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Localization of tissue transglutaminase in the developing spinal cord
A2
9th
Meetingof the ESN
5 EFFECT OF VISUAL DEPRIVATION ON DEVELOPMENT OF CBOLINERGIC, GABAERGIC AND GLUTAMATERGIC MARKERS IN RAT VISUAL CORTEX.
6
LOCALIZATION OF TISSUE TRAESGLUTMILIASE IN THE DEVEMPING SPINAL CORD. & L.W.Haynes. Zoology, University of Woodland Rd. ES8 1UG. U.K.
M.J.M.Perry
Department Bristol,
Reinhard Schliebs and Ashok Kumar Paul Flechsig Institute for Brain Research, Leipzig, Germany The postnatal development laminar pattern of
Tissue transglutaminase (tTG) is a activated retinoid-inducible calcium enzyme which forms covalent isopaptide cross links between substrate proteins to form insoluble complexes. tTG is active in forming believed to be apoptotic bodies during spontaneous (L retinoid induced programmed cell death. tTG activity was measured in rat spinal cord during the perinatal period. Maxima of activity occurred during the late parinatal & early postnatal periods. During this time there was a shift from the cytosolic to the particulate form of
of the
repine receptors as well as NMDA and using AMPA receptors were studied receptor autoradiography. The autoradiograms obtained were quantitatively evaluated by computer-assisted image The laminar pattern of analysis. receptor binding sites studied changed during postnatal ontogenesis. At postn;tal day 1 the densitiTsa;i Ml-and M -mAChR as well as Ca inase C binding sites were protein still homogeneously distributed throughout the cortex, whereas during the maturation of the visual cortex a distinct laminar pattern is formed which is differently affected by monocular visual deprivation.
the enzyme. Activity maxima coincided with the appearance of immunoreactive endothelia a tTG in vascular It is proposed that tTG motoneurones. motoneurones undergoing appears in phase2 (histiotypic) cell death.
8
7
EMBRYONIC TRANSCRIPT.
of
EXPRESSION
OF
DM-20
S.G. Timait', D.R. Colman* and 8. Zalc'. 75651, Paris OINSERM U-134, Hop. Salpetriere, Univ., Dept of Cedex 13, France and *Columbia Anat. and Cell Biol., New York, NY, 10032, USA.
I&CAL
SYNTliNSIS
OF AXOPLASbiIC
PROTEINS:
TN&
ORETICAC CONSIDERATIONS. Jaime Alvarez. Unidad de Neurobiologla cular. Universidad Cat6lica. Santiago,
MoleChile
It is believed that the perikaryon is the sole source of neuronal proteins, and proteins move distalward at we used both the polymerase chain redction (P(.Hl that axoskeletal hybridization to search for the presence and in situ a slow rate. These notions lead to inconsisof proteolipid protein (PLP) gene transcripts in the tencies and ad hoc assumptions need be added developing mouse. Total brain RNA extracted from El3 time and again to remove contradictions. demonstrated the to E19 embryos, analyzed by PCR, axoskeletal proE.g., to reach the endings, which was sin-jle transcript presence Of a teins are assumed to be stable, unambiguously identified with the DM-20 mRNA. RNA even for two signals P2 arimals showed samples from years, which is against experimental data. corresponding to W-20 and PLP transcripts. By in situ On the other hand, a local source of using a DM-20 antisense hybridization, of El0 embryos, proteins can account for the steady state of we showed that the localization of the DMcRNA probe. the axoplasm as well as the slow radioactive a0 message was restricted to the diencephalic basal waves, phenomena considered so far to supplate. On the same embryo sections, in addition to the brain localization, an intense hybridizing signal was port the slow transport. Many observations niso detected in the trigeminal and spinal ganylia, not easy to frame in the previous theory ganglion, and ihe vaga: glossopharyngeal tile find in the local source a straightforward sympathetic ganglion chain. Topographical analysis 3f explanation, inhibitors of protein e.g., 3M-20 expressing cells was performed by in situ reduce synthesis substantially the hybr.dizarion ;f Eli, El>, axonal EIR dno P2 ani.mals. The dcmanirrnt_cn o! transcripti:,,: of rhe PI,P qene, :"ng cytoskeleton. Moreover, the local source rhi, rnye1 i!,,i: ,<>I, procrs5, betore the beqinrir>q :>t makes unnecessary the elusive mechanism of func!_ior: in suggcsrs thai ,:I addit :cr to d s:i~ct,,rdL translocation of axoskeletal proteins since !vyel:-1 compacriol, 'ilrli,cf _,I<' prc**,,:!.5 0' lh<, ?‘.? in this view these are immobile. ycrf? (DM-201 Iray :1ave ? L3_C. /r Lhr rliffe,r": .dil:" 3f In brief, our understanding of the maint'ie "F.lra t !IDD. tenance of the neuron needs a major revision Supported by grant FONDECYT 660-92.
