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Management of lung cancer in a general hospital in Spain. Role of a lung cancer committee
52 195 PRIMARY REPORT: A NE\!IlID OF I~iOLCUL~ EPIDEIJIOLWGY FOR ::O;lAN'S LUliGCA"C1R IN CBINA Ruei-jiang Xie Beijing TB & Thorncic Tumor Inst. The sex ratio reveals there is a hioh proportion of Chinese woman on lung cancer (LCY; and the factors of LC are always belonged to coal smoke pollution pattern. So, study on the relationship h tween dose and effective of BPDE-DKA adduct (BD A e in excised lung tumour is undertaken. ::ethods:1) Case control study is used, then four groups (Gs) are established (male (M) 19 pairs & female (F) 18 pairs). Objects are selected from the lung disease cases whose lesions are excised. 2) Two questionnaires are filled for every object, 3) Every lung tissue must be passed through pathology examination. 4) %’ nucleotide post labeling and chromatography, synchronous scanning fluorescent spectrophotometry,mass spectrometry, and immunoassays are applied for measuring BDA. Results: 1) There is significant difference (S.D) ;;ot;; quantity of BDA betwsen F's 2 Gs, experiexp) 1.56 (RAL In10 ) to controlled (con) 0.79, t=2.84, Ta3.025. However, there is no S.3 between M's 2 Gs (2.01 to 2.06). 2) Two LC Gs (F 3) to M) have not been found S.D (t=1.61 PW.1). F smoker's BDA is higher than none smoker (2.26 to 1.01 t=2.56 P-0.025); but it is very similar 4) It with M smoker (2.26 to 2.06 t=0.29 PW.5). is difficult to find any S.D between F passive smoking (ps), excluding smoker, such as exp to con, or no ps to ps in F LC G. Conclusion: BPDE-DKA adduct could become as an index for woman's lung cancer in future.
MANAGEMENT OF WNG CANCER IN A GENERAL HO!%ITAL ROLE OF A LUNG CANCER CMllIlTEE.
R.Rami-Porta, G.Gonza'lez, L.Cirera,
J.A.De
A.Domingo, Lung
Marcos,
A.Salas,
Cancer
I.Mir,
Committee,
which
serves
in
G.Aguilar,
Hospital
umologists, primary
of
a population
January
1990
and
oncologists,
role
is to
IN SPAIN.
J.L.Heredia, R.Bastds, L.Esteban, P.Forcada, X.Tarroch, F.Tresserra,
(Barcelona), Spain. The Lung Cancer Committee
of
Mutua Hospital
170.000
consists all
de
M.Cuesta.
Terrassa,
Mutua
de
was
crea-
surgeons,
pne-
radiologists.
Its
of thoracic and
lung
Tarrassa
Terrassa,
inhabitants,
pathologists
register
C.Corbella,
cancer
cases
diagnosed
in the Hospital and to discuss diagnostic, staging and therapeutic options. From January 1990 to December 1992, 199 consecutive patients (183 males and 16 females) were registered. Histologic types were: squamous cell carcinoma (ca) 97 (4B%), edenoca 35 (17.5%), small cell ca 32 (16%), large cell ca 27 (13.5%). other B (4%). The most common clinical findings
were
chest
pain
69
(34%),
dyspnea 47 (23%). A lung nodule or x-ray finding: 118 (59%), followed (18%) and pneumonitis 36 (18%). in 130 (65%) cases. Therapy was
hemoptysis
57
(28%)
and
mass was the most common by hiliar enlargement 37
Bronchoscopy ss follows:
was diagnostic symptomatic on-
ly 85 (42.7%), chemotherapy 40 (20.2%), radiotherapy 12 (6%), laser 1 (O.S%), surgery 61 (30.6%). Type of surgery: complete resection 51 (B3.8%), incomplete resection 2 (3.3%), exploratory thoracotomy 5 (8%), diagnostic thoracotomy lung cell
Hecht, and J.O. Carlson. Departments of Pathology and Microbiology, Colorado State University, Fort Collins, CO. USA Ovine pulmonary carcinoma (OPC) is a naturally-occurring contagious bronchioloalveolar carcinoma of sheep that occurs widely throughout the world. The disease has been transmitted to newborn lambs using a cell-free tumor homogenate that contains particleassociated reverse transcriptase and a 25 kD protein that reacts with antiserum to Mason-Ptixer monkey virus and mouse mammary tumor virus. In an attempt to isolate the OPC-associated retrovirns and to elucidate its role in pulmonary oncogenesis, degenerate oligonucleotide primers were designed based on conserved regions of the capsid gene of these viruses. Retroviral RNA was isolated from an OPC-affected sheep from Peru and used as a substrate for reverse transcription. The PCR amplification product was cloned, sequenced and used as a probe in Southern hybridization of OPC tumor genomic DNA and tissue DNA from unaffected sheep. ln all sheep tested, the probe reacted with 15-25 restriction fragments for which a viral derivation was confirmed by further hybridization with pal and env probes derived from the related jaagsiekte sheep retrovirus, and by evidence of assortment in sheep families. lnitial experiments did not demonstrate any obvious differences in viral presence in OPC tumor DNA compared with DNA of unaffected lungs against the background of endogenous virus. The role of this type D retrovirus, if any, in the pathogenesis of OPC awaits further investigation.
