ORAL AND MAXILLOFACIAL PATHOLOGY e214 Abstracts CARLOS BARBOSA FERREIRA, ELISMAURO FRANCISCO DE MENDONÇA. UNIVERSIDADE FEDERAL DE GOIÁS. The treatment of cancer of the oral cavity produces various localized adverse effects that can lead to the interruption of radiotherapy and hence influence patient survival. To reduce such effects, preventive dental protocols are established before, during and after treatment. This study sought to determine the influence of signing up for preventive dental protocols on the survival of patients with oral cavity cancer. Materials and Methods: A total of 89 patients with nonmetastatic squamous cell carcinoma of the oral cavity between 1989 and 2009, all of whom had undergone radiation therapy, were categorized based on time spent signed up for the dental protocol. Results: Those patients signed up for more than 6 months presented higher survival rates when compared to other groups (p ¼ 0.016). Patients monitored by dental teams suffer less severe adverse effects of radiotherapy and thereby have a lower risk of interruption of treatment, leading to a longer survival. Conclusion: Patients who signed up for the preventive protocol for more than 6 months survived longer.
PE-420 - IS THE PODOPLANIN ASSOCIATED WITH EZRIN PART OF THE PROCESS OF INVASION BY AMELOBLASTOMAS? YARA FRANCINE COSTA, KELLEN CRISTINE TJIOE, SUELY NONOGAKI, FERNANDO AUGUSTO SOARES, JOSÉ ROBERTO PEREIRA LAURIS, DENISE TOSTES OLIVEIRA. FACULDADE DE ODONTOLOGIA DE BAURU - UNIVERSIDADE DE SÃO PAULO. The association between podoplanin and ezrin in the process of odontogenic tumor invasion has not been studied previously. This paper investigated the relationship between podoplanin and ezrin expression in the odontogenic epithelium of ameloblastomas. Forty-seven ameloblastomas were analyzed by immunohistochemistry using anti-podoplanin and anti-ezrin antibodies. The association between both proteins was evaluated by applying chi-square and Spearman correlation coefficient using a statistical significance level of 0.05. Statistically significant differences between podoplanin expression in peripheral and central cells of tumors was observed (p < 0.001). Cytoplasmic expression of ezrin in the peripheral cells of ameloblastomas was stronger than membranous expression (p < 0.001). A positive correlation between podoplanin and ezrin was found but it did not reach statistical significance (p 0.05). Although all ameloblastomas showed intense podoplanin and ezrin expression in odontogenic epithelium, these proteins do not seem to act together in the process of tumor invasiveness. FAPESP (2012/ 08278-5 e 2012/13411-6).
PE-421 - LASER THERAPY-ASSOCIATED TOPICAL GROWTH FACTORS FOR TREATMENT OF ORAL MUCOSITIS IN PATIENTS UNDERGOING BONE MARROW TRANSPLANTATION. CATARINA DA MOTA VASCONCELOS BRASIL, MARIANNA SAMPAIO SERPA, ANDREZA VERUSKA LIRA CORREIA, ELVIA CHRISTINA BARROS DE ALMEIDA, RODRIGO NEVES FLORÊNCIO, RODOLFO FROES CALIXTO, JUREMA FREIRE LISBOA DE CASTRO. FEDERAL UNIVERSITY OF PERNAMBUCO. Oral mucositis (OM) is a common unwanted effect of bone marrow transplantation (BMT). This study evaluated the effect of topical growth factors (TGF) associated with laser therapy in patients undergoing BMT with OM. Thirty-six of the 52 patients
OOOO February 2014 who received preventive laser treatment developed OM and were divided into two groups: treatment group (A), who received laser + TGF, and control group (B), who received laser + placebo. The average number of laser sessions and the average length of days for MO in placebo were higher than in the treatment group, respectively, 5.72 versus 4.89 and 7.28 versus 5.14. A statistically significant relationship was found between the number of days of use and MO TGF compared to placebo (p ¼ 0.019). The use of growth factors associated with topical laser therapy can be an alternative for treating oral mucositis.
PE-422 - LIFESTYLE HABITS AND CLEFT LIP AND PALATE: A POSSIBLE RELATIONSHIP? JAMILLE RIOS MOURA, RAYLENE LAÍSE SOUZA SILVA, BRUNO OLIVEIRA QUEIROZ, NILTON CÉSAR NOGUEIRA DOS SANTOS, VALÉRIA SOUZA FREITAS, MICHELLE MIRANDA LOPES FALCÃO. UNIVERSIDADE ESTADUAL DE FEIRA DE SANTANA. Cleft lips and palates are the commonest abnormalities in the craniofacial region. Their etiology is multifactorial and involves environmental and genetic factors. A systematic review of the literature was done to evaluate any relationship between tobacco and alcoholic drink use and cleft lips and palates. Materials and Methods: The PubMed database was searched. Eleven articles were selected, of which five dealt with the role of tobacco and seven the influence of alcohol on the development of clefts. Results: The quantity and type of drink and the mother’s and fetus’s capacity to metabolize alcohol were correlated with cleft development. Cigarette consumption by the parents and inhalation of smoke by the mother were also correlated. The parents’ habits of smoking and alcohol consumption compromised embryo development and fusion of the craniofacial processes. Conclusion: Tobacco and alcoholic drinks interfere with craniofacial development, thus increasing the risk of producing children with malformations.
