mouse hybrid myotubes

mouse hybrid myotubes

Brief Communication 855 Mammalian postmitotic nuclei reenter the cell cycle after serum stimulation in newt/mouse hybrid myotubes Cristiana P. Vellos...

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Brief Communication 855

Mammalian postmitotic nuclei reenter the cell cycle after serum stimulation in newt/mouse hybrid myotubes Cristiana P. Velloso, Andra´s Simon and Jeremy P. Brockes Cell cycle reentry and dedifferentiation of postmitotic cells are important aspects of the ability of an adult newt and other urodele amphibians to regenerate various tissues and appendages [1]. In contrast to their mammalian counterparts, newt A1 myotubes are able to reenter S phase after serum stimulation of a pathway leading to phosphorylation of the retinoblastoma protein, pRb [2]. The activity in serum is not due to mitogenic growth factors but is generated indirectly by the activation of thrombin and subsequent proteolysis [3]. In this paper we describe the formation of interspecies hybrid (heterokaryon) myotubes by the fusion of mouse C2C12 [4] and newt A1 [5, 6] myogenic cells. The C2C12 nuclei reenter the cell cycle upon serum stimulation of the hybrids, while C2C12 homokaryon myotubes remain arrested under these conditions. These findings indicate that the postmitotic arrest of the mouse nuclei is undermined by the pathway activated in the newt cytoplasm. The hybrid myotubes provide a new model for the manipulation of the postmitotic arrest in both mammalian and newt differentiated cells.

[6]. Appendage regeneration in the newt proceeds by the formation of a blastema, a mesenchymal growth zone of undifferentiated, proliferating cells that is largely derived by local dedifferentiation of the stump tissues [1]. After implantation into a blastema, A1 myotubes are able both to reenter the cell cycle and to give rise to mononucleate, cycling progeny cells that contribute to the blastema [9, 10]. Mammalian myotubes do not exhibit comparable plasticity, although reentry to S phase can be achieved by manipulations that inactivate the Rb gene or protein [11–13], while the multinucleate-to-mononucleate transition can be induced by the expression of the msx-1 homeobox gene or by exposure to the substituted purine called myoseverin [14, 15].

Results and discussion

Newt myotubes are induced to reenter S phase when they are stimulated by serum from various mammalian sources [2]. Thus, the extracellular milieu provided by mammalian serum induces urodele cell cycle reentry, but for some reason mouse myotubes remain in arrest. This may be an interesting cellular model for the loss of widespread regenerative ability in the mammal [3]. In order to determine if mouse nuclei are able to respond to the intracellular events induced by the serum pathway in newt cells, we generated interspecies hybrid myotubes. Mononucleate C2C12 and A1 cells were mixed and cultured at 33⬚C, a temperature between the optima for amphibian and mammalian cells. In appropriate medium (see Materials and methods), both precursor cells were able to proliferate and form myotubes. After waiting 4 days to allow fusion, we trypsinized, partially purified, and replated the myotubes. The final preparation was a mixture of mononucleate cells and myotubes, and 10%–20% of the latter were identified as hybrids in different experiments. Such cultures were either maintained in medium containing 0.5% foetal bovine serum (FBS) or shifted to 15% FBS for 5 days. The nucleotide analog 5-bromo-2deoxyuridine (BrdU) was added for the last 48 hr. Cells were fixed and processed for immunocytochemistry, and we assayed S phase reentry by counting nuclei that had incorporated BrdU (see Materials and methods).

Myogenic differentiation involves the withdrawal of mononucleate precursor cells from the cell cycle and their fusion into multinucleate myotubes. One can achieve this in culture by lowering the serum concentration in the medium and allowing the formation of postmitotic myotubes [7, 8]. Such myotubes are arrested in that they are refractory to mitogens that act on their precursor cells. Myotubes of the newt, however, have a remarkable capacity to reenter S phase, both in culture after serum stimulation [2] and after implantation into a regenerating limb

The phase contrast image in Figure 1a shows a typical hybrid myotube with seven nuclei. In order to distinguish the C2C12 and A1 nuclei, we used the species-specific XB10 antibody [16], which recognizes the mouse nuclear envelope proteins lamin A and C (Figure 1b). Hybrid myotubes were found to express myosin heavy chain (MHC), a marker of late differentiation (Figure 1c). After stimulation in medium containing 0.5% FBS, less than 0.6% of the homokaryon or heterokaryon myotubes con-

Address: Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom. Correspondence: Jeremy P. Brockes E-mail: [email protected] Received: 6 March 2001 Revised: 6 April 2001 Accepted: 6 April 2001 Published: 5 June 2001 Current Biology 2001, 11:855–858 0960-9822/01/$ – see front matter  2001 Elsevier Science Ltd. All rights reserved.

