- Email: [email protected]
Lissencephaly with Hypoplasia of the Corpus Callosum and Cerebellum in a Domestic Cat
141:4, 2009
ESVP/ECVP Proceedings 2009
289
45
46
YERSINIA ENTEROCOLITICA INFECTION IN A VERVET MONKEY (CERCOPITHICUS AETHIOPS) IN IRAN M.H. Hablolvarid, Z. Sarebanan, R. Arefpajoohi, Motamedi and R. Gholam Razi Vaccine and Serum Research Institute, Karaj, Iran Introduction: Yersinia enterocolitica is one of the major species of the genus Yersinia and is widely distributed in nature in aquatic and animal reservoirs. It is primarily an enteric pathogen but, under defined host conditions, has a strong tendency for extra-intestinal spread. Swine serve as a major reservoir for human pathogenic strains. Materials and Methods: A male vervet monkey (Cercopithecus aethiops), in good bodily condition and housed in quarantine, died after a short illness. The most outstanding clinical signs were depression, anorexia, diarrhoea and dehydration. Multiple liver abscesses and enlargement of the spleen with pinpoint white spots were the most prominent gross features. Samples of different organs were collected and referred for further examination to the Departments of Bacteriology, Parasitology and Pathology. Results: Bacteriological examination of different organs yielded Y. enterocolitica. Lesions of acute hepatitis, splenitis and enteritis were evident on histological examination. Conclusions: This is the first report of fatal yersiniosis in a vervet monkey in Iran. Transmissible pathogenic and opportunistic zoonotic enteric bacteria, such as Y. enterocolitica, comprise a recognized occupational health hazard and those working with non-human primates in exotic animal quarantine stations must take adequate precautions.
CUTANEOUS MAST CELL TUMOUR IN A HOLSTEIN HEIFER H. Azarabad *, I. Nowrouzian y, E. Soliemany z, M. Aramoon x, R. Azarabad { and S. Azarabadk *Department of Veterinary Pathology, Tehran, yDepartment of Veterinary Clinical Science, University of Tehran, zArtesh University of Medical Science, Department of Veterinary Pathology, Tehran, xFaculty of Veterinary Medicine, University of Tehran, {IRAN University of Medical Science, Tehran and k Azad University of Medical Science, Tehran, Iran Introduction: Mast cell tumours are uncommon in cattle and account for only 3% of cutaneous and subcutaneous bovine tumours. Scant data suggest that cutaneous tumours in cattle are associated with visceral mast cell aggregates, although purely cutaneous tumors have been reported. Materials and Methods: An 8-month-old Holstein heifer presented with nodular pruritic skin lesions on the face and neck. The nodules measured 2 e 4 cm in diameter, were non-encapsulated, had a rubbery texture and were grey to light yellow colouration. Excision biopsies of skin nodules were fixed in 10% neutral buffered formalin and stained with haematoxylin and eosin and Giemsa. The animal was necropsied and viscera were examined microscopically. Results: Histological examination of the cutaneous nodules showed sheets of round cells and occasional eosinophils in the dermis and subcutis. The round cells had uniform, centrally located round to oval nuclei surrounded by clear to amphophilic cytoplasm and well-defined cell borders. Cytoplasmic granules in Giemsa-stained sections were few. The appearance of the tumour was of a grade II mastocytoma. No visceral mast cell aggregates were detected. Conclusions: A cutaneous mast cell tumor was diagnosed in a Holstein heifer.
47 THE EFFECT OF PROBIOTIC (BACILLUS SPP) ON GROWTH, SURVIVAL, AND INNATE IMMUNITY OF RAINBOW TROUT (ONCHORHYNCHUS MYKISS) FRY DURING THE FIRST TWO MONTHS OF FEEDING A. Hedayati *,y and T. Bagheri* *Department of Marine Biology, Khorramshahr University of Marine Science & Technology, Khorramshahr and yDepartment of Fishery, Gorgan University of Agricultural Science, Gorgan, Iran Introduction: A commercial Bacillus spp. probiotic was tested on rainbow trout fry during the first two months of feeding. Materials and Methods: Probiotic was introduced into diets at five different levels, (T1:4.8 108, T2:1.2 109, T3:2.01 109, T4:3.8 109, T5:6.1 109 CFU g-1). The effects were compared with those of a control diet containing no probiotic. Results: Survival in treatment groups was significantly (P ! 0.05) higher than in controls despite slightly increased mortality during the first week. Bacterial counts in the intestine in all treatment groups were significantly (P ! 0.05) higher than in controls and Bacillus spp. was not detected in controls. Specific growth rate, condition factor and protein efficiency ratio were significantly (P ! 0.05) higher and feed conversion ratio was lower in groups receiving probiotic than in controls. Growth performance in the treatment group receiving 3.8 109 CFU g-1 showed the best results. Conclusions: Supplementation of diets with probiotic appears to stimulate digestive development and enzymatic activity in fish. Dosage of probiotic should be assessed before application on a large scale to avoid any undesired effects. The supplementation of trout starter diets with Bacillus spp. is probably beneficial for rearing conditions.