9th
Meetingof the ESN
5 EFFECT OF VISUAL DEPRIVATION ON DEVELOPMENT OF CBOLINERGIC, GABAERGIC AND GLUTAMATERGIC MARKERS IN RAT VISUAL CORTEX.
6
LOCALIZATION OF TISSUE TRAESGLUTMILIASE IN THE DEVEMPING SPINAL CORD. & L.W.Haynes. Zoology, University of Woodland Rd. ES8 1UG. U.K.
M.J.M.Perry
Department Bristol,
Reinhard Schliebs and Ashok Kumar Paul Flechsig Institute for Brain Research, Leipzig, Germany The postnatal development laminar pattern of
Tissue transglutaminase (tTG) is a activated retinoid-inducible calcium enzyme which forms covalent isopaptide cross links between substrate proteins to form insoluble complexes. tTG is active in forming believed to be apoptotic bodies during spontaneous (L retinoid induced programmed cell death. tTG activity was measured in rat spinal cord during the perinatal period. Maxima of activity occurred during the late parinatal & early postnatal periods. During this time there was a shift from the cytosolic to the particulate form of
of the
repine receptors as well as NMDA and using AMPA receptors were studied receptor autoradiography. The autoradiograms obtained were quantitatively evaluated by computer-assisted image The laminar pattern of analysis. receptor binding sites studied changed during postnatal ontogenesis. At postn;tal day 1 the densitiTsa;i Ml-and M -mAChR as well as Ca inase C binding sites were protein still homogeneously distributed throughout the cortex, whereas during the maturation of the visual cortex a distinct laminar pattern is formed which is differently affected by monocular visual deprivation.
the enzyme. Activity maxima coincided with the appearance of immunoreactive endothelia a tTG in vascular It is proposed that tTG motoneurones. motoneurones undergoing appears in phase2 (histiotypic) cell death.
8
7
EMBRYONIC TRANSCRIPT.
of
EXPRESSION
OF
DM-20
S.G. Timait', D.R. Colman* and 8. Zalc'. 75651, Paris OINSERM U-134, Hop. Salpetriere, Univ., Dept of Cedex 13, France and *Columbia Anat. and Cell Biol., New York, NY, 10032, USA.
I&CAL
SYNTliNSIS
OF AXOPLASbiIC
PROTEINS:
TN&
ORETICAC CONSIDERATIONS. Jaime Alvarez. Unidad de Neurobiologla cular. Universidad Cat6lica. Santiago,
MoleChile
It is believed that the perikaryon is the sole source of neuronal proteins, and proteins move distalward at we used both the polymerase chain redction (P(.Hl that axoskeletal hybridization to search for the presence and in situ a slow rate. These notions lead to inconsisof proteolipid protein (PLP) gene transcripts in the tencies and ad hoc assumptions need be added developing mouse. Total brain RNA extracted from El3 time and again to remove contradictions. demonstrated the to E19 embryos, analyzed by PCR, axoskeletal proE.g., to reach the endings, which was sin-jle transcript presence Of a teins are assumed to be stable, unambiguously identified with the DM-20 mRNA. RNA even for two signals P2 arimals showed samples from years, which is against experimental data. corresponding to W-20 and PLP transcripts. By in situ On the other hand, a local source of using a DM-20 antisense hybridization, of El0 embryos, proteins can account for the steady state of we showed that the localization of the DMcRNA probe. the axoplasm as well as the slow radioactive a0 message was restricted to the diencephalic basal waves, phenomena considered so far to supplate. On the same embryo sections, in addition to the brain localization, an intense hybridizing signal was port the slow transport. Many observations niso detected in the trigeminal and spinal ganylia, not easy to frame in the previous theory ganglion, and ihe vaga: glossopharyngeal tile find in the local source a straightforward sympathetic ganglion chain. Topographical analysis 3f explanation, inhibitors of protein e.g., 3M-20 expressing cells was performed by in situ reduce synthesis substantially the hybr.dizarion ;f Eli, El>, axonal EIR dno P2 ani.mals. The dcmanirrnt_cn o! transcripti:,,: of rhe PI,P qene, :"ng cytoskeleton. Moreover, the local source rhi, rnye1 i!,,i: ,<>I, procrs5, betore the beqinrir>q :>t makes unnecessary the elusive mechanism of func!_ior: in suggcsrs thai ,:I addit :cr to d s:i~ct,,rdL translocation of axoskeletal proteins since !vyel:-1 compacriol, 'ilrli,cf _,I<' prc**,,:!.5 0' lh<, ?‘.? in this view these are immobile. ycrf? (DM-201 Iray :1ave ? L3_C. /r Lhr rliffe,r": .dil:" 3f In brief, our understanding of the maint'ie "F.lra t !IDD. tenance of the neuron needs a major revision Supported by grant FONDECYT 660-92.