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Figure 1

Identification of newt/mouse hybrid myotubes. (a) Phase contrast image of a hybrid myotube lying in the vicinity of A1 (white arrowhead) and C2C12 (white arrow) mononucleate cells. The myotube contains two mouse nuclei, which are marked with black arrows. (b) The MHC staining is in blue, and lamin staining is in red. Only the mouse nuclei are stained for lamin, and the A1 nuclei are negative both in

the myotube and in the nearby mononucleate cell. (c) The MHC staining in blue shows the contours of the nuclei in the syncytium. The blue signal in the nearby BrdU-positive C2C12 mononucleate cell (arrow) is a result of the overlapping emission spectra of the secondary antibodies used for MHC and BrdU staining. Note that the BrdU incorporation result is not shown. The scale bar is 50 ␮m.

tained BrdU-positive nuclei, while 15.3% of the mononucleate A1 and 94.4% of C2C12 cells were cycling (Table 1). This indicates that both classes of myotube were resistant to mitogens acting on their precursor cells and hence that the postmitotic arrest was intact.

which only the A1 or both the A1 and C2C12 nuclei incorporated BrdU (Figure 2d–f). It should be noted that the response of A1 homokaryon myotubes to serum was lower at the intermediate temperature than was that observed at 25⬚C ([2] and data not shown).

The myotubes were purified and shifted to medium with 15% FBS. The percentage of BrdU-positive C2C12 homokaryon myotubes did not increase in high serum, whereas the number of A1 homokaryon myotubes with BrdUpositive nuclei was elevated to 3.9%, in agreement with earlier findings [2]. We observed that 9.8% of hybrid myotubes contained C2C12 nuclei that had reentered S phase. The BrdU-positive hybrids fell into three different categories (Table 1); in most cases, only C2C12 nuclei were labeled (Figure 2a–c), but we also found examples in

We investigated if S phase reentry of mouse nuclei in the hybrid myotubes could be initiated by the thrombinderived signal that was previously found to stimulate A1 homokaryon myotubes, but not A1 mononucleate cells, at 25⬚C [3]. The identity of this factor is currently unknown, but it is present in crude preparations of bovine thrombin and has been shown to be distinct from but activated by the protease [3]. Therefore, S phase entry was assayed in the presence of 0.5% FBS and crude thrombin. The A1 mononucleate cells were not responsive to

Table 1 S phase entry in myotubes and mononucleate cells. A2 myotubes

Hybrid myotubes

C2C12 myotubes

Number labeled with BrdU

Total number analyzed

Percent labeled

Number labeled with BrdU C2 only

A1 only

C2 and A1

Total number analyzed

0.5% FBS 15% FBS Thrombin

2 121 8

1083 3080 592

0.2 3.9 1.4

2 (0.6%) 43 (6.8%) 18 (10.1%)

0 2 (0.3%) 4 (2.2%)

0 19 (3.0%) 4 (2.8%)

351 630 178

0.5% FBS 15% FBS Thrombin

52 90 52

A1 mononucleate cells 340 279 328

15.3 32.3 15.9

Number labeled with BrdU 1 6 3

Total number analyzed

Percent labeled

576 3635 522

0.2 0.2 0.6

C2C12 mononucleate cells 240 321 198

254 324 226

94.4 99.1 87.6

This includes the cumulative data from 11 independent experiments in which we matched the response of the heterokaryon myotubes to high serum or thrombin stimulation to control myotubes in low serum. All categories of cells were counted in the same tissue culture dishes except for the C2C12 homokaryon myotubes, which were analyzed separately.

