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Marine toxins from the Pacific—IX. Some effects of ciguatoxin on isolated mammalian Atria
Toxlcoe, 1971, Vol. 9, pp . 437
14 . PerQamon Prass. Printed in Great Britain
ABSTRACTS FOR CARD INDEXES Aglycones of the toxins from the Cuvierian organs of Holothtvia forskdli and a new nomenclature for the aglycones from Holothurioideae : G>~txAxn HAS~~tI?l~ and GßRT VOLICWSII~i, Toxicon,1971, 9, 319. (Institute of Organic Chemistry, Technical University, Darmstadt, Germany). Abe6ract-From the G~vierian organs of Holothurta forakdlf three compounds have been isolated which are aglycones of the respective toxins . Their structures could be determined by means of spectroscopic methods. A new nomenclature for the aglycones of the toxins from holothurians is proposed . Isolation and partial purification of a toxin from Millepora alcicornis : LewxErrcE W. WrrrLB, ROB>;ttT E. MroDr.EaROOx and G~rAxr~s E. LANE, Toxicon, 1971, 9, 327. (Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, U.S .A .) . Abstract-A toxin from the Hydrozoan coral, Millepora alcicornls, has been partially purified by ammonium sulfate precipitation, anion~xchange chromatography, gel-filtration, and adsorption chromatography. The water soluble toxin is thermolabile, non~ialy7able, and pH sensitive. The r~6a for 20 g mice is 40 l+g protein per kg body wt. Isolation and purification of a toxin from Millepora dichotome : ROBERT E. M>DDLEBROOIC, LAWRENCE W. Wrrr>rE, EDWARD D. SCUBA and CFiARI.r?c E. LANE, Toxicon, 1971, 9, 333. (Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, U.S .A .) . Abstract-A water-soluble, non-dialy7able toxin has bcen isolated from the hydrozoan coral, Millepora dichotoma, collected at F.niwetok Atoll. The toxin was purified by anion exchange chromatography . The tn 6o for 20 g mice is 38 wg protein per kg body wt. The toxin is compared and contrasted with that isolated from the West Indian and Caribbean hydrozoan, Millepora aleicornis .
Marine toxins from the Pacifi~IX. Some effects of ciguatoxin on isolated mammalian Atria: H>DEYO OHSfnRA, Toxicon, 1971, 9, 337. (Hawaii Institute of Marine Biology, University of Hawaii, Honolulu, Hawaii, U.S .A .). Abstract--Ciguatoxin produced an initial negative inotropic and chronotropic effect which was followed by a positive inotropic and chronotropic effect on the isolated rat atria. The initial depression could be effectively blocked by atropine (1 x 10-' g per ml), and partially blocked by hexamethonium or hemicholinium-3 (2 x 10 -° gper ml). The positive inotropic and chronotropic action of ciguatoxin was reduced by pretreating the atria with MJ-1999 (1 x 10-° g per ml) or guauethidine (1 x 10-° g per ml). Abolition of the response to ciguatoxin could also be accomplished with asingle dose of 2mg perkg of reserpine givento the rat 24 hr before isolating the heart. It is not yet possible to correlate inotropic and chronotropic effects of the toxin with therelease ofcatecholamines, butit seems likely that excitatory effect of thetoxinmaybe elicited by a release of catecholamines from their stores. 437 TOXICON 1971 Yol. 9.
14 . PerQamon Prass. Printed in Great Britain
ABSTRACTS FOR CARD INDEXES Aglycones of the toxins from the Cuvierian organs of Holothtvia forskdli and a new nomenclature for the aglycones from Holothurioideae : G>~txAxn HAS~~tI?l~ and GßRT VOLICWSII~i, Toxicon,1971, 9, 319. (Institute of Organic Chemistry, Technical University, Darmstadt, Germany). Abe6ract-From the G~vierian organs of Holothurta forakdlf three compounds have been isolated which are aglycones of the respective toxins . Their structures could be determined by means of spectroscopic methods. A new nomenclature for the aglycones of the toxins from holothurians is proposed . Isolation and partial purification of a toxin from Millepora alcicornis : LewxErrcE W. WrrrLB, ROB>;ttT E. MroDr.EaROOx and G~rAxr~s E. LANE, Toxicon, 1971, 9, 327. (Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, U.S .A .) . Abstract-A toxin from the Hydrozoan coral, Millepora alcicornls, has been partially purified by ammonium sulfate precipitation, anion~xchange chromatography, gel-filtration, and adsorption chromatography. The water soluble toxin is thermolabile, non~ialy7able, and pH sensitive. The r~6a for 20 g mice is 40 l+g protein per kg body wt. Isolation and purification of a toxin from Millepora dichotome : ROBERT E. M>DDLEBROOIC, LAWRENCE W. Wrrr>rE, EDWARD D. SCUBA and CFiARI.r?c E. LANE, Toxicon, 1971, 9, 333. (Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, U.S .A .) . Abstract-A water-soluble, non-dialy7able toxin has bcen isolated from the hydrozoan coral, Millepora dichotoma, collected at F.niwetok Atoll. The toxin was purified by anion exchange chromatography . The tn 6o for 20 g mice is 38 wg protein per kg body wt. The toxin is compared and contrasted with that isolated from the West Indian and Caribbean hydrozoan, Millepora aleicornis .
Marine toxins from the Pacifi~IX. Some effects of ciguatoxin on isolated mammalian Atria: H>DEYO OHSfnRA, Toxicon, 1971, 9, 337. (Hawaii Institute of Marine Biology, University of Hawaii, Honolulu, Hawaii, U.S .A .). Abstract--Ciguatoxin produced an initial negative inotropic and chronotropic effect which was followed by a positive inotropic and chronotropic effect on the isolated rat atria. The initial depression could be effectively blocked by atropine (1 x 10-' g per ml), and partially blocked by hexamethonium or hemicholinium-3 (2 x 10 -° gper ml). The positive inotropic and chronotropic action of ciguatoxin was reduced by pretreating the atria with MJ-1999 (1 x 10-° g per ml) or guauethidine (1 x 10-° g per ml). Abolition of the response to ciguatoxin could also be accomplished with asingle dose of 2mg perkg of reserpine givento the rat 24 hr before isolating the heart. It is not yet possible to correlate inotropic and chronotropic effects of the toxin with therelease ofcatecholamines, butit seems likely that excitatory effect of thetoxinmaybe elicited by a release of catecholamines from their stores. 437 TOXICON 1971 Yol. 9.