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M307 YOUNG WOMEN'S BREAST CANCER
S630
Poster presentations / International Journal of Gynecology & Obstetrics 119S3 (2012) S531–S867
M306 COEXPRESSION OF CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR AND NUCLEAR FACTOR-úB SIGNALING PATHWAY IN HUMAN CERVICAL CANCER PROGRESSION L. Hu1 . 1 The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Objectives: The overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) and transcription factor NF-úB activation was involved in cervical cancer progression has been reported previously. To evaluate the potential cross-talk between CFTR and NF-úB pathway in cervical cancer, we examined the related proteins simultaneously in human cervical cancer tissues and cell lines. Materials: 135 cervical tissue samples including normal, precancerous and cancerous tissues were analyzed. Methods: The expressions of CFTR and NF-úB pathway proteins were analyzed respectively by immunohistochemistry and Western blot in total of 135 cervical tissue samples including normal, precancerous and cancerous tissues. Dual immunofluorescent analysis was performed to detect the coexpression of CFTR and NF-úB in cervical cancer tissues and cell lines. The correlation between clinical features and CFTR/NF-úB pathway expression was analyzed. Results: The membrane expression of CFTR and nuclear translocation of NF-úB was parallel increased with the decrease of IúB-a protein in cytosol from normal cervical tissue, cervical intraepithelial neoplasm (CIN) to cervical carcinoma. The similarly coexpression was also observed in cervical cell lines. Further, CFTR expression/NF-úB activation increased along with the depth of invasion and tumor grade (P < 0.05). Conclusions: CFTR and NF-úB pathway was coexpression in cervical cancer. The cross-talk of CFTR and NF-úB pathway may participate in the cervical carcinogenesis with CFTR as a potential regulator. M307 YOUNG WOMEN’S BREAST CANCER O. Khelifi1 , D. Zeghal1 , S. Jeridi1 , R. Ben hmid1 , F. Zouari1 , S. Mahjoub1 . 1 C, Maternity Center of Tunis, Tunis, Tunisia Objectives: The aim of this study is to specify diagnostic and therapeutic features of young women’s breast cancer to establish guidelines to improve the prognosis of this disease. Materials: Our study is a retrospective one dealing with 32 patients aged less than 40 years old having the diagnosis of breast cancer. Methods: The study was held from 2005 to 2011 in the Department “C” of Gynecology and Obstetrics in the Center of maternity and neonatology of Tunis. Results: Patients intend outpatient clinic mainly when women feel a lump different from the rest of the breast tissue. The average tumor size was 34 mm. Ductal infiltring carcinoma was the most frequent histological type with predominance of SBR III grade. Surgical treatment was radical in 75% of the cases because of predominant advanced stage cancers. Lymph node invasion was noted in 77, 4% of operated patients. Local radiation was indicated in 90, 6% of the cases, chemotherapy in 87, 7% and first-line hormonal therapy in 53%. The survival rate at 4 and 5 years was 80, 2% and 53, 5% respectively. The most important prognostic factors were: the delay for consultation (more than 3 months) and the histological characters of the tumors: lymph node invasion with capsular burglary and negative hormonal receptors in most cases. Conclusions: The analysis of our results and the medical database allowed us to improve our knowledge about the breast cancer of young women in Tunisia and enabled us to establish guidelines to prevent and improve the prognosis of this disease.