Brief Communication 857

Figure 2

favorable for reentry by the mammalian than by the newt nuclei, thus curtailing the response of the A1 nuclei. The relatively low response of the A1 homokaryons at 33⬚C is consistent with this hypothesis. Alternatively, the different nuclei may reenter the cell cycle asynchronously, with the C2C12 nuclei entering S phase first. Such a phenomenon could be enhanced by a lower threshold for this response in the C2C12 as compared to the A1 nuclei. Muscle heterokaryons have proven to be informative in the study of a variety of cellular processes [17–19]. Here we present a newt/mouse hybrid myotube model in which the corresponding homokaryons differ in their ability to countermand the postmitotic arrest and reenter S phase. Cell cycle reentry in A1 homokaryons is mediated by the phosphorylation of pRb [2], and it seems plausible that mouse nuclei in the hybrids also respond to this pathway. In earlier experiments, mouse Rb was expressed in newt myotubes, and acted to reinforce the arrest [2]. The response of mammalian nuclei in the hybrids encourages further experiments that aim to identify the newt factors that induce cell cycle reentry. Mammalian nuclei can be reprogrammed after transplantation to oocyte cytoplasm [20], but the present experiments demonstrate that a more selective aspect of plasticity can also be imposed on postmitotic nuclei.

Materials and methods Generation of hybrid myotubes Serum stimulates the S phase entry of both A1 and C2C12 nuclei in a hybrid myotube. (a) Phase contrast image of a hybrid myotube with eight A1 and two C2C12 nuclei (arrows). (b) The MHC staining is in blue, and BrdU staining is in turquoise. The BrdU-positive C2C12 nucleus is marked with an arrow. (c) The MHC staining is in blue, and lamin staining is in red. The C2C12 nuclei are laminpositive and one has incorporated BrdU, while none of the A1 nuclei have labeled. (d) Phase contrast image of a hybrid myotube with two A1 (arrowheads) and two C2C12 nuclei (arrows). (e) The MHC staining is in blue, and BrdU staining is in turquoise. The A1 nuclei are marked with arrowheads, and C2C12 nuclei are marked with arrows. (f) The MHC staining is in blue, and lamin staining is in red. Both lamin-positive C2C12 nuclei have incorporated BrdU. The weak, punctate labeling in the A1 nuclei that we occasionally observed is probably due to the transfer of the mouse antigen between the two types of nuclei. The scale bar is 50 ␮m.

A1 and C2C12 mononucleate cells were maintained as described [2]. Cycling A1 cells were plated on 75 cm2 scored gelatin-coated dishes [2]. At 90% confluency, the amphibian medium was changed to Dulbecco’s Modified Eagle Medium (DMEM; Gibco) containing 0.5% FBS (First Link) at 33⬚C, and cells were grown in the presence of 10% CO2. After 1 day, C2C12 cells were added to the A1 cells at a 4:1 A1:C2C12 ratio. After 4 days of fusion, the hybrids were purified as described for A1 homokaryons [2] and plated on fibronectin-coated dishes.

S phase reentry assay The purified myotubes were cultured in DMEM containing 0.5% FBS or shifted either to DMEM containing 15% FBS or to DMEM containing 0.5% FBS and 100 ␮g ml⫺1 crude thrombin (Calbiochem) [3]. After 3 days, BrdU was added to a final concentration of 10 mM. After 5 days, cells were washed once in PBS and fixed in 2% paraformaldehyde in PBS for 20 s, then postfixed in 100% ice-cold methanol for 5 min.

Immunocytochemistry and image analysis

crude thrombin as expected [3], but 1.4% of the A1 homokaryon myotubes were induced to reenter the cell cycle. Importantly, in 12.9% of the hybrid myotubes the C2C12 nuclei reentered S phase, either exclusively or in conjunction with A1 nuclei (Table 1). These experiments indicate that postmitotic mouse C2C12 nuclei were responsive to activation of the newt pathway in the interspecies heterokaryons.

BrdU and MHC staining were performed as described [2]. BrdU staining was visualized with a fluorescein-conjugated IgG1-specific secondary antibody (Southern Biotechnology Associates), and MHC staining was visualized with a biotin-conjugated secondary antibody (Dako) and cascade blue-conjugated streptavidin (Molecular Probes). To visualize laminstaining by the XB10 antibody, we used IgM-specific, texas red-conjugated secondary antibody (Southern Biotechnology Associates). Cells were observed under a Zeiss Axiophot 2 microscope. Images were captured on a color CCD camera (Japanese Video Company) and were color-corrected with Image-Pro-Plus software (Media Cybernetics).

