- Email: [email protected]
Localized argyria resulting from implanted acupuncture needles — A case report with some new observations
410
POSSIBLE SYSTEMICEFFECT OF SUNSCREENTOPICALAPPLICATION OBSFXVED EY 'IREEAX SWELLINGOF MOUSE Y.P. KIil,and S.C. LEE Departmwt of Dermatoiogy,ChonnamUniv. Med. School, Kwqju-City, Korea Adult Swiss nice of both sex were irradiatedwith W-B on the back; ear swLling was neasurerl ni.tba dial thickness gauge at 0, 3, 24, and 48h after irradiation(i.OSJ/cn'); Sunscreen cream(SSC)was applied30 min before UV irradiation: two groups were applied on the shaven back skin with either SPF 2 or 3O(Sundown,Johnsotiohnson), and one as control(C). The active ingradientsof sunscreenswere octyl methoxycinnaoats. octvl salicvlate.o::vixnzone. and titaniumdioxide.At B, farm&ickness'incr&se~ by 27~&m(M'&D) in C, while the SPF 8 increasedby 17t.10and the SPF 30 by 725. At 24b, both SPF groups significantlyinhibitedear swellingcomparedwith C. Zven riienears were shieled,ear swellingoccured, though :.ess,upon UV-B irradiationon the back(3h-13f15;24h-40*24; 43h=147?93),and these effectswere significantlyinhibited by SSC(SPF i3=72"38; SPF 30=50?37at 43h). SSC were applied on the abdominalskin and W-B irradiatedon the back. The inhibitory effectsby SSC were also evident after 24h, but not after 48h.
E6 GENES, BUT NOT E7 GENES, OF HUMAN PAPILLOMAVIRUS 16 AND 47 BELONGING TO DIFFERENT VIRAL SUBGROUPS ARE SIMILAR FOR THEIR TRANSFORMING ACTIVITIES ON A FIBROBLASTIC CFLL LINE OF RAT. ATURO HIRAIWA, AYUMI ADACHI AND MASARU OHASHI Department of Dermatology,.Schoolof Medicine. Nagoya University, Nagoya We previously reported that E6 gene of human papillomavirus (HPV) 47 (associatedwith epidermodysplasiaverrwiformis) can induce a week, but definite, morphological transformation of a rat fibroblastic cell line, 3Y1, whereas its E7 gene can not. The present study has bee" carried out to examine whether E6 genes of other HPVs rather distantly related to HW-47 bear similar activity, and has come to a conclusion that E6 genes of HPV-16 (associatedwith cervical cancer) bear the activity no weaker than that of HPV-47, whereas those of HFV-la (associatedwith plantar wart) and -11 (associatedwith condyloma) bear weaker activity. We will present data supporting the above conclusion and discuss its implication. This work has been done in colaboratio"with Tohru Kiyono and Masahide Ishibashi of Laboratory of Viral Oncology, Aichi Cancer Center.
INCREASED PLASMA HISTAMINE LEVELS IN ATOPIC DERMATITIS NAOKO ISHIGURO, YIJKOHIGAKI, AND MAKOTO KAWASHIMA Department of Dermatology, Tokyo Women's Medical College, Tokyo
Kycung Cha~~Park,YcungMi.nChoi*,Ym ShinLee Eqa%"entofDermatolcgya"dqneccllqy*, Collegeof Medicine, SeoulNaticnaltiversity, Seoul,Koree
Plasma histamine levels were assessed in fifty five patients with atopic dermatitis (AD) and in twenty three healthy controls by radio-immunoassay. AD patients showed significantly elevated plasma histamine level (2.12nM) as compared to healthy controls (1.44"M) (pt0.005). Patients with severe dermatitis showed relatively high plasma histamine level. There were no significant correlations between plasma histamine level and clinical parameters such as eerum IgE, LDH, and blood eosinophil count. The coexistance of background of respiratory atopy did not seem to influence plasma histamine: -As plasma histamine level could reflect the released histamine level in the skin. these findings suaest that histamine may play a role i; the exacerbation 07 AD.
Ccmdyla"3aw&la tiis accntagicus disease causedby the Hun3n papillaraviruses&IPV). The-entepidemic of genital wart.sis~tIMnifestinywng adults. Hwever several reports points to increased cases i" infants andycung children. Y-g adults acquiregenitalwarts by sexual ccntact, but the modeof transnissicn in infants andchilclrenisless clear. Until1~~ve9.y snail n-of genitalwarts cawed inchildrenwere exHRined for the presenceof HPVDNA.
Transfection of H"OLe" Keratinocytes Papillose"irusType-l& -2a and -4 DNA
with
Human
AKIH1RO s*x*o, M1CHIO NAKANO, MASAAKI "OROHAs"I, MASAHIKO KUROKAwA*, "IROSHI OCHIAI*. Medical L Departud?"t Of rJermato1cqy. *Virology, ToyPharmaceutical
University,
all sar&s and same as respsible for axgenital warts of adults. 2llerewere fanilyhistoq of genital-s in saw cases. These findingsauggestgenitalwarts inchildrenmay
betransnittedbypersonalccntactf~parentsratherthan sexual abuse.
