCI) and clinic treatment in an university hospital. Methods: Observed study in women with CAD were collected and evaluated in the hospital laboratory in 2008/2009. The data were collected during the routine consultations by the investigator. Results: From a group of 90 women patients with CAD with ages between 44 and 97 years old, 68 patients submitted to CABG and/or PTCA, we observed: 45 (66%) patients with HDL-c < 50 mg/dL and 22 (32.3%) with HDL ≤ 40 mg/dL and 7 (10.2%) without statin therapy. PCI = 35 (51.4%), CABG = 30 (44.1%) and CABG + PCI = 3 (4.4%) and in this last group HDL-c <50 mg/dL, in statin regular use. From a group in clinic treatment, 22 patients (ages between 45 and 96 years old), we observed: 15 (68.1%) patients with HDL-c < 50 mg/dL and 7 (31.8%) HDL-c < 40 mg/dL and 4 (18%) without statin therapy. Conclusions:There were not differences in HDL-c < 50 mg/dL (p = 0.93) or HDL-cl < 40 mg/dL (p = 0.13), and the regular use of statin is not statistical significance (p = 0.456).
32
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LIPIDS
L 008
Functional Characteristics of Plasma Lipoproteins From Patients with Metabolic Syndrome are Modulated by Changes in Their Size and Composition After Short-term Exercise
Casella Filho A, Cesena FHY, Trombetta IC, Denardi CAS, Dourado PMM, Silva VM, Negrão CE, Maranhão RC, Luz PL, Chagas ACP Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil Purpose: To verify whether modulation of functional properties of lipoproteins by short-term exercise training (T) is associated with changes in size and composition of LDL and HDL subfractions in patients with metabolic syndrome (MetS). Methods: Forty sedentary persons (30 with MetS, 10 controls) were evaluated. Twenty of those with MetS were subjected to a 3 times/week controlled training load (45 min/day) for 3 months on a bicycle ergometer. LDL and HDL subfractions were obtained by plasma ultracentrifugation, before and after T, and their compositions were analyzed. HDL particle size was assessed by laser-light scattering method, HDL acceptor property by transfer of radioactive labeled lipids, and PON1 activity was determined. In vitro resistance of LDL to oxidation with CuS04 was evaluated. LDL from control subjects was incubated with HDL2a or 3b from MetS patients (before and after T) and the resistance to oxidation was verified. Results: T did not alter plasma levels of total cholesterol (TC), LDL-c, HDL-c, apoA1 and apoB but significantly decreased the concentration of triglycerides (TG). LDL resistance to oxidation increased (+91%) after T, which was associated with a significant decrease only in the content of apoB (–16%) and TG (–14%) in the LDL particle. Oxidizability of control LDL decreased when mixed with HDL2a or 3b from patients with MetS before and after T (–23% for HDL2a and-18% for HDL3b). This was associated with a significant decrease only in the content of TC and TG in HDL3b (–7% and –12%, respectively) and HDL3c (–13% and –15%, respectively). T did not change HDL particle size (10.23 ± 1.10 vs 10.26 ± 1.09 nm before and after T, respectively, p > 0.05), but increased free cholesterol (free-C) transfer to HDL (+6.8%) and PON1 activity (+21.4%) in MetS group. Conclusions: T does not change plasma LDL-c concentration but reduces LDL vulnerability to oxidation, associated with a decrease in TG and apoB content in LDL, indicating a change from
XII Brazilian Congress of Atherosclerosis
Aorta Remodeling Responses to Distinct Atherogenic Stimuli: Hypertension, Hypercholesterolemia and Turbulent Flow/Low Wall shear Stress
L 007
HDL-Cholesterol (HDL-c) Plasma Concentration in No-Diabetics Women with Coronary Artery Disease (CAD) or Not. Is There Difference?