Acknowledgements It was surprising that C2C12 nuclei were more often BrdU-positive in the hybrids than their A1 counterparts. It is possible that the temperature of 33⬚C may be more

We thank A. Gann, who isolated the first hybrids, and E. Tanaka for much helpful discussion. This work was supported by a fellowship from CAPES (Brazil) to C.P.V, a postdoctoral fellowship from the Wenner-Gren Foundation to A.S., and an MRC Program Grant to J.P.B.

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References 1. Brockes JP: Amphibian limb regeneration: rebuilding a complex structure. Science 1997, 276:81-87. 2. Tanaka EM, Gann AA, Gates PB, Brockes JP: Newt myotubes reenter the cell cycle by phosphorylation of the retinoblastoma protein. J Cell Biol 1997, 136:155-165. 3. Tanaka EM, Drechsel DN, Brockes JP: Thrombin regulates S-phase re-entry by cultured newt myotubes. Curr Biol 1999, 9:792-799. 4. Yaffe D, Saxel O: Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 1977, 270:725-727. 5. Ferretti P, Brockes JP: Culture of newt cells from different tissues and their expression of a regeneration-associated antigen. J Exp Zool 1988, 247:77-91. 6. Lo DC, Allen F, Brockes JP: Reversal of muscle differentiation during urodele limb regeneration. Proc Natl Acad Sci USA 1993, 90:7230-7234. 7. Lassar AB, Skapek SX, Novitch B: Regulatory mechanisms that coordinate skeletal muscle differentiation and cell cycle withdrawal. Curr Opin Cell Biol 1994, 6:788-794. 8. Walsh K, Perlman H: Cell cycle exit upon myogenic differentiation. Curr Opin Genet Dev 1997, 7:597-602. 9. Kumar A, Velloso CP, Imokawa Y, Brockes JP: Plasticity of retrovirus-labelled myotubes in the newt limb regeneration blastema. Dev Biol 2000, 218:125-136. 10. Velloso CP, Kumar A, Tanaka EM, Brockes JP: Generation of mononucleate cells from post-mitotic myotubes proceeds in the absence of cell cycle progression. Differentiation 2000, 66:239-246. 11. Endo T, Goto S: Retinoblastoma gene product Rb accumulates during myogenic differentiation and is deinduced by the expression of SV40 large T antigen. J Biochem 1992, 112:427430. 12. Crescenzi M, Soddu S, Tato F: Mitotic cycle reactivation in terminally differentiated cells by adenovirus infection. J Cell Physiol 1995, 162:26-35. 13. Schneider JW, Gu W, Zhu L, Mahdavi V, Nadal-Ginard B: Reversal of terminal differentiation mediated by p107 in Rb-/- muscle cells. Science 1994, 264:1467-1471. 14. Rosania GR, Chang YT, Perez O, Sutherlin D, Dong H, Lockhart DJ, et al.: Myoseverin, a microtubule-binding molecule with novel cellular effects. Nat Biotechnol 2000, 18:304-308. 15. Odelberg SJ, Kollhoff A, Keating MT: Dedifferentiation of mammalian myotubes induced by msx1. Cell 2000, 103:1099-1109. 16. Horton H, McMorrow I, Burke B: Independent expression and assembly properties of heterologous lamins A and C in murine embryonal carcinomas. Eur J Cell Biol 1992, 57:172183. 17. Blau HM, Chiu CP, Webster C: Cytoplasmic activation of human nuclear genes in stable heterocaryons. Cell 1983, 32:11711180. 18. Wright WE: Control of differentiation in heterokaryons and hybrids involving differentiation-defective myoblast variants. J Cell Biol 1984, 98:436-443. 19. Mook-Jung I, Gordon H: Acetylcholine receptor clustering associates with proteoglycan biosynthesis in C2 variant and heterkaryon muscle cells. J Neurobiol 1996, 31:210-218. 20. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH: Viable offspring derived from fetal and adult mammalian cells. Nature 1997, 385:810-813.