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Lip squamous cell carcinoma mimicking keratoacanthoma
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e82
Abstracts
After six months of follow-up there was no sign of recurrence.
LIP SQUAMOUS CELL CARCINOMA MIMICKING KERATOACANTHOMA Roberto1,2,3,4,5,6,7 - 1FOAR UNESP - FISIOLOGIA E PATOLOGIA, 2MARIO FRANCISCO REAL GABRIELLI - DIAGNÓSTICO E CIRURGIA, 3 ELAINE MARIA SGAVIOLI MASSUCATO - DIAGNÓSTICO E CIRURGIA, 4MIRIAN APARECIDA ONOFRE - DIAGNÓSTICO E CIRURGIA, 5PAULO FERMINO DA COSTA NETO - GRADUANDO (FOAR), 6VITOR AUGUSTO LEITE - RESIDENTE CIRURGIA, 7EWERSON RAPHAEL WATANABE - RESIDENTE CIRURGIA Squamous cell carcinoma, the most common type of lip cancer (95%), is initially asymptomatic and manifests as leukoplakic or erythroplakic atrophic plaques, crusty indurated or ulcerated lesion. Keratoacanthoma is a rapid growth, followed by slow, spontaneous involution over several months and the mature lesion is usually bud or dome-shaped and it is brownish or slightly reddish. MIDV, a 63 year-old white female was referred to the Department of Diagnosis and Surgery of FOAR-Unesp for treatment of a lesion in the lower lip. She had a long history of exposure to solar radiation. This was a 1.5 cm diameter lesion of rapid growth, being first noticed by the patient 60 days previously. It was exophytic, containing a central keratin-filled crater. The patient also referred spontaneous pain and discomfort. The clinical diagnosis was Keratoacanthoma, but incisional biopsy revealed squamous cell carcinoma. Treatment was surgical resection with safety margins. Financial support: Univ Estadual Paulista, Fundunesp (00472/12-DFP).
IMMUNOEXPRESSION OF GLUCOSE TRANSPORTERS 1 AND 3 IN LOWER LIP SQUAMOUS CELL CARCINOMA AND ITS RELATIONSHIP WITH CLINICOPATHOLOGICAL PARAMETERS Carvalho CHP2,1, Demeda CF1, Aquino ARL1,3, Nonaka CFW4, Souza LB1, Pereira Pinto L1 - 1UFRN - ODONTOLOGIA, 2UFCG - UACB, 3UERN ODONTOLOGIA, 4UEPB - ODONTOLOGIA Objective: This study evaluated, through immunohistochemistry, the expression of glucose transporters (GLUTs) 1 and 3 in the lower lip squamous cell carcinoma (LLSCC). Study design: The percentages of immunostained were evaluated in 20 cases of LLSCC with regional nodal metastasis and 20 LLSCC without metastasis. Results: There was a moderate positive correlation between cytoplasmic/membranous and nuclear immunoexpressions of GLUT 1 in invasion front and in the tumor center (p ⬍0.001). For GLUT 3, was also observed a moderate statistically significant positive correlation between cytoplasmic/membranous expression in tumor invasion front and in tumor center (p ⬍0.001). The immunoreactivity for GLUT 1 was higher than GLUT 3 in invasion front (p ⬍0.001) and tumor center (p ⬍0.001). Conclusions: This study suggests that tumor hypoxia is a remarkable characteristic of the LLSCC and GLUT 1 may be responsible for glucose uptake into the interior of the malignant cells.
APICAL MAXILLARY CYST ASSOCIATED WITH ACTINOMYCES: CASE REPORT Sierra A, Segovia D1, Troncoso P2, Bustos M3, Lobos N4, Valenzuela P1, Lisboa D - 1DR.
October 2012 FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF DENTISTRY AND MAXILLOFACIAL SURGERY, 2DR. FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF PATHOLOGICAL ANATOMY, 3DR. FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF INFECTOLOGY, 4SCHOOL OF DENTRISTRY, UNIVERSITY OF CHILE - ASSOCIATE PROFESSOR IREPO Actinomyces species, a gram-positive anaerobic filamentous bacteria, have been related to persistent periapical lesions presenting tissue destruction, osteolysis and tumor-like shape. Diagnosis should be based mainly on clinical history and the identification of “sulfur granules” during macroscopic examination of the biopsied specimen. We present a 33-years-old female, history of facial trauma during adolescence, who referred to our department with a right maxillar swelling in relation to a persistent oral sinus tract at tooth 1.2 endodontically treated, complaining of effervescence-like sensation inside the maxillary bone and posterior nasal discharge. Images show an apical radiolucent circumscribed lesion and cortical involvement of the maxillary sinus floor. Total enucleation of the lesion was performed and then sent to histopathologic study, which reported “apical cyst with presence of actinomyces”. A 4 weeks high-doses antibiotics protocol achieved remission of acute symptoms. Chronic evolution of the disease is currently controlled and treated by a longterm maintenance dose.
