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M280 PREOPERATIVE CHEMO-BRACHYTHERAPY FOR LOCAL ADVANCED CERVICAL CANCER
Poster presentations / International Journal of Gynecology & Obstetrics 119S3 (2012) S531–S867
found feeling of disconfort like a sense of general malaise of the patient ended in the short time of observation. Conclusions: The surgical treatment with radio frequency in outpatient department. Is turned out particularly reliable as it has allowed a not invasive aimed suitable treatment in conditions of clinical emergency, good compliance of the patient to forehead of contained costs and nearly a total absence of complications. The persistence of displastic lesions, infection HPV, the appearance of recidivous has been observed in a limited percentage of cases. In the cases in which was necessary to carry out a successive treatment is held account of the anatomical characteristics of the portio previously dealt and desire or less of the woman than to conserve the fertility health. M277 RISK FACTORS ASSOCIATED WITH BREAST CANCER IN MEXICO G. Vega-Malagon ´ 1 , A.J. Vega-Malagon ´ 2 , A. Becerril-Santos2 , 2 J.A. Rodriguez-Mendez , H.L. Hernandez-Montiel2 , G. Saldivar-Lelo De Larrea2 , M. Vega-Malagon ´ 2 . 1 Coordinaci´ on de Educaci´ on M´edica, Universidad Aut´ onoma de Quer´etaro, Quer´etaro, Mexico; 2 Universidad Aut´ onoma de Quer´etaro, Quer´etaro, Mexico Objectives: To determine the risk factors associated with breast cancer in Mexico. Materials: Cases and controls. Methods: Cases and control factors. The cases were comprised of 60 women with breast cancer corroborated through histology. Control factors were 60 healthy women with mammograms that were negative for cancer. The socio-demographic, reproductive, anthropometric, preventive, medical background and toxicomania variables were analyzed. The analysis was carried out with an odds ratio, with a reliability interval of 95%, and with an x2 test, as well as a Student t. Results: The following factors showed a significant statistical difference (p < 0.05): Unmarried or with no constant partner (OR: 2.97, I.C. 1.29–6.83); no breastfeeding history (OR: 2.50, I.C. 1.05–0.05); IMC >25 (OR: 6.05, I.C. 2.53–14–47); abdominal perimeter >88 centimeters (OR: 2.58, I.C. 1.11–5.96): waist-hip index >0.86 (OR: 3.50, I.C. 1.55–7.87); never did self-exploratory breast examination (OR: 2.67, I.C. 1.17–6.03); self-exploratory breast examination more than 6 months before or never (OR: 3.76, I.C. 1.51–9.35); history of breast cancer in family (OR: 4.26, I.C. 1.12– 16.16); history of breast pathology (OR: 4.20, I.C. 1.12–16.16). Conclusions: The IMC, a family history of breast cancer, a history of breast pathology, the last breast self-examination being more than 6 months ago or never, the waist-hip index and the abdominal perimeter were the chief risk factors in breast cancer. It is important to know the main risk factors to make an early identification of women with high probabilities of cancer in order to offer them early detection and timely treatment. M278 Withdrawn M279 NOVEL OLIGOMANNOSE LIPOSOME-DNA COMPLEX DNA VACCINATION EFFICIENTLY EVOKES ANTI-HPV E6 AND E7 CTL RESPONSES T. Saito1 , M. Mizuuchi1 , Y. Hirohashi2 , T. Torigoe2 , N. Sato2 . 1 Obstetrics and Gynecology, Sapporo Medical University, Sapopro, Japan; 2 Department Pathology 1, Sapporo Medical University, Sapporo, Japan Objectives: The aim of this study was to establish an efficient human papilloma virus (HPV) type 16-targeting cancer immunotherapy. Persistent high-risk HPV infection causes cervical intra-epithelial neoplasia (CIN) and subsequent cervical carcinoma. HPV type16 (HPV16) is one of the common carcinogenic types and is found in about 50% of invasive cervical carcinomas. HPV16-derived
S621
viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. Thus, these proteins are regarded as ideal antigens for cervical carcinoma immunotherapy. Materials: In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). Methods: We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. Results: HPV16 E6-specific CTLs could be generated from HPV 16positive cervical carcinoma patient’s peripheral blood mononuclear cells (PBMCs) by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. Furthermore, we screened HLA-A24-restricted HPV16 E6- and E7derived peptides, and found that one E6-derived peptide (E6 66–74) showed the highest immunogenicity with ELISPOT assay from 100% of HPV16-positive patients (4 out of 4). On the other hand, other E6or E7-derived peptides, including E6 49–57, E6 82–90, E6 87–95, E6 98–106 and E7 83–93, showed less frequent reactivity. Conclusions: These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66–74, might be a suitable target of cervical cancer immunotherapy. M280 PREOPERATIVE CHEMO-BRACHYTHERAPY FOR LOCAL ADVANCED CERVICAL CANCER P. Qu1 , Y. Wei1 . 