PE-423 - LIPID DROPLETS IN ADENOID CYSTIC CARCINOMA WITH HIGH-GRADE TRANSFORMATION. HARIM TAVARES DOS SANTOS, VICTOR ANGELO MARTINS MONTALLI, FERNANDA VIVIANE MARIANO, ALBINA ALTEMANI. UNIVERSIDADE ESTADUAL DE CAMPINAS. This study sought to verify the quantity of cytoplasmic lipid droplets (CLDs) in adenoid cystic carcinoma with high-grade transformation (HGT-ACC) due to its association with tumor aggressiveness. Study Design: Six HGT-ACC samples were stained for adipophilin and Ki-67. Conventional and transformed areas were compared. The quantity of CLDs in tumor cells was classified as 0 (0%-5%), + (>5%-50%), or ++ (>50%). Results: In the conventional area 50% of the cases were + and 50% were 0. In the transformed area 83.3% of the cases were ++ and 17.7% were +. Ki-67 proliferation index was higher in the transformed area. Conclusion: In HGT-ACC the increased quantity of CLDs in the transformed areas probably reflects its higher lipogenesis due to tumor proliferation. FAPESP: 2012/ 18104-4.
PE-424 - LOW-LEVEL LASER THERAPY IN ORAL GRAFTVERSUS-HOST DISEASE. ELVIA CHRISTINA BARROS DE ALMEIDA, CATARINA DA MOTA VASCONCELOS BRASIL, MARIANNA SAMPAIO SERPA, ANDREZA VERUSKA LIRA CORREIA, MARIZE RAQUEL DINIZ DA ROSA, JUREMA
OOOO Volume 117, Number 2 FREIRE LISBOA DE CASTRO. FEDERAL UNIVERSITY OF PERNAMBUCO. Graft-versus-host disease (GVHD) is a major complication of allogeneic hematopoietic stem cell transplantation. Oral GVHD produces ulcerations, bleeding, lichenoid changes, atrophy, xerostomia, and sometimes pain. This study reported the treatment of oral GVHD in four cases with low-intensity laser therapy (LLLT). The wavelengths used were 660 nm (70 J/cm2) and 830 nm (35 J/cm2) for each lesion, with a 30-second exposure time per point. In half of the patients, GVHD was restricted to the oral cavity, and in half it manifested elsewhere, such as the lungs and eyes. The decrease in pain score over time was statistically significant, with a p-value of 0.033. The reduction in disease severity over time was statistically significant with a p-value of 0.012. The ease of using LLLT, its high acceptance by patients, and the positive results achieved make this therapy feasible for the treatment of oral GVHD.
PE-425 - MAST CELLS AND ANGIOGENESIS EVALUATION IN POTENTIALLY MALIGNANT LESIONS AND IN ORAL SQUAMOUS CELL CARCINOMA. CAROLINA RODRIGUES TEÓFILO, GALYLEIA MENESES CAVALCANTE, CAMILA CARVALHO DE OLIVEIRA, PAULO GOBERLÂNIO DE BARROS SILVA, ALCEU MACHADO DE SOUSA, CLARISSA PESSOA FERNANDES, ANA PAULA NEGREIROS NUNES ALVES. UNIVERSIDADE FEDERAL DO CEARÁ. Angiogenesis is essential for tumor growth by providing nutrition and oxygen to cells. Mast cells (MC) may be involved because they help regulate blood vessels. This study evaluated angiogenesis and MC density in oral epithelial dysplasia (ED) and in squamous cell carcinoma (SCC). The MC evaluation sample consisted of SCC (n ¼ 30), ED (n ¼ 23) and normal mucosa (NM) (n ¼ 20) as control. For angiogenesis the sample was 24 SCCs, 19 EDs, and 22 NMs. Immunohistochemical analysis was performed using anti-tryptase, anti-CD31, and anti-CD34. The MC sample showed lower density in SCC compared to NM and ED. In the angiogenesis sample, CD31 showed differences between SCC and ED, and SCC and NM, with a greater percentage of vessels in SCC, whereas CD34 showed no differences. The increase of vascularization in oral SCC suggests that angiogenesis is necessary for tumor growth. No correlation was found between MC and angiogenesis.
