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Lead phytoremediation: a field application in a Site of National Interest
Special Abstracts / Journal of Biotechnology 150S (2010) S1–S576
also performed. Higher values of specific methanogenic activity (SMA) were in general attained for mesophilic populations incubated with raw SBG (ranging from 1,01 ± 0,08 to 2.75 +0.01Lbiogás / gSSV.d). The presence of a co-substrate seems to enhance SMA results, particularly for thermophilic cultures supplemented with acetate. A higher colour removal in thermophilic (93% ± 2%), than in mesophilic regime (87% ± 4%) was also attained. Results for biodegradation by-products were followed by quantification of COD, protein, acid precipitable polymeric lignin (APPL) and residual soluble lignin (SL). The APPL and protein content in residual liquor depends on the temperature and the pre-treatment of SBG. For instance, for mesophilic cultures incubated with SBG, glucose and dye the APPL content in the residual liquor was 0.1 ± 0.01 g/L and for thermophilic cultures of 0.03 ± 0.04 g/L. The protein content was similar in both regimes, however a very high residual COD was observed for thermophilic cultures (2194 ± 56 mg/L) in comparison with that achieved for mesophilic regime (342 ± 161 mg/L). For pre-washed SBG the APPL content was lower in mesophilic than in thermophilic regime, although a higher residual protein and COD contents were observed. Results indicate that raw SBG is easily degraded in mesophilic regime and pre-washed SBG is more prone to biodegradation under thermophilic conditions. doi:10.1016/j.jbiotec.2010.09.224 [P-E.203] Isolation of hydrocarbon-degrading heavy-metal-resistant bacteria from crude oil-contaminated soil in central chile V. Méndez a,b,c , S. Fuentes a,b,c , M. Hernández a,b,c , V. Morgante a,b,c , M. González a,b,c , M. Seeger a,b,c,∗,1 a
Universidad Técnica Federico Santa María, Chile CCUG - Department of Clinical Bacteriology, Sahlgrenska University Hospital, Sweden c The Sahlgrenska Academy of the University of Gothenburg, Sweden Keywords: hydrocarbon-degrading bacteria; bioremediation; heavy-metal-resistant bacteria; Acinetobacter b
Bioremediation constitutes a promising technology for the clean-up of polluted environments [1]. Aliphatic and aromatic hydrocarbons are main contaminants in oil-polluted environments. Soil contaminated with crude oil was sampled from a polluted site in Valparaíso region, Chile. Hydrocarbon-degrading bacteria were isolated by enrichment in Bushnell-Haas medium with crude oil as sole carbon source and characterized by biochemical and molecular assays. Isolates were identified by 16S rRNA gene sequence analysis as members of Acinetobacter, Pseudomonas and Kocuria genera. The xylE gene encoding catechol 2,3-dioxygenase enzyme of the toluene/xylene degradation pathway was detected in Acinetobacter, Kocuria and Pseudomonas isolates. The nahAc gene encoding the naphthalene dioxygenase large subunit was detected in Acinetobacter calcoaceticus strains. Growth of isolates using aliphatic and aromatic compounds as carbon and energy source was studied. A. radioresistens and A. calcoaceticus strains were able to use n-hexadecane as sole carbon source, and a Pseudomonas stutzeri strain showed growth on naphthalene. Bacterial tolerance to mercury, nickel, lead, cadmium and copper metals was also analysed. A. radioresistens and A. calcoaceticus strains showed resistance to copper and cadmium, which are metals commonly associated with hydrocarbon-polluted sites. The results indicate that Acinetobacter isolates are important hydrocarbon-degrading strains and
1
et al.
S287
are resistant to heavy-metals. In this study, several hydrocarbondegrading bacteria were isolated, and are potential biocatalysts for the clean-up of crude oil-polluted environments. Acknowledgments: CIT NT&SB, Red 12 PBCT, Milenio-P04/007-F (Mideplan, Chile) (MS), USM 130836 and USM 130948 (MS), Västra Götelandsregionen ALFGBG-3238 (EM). SF and MH gratefully acknowledge CONICYT fellowships. Reference Morgante, V., López-López, A., Flores, C., González, M., González, B., Vásquez, M., Rosselló-Mora, R., Seeger, M., 2010. FEMS Microbiol. Ecol 71, 114–126.
doi:10.1016/j.jbiotec.2010.09.225 [P-E.204] Lead phytoremediation: a field application in a Site of National Interest F. Cinelli a,e,∗ , A. Missaglia a,e , L. Toffanin c,e , M. Barbafieri d,e,1
Vettori b,e , C.