1 Tianjin Cencral Hospital for OB & GYN, Tianjin, China Objectives: To study the effect of surgery following platinumbased chemotherapy combined radiation therapy and concurrent chemoradiotherapy in treating cervical cancer of stage IB2-IIA. Materials: 101 cases cervical cancer of stage IB2-IIAwhich were all treated in our hospital during Jan. 2003 and Jul. 2010 were taken in the study. 59 patients in studying group, who were underwent surgery following chemotherapy one cycle and brachytherapy (2100 cGy-2400 cGy). 42 cases treated by concurrent chemoradiotherapy in control group. Methods: Short term therapic effect, adverse reaction and survival time between two groups were compared. Results: After chemotherapy combined brachytherapy 100% cases can be operated radically and 93.2% can reach pathological response in study group. There was no difference between these two groups in the effective rate after treatment. The 5 years-progression-free survival (PFS) rates were 84.3% and 92.8%, and 5 years-overall survival (OS) rates are 87.7% and 93.8% in study and control groups respectively by Kaplan-Meier. There was no difference in PFS and OS between two groups by log-rank test. Conclusions: The effect of surgery following chemotherapy combined brachytherapy is same as concurrent chemoradiotherpy in efficiency, while with less chance of adverse reaction. M281 LEEP EFFECTIVENESS IN GREEK PATIENTS WITH VAGINAL INTRAEPITHELIAL NEOPLASIA AND HISTORY OF CERVICAL CANCER D. Zygouris1 , G. Androutsopoulos2 , C. Grigoriadis1 , E. Terzakis1 . 1 St. Savvas Anticancer-Oncologic Hospital, Athens, Greece, Athens, Greece; 2 University of Patras, Medical School, Rion, Greece Objectives: Vaginal intraepithelial neoplasia is uncommon, representing 1% of lower genital tract intraepithelial neoplasia. In women with vaginal intraepithelial neoplasia: 78% may regress, 13% may persist and 9% may progress to invasive vaginal cancer. The aim of our study was to evaluate the therapeutic effectiveness of loop electrosurgical excision procedure (LEEP) in Greek patients with vaginal intraepithelial neoplasia and history of cervical cancer. Materials: Between January 2002 and January 2009, about 8 women with histologically confirmed vaginal intraepithelial
found feeling of disconfort like a sense of general malaise of the patient ended in the short time of observation. Conclusions: The surgical treatment with radio frequency in outpatient department. Is turned out particularly reliable as it has allowed a not invasive aimed suitable treatment in conditions of clinical emergency, good compliance of the patient to forehead of contained costs and nearly a total absence of complications. The persistence of displastic lesions, infection HPV, the appearance of recidivous has been observed in a limited percentage of cases. In the cases in which was necessary to carry out a successive treatment is held account of the anatomical characteristics of the portio previously dealt and desire or less of the woman than to conserve the fertility health. M277 RISK FACTORS ASSOCIATED WITH BREAST CANCER IN MEXICO G. Vega-Malagon ´ 1 , A.J. Vega-Malagon ´ 2 , A. Becerril-Santos2 , 2 J.A. Rodriguez-Mendez , H.L. Hernandez-Montiel2 , G. Saldivar-Lelo De Larrea2 , M. Vega-Malagon ´ 2 . 1 Coordinaci´ on de Educaci´ on M´edica, Universidad Aut´ onoma de Quer´etaro, Quer´etaro, Mexico; 2 Universidad Aut´ onoma de Quer´etaro, Quer´etaro, Mexico Objectives: To determine the risk factors associated with breast cancer in Mexico. Materials: Cases and controls. Methods: Cases and control factors. The cases were comprised of 60 women with breast cancer corroborated through histology. Control factors were 60 healthy women with mammograms that were negative for cancer. The socio-demographic, reproductive, anthropometric, preventive, medical background and toxicomania variables were analyzed. The analysis was carried out with an odds ratio, with a reliability interval of 95%, and with an x2 test, as well as a Student t. Results: The following factors showed a significant statistical difference (p < 0.05): Unmarried or with no constant partner (OR: 2.97, I.C. 1.29–6.83); no breastfeeding history (OR: 2.50, I.C. 1.05–0.05); IMC >25 (OR: 6.05, I.C. 2.53–14–47); abdominal perimeter >88 centimeters (OR: 2.58, I.C. 1.11–5.96): waist-hip index >0.86 (OR: 3.50, I.C. 1.55–7.87); never did self-exploratory breast examination (OR: 2.67, I.C. 1.17–6.03); self-exploratory breast examination more