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Lipidic profile and insulin sensitivity in women with PCOS
301 LIPIDIC PROFILE AND INSULIN SENSITIVITY IN WOMEN WITH PCOS
XVIII S.I.S.A. National Congress
AMATO MC, MERLINO S, GIORDANO G, GALLUZZO A
PPARy AND CD-36 RECEPTOR EXPRESSION IN MONOCYTE/MACROPHAGES OF HEALTHY SMOKERS AND NONSMOKERS
Endocrinology and Metabolic Diseases. Universita di Palermo
Amoruso A., Viano I., Brunelleschi S.
The Policystic Ovaric Syndrome (PCOS) is well-known associated with a lypidic profile characterized by hypertriglyceridemia, low levels of HDLcholesterol and high levels of small and dense LDL A group of 97 patients, without previous diagnosis of diabetes and dislypidemia, with an average age of 24 years-old, suffering from hirsutism, oligomenorrhea, acne-seborrhea, were hospitalized in our Day Hospital. The patients were screened for OGTT, lypidic profile, insulin sensitivity (Homa IR, Matsuda index), and hypothalamus-pituitary-ovary axis. The evaluation of the lypidic profile, according to ATP III guide lines, showed that 22,5% of the patients had cholesteroI-LDL level beetwen 130 and 159 mg/dl; 11,3% beetwen 160 and 189 mg/dl; 3,8% more than 190 mg/dl. We observed an inverse correlation beetwen HDL-cholesterol and triglycerides, BMI, Ferriman and Gallwey index, waist measure, and all the insulin resistance indexes (p<0,01). Besides, we observed a lower HDL-cholesterol level in hirsute patients when compared to not-hirsute ones (49,10 -+ 10,88 vs 62,22 + 17,12 mg/dl). Briefly, 37.6% of examinated young women had LDL-cholesterol level higher than standard, and this is not related with insulin sensitivity. Thus, we suggest to screen for dislypidemia patients, with PCOS, in which we usually expect a typical lypidic profile, characterized by hyper triglyceridemia and low HDL-cholesterol levels (strongly influenced by insulin-resistance degree, mostly in hirsute patients), but not a high cholesteroI-LDL level.
Dept. Medical Sciences, School of Medicine, University of Piemonte Orientale "A. Avogadro", Via Solaroli, 1 7 - 28100 NOVARA
Metabolic syndrome and cardiovascular risk: data from a large dyslipidemic population
ANTIOXIDANT EFFECTIVENESS OF SICILIAN RED WINES IN COMPARISON WITH FRENCH RED WINES
Giusto L, Vigna GB, Tosini E, Bergami E, Fellin R.
D. Di Majo, M. Giammanco, E. Tripoli, P. Gagliano, G. Tabacchi, S. Giammanco, M. La Guardia
Sect. Internal Medicine, Gerontol. Geriatry, Dept. Clin. and Exper. Med., University of Ferrara We evaluated all subjects addressing our Metabolic Center in a two-year period, looking for metabolic syndrome prevalence and cardiovascular disease association. Different definitions of the metabolic syndrome were considered: all appeared highly correlated. At last we categorized patients according to overweight (BMI>25 Kg/m2 in women and >30 in men), abnormal glucose level ~110 mg/dl)or diabetes, high blood pressure (~130/=85 mmHg) and a unified-dyslipidemic parameter (triglycerides a150 mg/dl and/or HDL-C <40 mg/dl in men and/or <50 mg/dl in women). This definition may be considered a variant of ATP-III proposal and 22.6% of all examined patients (n=1124, most of whom hypercholesterolemic) were recognized as carrier of at least 3 metabolic cdteria, while 4.5% had a full-expressed syndrome (4 criteria). The prevalence of subjects (particularly men) with metabolic syndrome (3 or 4 criteria) and documented coronary heart disease (CHD) was more than twice that of remaining patients (12.6% vs. 5.9%, p<0.001). Possible CHD (poor or incomplete clinical evidence) was similarly more prevalent among 'metabolic' cases than others (3.9% vs. 1.6%, p<0.01). Ill-defined CHD was more frequently found in women than in men (4.8% vs. 2.3%). These data have been confirmed also after stratification by age ~65 and >65 years). Peripheral arterial disease was 1.5-2 times more prevalent in patients with the metabolic syndrome than in other subjects, and this result was more significant in younger people ~65 years). We conclude that the metabolic cluster is associated with increased arterial disease also in this setting of high-risk dyslipidemic patients.
