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Lack of association of the G-protein subunit gene and left ventrucular hypertrophy
AJH–April 2001–VOL. 14, NO. 4, PART 2
P-133 LACK OF ASSOCIATION OF THE G-PROTEIN SUBUNIT GENE AND LEFT VENTRUCULAR HYPERTROPHY
POSTERS: Genetics/Gene Therapy
II DD ID
75A
CREA 0
Crea 24
⌬ Crea
1,38⫹/- 0,15 1,70⫹/-0,11* 1,33⫹/- 0,15
1,39⫹/-0,28 1,48⫹/-0,16 1,42⫹/-0,16
-0,01⫹/- 0,17 0,23⫹/-0,08* -0,09⫹/- 0,16
Vladimir A. Almasov, Eugene V. Shlyakhto, Eugene I. Shwartz, Anna V. Zucova, Alexandra O. Conrady, Tatyana A. Vinnic. 1Faculty Therapy Department, Pavlov State Medical University, St.Petersburg, Russia
Key Words: antihypertensive drugs, polymorphism, Angiotensine converting enzyme
The aim of the present study was to determine if there is an association of G-protein 3 subunite (GNB3) gene polymorphisms and left ventricular hypertrophy (LVH) in patients with essential hypertension (EH) in St.Petersburg population. We examined 135 patients (the mean age 48⫾7yrs) with mild to moderate EH recruited from the general population of the outpatient hypertensive clinic. Left ventricular mass was measured by echocardiography and left ventricular mass index (LVMI) was calculated. The GNB3 C825T genotype was determined by polymerase chain reaction and restriction digestion. 67 patients (0.50) were homozygous for the C allele (CC), 56 were heterozygous (CT) (0.41) and twelve (0.09) were homozygous for T allele (TT). The distribution of genotypes among the patients was in Hardy-Weinberg equilibrium and did not differ significantly when comparing patients with or without LVH. The frequency of T allele was only slightly higher in patients with LVH (0,32) compared to ones without LVH (0.28) and LVMI was similar in patients with CC, CT and TT genotype (120.2⫾13.4, 120.4⫾13.6, 122.3⫾12.4 g/m2, respectively). In the light of these observations it seems reasonable to conclude that no association of LVH and CNB3 gene variant has been found in the population studied.
P-135 OBESITY, INSULIN RESISTANCE, CARDIAC STRUCTURE, AND RENIN-ANGIOTENSIN SYSTEM GENES IN ESSENTIAL HYPERTENSION
Key Words: Genetics, G-protein, Left ventricular hypertrophy
P-134 DOES ACE POLYMORPHISM INFLUENCE BLOOD PRESSURE AND S-CREATININE UNDER ACEINHIBITOR TREATMENT? Barbara M. Suwelack, Uta Hillebrand, Ulf M.W. Gerhardt, Manuela Kempkes, Karl H. Rahn, Helge Hohage. 1Medizinische Poliklinik, Universita¨t Mu¨nster, Mu¨nster, Germany Aim&Scope: We know 3 different genotypes of the ACE gene, DD, ID and II (I⫽Insertion / D⫽ Deletion). The present study tests the hypothesis that different ACE genotypes may show differences under ACE inhibition. Material and methods: In 3 vs. 4 vs. 9 kidney transplant recipients with genotype II, DD and ID the s-creatinine and the arterial blood pressure were estimated before and after 24 months of ACE inhibitor treatment. Results: The tables show that, unexpectedly, the arterial blood pressure is significantly reduced only in the II group. On the other hand, s-creatinine decreased significantly only in the DD group. (see table) In Group II, the MAD decreased from 114,78⫹/-1,44 mmHg to 93,00⫹/-3,34 mmHg (⌬MAP 21,78⫹/-3,76 mmHg; p⬍0.05). However no significant MAP reduction was found in DD (⌬MAP 0,67⫹/-4,89 mmHg) or ID (⌬ MAP 2,26⫹/-3,42 mmHg). Conclusion: These preliminary results show a strong blood pressure effect in the II ACE-genotype patients, whereas the reduction of s-creatinine is higher in the DD group. However, the underlying mechanisms have not been clarified yet.
© 2001 by the American Journal of Hypertension, Ltd. Published by Elsevier Science Inc.