PE-426 - MAST CELL DEGRANULATION IN THE CHRONIC PERIODONTITIS OF NONLHIV-INFECTED AND HIV-INFECTED INDIVIDUALS UNDERGOING HIGHLY ACTIVE ANTIRETROVIRAL THERAPY. ALESSANDRO OLIVEIRA DE JESUS, ALESSANDRO OLIVEIRA DE JESUS, GIOVANNA RIBEIRO SOUTO, TAKESHI KATO SEGUNDO, PAÔLLA FREITAS PERDIGÃO, JULIANA MARIA BRAGA SCLAUSER BASÍLIO, RICARDO ALVES MESQUITA. FACULDADE DE ODONTOLOGIA - UNIVERSIDADE FEDERAL DE MINAS GERAIS. This study compared mast cell densities and percentage of intact and degranulated mast cells in chronic periodontitis of nonHIV-infected and HIV-infected individuals undergoing highly active antiretroviral therapy (HAART). Study Design: The gingival samples included 10 nonHIV-infected and 10 HIV-infected individuals who had chronic periodontitis. Samples were collected, processed, and stained with 0.3% toluidine
ABSTRACTS Abstracts e215 blue O. Densities of mast cells and percentages of intact and degranulated mast cells were obtained. Statistical analysis was accomplished using BioStatÒ. Results: HIV-infected samples had lower mast cell densities and a lower percentage of degranulated mast cells. There was no statistically significant difference between the groups evaluated. Conclusion: Mast cell densities and percentage of mast cell degranulation were not affected in individuals with HIV infection who were undergoing HAART. CNPq #309209/2010-2; 472045/2011-3; FAPEMIG.
PE-427 - MATRICARIA RECUTITA LINN. IN THE PREVENTION AND CONTROL OF RADIO-INDUCED ORAL MUCOSITIS. THAÍSE PEREIRA DANTAS SAMPAIO, TATIANA STUART VIEIRA HOLMES, DANÚBIA ROBERTA DE MEDEIROS NÓBREGA, MANUELA GOUVÊA CAMPÊLO DOS SANTOS, PATRÍCIA MEIRA BENTO, DALIANA QUEIROGA DE CASTRO GOMES, JOZINETE VIEIRA PEREIRA. UNIVERSIDADE ESTADUAL DA PARAÍBA. This study investigated the use of Matricaria recutita Linn. (chamomile) for the prevention and control of radiotherapyinduced oral mucositis (OM). Methods: This clinical, experimental, single-blinded trial included 22 patients divided into three groups, as follows: Group-I (n ¼ 7): 3% chamomile gel (CHAM) used from baseline until the end of radiotherapy; Group-II (n ¼ 7): 1% chlorhexidine gel used from the onset until OM regression; Group-III (n ¼ 8): CHAM used from the onset until OM regression. Data were analyzed descriptively and using the Kruskal-Wallis test (p 0.05). Results: No significant differences were noted between groups (p > 0.05) with respect to OM prevention and control. This study reported the effect of chamomile in decreasing OM severity and alleviating symptoms. Its antiinflammatory, antimicrobial, and healing activities mainly result from essential oils, flavonoids, and phenols. Conclusion: CHAM did not prevent the onset of OM, but effectively reduced its severity.
PE-428 - MMP-2 AND MMP-9 IMMUNOEXPRESSION IN TONGUE AND FLOOR OF MOUTH SQUAMOUS CELL CARCINOMA AT INITIAL CLINICAL STAGE. DANIELLE RESENDE CAMISASCA, LUISA AGUIRRE BUEXM, ELISA VIEIRA DIBLASI, PAULO ANTÔNIO SILVESTRE DE FARIA, SIMONE DE QUEIROZ CHAVES LOURENÇO, FERNANDO LUIZ DIAS. ONCOLOGY POST-GRADUATION PROGRAM - NATIONAL CANCER INSTITUTE (INCA), RIO DE JANEIROeBRAZIL. This study analyzed MMP-2 and MMP-9 immunostaining in patients with tongue and floor of mouth carcinomas (TFSCC) at clinical stages I-II. Study Design: Records from 156 patients were analyzed and submitted to bivariate (x2) and survival (Kaplan-Meier) analysis. Results: Men age 41 to 60 years who smoked and drank and developed tongue tumors clinical and pathological stages II were most often affected. Immunohistochemical analysis showed MMP-2 positivity in 98.1% of the cases and MMP-9 in 64.1% of the tumors. MMP-2 was mainly nuclear and cytoplasmic (76.9%); MMP-9 was predominantly cytoplasmic (48.1%). The worst disease-free survival was detected for patients whose tumors were at an advanced pathologic stage (p ¼ 0.006), poorly differentiated tumors (p ¼ 0.011), and those expressing MMP-2 (p ¼ 0.033).Conclusion: The demographic profile of patients with TFSCC in the initial clinical stage does not differ from the classic profile for