48 LISSENCEPHALY WITH HYPOPLASIA OF THE CORPUS CALLOSUM AND CEREBELLUM IN A DOMESTIC CAT A. Herrmann, W. Hecht and C. Herden Justus-Liebig-University, Giessen, Germany Introduction: An approximately 2-year-old domestic cat was submitted for necropsy examination with a history of recurrent aggressive behaviour and sensory disturbances. Materials and Methods: Following necropsy and histopathological evaluation, neurofilament (NF) and glial fibrillary acidic protein (GFAP) were demonstrated immunohistochemically. Polymerase chain reaction and subsequent sequencing of exons 2, 3 and 4 of the TUBA1A gene were completed. Results: Gross pathological evaluation revealed a hypoplastic cranial cavity containing a small brain with only marginal formation of gyri and sulci in both cerebral hemispheres. The cerebellum was hypoplastic and the corpus callosum lacked midline unification. Histologically, areas of white matter were markedly reduced. Abundant swollen disorderly axons labelled positively for NF. Using GFAP immunolabelling, nests of plump, activated astrocytes were demonstrated in the cortical white matter. Sequencing of exons 2 to 4 of the TUBA1A gene revealed no potential disease causing mutations. Conclusions: This is the first report of lissencephaly in a domestic cat. Malformations of the corpus callosum combined with cerebellar hypoplasia are common in man but have not been described in the veterinary literature. Many of these malformations in man are related to mutations in exon 4 of the TUBA1A gene. Corresponding mutations were not detected in the present case. Other regions of the gene or other candidate genes such as the LISS gene may be involved.
ESVP/ECVP Proceedings 2009
289
45
46
YERSINIA ENTEROCOLITICA INFECTION IN A VERVET MONKEY (CERCOPITHICUS AETHIOPS) IN IRAN M.H. Hablolvarid, Z. Sarebanan, R. Arefpajoohi, Motamedi and R. Gholam Razi Vaccine and Serum Research Institute, Karaj, Iran Introduction: Yersinia enterocolitica is one of the major species of the genus Yersinia and is widely distributed in nature in aquatic and animal reservoirs. It is primarily an enteric pathogen but, under defined host conditions, has a strong tendency for extra-intestinal spread. Swine serve as a major reservoir for human pathogenic strains. Materials and Methods: A male vervet monkey (Cercopithecus aethiops), in good bodily condition and housed in quarantine, died after a short illness. The most outstanding clinical signs were depression, anorexia, diarrhoea and dehydration. Multiple liver abscesses and enlargement of the spleen with pinpoint white spots were the most prominent gross features. Samples of different organs were collected and referred for further examination to the Departments of Bacteriology, Parasitology and Pathology. Results: Bacteriological examination of different organs yielded Y. enterocolitica. Lesions of acute hepatitis, splenitis and enteritis were evident on histological examination. Conclusions: This is the first report of fatal yersiniosis in a vervet monkey in Iran. Transmissible pathogenic and opportunistic zoonotic enteric bacteria, such as Y. enterocolitica, comprise a recognized occupational health hazard and those working with non-human primates in exotic animal quarantine stations must take adequate precautions.