than 6 months before or never (OR: 3.76, I.C. 1.51–9.35); history of breast cancer in family (OR: 4.26, I.C. 1.12– 16.16); history of breast pathology (OR: 4.20, I.C. 1.12–16.16). Conclusions: The IMC, a family history of breast cancer, a history of breast pathology, the last breast self-examination being more than 6 months ago or never, the waist-hip index and the abdominal perimeter were the chief risk factors in breast cancer. It is important to know the main risk factors to make an early identification of women with high probabilities of cancer in order to offer them early detection and timely treatment. M278 Withdrawn M279 NOVEL OLIGOMANNOSE LIPOSOME-DNA COMPLEX DNA VACCINATION EFFICIENTLY EVOKES ANTI-HPV E6 AND E7 CTL RESPONSES T. Saito1 , M. Mizuuchi1 , Y. Hirohashi2 , T. Torigoe2 , N. Sato2 . 1 Obstetrics and Gynecology, Sapporo Medical University, Sapopro, Japan; 2 Department Pathology 1, Sapporo Medical University, Sapporo, Japan Objectives: The aim of this study was to establish an efficient human papilloma virus (HPV) type 16-targeting cancer immunotherapy. Persistent high-risk HPV infection causes cervical intra-epithelial neoplasia (CIN) and subsequent cervical carcinoma. HPV type16 (HPV16) is one of the common carcinogenic types and is found in about 50% of invasive cervical carcinomas. HPV16-derived
S621
viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. Thus, these proteins are regarded as ideal antigens for cervical carcinoma immunotherapy. Materials: In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). Methods: We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. Results: HPV16 E6-specific CTLs could be generated from HPV 16positive cervical carcinoma patient’s peripheral blood mononuclear cells (PBMCs) by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. Furthermore, we screened HLA-A24-restricted HPV16 E6- and E7derived peptides, and found that one E6-derived peptide (E6 66–74) showed the highest immunogenicity with ELISPOT assay from 100% of HPV16-positive patients (4 out of 4). On the other hand, other E6or E7-derived peptides, including E6 49–57, E6 82–90, E6 87–95, E6 98–106 and E7 83–93, showed less frequent reactivity. Conclusions: These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66–74, might be a suitable target of cervical cancer immunotherapy. M280 PREOPERATIVE CHEMO-BRACHYTHERAPY FOR LOCAL ADVANCED CERVICAL CANCER P. Qu1 , Y. Wei1 . 1 Tianjin Cencral Hospital for OB & GYN, Tianjin, China Objectives: To study the effect of surgery following platinumbased chemotherapy combined radiation therapy and concurrent chemoradiotherapy in treating cervical cancer of stage IB2-IIA. Materials: 101 cases cervical cancer of stage IB2-IIAwhich were all treated in our hospital during Jan. 2003 and Jul. 2010 were taken in the study. 59 patients in studying group, who were underwent surgery following chemotherapy one cycle and brachytherapy (2100 cGy-2400 cGy). 42 cases treated by concurrent chemoradiotherapy in control group. Methods: Short term therapic effect, adverse reaction and survival time between two groups were compared. Results: After chemotherapy combined brachytherapy 100% cases can be operated radically and 93.2% can reach pathological response in study group. There was no difference between these two groups in the effective rate after treatment. The 5 years-progression-free survival (PFS) rates were 84.3% and 92.8%, and 5 years-overall survival (OS) rates are 87.7% and 93.8% in study and control groups respectively by Kaplan-Meier. There was no difference in PFS and OS between two groups by log-rank test. Conclusions: The effect of surgery following chemotherapy combined brachytherapy is same as concurrent chemoradiotherpy in efficiency, while with less chance of adverse reaction. M281 LEEP EFFECTIVENESS IN GREEK PATIENTS WITH VAGINAL INTRAEPITHELIAL NEOPLASIA AND HISTORY OF CERVICAL CANCER D. Zygouris1 , G. Androutsopoulos2 , C. Grigoriadis1 , E. Terzakis1 . 1 St. Savvas Anticancer-Oncologic Hospital, Athens, Greece, Athens, Greece; 2 University of Patras, Medical School, Rion, Greece Objectives: Vaginal intraepithelial neoplasia is uncommon, representing 1% of lower genital tract intraepithelial neoplasia. In women with vaginal intraepithelial neoplasia: 78% may regress, 13% may persist and 9% may progress to invasive vaginal cancer. The aim of our study was to evaluate the therapeutic effectiveness of loop electrosurgical excision procedure (LEEP) in Greek patients with vaginal intraepithelial neoplasia and history of cervical cancer. Materials: Between January 2002 and January 2009, about 8 women with histologically confirmed vaginal intraepithelial