M308 ACCURACY OF INTRAOPERATIVE FROZEN SECTION IN THE DIAGNOSIS OF OVARIAN TUMORS F. Yarandi1 , Z. Eftekhar1 , H. Shojaee2 , F. Haghi2 , M. Hojjati Najafabadi3 . 1 Gynecological Oncology, Medical Sciences/University of Tehran, Tehran, Iran, Islamic Republic of ; 2 University of Tehran, Teharan, Iran, Islamic Republic of ; 3 Azad University, Teharan, Iran, Islamic Republic of Objectives: Ovarian neoplasms are an important cause of morbidity and mortality in women. The surgical management of ovarian neoplasms depends on their correct categorisation as benign, borderline and malignant. The aim of this study was to determine the clinical benefits of intraoperative frozen section analysis into the surgical management policy of women referred with an adnexal mass suspicious of ovarian cancer. Materials: 106 women were examined at oncology department of women hospital during 10 years. Methods: A retrospective study of 106 ovarian frozen section results was examined to determine the accuracy of frozen section diagnosis. The accuracy, sensitivity, specificity, and positive and negative predictive value of frozen section were studied. Results: The overall accuracy to determine the status of malignancy was 93.3%. Sensitivity of the test was highest in the benign groups at 97.4% and lowest in the borderline groups at 25%. The accuracy of frozen section was 80% in serous tumours and 60% in mucinous. There were two (2.5%) false positive, three (10.7%) false negative and two overestimated diagnosis in frozen section examination. Eight malignancies (30.7%) were of metastatic origin, all of which (100%), were correctly identified on frozen section. Conclusions: Frozen section appears to be an accurate technique for the histopathological diagnosis of ovarian tumours. Some limitations were observed among borderline and mucinous tumours. This emphasises the great value of frozen section in the diagnosis of ovarian tumours. M309 IMPORTANCE LIPID HYDROPEROXIDES IN THE NORMAL TISSUE OF ENDOMETRIUM AND ENDOMETRIUM CARCINOMA N. Lucic1 , Z. Antonic1 , V. Ecim1 , S. Grahovac1 . 1 Gynecology Obstretics, University-Clinical Centre Banja Luka, Banja Luka, Bosnia and Herzegovina Objectives: Many experimental study show involvement of oxygen derived radicals in the pathogenesis of gynecologycal disoders, as well in cancer development. The objective of the present study was to examine changes in activites and levels lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed in the normal tissue of the endometrijum and endometrium carcinoma. Materials: The study has been carried out on 70 patients. We have analysed: age patients, the supersonic test – transvaginal probe, pathohistological diagnosis analysis (PHD) of the curet of the patient – we have gathered the tissue of the normal and the pathologicaly changed endometrium from the exploratory curretage, determining LOOH in the blood and endometrial tissue of the normal and pathological endometrium of the uterus. Methods: The Group A has been made out of 30 of them who did not have the irregular bleeding from the uterus, and 40 of the represented the Group B with the irregular bleeding, who also had PHD confirmed malign changes of the endometrium. Results: Dominant age in the Group A i B is 41–50 (59%). LOOH level in blood was observed in patients with normal tissure of the endometrium (29%), where as it was significantly higher in endometrium carcinoma (61%). LOOH level in endometrium tissue was significantly increased in patients with carcinoma endometrium (89%), compared to normal tissure of the endometrium (34%). Conclusions: Our findings suggest that the increase in LOOH level, in patients with gynecological disorders render these patients more
Poster presentations / International Journal of Gynecology & Obstetrics 119S3 (2012) S531–S867
M306 COEXPRESSION OF CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR AND NUCLEAR FACTOR-úB SIGNALING PATHWAY IN HUMAN CERVICAL CANCER PROGRESSION L. Hu1 . 1 The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China Objectives: The overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) and transcription factor NF-úB activation was involved in cervical cancer progression has been reported previously. To evaluate the potential cross-talk between CFTR and NF-úB pathway in cervical cancer, we examined the related proteins simultaneously in human cervical cancer tissues and cell lines. Materials: 135 cervical tissue samples including normal, precancerous and cancerous tissues were analyzed. Methods: The expressions of CFTR and NF-úB pathway proteins were analyzed respectively by immunohistochemistry and Western blot in total of 135 cervical tissue samples including normal, precancerous and cancerous tissues. Dual immunofluorescent analysis was performed to detect the coexpression of CFTR and NF-úB in cervical cancer tissues and cell lines. The correlation between clinical features and CFTR/NF-úB pathway expression was analyzed. Results: The membrane expression of CFTR and nuclear translocation of NF-úB was parallel increased with the decrease of IúB-a protein in cytosol from normal cervical tissue, cervical intraepithelial neoplasm (CIN) to cervical carcinoma. The similarly coexpression was also observed in cervical cell lines. Further, CFTR expression/NF-úB activation increased along with the depth of invasion and tumor grade (P < 0.05). Conclusions: CFTR and NF-úB pathway was coexpression in cervical cancer. The cross-talk of CFTR and NF-úB pathway may participate in the cervical carcinogenesis with CFTR as a potential regulator. M307 YOUNG WOMEN’S BREAST CANCER O. Khelifi1 , D. Zeghal1 , S. Jeridi1 , R. Ben hmid1 , F. Zouari1 , S. Mahjoub1 . 1 C, Maternity Center of Tunis, Tunis, Tunisia Objectives: The aim of this study is to specify diagnostic and therapeutic features of young women’s breast cancer to establish guidelines to improve the prognosis of this disease. Materials: Our study is a retrospective one dealing with 32 patients aged less than 40 years old having the diagnosis of breast cancer. Methods: The study was held from 2005 to 2011 in the Department “C” of Gynecology and Obstetrics in the Center of maternity and neonatology of Tunis. Results: Patients intend outpatient clinic mainly when women feel a lump different from the rest of the breast tissue. The average tumor size was 34 mm. Ductal infiltring carcinoma was the most frequent histological type with predominance of SBR III grade. Surgical treatment was radical in 75% of the cases because of predominant advanced stage cancers. Lymph node invasion was noted in 77, 4% of operated patients. Local radiation was indicated in 90, 6% of the cases, chemotherapy in 87, 7% and first-line hormonal therapy in 53%. The survival rate at 4 and 5 years was 80, 2% and 53, 5% respectively. The most important prognostic factors were: the delay for consultation (more than 3 months) and the histological characters of the tumors: lymph node invasion with capsular burglary and negative hormonal receptors in most cases. Conclusions: The analysis of our results and the medical database allowed us to improve our knowledge about the breast cancer of young women in Tunisia and enabled us to establish guidelines to prevent and improve the prognosis of this disease.