Eugene V. Shlayakhto, Anna V. Zucova, Tatyana A. Vinnic, Oleg G. Rudomanov, Yulia B. Nefedova, Irina A. Tolstova, Alexandra O. Conrady. 1Institute of Cardiovascular Diseases, Pavlov State Medical University, St.Petersburg, Russia The aim of the present study was to determine if genetic variants of the renin-angiotensin system genes contribute to the presence of obesity, glucose intolerance, hyperinsulinemia and left ventricular hypertrophy (LVH) in essential hypertensive (EH) patients. We examined 156 patients (the mean age 49⫾8yrs) with mild to moderate EH recruited from the general population of the outpatient hypertensive clinic. Left ventricular mass was measured by echocardiography and left ventricular mass index (LVMI) was estimated. Standard oral glucose tolerance test (OGT) with a parallel measurement of fasting, 30, 60, 90, and 120 min insulin and glucose levels was performed. The areas under the curves (AUC) of glucose and insulin were calculated. Subjects were genotyped for I/D polymorphism of ACE gene, A1166C polymorphism of the AT1 receptor gene, M235T polymorphism of angiotensinogen gene and G/A-6 polymorphism of its promoter region. Genotype distribution of the sample obeyed Hardy-Weinberg equilibrium and was comparable to that reported previously for hypertensive individuals. In groups of patients with different genotype variants the body mass index (BMI), fasting insulin, and AUCs of insulin and glucose were comparable. Neither I/D ACE-gene polymorphism, nor AT1-receptor gene and angiotensinogen gene polymorphisms were associated with LVH. No significant gene-gene interactions contributing to insulin resistance and LVH in EH patients have been found. At the same time, the LVMI level was strongly influenced by BMI (r⫽0.31, p⬍0.01) and fasting insulin level (r⫽0.24, p⬍0.05). In conclusion, we failed to find any association between distinct genotypes of the renin-angiotensin system genes and glucose metabolism as well as with LVH in hypertensive patients. Alternatively, hyperinsulinemia and obesity appears to be important determinants of LVH in EH. Key Words: left ventricular hypertrophy, insulin resistance, genetics
P-136 ANGIOTENSIN II TYPE 2 (AT2) RECEPTOR GENC POLYMORPHISM AND HYPERTENSION IN THE ELDERLY WITH SALTY TASTE DISTURBANCE Shoroku Sakurai, Kohya Okaishi, Shigeto Morimoto. 1Department of Geriatric Medicine, Osaka University Medical School, United States Although hypertension in the elderly is highly dependent on excess intake of sodium, at least partially based on salty taste disturbance, little is known about association of gene polymorphisms with hypertension in the elderly with salty taste disturbance. To clarify the role of gene polymorphisms in this mechanism, 126 elderly female subjects and 61 male subjects were enrolled (mean age ⫾ SD; 81 ⫾ 7 years) for determination of magnitudes of NaCl (1.25%, 2.5%, 5%, 10% and 20%) 0895-7061/01/$20.00
P-133 LACK OF ASSOCIATION OF THE G-PROTEIN SUBUNIT GENE AND LEFT VENTRUCULAR HYPERTROPHY
POSTERS: Genetics/Gene Therapy
II DD ID
75A
CREA 0
Crea 24
⌬ Crea
1,38⫹/- 0,15 1,70⫹/-0,11* 1,33⫹/- 0,15
1,39⫹/-0,28 1,48⫹/-0,16 1,42⫹/-0,16
-0,01⫹/- 0,17 0,23⫹/-0,08* -0,09⫹/- 0,16
Vladimir A. Almasov, Eugene V. Shlyakhto, Eugene I. Shwartz, Anna V. Zucova, Alexandra O. Conrady, Tatyana A. Vinnic. 1Faculty Therapy Department, Pavlov State Medical University, St.Petersburg, Russia
Key Words: antihypertensive drugs, polymorphism, Angiotensine converting enzyme
The aim of the present study was to determine if there is an association of G-protein 3 subunite (GNB3) gene polymorphisms and left ventricular hypertrophy (LVH) in patients with essential hypertension (EH) in St.Petersburg population. We examined 135 patients (the mean age 48⫾7yrs) with mild to moderate EH recruited from the general population of the outpatient hypertensive clinic. Left ventricular mass was measured by echocardiography and left ventricular mass index (LVMI) was calculated. The GNB3 C825T genotype was determined by polymerase chain reaction and restriction digestion. 67 patients (0.50) were homozygous for the C allele (CC), 56 were heterozygous (CT) (0.41) and twelve (0.09) were homozygous for T allele (TT). The distribution of genotypes among the patients was in Hardy-Weinberg equilibrium and did not differ significantly when comparing patients with or without LVH. The frequency of T allele was only slightly higher in patients with LVH (0,32) compared to ones without LVH (0.28) and LVMI was similar in patients with CC, CT and TT genotype (120.2⫾13.4, 120.4⫾13.6, 122.3⫾12.4 g/m2, respectively). In the light of these observations it seems reasonable to conclude that no association of LVH and CNB3 gene variant has been found in the population studied.
