- Email: [email protected]
LANGERHANS CELLS IN KAPOSI'S SARCOMA
OOOO Volume 124, Number 2
ABSTRACTS Abstracts e129
CAVALCANTI GALVÃO, MÁRCIA CRISTINA DA COSTA MIGUEL, ROSEANA DE ALMEIDA FREITAS.
PATRÍCIA TEIXEIRA DE OLIVEIRA, ÉRICKA JANINE DANTAS DA SILVEIRA.
This study assessed the frequency of 3 single nucleotide polymorphisms (SNPs) in DNA repair genes RAD51 172g>T (rs1801321), RAD51 135G>C (rs1801320), XRCC3 T241M (rs861539) and investigated its association with clinicopathologic data and outcomes: radio/chemotherapy treatment response, recurrence and overall survival. Samples of 126 cases of oral and oropharyngeal squamous cell carcinoma (OSCC and OPSCC) and 130 control samples were genotyped by real-time PCR. Allelic and genotypic frequencies were in Hard-Weinberg balance. The presence of at least 1 polymorphic allele in XRCC3 gene (rs861539) is associated with higher histologic grade (WHO) (P ¼ .007). It was observed a higher rate of recurrence (P¼.08) and more advanced clinical stage (P¼.08) in the group with at least 1 polymorphic allele in RAD51 (rs1801321). The presence of SNPs associated with smoking or drinking increased from 3 to 150 times the risk of OSCC or OPSCC. It was concluded that the presence of at least 1 polymorphic allele of the SNPs rs861539 in XRCC3 gene, rs1801320 and rs1801321 in RAD51 gene increases the risk of OSCC and OPSCC when associated with the habits of drinking or smoking and that the studied polymorphisms are not associated with treatment response, disease-free survival or overall survival.
It were analyzed 25 cases of pemphigus vulgaris (PV) and 11 cases of benign pemphigoid of mucous membranes (PBMM), in relation to clinical and demographic characteristics, treatment and management. This is a descriptive study, in which the information was collected from the medical records of patients and through a clinical follow-up record. The mean sample age was 41.64 years, female gender was the most frequent (n ¼ 26; 72.22%) and the jugal mucosa the most affected anatomic site (n ¼ 20; 27.40%). Eight patients (22.22%) showed lesions on the skin located in the arms, scalp and perioral region and 7 (19.44%) showed lesions in the genital mucosa, nasal, ocular conjunctiva and pharyngeal. Eight patients (22.22%) were clinically reevaluated, 5 cases of PV and 3 cases of PBMM. Prednisone (systemic 10 to 60 mg/day) associated with Clobetasol propionate (0.05%) (topical) were therapies of choice for 7 patients. The follow-up ranged from 5 months to 5 years. The PV and PBMM are autoimmune lesions which often present the oral cavity as a first clinical manifestation site. Thus, studies investigating clinical features, diagnosis and management are of great importance for better optimization of its treatments.
RP33 - LANGERHANS CELLS IN KAPOSI’S SARCOMA. PABLO AGUSTIN VARGAS, FELIPE PAIVA FONSECA, BELINDA BUNN, WILLIE F.P. VAN HEERDEN. Objective: To evaluate the distribution pattern of Langerhans cells in Kaposi’s sarcoma. Study Design: 51 formalin-fixed, paraffin-embedded cases of Kaposi’s sarcomas were retrospectively retrieved and new 3mm hematoxylin and eosin stained histologic sections were conducted for diagnostic confirmation by 2 oral pathologists. Immunohistochemical reactions against CD83 (mature Langerhans cells) and CD207 (immature Langerhans cells) were carried out and the number of positive cells were counted by one observer in 10 hotspot high-power fields in both of the overlying epithelium and the lesional tissues. Results: During the histopathologic analysis of the studied sample, it was possible to identify different microscopic subtypes of Kaposi’s sarcoma including the solid, telangiectatic, lymphangioma-like, lymphangiectatic, ecchymotic and desmoplastic variants. CD83-positive mature Langerhans cells were predominantly found in the neoplastic tissue than in the overlying epithelium (mean of 37.2 cells VS 8.3 cells, respectively), whereas CD207-positive immature Langerhans cells were predominantly found in the normal epithelium than in the neoplastic tissue (mean of 44.2 cells VS 17.9 cells, respectively). Conclusion: Langerhans cells maturation may be an important process in the development of Kaposi’s sarcoma and its impairment in immunocompromised patients might predispose these individuals to develop the neoplasm.