FASN INHIBITION AFFECTS THE PROLIFERATION AND MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS IN THREE-DIMENSIONAL CULTURES Bastos DC1, Paupert J, Maillard C2, Seguin F1, Agostini M1, Nöel A2, Graner E1 - 1SCHOOL OF DENTISTRY OF PIRACICABA, UNIVERSITY OF CAMPINAS (UNICAMP) - DEPARTMENT OF ORAL DIAGNOSIS, 2UNIVERSITY OF LIÈGE - GROUPE INTERDISCIPLINAIRE DE GÉNOPROTÉOMIQUE APPLIQUÉ-CANCER (GIGACANCER) Background: FASN enzyme is essential for lipogenesis in tumor cells. Human malignancies, such as melanoma, exhibit elevated FASN levels. We have previously shown that FASN inhibition with orlistat reduces the number of mediastinal lymph node metastasis following the implantation of B16-F10 mouse melanoma cells in the peritoneal cavity of C57BL/6 mice. Objective: This work aimed to investigate the effects of FASN inhibitors in lymphangiogenesis in three-dimensional cell cultures. Study design: For the in vitro assay, HDLEC-hTERT cells were seeded in a methylcellulose solution to form spheroids which were treated with orlistat or cerulenin. For the ex vivo assay, the lymphatic duct of C57BL6 mice was removed and the explants cultivated under hypoxic conditions in the presence of orlistat or cerulenin. Images were acquired and analyzed with the aid of the ImageJ program. Results: FASN inhibition with cerulenin or orlistat reduced the proliferation and/or migration of lymphatic endothelial cells in ‘lymphatic ring assay’. Lymphatic cell branching in the spheroid in vitro assay with conditioned medium from both ceruleninand orlistat-treated B16-F10 cells was also reduced when compared to the controls. Conclusion: These results suggest that FASN inhibitors affect the cell migration and/or proliferation in three-dimensional lymphatic cell cultures.
e82
Abstracts
After six months of follow-up there was no sign of recurrence.
LIP SQUAMOUS CELL CARCINOMA MIMICKING KERATOACANTHOMA Roberto1,2,3,4,5,6,7 - 1FOAR UNESP - FISIOLOGIA E PATOLOGIA, 2MARIO FRANCISCO REAL GABRIELLI - DIAGNÓSTICO E CIRURGIA, 3 ELAINE MARIA SGAVIOLI MASSUCATO - DIAGNÓSTICO E CIRURGIA, 4MIRIAN APARECIDA ONOFRE - DIAGNÓSTICO E CIRURGIA, 5PAULO FERMINO DA COSTA NETO - GRADUANDO (FOAR), 6VITOR AUGUSTO LEITE - RESIDENTE CIRURGIA, 7EWERSON RAPHAEL WATANABE - RESIDENTE CIRURGIA Squamous cell carcinoma, the most common type of lip cancer (95%), is initially asymptomatic and manifests as leukoplakic or erythroplakic atrophic plaques, crusty indurated or ulcerated lesion. Keratoacanthoma is a rapid growth, followed by slow, spontaneous involution over several months and the mature lesion is usually bud or dome-shaped and it is brownish or slightly reddish. MIDV, a 63 year-old white female was referred to the Department of Diagnosis and Surgery of FOAR-Unesp for treatment of a lesion in the lower lip. She had a long history of exposure to solar radiation. This was a 1.5 cm diameter lesion of rapid growth, being first noticed by the patient 60 days previously. It was exophytic, containing a central keratin-filled crater. The patient also referred spontaneous pain and discomfort. The clinical diagnosis was Keratoacanthoma, but incisional biopsy revealed squamous cell carcinoma. Treatment was surgical resection with safety margins. Financial support: Univ Estadual Paulista, Fundunesp (00472/12-DFP).