Monocyte/macrophages are known to play a relevant role in the development of atherosclerosis. Peroxisome proliferator-activated receptor-y(PPAR-~;) is an important regulator of genes controlling lipid and glucose metabolism, which is activated by naturally occurring ligands, including 15-deoxy-PGJ2 (15d-PGJ2) and ox-LDL, as well as by synthetic agents, such as the thiazolidinedione antidiabetics. Experimental evidences suggest that PPAR-y; can exert a relevant role in atherosclerosis. We investigated the expression of PPAR-y and CD-36 receptors in monocyte/macrophages of healthy smokers and nonsmokers. Monocytes (M) were collected from heparinized venous blood of healthy volunteers by standard techniques and purified by adhesion; monocyte-derived macrophages (MDM) were prepared from M cultured for 8 days in RPMI 1640 medium enriched with 20% FCS. PPAR-y and CD-36 expressions were evaluated by Immunoblotting and Flow cytometry, respectively. The basal expression of PPAR-y; is minimal in M (ratio PPAR-?;/6actin = 0.23 + 0.04; n=9), higher after 4 days of differentiation (0.33 + 0.03; n=9) and very relevant in MDM (0.55 + 0.04; n=9; p < 0.01). The expression of PPAR-y; in M and MDM is increased when the cells are incubated with 15d-PGJ2 (5 I~M, 6h) or ciglitazone (50 ~_M, 6h). Preliminary data indicate that the basal expression of PPAR-y; in healthy smokers is 2.5-3 fold higher than in non-smokers. Expression of CD-36 on M and MDM is about 50-60% in non-smokers and 70-80% in smokers. These results indicate that PPAR-y; is expressed in M and MDM and suggest a possible role in atherosclerosis.
Istituto di Fisiologia e Nutrizione Umana, Universit~ di Palermo, via Augusto Ella,3 -90127- Palermo Introduction: Coronary artery disease (CAD) is the most dangerous disease of the second half of the XX century. The scientific community has proposed some measures for reducing the high level of mortality from CAD.The pathogenetic basis of CAD is first of all the high level of cholesterol in blood.Recent studies suggest that one of the most important mechanisms predisposing to development of atherosclerosis is the oxidation of LDL-C particles. It has been demonstrated both in vitro and in vivo that the phenolic compounds can offer significant antiatherogenic protection by inhibiting the oxidation of LDL. Aim: Purpose of this work was to evaluate the antioxidant effectiveness and the phenolic composition of Sicilian red wines in comparison with French red wines. Methods: we analysed 24 red wines (11 Sicilian and 13 French). Total phenols were analysed according to the Folin-Ciocalteau method. Antioxidant capacity was determined by a method based on crocin bleaching by oxidation due to a source of radicals. Statistical data analysis was performed by the ANOVA test. Results: The concentration of total phenol varied from 3578 mg/L GAE to 2370 mg/L GAE for Sicilian red wines while in the French red wines is inclusive from 3100 mg/L GAE to 1533 mg/LGAE. The Sicilian red wines studied have shown higher levels of antioxidant capacity than the French wines. Conclusion: This study show that the amounts of phenols in Sicilian red wines is most abundant when compared to the other French samples. Moreover, this study evidences that the antioxidant activity is correlated with total phenol content of wine.(POSTER)
XVIII S.I.S.A. National Congress
AMATO MC, MERLINO S, GIORDANO G, GALLUZZO A
PPARy AND CD-36 RECEPTOR EXPRESSION IN MONOCYTE/MACROPHAGES OF HEALTHY SMOKERS AND NONSMOKERS
Endocrinology and Metabolic Diseases. Universita di Palermo
Amoruso A., Viano I., Brunelleschi S.
The Policystic Ovaric Syndrome (PCOS) is well-known associated with a lypidic profile characterized by hypertriglyceridemia, low levels of HDLcholesterol and high levels of small and dense LDL A group of 97 patients, without previous diagnosis of diabetes and dislypidemia, with an average age of 24 years-old, suffering from hirsutism, oligomenorrhea, acne-seborrhea, were hospitalized in our Day Hospital. The patients were screened for OGTT, lypidic profile, insulin sensitivity (Homa IR, Matsuda index), and hypothalamus-pituitary-ovary axis. The evaluation of the lypidic profile, according to ATP III guide lines, showed that 22,5% of the patients had cholesteroI-LDL level beetwen 130 and 159 mg/dl; 11,3% beetwen 160 and 189 mg/dl; 3,8% more than 190 mg/dl. We observed an inverse correlation beetwen HDL-cholesterol and triglycerides, BMI, Ferriman and Gallwey index, waist measure, and all the insulin resistance indexes (p<0,01). Besides, we observed a lower HDL-cholesterol level in hirsute patients when compared to not-hirsute ones (49,10 -+ 10,88 vs 62,22 + 17,12 mg/dl). Briefly, 37.6% of examinated young women had LDL-cholesterol level higher than standard, and this is not related with insulin sensitivity. Thus, we suggest to screen for dislypidemia patients, with PCOS, in which we usually expect a typical lypidic profile, characterized by hyper triglyceridemia and low HDL-cholesterol levels (strongly influenced by insulin-resistance degree, mostly in hirsute patients), but not a high cholesteroI-LDL level.
Dept. Medical Sciences, School of Medicine, University of Piemonte Orientale "A. Avogadro", Via Solaroli, 1 7 - 28100 NOVARA
Metabolic syndrome and cardiovascular risk: data from a large dyslipidemic population
ANTIOXIDANT EFFECTIVENESS OF SICILIAN RED WINES IN COMPARISON WITH FRENCH RED WINES
Giusto L, Vigna GB, Tosini E, Bergami E, Fellin R.