P-135 OBESITY, INSULIN RESISTANCE, CARDIAC STRUCTURE, AND RENIN-ANGIOTENSIN SYSTEM GENES IN ESSENTIAL HYPERTENSION
Key Words: Genetics, G-protein, Left ventricular hypertrophy
P-134 DOES ACE POLYMORPHISM INFLUENCE BLOOD PRESSURE AND S-CREATININE UNDER ACEINHIBITOR TREATMENT? Barbara M. Suwelack, Uta Hillebrand, Ulf M.W. Gerhardt, Manuela Kempkes, Karl H. Rahn, Helge Hohage. 1Medizinische Poliklinik, Universita¨t Mu¨nster, Mu¨nster, Germany Aim&Scope: We know 3 different genotypes of the ACE gene, DD, ID and II (I⫽Insertion / D⫽ Deletion). The present study tests the hypothesis that different ACE genotypes may show differences under ACE inhibition. Material and methods: In 3 vs. 4 vs. 9 kidney transplant recipients with genotype II, DD and ID the s-creatinine and the arterial blood pressure were estimated before and after 24 months of ACE inhibitor treatment. Results: The tables show that, unexpectedly, the arterial blood pressure is significantly reduced only in the II group. On the other hand, s-creatinine decreased significantly only in the DD group. (see table) In Group II, the MAD decreased from 114,78⫹/-1,44 mmHg to 93,00⫹/-3,34 mmHg (⌬MAP 21,78⫹/-3,76 mmHg; p⬍0.05). However no significant MAP reduction was found in DD (⌬MAP 0,67⫹/-4,89 mmHg) or ID (⌬ MAP 2,26⫹/-3,42 mmHg). Conclusion: These preliminary results show a strong blood pressure effect in the II ACE-genotype patients, whereas the reduction of s-creatinine is higher in the DD group. However, the underlying mechanisms have not been clarified yet.
© 2001 by the American Journal of Hypertension, Ltd. Published by Elsevier Science Inc.
Eugene V. Shlayakhto, Anna V. Zucova, Tatyana A. Vinnic, Oleg G. Rudomanov, Yulia B. Nefedova, Irina A. Tolstova, Alexandra O. Conrady. 1Institute of Cardiovascular Diseases, Pavlov State Medical University, St.Petersburg, Russia The aim of the present study was to determine if genetic variants of the renin-angiotensin system genes contribute to the presence of obesity, glucose intolerance, hyperinsulinemia and left ventricular hypertrophy (LVH) in essential hypertensive (EH) patients. We examined 156 patients (the mean age 49⫾8yrs) with mild to moderate EH recruited from the general population of the outpatient hypertensive clinic. Left ventricular mass was measured by echocardiography and left ventricular mass index (LVMI) was estimated. Standard oral glucose tolerance test (OGT) with a parallel measurement of fasting, 30, 60, 90, and 120 min insulin and glucose levels was performed. The areas under the curves (AUC) of glucose and insulin were calculated. Subjects were genotyped for I/D polymorphism of ACE gene, A1166C polymorphism of the AT1 receptor gene, M235T polymorphism of angiotensinogen gene and G/A-6 polymorphism of its promoter region. Genotype distribution of the sample obeyed Hardy-Weinberg equilibrium and was comparable to that reported previously for hypertensive individuals. In groups of patients with different genotype variants the body mass index (BMI), fasting insulin, and AUCs of insulin and glucose were comparable. Neither I/D ACE-gene polymorphism, nor AT1-receptor gene and angiotensinogen gene polymorphisms were associated with LVH. No significant gene-gene interactions contributing to insulin resistance and LVH in EH patients have been found. At the same time, the LVMI level was strongly influenced by BMI (r⫽0.31, p⬍0.01) and fasting insulin level (r⫽0.24, p⬍0.05). In conclusion, we failed to find any association between distinct genotypes of the renin-angiotensin system genes and glucose metabolism as well as with LVH in hypertensive patients. Alternatively, hyperinsulinemia and obesity appears to be important determinants of LVH in EH. Key Words: left ventricular hypertrophy, insulin resistance, genetics
P-136 ANGIOTENSIN II TYPE 2 (AT2) RECEPTOR GENC POLYMORPHISM AND HYPERTENSION IN THE ELDERLY WITH SALTY TASTE DISTURBANCE Shoroku Sakurai, Kohya Okaishi, Shigeto Morimoto. 1Department of Geriatric Medicine, Osaka University Medical School, United States Although hypertension in the elderly is highly dependent on excess intake of sodium, at least partially based on salty taste disturbance, little is known about association of gene polymorphisms with hypertension in the elderly with salty taste disturbance. To clarify the role of gene polymorphisms in this mechanism, 126 elderly female subjects and 61 male subjects were enrolled (mean age ⫾ SD; 81 ⫾ 7 years) for determination of magnitudes of NaCl (1.25%, 2.5%, 5%, 10% and 20%) 0895-7061/01/$20.00