RP34 - VESICULOBULLOUS AUTOIMMUNE DISEASE WITH ORAL MUCOSA MANIFESTATIONS: RETROSPECTIVE STUDY AND MANAGEMENT OF A SERIE OF CASES. MARA LUANA BATISTA SEVERO, RANI IANI COSTA GONÇALO, ANA MIRYAM COSTA DE MEDEIROS, MÁRCIA CRISTINA DA COSTA MIGUEL, LÉLIA MARIA GUEDES QUEIROZ,
RP35 - MSH2 IMMUNOEXPRESSION IN NORMAL DENTAL GERM, AMELOBLASTOMA AND AMELOBLASTIC CARCINOMA. GLEYSON KLEBER DO AMARAL-SILVA, MANOELA DOMINGUES MARTINS, HÉLDER ANTÔNIO REBELO PONTES, ALAN ROGER SANTOS-SILVA, ANDRÉ CAROLI ROCHA, FELIPE PAIVA FONSECA, PABLO AGUSTIN VARGAS. To determine the expression pattern of MSH2 protein in normal dental germs, ameloblastoma and ameloblastic carcinoma. Three normal dental germs, 12 cases diagnosed as ameloblastoma and 1 case of ameloblastic carcinoma were retrospectively selected from oral pathology archives. New 3mm histologic sections stained on hematoxylin and eosin were conducted for diagnostic confirmation. Immunohistochemical reactions against MSH2 antibody were carried out and the percentage of positive nuclear staining among 1000 total neoplastic cells was obtained for each case in hotspot areas by one observer. Clinical and radiographic data were retrieved from patients’ medical charts. Ameloblastoma cases comprised 4 females and 8 males with a mean age of 36.9 years. It predominantly affected the posterior region of the mandible causing an asymptomatic swelling and leading to multilocular radiolucid images. The only case of ameloblastic carcinoma affected the mandible of a 28-year-old female patient. Regarding MSH2 expression, epithelial component of dental germs revealed 65.5% of positivity, whereas 36.5% and 37.8% of the ameloblastoma and ameloblastic carcinoma cells were positive for this protein, respectively. There is a lower expression of MSH2 in ameloblastoma and ameloblastic carcinoma than in its normal counterpart, what may contribute to the accumulation of DNA mutations and tumor development.
RP36 - M1 AND M2 MACROPHAGES IN POTENTIALLY MALIGNANT DISORDERS AND SQUAMOUS CELL CARCINOMA OF THE ORAL CAVITY AND LIP. JORGE ESQUICHE LEÓN, JESSICA LUANA DOS SANTOS, ALFREDO RIBEIRO DA SILVA, FERNANDO CHAHUD, LANA KEI YAMAMOTO
ABSTRACTS Abstracts e129
CAVALCANTI GALVÃO, MÁRCIA CRISTINA DA COSTA MIGUEL, ROSEANA DE ALMEIDA FREITAS.
PATRÍCIA TEIXEIRA DE OLIVEIRA, ÉRICKA JANINE DANTAS DA SILVEIRA.
This study assessed the frequency of 3 single nucleotide polymorphisms (SNPs) in DNA repair genes RAD51 172g>T (rs1801321), RAD51 135G>C (rs1801320), XRCC3 T241M (rs861539) and investigated its association with clinicopathologic data and outcomes: radio/chemotherapy treatment response, recurrence and overall survival. Samples of 126 cases of oral and oropharyngeal squamous cell carcinoma (OSCC and OPSCC) and 130 control samples were genotyped by real-time PCR. Allelic and genotypic frequencies were in Hard-Weinberg balance. The presence of at least 1 polymorphic allele in XRCC3 gene (rs861539) is associated with higher histologic grade (WHO) (P ¼ .007). It was observed a higher rate of recurrence (P¼.08) and more advanced clinical stage (P¼.08) in the group with at least 1 polymorphic allele in RAD51 (rs1801321). The presence of SNPs associated with smoking or drinking increased from 3 to 150 times the risk of OSCC or OPSCC. It was concluded that the presence of at least 1 polymorphic allele of the SNPs rs861539 in XRCC3 gene, rs1801320 and rs1801321 in RAD51 gene increases the risk of OSCC and OPSCC when associated with the habits of drinking or smoking and that the studied polymorphisms are not associated with treatment response, disease-free survival or overall survival.
It were analyzed 25 cases of pemphigus vulgaris (PV) and 11 cases of benign pemphigoid of mucous membranes (PBMM), in relation to clinical and demographic characteristics, treatment and management. This is a descriptive study, in which the information was collected from the medical records of patients and through a clinical follow-up record. The mean sample age was 41.64 years, female gender was the most frequent (n ¼ 26; 72.22%) and the jugal mucosa the most affected anatomic site (n ¼ 20; 27.40%). Eight patients (22.22%) showed lesions on the skin located in the arms, scalp and perioral region and 7 (19.44%) showed lesions in the genital mucosa, nasal, ocular conjunctiva and pharyngeal. Eight patients (22.22%) were clinically reevaluated, 5 cases of PV and 3 cases of PBMM. Prednisone (systemic 10 to 60 mg/day) associated with Clobetasol propionate (0.05%) (topical) were therapies of choice for 7 patients. The follow-up ranged from 5 months to 5 years. The PV and PBMM are autoimmune lesions which often present the oral cavity as a first clinical manifestation site. Thus, studies investigating clinical features, diagnosis and management are of great importance for better optimization of its treatments.
