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Linoleic acid can induce depigmentation in cultured normal human melanocytes during actively proliferating state
P7
P8
STUDY OF HORIZONTAL DISTRIBUTIONS OF LANGERHANS CELLS IN GUINEA PIG EPIDERMIS TOSHIFUMI NAKAGAWA, TOSHIHIKO NUMAHARA AND TAKASHI TAKAIWA Department of Dermatology, Kagawa Medical School. Kagawa The distribution of Langerhans cells(LCs) in the normal epidermis 1s thought t6 be regular. But the regularity did not have been assesed mathematically. Three tvues of distribution are distinguished mathemati&ly, Poisson, aggregate and resular. Some indexes for swtial distribuion have b&n reported. To evaluate iCs distribution, we used two mathematical indexes, Hopkins-Skellam index and Morishita index. The LCs were stained 1" the epidermal sheets by ATPase method. In the normal skin, the horizontal distribution pattern was highly reqular(Pr=0.000001). And sane treated skins, UVB and towical steroid. were studied by the indexes. in the topicai steroid treated skin, the number of LCs was decreased but the regularity was remained. I" the UVB treated skin, the regularity was signifxcantly decreased.
LINOLEIC ACID CAN INDUCE DEPIGMENTATION IN CULTURED NORMAL HUMAN MELANOCYTES DURING ACTIVELY PROLIFERATINGSTATE
A
MATHEMATICAL
HIDEYA ANDO. TATSUYR HORIKAWA,MASAHIRO OKA, MASAMITSU ICHIHASHI, AND YIJTAKAMISHIMA Department of Dermatology,Kobe University School of Medicine, Kobe
?!erecently reported th?: linoleic acid, similar to the PKC activator OAG, can inhibit tyrosinaseactivity and induce depigmentationin cultured B16 melanoma cells (Pigment Cell Res., 3:200-206, 1990). In this study, we further report that similar inhibition can also occur in cultured normal human melanocytes during actively proliferating state through addition of CAMP and bFGF.
PlO RESTRICTION FLAGMENT LENGTH POLYMORPHISM (RPLP) OF
ATSUSHI NAKAZAWA, AICIRAOZAWA. JLINICHISUGAI. MUNEOOHKIW. A!zaKo Ata* HIDBTOSHI 1iaKO* Atul KIMIYGSHI TSUJI'.
GLYCOCONJUGATES IN THE EPIDERMIS OF FAMILIAL BENIGN CHRONIC PEMPHIGUS MASASHI AKIYANAl.*, MAKOTO SUGIURAZ, HIROSHI SHIMI+, AND TAKE.,1 NISHLKAWAl 1Department of Dermatology,Keio Univ. School of Medicine,
Departmentbf Dermatologyand crransplantation II Tokai University School of Medicine, Isehara.
Tokyo, 2Departmentof Dermatolopy,Shimizu City Hospital, Shimizu.
We have already shown that the HLA gene possibly participates in the mechanism of onset of psoriasis. Recent investxqations suggest that various cytokines such as TNF are related to the pathcgenesis of psoriasis. RFLP of the TW gene is also discussed. because both of the HlA and TNF genes are located on the same chromosome. In this study, in order to know the relationship between the BLA and TNF genes in psorisis, rre tried to examine R&P of the TNP gene in 30 psoriasis and 15 controls using Southern blot analysis. TW in serum and PAS1 were also measured in ail patients. Tw allelic bends of 10.5 kb and 5.5 kb xere detected in both patients and controls. But the ratio of combination of these tiso bands in psoriasis were obviously different from controls &X0.05). We did not find any correlation between these band pattems,tbe HWL-B and C antigens, PASI. and quantity of TNF. respectively. These results suggested as follows. (1) The production of TN? does not directly participate in the onset of the disease. (2) The disease susceptible gene of psoriasis should IX located around the I&-C gene on the sixth chromosome.
In order to reveal the distribution of glycoconjupates in the epidermis of familial benign chronic pemphigus (FBCP), we applied lectin histochemical techniques to skin biopsy The biopsy specimens were specimens of 7 cases of FBCP. fixed in 10% paraformaldehgde and embedded in paraffin. 4pm thick serial sections were used for lectin srain. After trypsin treatment and inhibition of endogenous peroxidase activity, lectin stainings were done using The leccins employed in avidin-biotin complex methods. this study were as follows: Concanavalin A, Dolichos biflors agglutinin, peanut agglutinin, Ricin.u. commllnis agglutinin I and II, soybean agglutinin, and wheat germ For the control specimens,normal skin and agglutinin. ski" biopsy materials from pemphigus vulgaris were Specific binding patterns of the lectins were employed. observed in the epidermis of FBCP.