Felici c,e , A.
a
University of Pisa DCDSL ‘G.Scaramuzzi’, Italy University of Florence, Italy c University of Pisa DBPA, Italy d CNR ISE, Italy e Ministry of Foreign Affairs IAO, Italy Keywords: Lead; Phytoextraction; Bioremediation; Landscape b
The present work aims to evaluate the applicability of phytoremediation for remediation of lead polluted soil in a Site of National Interest (SIN) that lies in the province of La Spezia, Italy. The research, funded by Italy Penox Company Ltd. provides technical land reclamation as the application of in situ phytoremediation. The project is currently under evaluation by the Ministry for the Environment, Land and Sea to allow a large scale application. In order to identify areas, where contamination exceeds the limits set by law (DM 152/2006) for industrial sites, soil samplings and analyses were carried out. To reduce the impact on the landscape and to limit the agronomic expensive operations relating to future management, native species, including Arbutus unedo L., Hedera elix L., Pinus pinaster Ait., Laurus nobilis L., Smilax aspera L., Ilex aquifolium L., Myrtus communis L., Pteridium aquilinum (L.) Kuhn, Erica arborea L., spread in contaminated areas, were identified by floral and vegetational analyses and their lead content was determined. Trials in container were performed to assess the extraction capacities of plants of Laurus nobilis L. and Myrtus communis L. Positive results were obtained from tests carried out at the end of the plant cultivation. They are evergreen, produce relatively large biomass per production unit and can effectively accumulate Pb compounds from the soil, although with a different behaviour. The results look promising in an increasing proportion of phytoremediation technologies, in order to perform remediation concrete, effective, sustainable and replicable so many of the currently contaminated sites. doi:10.1016/j.jbiotec.2010.09.226
1
et al.
also performed. Higher values of specific methanogenic activity (SMA) were in general attained for mesophilic populations incubated with raw SBG (ranging from 1,01 ± 0,08 to 2.75 +0.01Lbiogás / gSSV.d). The presence of a co-substrate seems to enhance SMA results, particularly for thermophilic cultures supplemented with acetate. A higher colour removal in thermophilic (93% ± 2%), than in mesophilic regime (87% ± 4%) was also attained. Results for biodegradation by-products were followed by quantification of COD, protein, acid precipitable polymeric lignin (APPL) and residual soluble lignin (SL). The APPL and protein content in residual liquor depends on the temperature and the pre-treatment of SBG. For instance, for mesophilic cultures incubated with SBG, glucose and dye the APPL content in the residual liquor was 0.1 ± 0.01 g/L and for thermophilic cultures of 0.03 ± 0.04 g/L. The protein content was similar in both regimes, however a very high residual COD was observed for thermophilic cultures (2194 ± 56 mg/L) in comparison with that achieved for mesophilic regime (342 ± 161 mg/L). For pre-washed SBG the APPL content was lower in mesophilic than in thermophilic regime, although a higher residual protein and COD contents were observed. Results indicate that raw SBG is easily degraded in mesophilic regime and pre-washed SBG is more prone to biodegradation under thermophilic conditions. doi:10.1016/j.jbiotec.2010.09.224 [P-E.203] Isolation of hydrocarbon-degrading heavy-metal-resistant bacteria from crude oil-contaminated soil in central chile V. Méndez a,b,c , S. Fuentes a,b,c , M. Hernández a,b,c , V. Morgante a,b,c , M. González a,b,c , M. Seeger a,b,c,∗,1 a
Universidad Técnica Federico Santa María, Chile CCUG - Department of Clinical Bacteriology, Sahlgrenska University Hospital, Sweden c The Sahlgrenska Academy of the University of Gothenburg, Sweden Keywords: hydrocarbon-degrading bacteria; bioremediation; heavy-metal-resistant bacteria; Acinetobacter b