198
197
ted
Analysis of a Type. D Retroviral Capsid Gene in a Contagious S.J. Bronchiohlveolar Carcinoma of Sheep. J.C. DeMartini,
3 (4.9%). cancer is ca is the
From this study, we can conclude still rare in females in Spain; predominant type; 3.- less that
that l.2.- squamous one third of
the patients can undergo surgical treatment, but with a high rate of complete resections; 4.- registration of cases by a Lung Cancer Committee allows to have a global picture of the disease within a certain population area.
LACKING GSThIl-ACTIVITY INDICATES INCREASED LUNG CANCER RISK IN INDIVIDUALS WITH ASBESTOS JIXI’OSUREAND LOW TOBACCO CONSUMPTION. G. Tomling, A.-K. Alexandrie, A. Rammg, P. R6mmw. Dept of
Thoracic Medicine, Karolinska Hospital, Stockholm and Dept of Toxicology, National Institute of Occupational Health, Sweden. The cytosolic enzyme GSTMl belongs to a family of glutathione transferases that detoxify certain activated forms of chemical carcinogens. Lack of activity is caused by the inherited absence of an intact GSTMl gene. The objectives of the present study was to evaluate a possible increased incidence of the genotypes associated with higher lung cancer risk among smokers and asbestos exposed individuals. 142 smokers, 81 with lung cancer and 61 with chronic obstructive pulmonary disease (COPD), were interviewed with regard to their occupational and smoking history. DNA was prepamd from froxen blood and PCR-based genotyping assays were used to determine GSTMl genotypes. N Lacking GSTM1-activity COPD 52,5X 61 81 56,896 Lung cancer, all Lung cancer, <25 pack years 14 78,6% Lung cancer, asbestos exposure 13 76,9% The proportion of patients lacking GSTMl-activity was approximately the same in the lung cancer group, the COPD-group, and in a group of 340 healthy volunteers. However, individuals lacking GSTMI-activity seem to get lung cancer after lower mbaccoexposure than individuals with enzyme activity. Further, the increased frequency of individuals lacking GSTMl-activity among the asbestos exposed lung cancer cases tight give some clues to asbestos carcinogenesis.
MANAGEMENT OF WNG CANCER IN A GENERAL HO!%ITAL ROLE OF A LUNG CANCER CMllIlTEE.
R.Rami-Porta, G.Gonza'lez, L.Cirera,
J.A.De
A.Domingo, Lung
Marcos,
A.Salas,
Cancer
I.Mir,
Committee,
which
serves
in
G.Aguilar,
Hospital
umologists, primary
of
a population
January
1990
and
oncologists,
role
is to
IN SPAIN.
J.L.Heredia, R.Bastds, L.Esteban, P.Forcada, X.Tarroch, F.Tresserra,
(Barcelona), Spain. The Lung Cancer Committee
of
Mutua Hospital
170.000
consists all
de
M.Cuesta.
Terrassa,
Mutua
de
was
crea-
surgeons,
pne-
radiologists.