PI1
P12
THE TLIMOR NECROSIS FACTOR (TNF) GENE IN PSORIASIS VLIMXIIS.
THE
EFFECT
RESERVOIR
HIROKO
OF
FUNCTIONS
MIYA”CHI,
Department
ULTA”IclLET
LIGHT
IRRADIATION
OF THE STRATUM
TAKESHI
HORIO
AND
ULTRAVIOLET
ON WATER-
CORNBUM
ADHESION
YASUO
FUKUMl
ASADA
of Dermatology,~ansal Medical University,
B
LIGHT
FURUKAWAKEN-ICHI
IRRADIATION
TODA
AUGhTEmS
AND
SADAO
KEMTINOCYTES
IMAMURA
Department of Dermatology. Facultyof Medicine. Kyoto Umversity
Moriyuchi The stratum corneum plays an important role to keep the skin surface supple and flex&Ie. After the exposure to sunlight, the skin may become dry and scaly. In the present study, the water content,hygroscopicity and waterholding capacity of the stratum corneum were examined after UVB exposure to the guinea pig skin. Manually depilated
Uitravioiet (UV) B hghf iriadmhonis wellknown to inducecyiohinesfrom cultured keratmofytesand alsoto augment cellproliferatwn. In th,sstudy.we mvesflgatedthe associahonbetween UVB irradiation and celll adhesion. Culturedkeratinocytes (EPL- PAK) were maintainedin KGM
medium (O.lmM
Ca'-). Cellswere rradiatedwith UVB lightat the doses of 0. IO.20.50. 100 and ZXmJ/cm'. Twenty-four hours afterthe tiradiatmn. the number of cells
back skin was exposed to 1.2 and 3 MEDs of UVB, and a timecourse study was performed.the decreasedwater content and water-holdingcapacity was noted an day 1, and persisted until day 10 to 14. In contrast, a decrease in hygroscoplclty appeared 3 days after exposure and returned to
detachedby 0.05% trypsinsolutmnwas countedevery 10 mmutes. Low doses of UVB
(IO or ZOml/cm*) augmented cellattachmentratio. Cellattachment
assay revealedthat cellsirradiated with low dose UVB
preirradlated state on day 7. The impaired functional parameters were pertially prevented by topical application of sunscreening agent. These results indicate that a single exposure to UVB can damage the functions of stratum corneum.
248
hght had hxher
P8
STUDY OF HORIZONTAL DISTRIBUTIONS OF LANGERHANS CELLS IN GUINEA PIG EPIDERMIS TOSHIFUMI NAKAGAWA, TOSHIHIKO NUMAHARA AND TAKASHI TAKAIWA Department of Dermatology, Kagawa Medical School. Kagawa The distribution of Langerhans cells(LCs) in the normal epidermis 1s thought t6 be regular. But the regularity did not have been assesed mathematically. Three tvues of distribution are distinguished mathemati&ly, Poisson, aggregate and resular. Some indexes for swtial distribuion have b&n reported. To evaluate iCs distribution, we used two mathematical indexes, Hopkins-Skellam index and Morishita index. The LCs were stained 1" the epidermal sheets by ATPase method. In the normal skin, the horizontal distribution pattern was highly reqular(Pr=0.000001). And sane treated skins, UVB and towical steroid. were studied by the indexes. in the topicai steroid treated skin, the number of LCs was decreased but the regularity was remained. I" the UVB treated skin, the regularity was signifxcantly decreased.
LINOLEIC ACID CAN INDUCE DEPIGMENTATION IN CULTURED NORMAL HUMAN MELANOCYTES DURING ACTIVELY PROLIFERATINGSTATE
A
MATHEMATICAL
HIDEYA ANDO. TATSUYR HORIKAWA,MASAHIRO OKA, MASAMITSU ICHIHASHI, AND YIJTAKAMISHIMA Department of Dermatology,Kobe University School of Medicine, Kobe
?!erecently reported th?: linoleic acid, similar to the PKC activator OAG, can inhibit tyrosinaseactivity and induce depigmentationin cultured B16 melanoma cells (Pigment Cell Res., 3:200-206, 1990). In this study, we further report that similar inhibition can also occur in cultured normal human melanocytes during actively proliferating state through addition of CAMP and bFGF.
PlO RESTRICTION FLAGMENT LENGTH POLYMORPHISM (RPLP) OF
ATSUSHI NAKAZAWA, AICIRAOZAWA. JLINICHISUGAI. MUNEOOHKIW. A!zaKo Ata* HIDBTOSHI 1iaKO* Atul KIMIYGSHI TSUJI'.