Prado CM, Rossi MA
Denardi CAS, Chagas ACP, Casella Filho A, Favarato D, Luz PL
Departamento de Patologia, Faculdade de Medicina de Ribeirão Preto – Famesp, Universidadae de São Paulo, São Paulo, SP, Brazil
Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
Background: Taking into account that atherosclerosis is a focal disease and high levels of plasma cholesterol and blood pressure are closely correlated with its pathogenesis, it is a challenge to explain local rather than global effects on arteries. We investigated the role of hypertension, hypercholesterolemia and turbulent blood flow /low wall shear stress on aorta remodeling of rats feeding normal or hypercholesterolemic diet after severe stenosis of the abdominal aorta. Methods: Animals were divided in sham-operated, operated, sham-operated + hypercholesterolemic diet (HD), and operated + HD. Results: This model of aortic constriction produced: (a) hypertensive prestenotic segment associated with laminar flow/normal wall shear stress characterized by enlarged heterogeneous endothelial cells, diffusely distributed neointimal plaques, and medial thickening; (b) normotensive poststenotic segment associated with turbulent flow/low wall shear stress characterized by intima delicate and focally distributed neointimal plaques similar to those observed in the hypertensive prestenotic segment but many of them larger in size; (c) hypercholesterolemic prestenotic segment revealed diffusely distributed foci of small flat lesions characterized by intimal foam cells accumulation, and (d) hypercholesterolemic poststenotic segment revealed focally distributed incipient atherosclerotic lesions characterized by raised focal lesions within the intima. Conclusions: Our findings suggest that increased circumferential wall tension due to hypertension plays a key role in the remodeling through biomechanical effects on oxidative stress and increased TGF-a expression. The remodeling observed in the presence of hypercholesterolemia could be initiated by oxidative stress that is involved in several processes of atherogenesis and this remodeling is more pronounced in the presence of turbulent blood flow/low wall shear stress.
Purpose: The cardiovascular diseases have presented great increase contributing in the current days by 78% of all causes of death in the developing countries. In reason of its increasing impact in women in the last few decades and, considering dyslipidemia a factor of important risk in this population, our objective was to evaluate HDL-c plasma concentration and statin treatment of the no-diabetics women with coronary artery disease (CAD) or not in an university hospital. Methods: Observed study in women with CAD were collected and evaluated in the hospital laboratory in 2008/2009. The data were collected during the routine consultations by the investigator. Results: From a group with 154 women patients with ages between 26 and 97 years old, we observed 2 groups: From a group with 90 patients with CAD (ages between 44 and 97 years old), we observed: 57 (63.3%) with HDL-c < 50 mg/dL and 26 (28.8%) HDL-c < 40 mg/dL and 11 (12.2%) without statin use. From the group without CAD (64 patients with ages between 26 and 83 years old), we observed: 36 (56.3%) with HDL-c < 50 mg/dL and 12 (18.8%) HDL-c < 40 mg/dL and 40 (62.5%) without statin use. The frequency of HDL-c level were simmilar in both groups to consider HDL-c < 50 mg/dL (p = 0.4724), and HDL-c < 40 mg/dL (p = 0.2118). The regular use of statin were statistical significant (37.5% vs. 788%; OR= 0.23; IC95% (0.1-0.56); p = 0.0004). Conclusions: There weren’t differences in HDL-c < 50 mg/dL (p = 0.4724) or HDL-c< 40 mg/dL (p = 0.2118). The regular use of statin were statistical significant (37.5% vs. 78.8%; OR = 0.23; IC95% (0.1-0.56); p = 0.0004).
L 006
HDL-Cholesterol (HDL-C) Plasma Concentration in no- Diabetic Women with Coronary Artery Disease (CAD) Submitted to Coronary Artery Bypass Graft (CABG) and/or Percutaneous Coronary Intervention (PCI) and Clinic Treatment. Is There Difference?