M308 ACCURACY OF INTRAOPERATIVE FROZEN SECTION IN THE DIAGNOSIS OF OVARIAN TUMORS F. Yarandi1 , Z. Eftekhar1 , H. Shojaee2 , F. Haghi2 , M. Hojjati Najafabadi3 . 1 Gynecological Oncology, Medical Sciences/University of Tehran, Tehran, Iran, Islamic Republic of ; 2 University of Tehran, Teharan, Iran, Islamic Republic of ; 3 Azad University, Teharan, Iran, Islamic Republic of Objectives: Ovarian neoplasms are an important cause of morbidity and mortality in women. The surgical management of ovarian neoplasms depends on their correct categorisation as benign, borderline and malignant. The aim of this study was to determine the clinical benefits of intraoperative frozen section analysis into the surgical management policy of women referred with an adnexal mass suspicious of ovarian cancer. Materials: 106 women were examined at oncology department of women hospital during 10 years. Methods: A retrospective study of 106 ovarian frozen section results was examined to determine the accuracy of frozen section diagnosis. The accuracy, sensitivity, specificity, and positive and negative predictive value of frozen section were studied. Results: The overall accuracy to determine the status of malignancy was 93.3%. Sensitivity of the test was highest in the benign groups at 97.4% and lowest in the borderline groups at 25%. The accuracy of frozen section was 80% in serous tumours and 60% in mucinous. There were two (2.5%) false positive, three (10.7%) false negative and two overestimated diagnosis in frozen section examination. Eight malignancies (30.7%) were of metastatic origin, all of which (100%), were correctly identified on frozen section. Conclusions: Frozen section appears to be an accurate technique for the histopathological diagnosis of ovarian tumours. Some limitations were observed among borderline and mucinous tumours. This emphasises the great value of frozen section in the diagnosis of ovarian tumours. M309 IMPORTANCE LIPID HYDROPEROXIDES IN THE NORMAL TISSUE OF ENDOMETRIUM AND ENDOMETRIUM CARCINOMA N. Lucic1 , Z. Antonic1 , V. Ecim1 , S. Grahovac1 . 1 Gynecology Obstretics, University-Clinical Centre Banja Luka, Banja Luka, Bosnia and Herzegovina Objectives: Many experimental study show involvement of oxygen derived radicals in the pathogenesis of gynecologycal disoders, as well in cancer development. The objective of the present study was to examine changes in activites and levels lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed in the normal tissue of the endometrijum and endometrium carcinoma. Materials: The study has been carried out on 70 patients. We have analysed: age patients, the supersonic test – transvaginal probe, pathohistological diagnosis analysis (PHD) of the curet of the patient – we have gathered the tissue of the normal and the pathologicaly changed endometrium from the exploratory curretage, determining LOOH in the blood and endometrial tissue of the normal and pathological endometrium of the uterus. Methods: The Group A has been made out of 30 of them who did not have the irregular bleeding from the uterus, and 40 of the represented the Group B with the irregular bleeding, who also had PHD confirmed malign changes of the endometrium. Results: Dominant age in the Group A i B is 41–50 (59%). LOOH level in blood was observed in patients with normal tissure of the endometrium (29%), where as it was significantly higher in endometrium carcinoma (61%). LOOH level in endometrium tissue was significantly increased in patients with carcinoma endometrium (89%), compared to normal tissure of the endometrium (34%). Conclusions: Our findings suggest that the increase in LOOH level, in patients with gynecological disorders render these patients more