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Lymphoproliferative Disease in Mice
146:1, 2012
ESVP/ECVP Proceedings 2011
PORCINE ULCERATIVE DERMATITIS SYNDROME IN SOWS: POSSIBLE HERPES-ASSOCIATED ERYTHEMA MULTIFORME? M. R€ utten Vetsuisse-Faculty, Z€urich, Switzerland A 2-year-old German large white sow developed severe multifocal ulceration of the abdomen, udder and hind legs during pregnancy, birth and weaning of her piglets. The histological lesions consisted of lymphocytic interface dermatitis and folliculitis with vesicle formation at the epidermaledermal junction and multifocal epidermal necrosis and were interpreted as erythema multiforme. In addition, in the skin and in peripheral blood, leucocytes tested positive with a nested PCR for porcine lymphotropic herpesvirus 1. No evidence for porcine circovirus 2 could be found immunohistochemically. This is our third case of ulcerative dermatitis in sows in the last year that was positive for porcine lymphotropic herpesvirus 1. Attempted treatment with dexamethasone was in vain in these animals. These findings suggest that herpesvirus 1 could play a role in the development of erythema multiforme in pigs as is described for man and dogs. SPINAL CORD VISNA IN A SHEEP P. Pinczowski University of Zaragoza, Spain A sheep was presented to the veterinary hospital of the University of Zaragoza showing posterior ataxia. The animal was humanely destroyed and subjected to necropsy examination. There was an enlarged and heavy lung with miliary grey spots on the surface. The thoracic spinal cord showed light-brown discoloured areas in the right ventral horn. Microscopically, there was an intense gitter cell infiltration predominantly in the right ventral horn, associated with severe necrosis of the white and grey matter, intense astrogliosis and mononuclear perivascular cuffing. The brain exhibited less severe, but similar lesions, with multifocal distribution.
93
LYMPHOPROLIFERATIVE DISEASE IN MICE J. M. Ward Global VetPathology, Montgomery Village, Maryland and NIAID, NIH, Bethesda, Maryland, USA A 6-month-old male C3H mouse had generalized enlargement of lymph nodes and splenic enlargement. Histologically, there was loss of normal lymph node architecture and the node was composed of a monomorphic population of medium-sized lymphocytes which were CD3+ and CD45R+. The lymph node lesions appeared morphologically as lymphoma. The case, however, represented nonneoplastic, non-clonal lymphoproliferative disease due to a heritable mutation, Gld (generalized lymphoproliferative disease) involving Fasl, of the TNF receptor superfamily member 6 (synonyms - Tnfsf6, APT1LG1, CD178, CD95L). The enlarged nodes showed no evidence of clonal populations in Southern blotting. Later in life, however, the mice developed plasma cell tumours, which were clonal. A comparable disease occurs in man involving similar genes.
ESVP/ECVP Proceedings 2011
PORCINE ULCERATIVE DERMATITIS SYNDROME IN SOWS: POSSIBLE HERPES-ASSOCIATED ERYTHEMA MULTIFORME? M. R€ utten Vetsuisse-Faculty, Z€urich, Switzerland A 2-year-old German large white sow developed severe multifocal ulceration of the abdomen, udder and hind legs during pregnancy, birth and weaning of her piglets. The histological lesions consisted of lymphocytic interface dermatitis and folliculitis with vesicle formation at the epidermaledermal junction and multifocal epidermal necrosis and were interpreted as erythema multiforme. In addition, in the skin and in peripheral blood, leucocytes tested positive with a nested PCR for porcine lymphotropic herpesvirus 1. No evidence for porcine circovirus 2 could be found immunohistochemically. This is our third case of ulcerative dermatitis in sows in the last year that was positive for porcine lymphotropic herpesvirus 1. Attempted treatment with dexamethasone was in vain in these animals. These findings suggest that herpesvirus 1 could play a role in the development of erythema multiforme in pigs as is described for man and dogs. SPINAL CORD VISNA IN A SHEEP P. Pinczowski University of Zaragoza, Spain A sheep was presented to the veterinary hospital of the University of Zaragoza showing posterior ataxia. The animal was humanely destroyed and subjected to necropsy examination. There was an enlarged and heavy lung with miliary grey spots on the surface. The thoracic spinal cord showed light-brown discoloured areas in the right ventral horn. Microscopically, there was an intense gitter cell infiltration predominantly in the right ventral horn, associated with severe necrosis of the white and grey matter, intense astrogliosis and mononuclear perivascular cuffing. The brain exhibited less severe, but similar lesions, with multifocal distribution.
93
LYMPHOPROLIFERATIVE DISEASE IN MICE J. M. Ward Global VetPathology, Montgomery Village, Maryland and NIAID, NIH, Bethesda, Maryland, USA A 6-month-old male C3H mouse had generalized enlargement of lymph nodes and splenic enlargement. Histologically, there was loss of normal lymph node architecture and the node was composed of a monomorphic population of medium-sized lymphocytes which were CD3+ and CD45R+. The lymph node lesions appeared morphologically as lymphoma. The case, however, represented nonneoplastic, non-clonal lymphoproliferative disease due to a heritable mutation, Gld (generalized lymphoproliferative disease) involving Fasl, of the TNF receptor superfamily member 6 (synonyms - Tnfsf6, APT1LG1, CD178, CD95L). The enlarged nodes showed no evidence of clonal populations in Southern blotting. Later in life, however, the mice developed plasma cell tumours, which were clonal. A comparable disease occurs in man involving similar genes.