Denardi CAS, Chagas ACP, Casella Filho A, Favarato D, Luz PL Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil Purpose: The cardiovascular diseases have presented great increase contributing in the current days by 78% of all causes of death in the developing countries. In reason of its increasing impact in women in the last few decades and, considering dyslipidemia as a factor of important risk, in this population our objective was to evaluate HDL-c plasma concentration and statin treatment in the women with coronary artery disease (CAD), submitted Coronary artery bypass graft (CABG) and/or percutaneous coronary intervention (PCI) and clinic treatment in an university hospital. Methods: Observed study in women with CAD were collected and evaluated in the hospital laboratory in 2008/2009. The data were collected during the routine consultations by the investigator. Results: From a group of 90 women patients with CAD with ages between 44 and 97 years old, 68 patients submitted to CABG and/or PTCA, we observed: 45 (66%) patients with HDL-c < 50 mg/dL and 22 (32.3%) with HDL ≤ 40 mg/dL and 7 (10.2%) without statin therapy. PCI = 35 (51.4%), CABG = 30 (44.1%) and CABG + PCI = 3 (4.4%) and in this last group HDL-c <50 mg/dL, in statin regular use. From a group in clinic treatment, 22 patients (ages between 45 and 96 years old), we observed: 15 (68.1%) patients with HDL-c < 50 mg/dL and 7 (31.8%) HDL-c < 40 mg/dL and 4 (18%) without statin therapy. Conclusions:There were not differences in HDL-c < 50 mg/dL (p = 0.93) or HDL-cl < 40 mg/dL (p = 0.13), and the regular use of statin is not statistical significance (p = 0.456).
32
/
LIPIDS
L 008
Functional Characteristics of Plasma Lipoproteins From Patients with Metabolic Syndrome are Modulated by Changes in Their Size and Composition After Short-term Exercise
Casella Filho A, Cesena FHY, Trombetta IC, Denardi CAS, Dourado PMM, Silva VM, Negrão CE, Maranhão RC, Luz PL, Chagas ACP Instituto do Coração – InCor – Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil Purpose: To verify whether modulation of functional properties of lipoproteins by short-term exercise training (T) is associated with changes in size and composition of LDL and HDL subfractions in patients with metabolic syndrome (MetS). Methods: Forty sedentary persons (30 with MetS, 10 controls) were evaluated. Twenty of those with MetS were subjected to a 3 times/week controlled training load (45 min/day) for 3 months on a bicycle ergometer. LDL and HDL subfractions were obtained by plasma ultracentrifugation, before and after T, and their compositions were analyzed. HDL particle size was assessed by laser-light scattering method, HDL acceptor property by transfer of radioactive labeled lipids, and PON1 activity was determined. In vitro resistance of LDL to oxidation with CuS04 was evaluated. LDL from control subjects was incubated with HDL2a or 3b from MetS patients (before and after T) and the resistance to oxidation was verified. Results: T did not alter plasma levels of total cholesterol (TC), LDL-c, HDL-c, apoA1 and apoB but significantly decreased the concentration of triglycerides (TG). LDL resistance to oxidation increased (+91%) after T, which was associated with a significant decrease only in the content of apoB (–16%) and TG (–14%) in the LDL particle. Oxidizability of control LDL decreased when mixed with HDL2a or 3b from patients with MetS before and after T (–23% for HDL2a and-18% for HDL3b). This was associated with a significant decrease only in the content of TC and TG in HDL3b (–7% and –12%, respectively) and HDL3c (–13% and –15%, respectively). T did not change HDL particle size (10.23 ± 1.10 vs 10.26 ± 1.09 nm before and after T, respectively, p > 0.05), but increased free cholesterol (free-C) transfer to HDL (+6.8%) and PON1 activity (+21.4%) in MetS group. Conclusions: T does not change plasma LDL-c concentration but reduces LDL vulnerability to oxidation, associated with a decrease in TG and apoB content in LDL, indicating a change from
XII Brazilian Congress of Atherosclerosis