GLYCOCONJUGATES IN THE EPIDERMIS OF FAMILIAL BENIGN CHRONIC PEMPHIGUS MASASHI AKIYANAl.*, MAKOTO SUGIURAZ, HIROSHI SHIMI+, AND TAKE.,1 NISHLKAWAl 1Department of Dermatology,Keio Univ. School of Medicine,
Departmentbf Dermatologyand crransplantation II Tokai University School of Medicine, Isehara.
Tokyo, 2Departmentof Dermatolopy,Shimizu City Hospital, Shimizu.
We have already shown that the HLA gene possibly participates in the mechanism of onset of psoriasis. Recent investxqations suggest that various cytokines such as TNF are related to the pathcgenesis of psoriasis. RFLP of the TW gene is also discussed. because both of the HlA and TNF genes are located on the same chromosome. In this study, in order to know the relationship between the BLA and TNF genes in psorisis, rre tried to examine R&P of the TNP gene in 30 psoriasis and 15 controls using Southern blot analysis. TW in serum and PAS1 were also measured in ail patients. Tw allelic bends of 10.5 kb and 5.5 kb xere detected in both patients and controls. But the ratio of combination of these tiso bands in psoriasis were obviously different from controls &X0.05). We did not find any correlation between these band pattems,tbe HWL-B and C antigens, PASI. and quantity of TNF. respectively. These results suggested as follows. (1) The production of TN? does not directly participate in the onset of the disease. (2) The disease susceptible gene of psoriasis should IX located around the I&-C gene on the sixth chromosome.
In order to reveal the distribution of glycoconjupates in the epidermis of familial benign chronic pemphigus (FBCP), we applied lectin histochemical techniques to skin biopsy The biopsy specimens were specimens of 7 cases of FBCP. fixed in 10% paraformaldehgde and embedded in paraffin. 4pm thick serial sections were used for lectin srain. After trypsin treatment and inhibition of endogenous peroxidase activity, lectin stainings were done using The leccins employed in avidin-biotin complex methods. this study were as follows: Concanavalin A, Dolichos biflors agglutinin, peanut agglutinin, Ricin.u. commllnis agglutinin I and II, soybean agglutinin, and wheat germ For the control specimens,normal skin and agglutinin. ski" biopsy materials from pemphigus vulgaris were Specific binding patterns of the lectins were employed. observed in the epidermis of FBCP.
PI1
P12
THE TLIMOR NECROSIS FACTOR (TNF) GENE IN PSORIASIS VLIMXIIS.
THE
EFFECT
RESERVOIR
HIROKO
OF
FUNCTIONS
MIYA”CHI,
Department
ULTA”IclLET
LIGHT
IRRADIATION
OF THE STRATUM
TAKESHI
HORIO
AND
ULTRAVIOLET
ON WATER-
CORNBUM
ADHESION
YASUO
FUKUMl
ASADA
of Dermatology,~ansal Medical University,
B
LIGHT
FURUKAWAKEN-ICHI
IRRADIATION
TODA
AUGhTEmS
AND
SADAO
KEMTINOCYTES
IMAMURA
Department of Dermatology. Facultyof Medicine. Kyoto Umversity
Moriyuchi The stratum corneum plays an important role to keep the skin surface supple and flex&Ie. After the exposure to sunlight, the skin may become dry and scaly. In the present study, the water content,hygroscopicity and waterholding capacity of the stratum corneum were examined after UVB exposure to the guinea pig skin. Manually depilated
Uitravioiet (UV) B hghf iriadmhonis wellknown to inducecyiohinesfrom cultured keratmofytesand alsoto augment cellproliferatwn. In th,sstudy.we mvesflgatedthe associahonbetween UVB irradiation and celll adhesion. Culturedkeratinocytes (EPL- PAK) were maintainedin KGM
medium (O.lmM
Ca'-). Cellswere rradiatedwith UVB lightat the doses of 0. IO.20.50. 100 and ZXmJ/cm'. Twenty-four hours afterthe tiradiatmn. the number of cells
back skin was exposed to 1.2 and 3 MEDs of UVB, and a timecourse study was performed.the decreasedwater content and water-holdingcapacity was noted an day 1, and persisted until day 10 to 14. In contrast, a decrease in hygroscoplclty appeared 3 days after exposure and returned to
detachedby 0.05% trypsinsolutmnwas countedevery 10 mmutes. Low doses of UVB
(IO or ZOml/cm*) augmented cellattachmentratio. Cellattachment
assay revealedthat cellsirradiated with low dose UVB
preirradlated state on day 7. The impaired functional parameters were pertially prevented by topical application of sunscreening agent. These results indicate that a single exposure to UVB can damage the functions of stratum corneum.
248
hght had hxher