LOCALIZED ARGYRIA RESULTING FROM IMPLANTED ACUPUNCTURE NEEDLES ------ A CASE REPORT WITH SOME NEW OBSBRVATIONS. KAN NIIZUMA, OSAMU YAMAMOTO, MUNEO OHKIDO, AND *YASUO MIYAMOTO. Department of Dermatology, Tokai University School of Medicine, *Department of Metallurgical Engineering,School of Engineering, Tokai University., Kanagawa, Japan.
Toyama.
There are over 60 typee of human papillomevirus (HW) know" -2 end -4 are the mo*t _" type* to to date. "W-l, induce benign papillomatous lesion. The lack of in vitro limits the propagation of HW SYstem allowina the biological analysis of HW in cutaneous papillomas. I" order to develop in vitro experiment system, we attempted to trensfect normal hunan keretinccytes with HW genanes keratinocytes were liolTd1 human -2a or -4). HW gemme obtained from the cultured at 37'c in K-GM. favoring conditions PHW was ligated under plsmid HP" tIPI* wee The ligated 1iqation. i"tC3llloleCUler
t-la.
tsansfeoted
Recentintrc&ctionof PCR enable sensitivedetectionof theseviral mbetter thanbefore. HPVLNAwas detectedin
into -cultured
keeratin‘xytes by
Polybrane
DNA was detected in kerati"ocytee eve" 1 technique. HW week after transfectio" by blot hybridization. Now. the DNA and morphological transfected e*preesion level Of chanpe of transfected cells are studied.
A case of the so-called disseminated localized argyria resulting from implanted acupuncture needles was studied by optical and electron microscopy. A 68-year-old Japanese female had several small pigmented macular lesions which aggregated at the middle of forehead. at the rlaht tip of elbow, and at the external malleolus of.right foot:The lesions had gradually increased in number and size for over 20 years. When she was 48-yeas-old about 50 pieces of acupuncture needles were implanted in her shoulders and limbs for stiff shoulders and eonaleia. The foci in H&E sections consisted mostly of deposition of fine black granules were spread around the eccrine sweat g1ands.i" the blood vessel walls,and along the elastic fibers. Electron probe microanalyser(EPMA)study of the fine black granules "sing ultra thin sections revealed that most granules consisted of silver and se1e"ium.A" EPMA study also revealed that the needles were made of a special sort of brass and plated with silver.
POSSIBLE SYSTEMICEFFECT OF SUNSCREENTOPICALAPPLICATION OBSFXVED EY 'IREEAX SWELLINGOF MOUSE Y.P. KIil,and S.C. LEE Departmwt of Dermatoiogy,ChonnamUniv. Med. School, Kwqju-City, Korea Adult Swiss nice of both sex were irradiatedwith W-B on the back; ear swLling was neasurerl ni.tba dial thickness gauge at 0, 3, 24, and 48h after irradiation(i.OSJ/cn'); Sunscreen cream(SSC)was applied30 min before UV irradiation: two groups were applied on the shaven back skin with either SPF 2 or 3O(Sundown,Johnsotiohnson), and one as control(C). The active ingradientsof sunscreenswere octyl methoxycinnaoats. octvl salicvlate.o::vixnzone. and titaniumdioxide.At B, farm&ickness'incr&se~ by 27~&m(M'&D) in C, while the SPF 8 increasedby 17t.10and the SPF 30 by 725. At 24b, both SPF groups significantlyinhibitedear swellingcomparedwith C. Zven riienears were shieled,ear swellingoccured, though :.ess,upon UV-B irradiationon the back(3h-13f15;24h-40*24; 43h=147?93),and these effectswere significantlyinhibited by SSC(SPF i3=72"38; SPF 30=50?37at 43h). SSC were applied on the abdominalskin and W-B irradiatedon the back. The inhibitory effectsby SSC were also evident after 24h, but not after 48h.
E6 GENES, BUT NOT E7 GENES, OF HUMAN PAPILLOMAVIRUS 16 AND 47 BELONGING TO DIFFERENT VIRAL SUBGROUPS ARE SIMILAR FOR THEIR TRANSFORMING ACTIVITIES ON A FIBROBLASTIC CFLL LINE OF RAT. ATURO HIRAIWA, AYUMI ADACHI AND MASARU OHASHI Department of Dermatology,.Schoolof Medicine. Nagoya University, Nagoya We previously reported that E6 gene of human papillomavirus (HPV) 47 (associatedwith epidermodysplasiaverrwiformis) can induce a week, but definite, morphological transformation of a rat fibroblastic cell line, 3Y1, whereas its E7 gene can not. The present study has bee" carried out to examine whether E6 genes of other HPVs rather distantly related to HW-47 bear similar activity, and has come to a conclusion that E6 genes of HPV-16 (associatedwith cervical cancer) bear the activity no weaker than that of HPV-47, whereas those of HFV-la (associatedwith plantar wart) and -11 (associatedwith condyloma) bear weaker activity. We will present data supporting the above conclusion and discuss its implication. This work has been done in colaboratio"with Tohru Kiyono and Masahide Ishibashi of Laboratory of Viral Oncology, Aichi Cancer Center.