CUTANEOUS MAST CELL TUMOUR IN A HOLSTEIN HEIFER H. Azarabad *, I. Nowrouzian y, E. Soliemany z, M. Aramoon x, R. Azarabad { and S. Azarabadk *Department of Veterinary Pathology, Tehran, yDepartment of Veterinary Clinical Science, University of Tehran, zArtesh University of Medical Science, Department of Veterinary Pathology, Tehran, xFaculty of Veterinary Medicine, University of Tehran, {IRAN University of Medical Science, Tehran and k Azad University of Medical Science, Tehran, Iran Introduction: Mast cell tumours are uncommon in cattle and account for only 3% of cutaneous and subcutaneous bovine tumours. Scant data suggest that cutaneous tumours in cattle are associated with visceral mast cell aggregates, although purely cutaneous tumors have been reported. Materials and Methods: An 8-month-old Holstein heifer presented with nodular pruritic skin lesions on the face and neck. The nodules measured 2 e 4 cm in diameter, were non-encapsulated, had a rubbery texture and were grey to light yellow colouration. Excision biopsies of skin nodules were fixed in 10% neutral buffered formalin and stained with haematoxylin and eosin and Giemsa. The animal was necropsied and viscera were examined microscopically. Results: Histological examination of the cutaneous nodules showed sheets of round cells and occasional eosinophils in the dermis and subcutis. The round cells had uniform, centrally located round to oval nuclei surrounded by clear to amphophilic cytoplasm and well-defined cell borders. Cytoplasmic granules in Giemsa-stained sections were few. The appearance of the tumour was of a grade II mastocytoma. No visceral mast cell aggregates were detected. Conclusions: A cutaneous mast cell tumor was diagnosed in a Holstein heifer.
47 THE EFFECT OF PROBIOTIC (BACILLUS SPP) ON GROWTH, SURVIVAL, AND INNATE IMMUNITY OF RAINBOW TROUT (ONCHORHYNCHUS MYKISS) FRY DURING THE FIRST TWO MONTHS OF FEEDING A. Hedayati *,y and T. Bagheri* *Department of Marine Biology, Khorramshahr University of Marine Science & Technology, Khorramshahr and yDepartment of Fishery, Gorgan University of Agricultural Science, Gorgan, Iran Introduction: A commercial Bacillus spp. probiotic was tested on rainbow trout fry during the first two months of feeding. Materials and Methods: Probiotic was introduced into diets at five different levels, (T1:4.8 108, T2:1.2 109, T3:2.01 109, T4:3.8 109, T5:6.1 109 CFU g-1). The effects were compared with those of a control diet containing no probiotic. Results: Survival in treatment groups was significantly (P ! 0.05) higher than in controls despite slightly increased mortality during the first week. Bacterial counts in the intestine in all treatment groups were significantly (P ! 0.05) higher than in controls and Bacillus spp. was not detected in controls. Specific growth rate, condition factor and protein efficiency ratio were significantly (P ! 0.05) higher and feed conversion ratio was lower in groups receiving probiotic than in controls. Growth performance in the treatment group receiving 3.8 109 CFU g-1 showed the best results. Conclusions: Supplementation of diets with probiotic appears to stimulate digestive development and enzymatic activity in fish. Dosage of probiotic should be assessed before application on a large scale to avoid any undesired effects. The supplementation of trout starter diets with Bacillus spp. is probably beneficial for rearing conditions.
48 LISSENCEPHALY WITH HYPOPLASIA OF THE CORPUS CALLOSUM AND CEREBELLUM IN A DOMESTIC CAT A. Herrmann, W. Hecht and C. Herden Justus-Liebig-University, Giessen, Germany Introduction: An approximately 2-year-old domestic cat was submitted for necropsy examination with a history of recurrent aggressive behaviour and sensory disturbances. Materials and Methods: Following necropsy and histopathological evaluation, neurofilament (NF) and glial fibrillary acidic protein (GFAP) were demonstrated immunohistochemically. Polymerase chain reaction and subsequent sequencing of exons 2, 3 and 4 of the TUBA1A gene were completed. Results: Gross pathological evaluation revealed a hypoplastic cranial cavity containing a small brain with only marginal formation of gyri and sulci in both cerebral hemispheres. The cerebellum was hypoplastic and the corpus callosum lacked midline unification. Histologically, areas of white matter were markedly reduced. Abundant swollen disorderly axons labelled positively for NF. Using GFAP immunolabelling, nests of plump, activated astrocytes were demonstrated in the cortical white matter. Sequencing of exons 2 to 4 of the TUBA1A gene revealed no potential disease causing mutations. Conclusions: This is the first report of lissencephaly in a domestic cat. Malformations of the corpus callosum combined with cerebellar hypoplasia are common in man but have not been described in the veterinary literature. Many of these malformations in man are related to mutations in exon 4 of the TUBA1A gene. Corresponding mutations were not detected in the present case. Other regions of the gene or other candidate genes such as the LISS gene may be involved.