Bioremediation constitutes a promising technology for the clean-up of polluted environments [1]. Aliphatic and aromatic hydrocarbons are main contaminants in oil-polluted environments. Soil contaminated with crude oil was sampled from a polluted site in Valparaíso region, Chile. Hydrocarbon-degrading bacteria were isolated by enrichment in Bushnell-Haas medium with crude oil as sole carbon source and characterized by biochemical and molecular assays. Isolates were identified by 16S rRNA gene sequence analysis as members of Acinetobacter, Pseudomonas and Kocuria genera. The xylE gene encoding catechol 2,3-dioxygenase enzyme of the toluene/xylene degradation pathway was detected in Acinetobacter, Kocuria and Pseudomonas isolates. The nahAc gene encoding the naphthalene dioxygenase large subunit was detected in Acinetobacter calcoaceticus strains. Growth of isolates using aliphatic and aromatic compounds as carbon and energy source was studied. A. radioresistens and A. calcoaceticus strains were able to use n-hexadecane as sole carbon source, and a Pseudomonas stutzeri strain showed growth on naphthalene. Bacterial tolerance to mercury, nickel, lead, cadmium and copper metals was also analysed. A. radioresistens and A. calcoaceticus strains showed resistance to copper and cadmium, which are metals commonly associated with hydrocarbon-polluted sites. The results indicate that Acinetobacter isolates are important hydrocarbon-degrading strains and
1
et al.
S287
are resistant to heavy-metals. In this study, several hydrocarbondegrading bacteria were isolated, and are potential biocatalysts for the clean-up of crude oil-polluted environments. Acknowledgments: CIT NT&SB, Red 12 PBCT, Milenio-P04/007-F (Mideplan, Chile) (MS), USM 130836 and USM 130948 (MS), Västra Götelandsregionen ALFGBG-3238 (EM). SF and MH gratefully acknowledge CONICYT fellowships. Reference Morgante, V., López-López, A., Flores, C., González, M., González, B., Vásquez, M., Rosselló-Mora, R., Seeger, M., 2010. FEMS Microbiol. Ecol 71, 114–126.
doi:10.1016/j.jbiotec.2010.09.225 [P-E.204] Lead phytoremediation: a field application in a Site of National Interest F. Cinelli a,e,∗ , A. Missaglia a,e , L. Toffanin c,e , M. Barbafieri d,e,1
Vettori b,e , C.
Felici c,e , A.
a
University of Pisa DCDSL ‘G.Scaramuzzi’, Italy University of Florence, Italy c University of Pisa DBPA, Italy d CNR ISE, Italy e Ministry of Foreign Affairs IAO, Italy Keywords: Lead; Phytoextraction; Bioremediation; Landscape b
The present work aims to evaluate the applicability of phytoremediation for remediation of lead polluted soil in a Site of National Interest (SIN) that lies in the province of La Spezia, Italy. The research, funded by Italy Penox Company Ltd. provides technical land reclamation as the application of in situ phytoremediation. The project is currently under evaluation by the Ministry for the Environment, Land and Sea to allow a large scale application. In order to identify areas, where contamination exceeds the limits set by law (DM 152/2006) for industrial sites, soil samplings and analyses were carried out. To reduce the impact on the landscape and to limit the agronomic expensive operations relating to future management, native species, including Arbutus unedo L., Hedera elix L., Pinus pinaster Ait., Laurus nobilis L., Smilax aspera L., Ilex aquifolium L., Myrtus communis L., Pteridium aquilinum (L.) Kuhn, Erica arborea L., spread in contaminated areas, were identified by floral and vegetational analyses and their lead content was determined. Trials in container were performed to assess the extraction capacities of plants of Laurus nobilis L. and Myrtus communis L. Positive results were obtained from tests carried out at the end of the plant cultivation. They are evergreen, produce relatively large biomass per production unit and can effectively accumulate Pb compounds from the soil, although with a different behaviour. The results look promising in an increasing proportion of phytoremediation technologies, in order to perform remediation concrete, effective, sustainable and replicable so many of the currently contaminated sites. doi:10.1016/j.jbiotec.2010.09.226
1
et al.