INCREASED PLASMA HISTAMINE LEVELS IN ATOPIC DERMATITIS NAOKO ISHIGURO, YIJKOHIGAKI, AND MAKOTO KAWASHIMA Department of Dermatology, Tokyo Women's Medical College, Tokyo
Kycung Cha~~Park,YcungMi.nChoi*,Ym ShinLee Eqa%"entofDermatolcgya"dqneccllqy*, Collegeof Medicine, SeoulNaticnaltiversity, Seoul,Koree
Plasma histamine levels were assessed in fifty five patients with atopic dermatitis (AD) and in twenty three healthy controls by radio-immunoassay. AD patients showed significantly elevated plasma histamine level (2.12nM) as compared to healthy controls (1.44"M) (pt0.005). Patients with severe dermatitis showed relatively high plasma histamine level. There were no significant correlations between plasma histamine level and clinical parameters such as eerum IgE, LDH, and blood eosinophil count. The coexistance of background of respiratory atopy did not seem to influence plasma histamine: -As plasma histamine level could reflect the released histamine level in the skin. these findings suaest that histamine may play a role i; the exacerbation 07 AD.
Ccmdyla"3aw&la tiis accntagicus disease causedby the Hun3n papillaraviruses&IPV). The-entepidemic of genital wart.sis~tIMnifestinywng adults. Hwever several reports points to increased cases i" infants andycung children. Y-g adults acquiregenitalwarts by sexual ccntact, but the modeof transnissicn in infants andchilclrenisless clear. Until1~~ve9.y snail n-of genitalwarts cawed inchildrenwere exHRined for the presenceof HPVDNA.
Transfection of H"OLe" Keratinocytes Papillose"irusType-l& -2a and -4 DNA
with
Human
AKIH1RO s*x*o, M1CHIO NAKANO, MASAAKI "OROHAs"I, MASAHIKO KUROKAwA*, "IROSHI OCHIAI*. Medical L Departud?"t Of rJermato1cqy. *Virology, ToyPharmaceutical
University,
all sar&s and same as respsible for axgenital warts of adults. 2llerewere fanilyhistoq of genital-s in saw cases. These findingsauggestgenitalwarts inchildrenmay
betransnittedbypersonalccntactf~parentsratherthan sexual abuse.
LOCALIZED ARGYRIA RESULTING FROM IMPLANTED ACUPUNCTURE NEEDLES ------ A CASE REPORT WITH SOME NEW OBSBRVATIONS. KAN NIIZUMA, OSAMU YAMAMOTO, MUNEO OHKIDO, AND *YASUO MIYAMOTO. Department of Dermatology, Tokai University School of Medicine, *Department of Metallurgical Engineering,School of Engineering, Tokai University., Kanagawa, Japan.
Toyama.
There are over 60 typee of human papillomevirus (HW) know" -2 end -4 are the mo*t _" type* to to date. "W-l, induce benign papillomatous lesion. The lack of in vitro limits the propagation of HW SYstem allowina the biological analysis of HW in cutaneous papillomas. I" order to develop in vitro experiment system, we attempted to trensfect normal hunan keretinccytes with HW genanes keratinocytes were liolTd1 human -2a or -4). HW gemme obtained from the cultured at 37'c in K-GM. favoring conditions PHW was ligated under plsmid HP" tIPI* wee The ligated 1iqation. i"tC3llloleCUler
t-la.
tsansfeoted
Recentintrc&ctionof PCR enable sensitivedetectionof theseviral mbetter thanbefore. HPVLNAwas detectedin
into -cultured
keeratin‘xytes by
Polybrane
DNA was detected in kerati"ocytee eve" 1 technique. HW week after transfectio" by blot hybridization. Now. the DNA and morphological transfected e*preesion level Of chanpe of transfected cells are studied.
A case of the so-called disseminated localized argyria resulting from implanted acupuncture needles was studied by optical and electron microscopy. A 68-year-old Japanese female had several small pigmented macular lesions which aggregated at the middle of forehead. at the rlaht tip of elbow, and at the external malleolus of.right foot:The lesions had gradually increased in number and size for over 20 years. When she was 48-yeas-old about 50 pieces of acupuncture needles were implanted in her shoulders and limbs for stiff shoulders and eonaleia. The foci in H&E sections consisted mostly of deposition of fine black granules were spread around the eccrine sweat g1ands.i" the blood vessel walls,and along the elastic fibers. Electron probe microanalyser(EPMA)study of the fine black granules "sing ultra thin sections revealed that most granules consisted of silver and se1e"ium.A" EPMA study also revealed that the needles were made of a special sort of brass and plated with silver.