RP33 - LANGERHANS CELLS IN KAPOSI’S SARCOMA. PABLO AGUSTIN VARGAS, FELIPE PAIVA FONSECA, BELINDA BUNN, WILLIE F.P. VAN HEERDEN. Objective: To evaluate the distribution pattern of Langerhans cells in Kaposi’s sarcoma. Study Design: 51 formalin-fixed, paraffin-embedded cases of Kaposi’s sarcomas were retrospectively retrieved and new 3mm hematoxylin and eosin stained histologic sections were conducted for diagnostic confirmation by 2 oral pathologists. Immunohistochemical reactions against CD83 (mature Langerhans cells) and CD207 (immature Langerhans cells) were carried out and the number of positive cells were counted by one observer in 10 hotspot high-power fields in both of the overlying epithelium and the lesional tissues. Results: During the histopathologic analysis of the studied sample, it was possible to identify different microscopic subtypes of Kaposi’s sarcoma including the solid, telangiectatic, lymphangioma-like, lymphangiectatic, ecchymotic and desmoplastic variants. CD83-positive mature Langerhans cells were predominantly found in the neoplastic tissue than in the overlying epithelium (mean of 37.2 cells VS 8.3 cells, respectively), whereas CD207-positive immature Langerhans cells were predominantly found in the normal epithelium than in the neoplastic tissue (mean of 44.2 cells VS 17.9 cells, respectively). Conclusion: Langerhans cells maturation may be an important process in the development of Kaposi’s sarcoma and its impairment in immunocompromised patients might predispose these individuals to develop the neoplasm.
RP34 - VESICULOBULLOUS AUTOIMMUNE DISEASE WITH ORAL MUCOSA MANIFESTATIONS: RETROSPECTIVE STUDY AND MANAGEMENT OF A SERIE OF CASES. MARA LUANA BATISTA SEVERO, RANI IANI COSTA GONÇALO, ANA MIRYAM COSTA DE MEDEIROS, MÁRCIA CRISTINA DA COSTA MIGUEL, LÉLIA MARIA GUEDES QUEIROZ,
RP35 - MSH2 IMMUNOEXPRESSION IN NORMAL DENTAL GERM, AMELOBLASTOMA AND AMELOBLASTIC CARCINOMA. GLEYSON KLEBER DO AMARAL-SILVA, MANOELA DOMINGUES MARTINS, HÉLDER ANTÔNIO REBELO PONTES, ALAN ROGER SANTOS-SILVA, ANDRÉ CAROLI ROCHA, FELIPE PAIVA FONSECA, PABLO AGUSTIN VARGAS. To determine the expression pattern of MSH2 protein in normal dental germs, ameloblastoma and ameloblastic carcinoma. Three normal dental germs, 12 cases diagnosed as ameloblastoma and 1 case of ameloblastic carcinoma were retrospectively selected from oral pathology archives. New 3mm histologic sections stained on hematoxylin and eosin were conducted for diagnostic confirmation. Immunohistochemical reactions against MSH2 antibody were carried out and the percentage of positive nuclear staining among 1000 total neoplastic cells was obtained for each case in hotspot areas by one observer. Clinical and radiographic data were retrieved from patients’ medical charts. Ameloblastoma cases comprised 4 females and 8 males with a mean age of 36.9 years. It predominantly affected the posterior region of the mandible causing an asymptomatic swelling and leading to multilocular radiolucid images. The only case of ameloblastic carcinoma affected the mandible of a 28-year-old female patient. Regarding MSH2 expression, epithelial component of dental germs revealed 65.5% of positivity, whereas 36.5% and 37.8% of the ameloblastoma and ameloblastic carcinoma cells were positive for this protein, respectively. There is a lower expression of MSH2 in ameloblastoma and ameloblastic carcinoma than in its normal counterpart, what may contribute to the accumulation of DNA mutations and tumor development.
RP36 - M1 AND M2 MACROPHAGES IN POTENTIALLY MALIGNANT DISORDERS AND SQUAMOUS CELL CARCINOMA OF THE ORAL CAVITY AND LIP. JORGE ESQUICHE LEÓN, JESSICA LUANA DOS SANTOS, ALFREDO RIBEIRO DA SILVA, FERNANDO CHAHUD, LANA KEI YAMAMOTO