D. Di Majo, M. Giammanco, E. Tripoli, P. Gagliano, G. Tabacchi, S. Giammanco, M. La Guardia
Sect. Internal Medicine, Gerontol. Geriatry, Dept. Clin. and Exper. Med., University of Ferrara We evaluated all subjects addressing our Metabolic Center in a two-year period, looking for metabolic syndrome prevalence and cardiovascular disease association. Different definitions of the metabolic syndrome were considered: all appeared highly correlated. At last we categorized patients according to overweight (BMI>25 Kg/m2 in women and >30 in men), abnormal glucose level ~110 mg/dl)or diabetes, high blood pressure (~130/=85 mmHg) and a unified-dyslipidemic parameter (triglycerides a150 mg/dl and/or HDL-C <40 mg/dl in men and/or <50 mg/dl in women). This definition may be considered a variant of ATP-III proposal and 22.6% of all examined patients (n=1124, most of whom hypercholesterolemic) were recognized as carrier of at least 3 metabolic cdteria, while 4.5% had a full-expressed syndrome (4 criteria). The prevalence of subjects (particularly men) with metabolic syndrome (3 or 4 criteria) and documented coronary heart disease (CHD) was more than twice that of remaining patients (12.6% vs. 5.9%, p<0.001). Possible CHD (poor or incomplete clinical evidence) was similarly more prevalent among 'metabolic' cases than others (3.9% vs. 1.6%, p<0.01). Ill-defined CHD was more frequently found in women than in men (4.8% vs. 2.3%). These data have been confirmed also after stratification by age ~65 and >65 years). Peripheral arterial disease was 1.5-2 times more prevalent in patients with the metabolic syndrome than in other subjects, and this result was more significant in younger people ~65 years). We conclude that the metabolic cluster is associated with increased arterial disease also in this setting of high-risk dyslipidemic patients.
Monocyte/macrophages are known to play a relevant role in the development of atherosclerosis. Peroxisome proliferator-activated receptor-y(PPAR-~;) is an important regulator of genes controlling lipid and glucose metabolism, which is activated by naturally occurring ligands, including 15-deoxy-PGJ2 (15d-PGJ2) and ox-LDL, as well as by synthetic agents, such as the thiazolidinedione antidiabetics. Experimental evidences suggest that PPAR-y; can exert a relevant role in atherosclerosis. We investigated the expression of PPAR-y and CD-36 receptors in monocyte/macrophages of healthy smokers and nonsmokers. Monocytes (M) were collected from heparinized venous blood of healthy volunteers by standard techniques and purified by adhesion; monocyte-derived macrophages (MDM) were prepared from M cultured for 8 days in RPMI 1640 medium enriched with 20% FCS. PPAR-y and CD-36 expressions were evaluated by Immunoblotting and Flow cytometry, respectively. The basal expression of PPAR-y; is minimal in M (ratio PPAR-?;/6actin = 0.23 + 0.04; n=9), higher after 4 days of differentiation (0.33 + 0.03; n=9) and very relevant in MDM (0.55 + 0.04; n=9; p < 0.01). The expression of PPAR-y; in M and MDM is increased when the cells are incubated with 15d-PGJ2 (5 I~M, 6h) or ciglitazone (50 ~_M, 6h). Preliminary data indicate that the basal expression of PPAR-y; in healthy smokers is 2.5-3 fold higher than in non-smokers. Expression of CD-36 on M and MDM is about 50-60% in non-smokers and 70-80% in smokers. These results indicate that PPAR-y; is expressed in M and MDM and suggest a possible role in atherosclerosis.
Istituto di Fisiologia e Nutrizione Umana, Universit~ di Palermo, via Augusto Ella,3 -90127- Palermo Introduction: Coronary artery disease (CAD) is the most dangerous disease of the second half of the XX century. The scientific community has proposed some measures for reducing the high level of mortality from CAD.The pathogenetic basis of CAD is first of all the high level of cholesterol in blood.Recent studies suggest that one of the most important mechanisms predisposing to development of atherosclerosis is the oxidation of LDL-C particles. It has been demonstrated both in vitro and in vivo that the phenolic compounds can offer significant antiatherogenic protection by inhibiting the oxidation of LDL. Aim: Purpose of this work was to evaluate the antioxidant effectiveness and the phenolic composition of Sicilian red wines in comparison with French red wines. Methods: we analysed 24 red wines (11 Sicilian and 13 French). Total phenols were analysed according to the Folin-Ciocalteau method. Antioxidant capacity was determined by a method based on crocin bleaching by oxidation due to a source of radicals. Statistical data analysis was performed by the ANOVA test. Results: The concentration of total phenol varied from 3578 mg/L GAE to 2370 mg/L GAE for Sicilian red wines while in the French red wines is inclusive from 3100 mg/L GAE to 1533 mg/LGAE. The Sicilian red wines studied have shown higher levels of antioxidant capacity than the French wines. Conclusion: This study show that the amounts of phenols in Sicilian red wines is most abundant when compared to the other French samples. Moreover, this study evidences that the antioxidant activity is correlated with total phenol content of wine.(POSTER)