Its
of thoracic and
lung
Tarrassa
Terrassa,
inhabitants,
pathologists
register
C.Corbella,
cancer
cases
diagnosed
in the Hospital and to discuss diagnostic, staging and therapeutic options. From January 1990 to December 1992, 199 consecutive patients (183 males and 16 females) were registered. Histologic types were: squamous cell carcinoma (ca) 97 (4B%), edenoca 35 (17.5%), small cell ca 32 (16%), large cell ca 27 (13.5%). other B (4%). The most common clinical findings
were
chest
pain
69
(34%),
dyspnea 47 (23%). A lung nodule or x-ray finding: 118 (59%), followed (18%) and pneumonitis 36 (18%). in 130 (65%) cases. Therapy was
hemoptysis
57
(28%)
and
mass was the most common by hiliar enlargement 37
Bronchoscopy ss follows:
was diagnostic symptomatic on-
ly 85 (42.7%), chemotherapy 40 (20.2%), radiotherapy 12 (6%), laser 1 (O.S%), surgery 61 (30.6%). Type of surgery: complete resection 51 (B3.8%), incomplete resection 2 (3.3%), exploratory thoracotomy 5 (8%), diagnostic thoracotomy lung cell
Hecht, and J.O. Carlson. Departments of Pathology and Microbiology, Colorado State University, Fort Collins, CO. USA Ovine pulmonary carcinoma (OPC) is a naturally-occurring contagious bronchioloalveolar carcinoma of sheep that occurs widely throughout the world. The disease has been transmitted to newborn lambs using a cell-free tumor homogenate that contains particleassociated reverse transcriptase and a 25 kD protein that reacts with antiserum to Mason-Ptixer monkey virus and mouse mammary tumor virus. In an attempt to isolate the OPC-associated retrovirns and to elucidate its role in pulmonary oncogenesis, degenerate oligonucleotide primers were designed based on conserved regions of the capsid gene of these viruses. Retroviral RNA was isolated from an OPC-affected sheep from Peru and used as a substrate for reverse transcription. The PCR amplification product was cloned, sequenced and used as a probe in Southern hybridization of OPC tumor genomic DNA and tissue DNA from unaffected sheep. ln all sheep tested, the probe reacted with 15-25 restriction fragments for which a viral derivation was confirmed by further hybridization with pal and env probes derived from the related jaagsiekte sheep retrovirus, and by evidence of assortment in sheep families. lnitial experiments did not demonstrate any obvious differences in viral presence in OPC tumor DNA compared with DNA of unaffected lungs against the background of endogenous virus. The role of this type D retrovirus, if any, in the pathogenesis of OPC awaits further investigation.
198
197
ted
Analysis of a Type. D Retroviral Capsid Gene in a Contagious S.J. Bronchiohlveolar Carcinoma of Sheep. J.C. DeMartini,
3 (4.9%). cancer is ca is the
From this study, we can conclude still rare in females in Spain; predominant type; 3.- less that
that l.2.- squamous one third of
the patients can undergo surgical treatment, but with a high rate of complete resections; 4.- registration of cases by a Lung Cancer Committee allows to have a global picture of the disease within a certain population area.
LACKING GSThIl-ACTIVITY INDICATES INCREASED LUNG CANCER RISK IN INDIVIDUALS WITH ASBESTOS JIXI’OSUREAND LOW TOBACCO CONSUMPTION. G. Tomling, A.-K. Alexandrie, A. Rammg, P. R6mmw. Dept of
Thoracic Medicine, Karolinska Hospital, Stockholm and Dept of Toxicology, National Institute of Occupational Health, Sweden. The cytosolic enzyme GSTMl belongs to a family of glutathione transferases that detoxify certain activated forms of chemical carcinogens. Lack of activity is caused by the inherited absence of an intact GSTMl gene. The objectives of the present study was to evaluate a possible increased incidence of the genotypes associated with higher lung cancer risk among smokers and asbestos exposed individuals. 142 smokers, 81 with lung cancer and 61 with chronic obstructive pulmonary disease (COPD), were interviewed with regard to their occupational and smoking history. DNA was prepamd from froxen blood and PCR-based genotyping assays were used to determine GSTMl genotypes. N Lacking GSTM1-activity COPD 52,5X 61 81 56,896 Lung cancer, all Lung cancer, <25 pack years 14 78,6% Lung cancer, asbestos exposure 13 76,9% The proportion of patients lacking GSTMl-activity was approximately the same in the lung cancer group, the COPD-group, and in a group of 340 healthy volunteers. However, individuals lacking GSTMI-activity seem to get lung cancer after lower mbaccoexposure than individuals with enzyme activity. Further, the increased frequency of individuals lacking GSTMl-activity among the asbestos exposed lung cancer cases tight give some clues to asbestos carcinogenesis.