IMMUNOEXPRESSION OF GLUCOSE TRANSPORTERS 1 AND 3 IN LOWER LIP SQUAMOUS CELL CARCINOMA AND ITS RELATIONSHIP WITH CLINICOPATHOLOGICAL PARAMETERS Carvalho CHP2,1, Demeda CF1, Aquino ARL1,3, Nonaka CFW4, Souza LB1, Pereira Pinto L1 - 1UFRN - ODONTOLOGIA, 2UFCG - UACB, 3UERN ODONTOLOGIA, 4UEPB - ODONTOLOGIA Objective: This study evaluated, through immunohistochemistry, the expression of glucose transporters (GLUTs) 1 and 3 in the lower lip squamous cell carcinoma (LLSCC). Study design: The percentages of immunostained were evaluated in 20 cases of LLSCC with regional nodal metastasis and 20 LLSCC without metastasis. Results: There was a moderate positive correlation between cytoplasmic/membranous and nuclear immunoexpressions of GLUT 1 in invasion front and in the tumor center (p ⬍0.001). For GLUT 3, was also observed a moderate statistically significant positive correlation between cytoplasmic/membranous expression in tumor invasion front and in tumor center (p ⬍0.001). The immunoreactivity for GLUT 1 was higher than GLUT 3 in invasion front (p ⬍0.001) and tumor center (p ⬍0.001). Conclusions: This study suggests that tumor hypoxia is a remarkable characteristic of the LLSCC and GLUT 1 may be responsible for glucose uptake into the interior of the malignant cells.
APICAL MAXILLARY CYST ASSOCIATED WITH ACTINOMYCES: CASE REPORT Sierra A, Segovia D1, Troncoso P2, Bustos M3, Lobos N4, Valenzuela P1, Lisboa D - 1DR.
October 2012 FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF DENTISTRY AND MAXILLOFACIAL SURGERY, 2DR. FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF PATHOLOGICAL ANATOMY, 3DR. FELIX BULNES CLINICAL HOSPITAL - DEPARTMENT OF INFECTOLOGY, 4SCHOOL OF DENTRISTRY, UNIVERSITY OF CHILE - ASSOCIATE PROFESSOR IREPO Actinomyces species, a gram-positive anaerobic filamentous bacteria, have been related to persistent periapical lesions presenting tissue destruction, osteolysis and tumor-like shape. Diagnosis should be based mainly on clinical history and the identification of “sulfur granules” during macroscopic examination of the biopsied specimen. We present a 33-years-old female, history of facial trauma during adolescence, who referred to our department with a right maxillar swelling in relation to a persistent oral sinus tract at tooth 1.2 endodontically treated, complaining of effervescence-like sensation inside the maxillary bone and posterior nasal discharge. Images show an apical radiolucent circumscribed lesion and cortical involvement of the maxillary sinus floor. Total enucleation of the lesion was performed and then sent to histopathologic study, which reported “apical cyst with presence of actinomyces”. A 4 weeks high-doses antibiotics protocol achieved remission of acute symptoms. Chronic evolution of the disease is currently controlled and treated by a longterm maintenance dose.
FASN INHIBITION AFFECTS THE PROLIFERATION AND MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS IN THREE-DIMENSIONAL CULTURES Bastos DC1, Paupert J, Maillard C2, Seguin F1, Agostini M1, Nöel A2, Graner E1 - 1SCHOOL OF DENTISTRY OF PIRACICABA, UNIVERSITY OF CAMPINAS (UNICAMP) - DEPARTMENT OF ORAL DIAGNOSIS, 2UNIVERSITY OF LIÈGE - GROUPE INTERDISCIPLINAIRE DE GÉNOPROTÉOMIQUE APPLIQUÉ-CANCER (GIGACANCER) Background: FASN enzyme is essential for lipogenesis in tumor cells. Human malignancies, such as melanoma, exhibit elevated FASN levels. We have previously shown that FASN inhibition with orlistat reduces the number of mediastinal lymph node metastasis following the implantation of B16-F10 mouse melanoma cells in the peritoneal cavity of C57BL/6 mice. Objective: This work aimed to investigate the effects of FASN inhibitors in lymphangiogenesis in three-dimensional cell cultures. Study design: For the in vitro assay, HDLEC-hTERT cells were seeded in a methylcellulose solution to form spheroids which were treated with orlistat or cerulenin. For the ex vivo assay, the lymphatic duct of C57BL6 mice was removed and the explants cultivated under hypoxic conditions in the presence of orlistat or cerulenin. Images were acquired and analyzed with the aid of the ImageJ program. Results: FASN inhibition with cerulenin or orlistat reduced the proliferation and/or migration of lymphatic endothelial cells in ‘lymphatic ring assay’. Lymphatic cell branching in the spheroid in vitro assay with conditioned medium from both ceruleninand orlistat-treated B16-F10 cells was also reduced when compared to the controls. Conclusion: These results suggest that FASN inhibitors affect the cell migration and/or proliferation in three-dimensional lymphatic cell cultures.