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Mast cell stabilising activity of Bacopa monnieri
Fitoterapia 72 Ž2001. 284᎐285
Short report
Mast cell stabilising activity of Bacopa monnieri D.S. Samiulla, D. PrashanthU , A. Amit Bioassay Unit, Research & De¨ elopment Centre, Natural Remedies P¨ t. Ltd., Plot No. 5B, Veerasandra Indl. Area, Hosur Road, Bangalore 561 229, India Received 17 July 2000; accepted in revised form 5 October 2000
Abstract Successive petroleum ether, chloroform, methanol and water extracts of Bacopa monnieri were tested Žin vitro. for mast cell stabilising effect. The methanolic fraction exhibited potent activity comparable to disodium cromoglycate, a known mast cell stabiliser. 䊚 2001 Elsevier Science B.V. All rights reserved. Keywords: Bacopa monnieri; Mast cell stabilisation
Plant. Bacopa monnieri L. ŽScrophulariaceae. leaves were collected from Calcutta, in February 2000. The sample was authenticated by our Pharmacognosy department where the voucher specimen ŽBAUrBACr02. is deposited. Use in traditional medicine. As a laxative, intellect promoting, in bronchitis and several skin diseases w1x. Previously isolated constituents. Alkaloids, saponins w2x. Tested material. Petrol Ž60᎐80⬚C., CHCl 3 , MeOH and water successive extracts Žyields: 1.75, 5, 12, 17%, respectively.. Preliminary phytochemical screening w3,4x gave positive tests for sterols Žpetrol extract ., alkaloids ŽCHCl 3 extract., saponins, alkaloids ŽMeOH extract. and tannins Žwater extract.. U
Corresponding author. E-mail address: [email protected] ŽD. Prashanth .. 0367-326Xr01r$ - see front matter 䊚 2001 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 0 0 . 0 0 3 0 9 - 9
D.S. Samiulla et al. r Fitoterapia 72 (2001) 284᎐285
285
Studied activity. Mast cell stabilisation assay Žin vitro. w5x. Disodium cromoglycate was used as the reference standard w6,7x. Compound 48r80 was used as mast cell degranulator w8x. Results. Reported in Table 1. Table 1 Mast cell stabilisation activity of Bacopa monnieri leaf successive extracts a Tested material Žconcentration .
Degranulation Ž%.
Control Compound 48r80 Ž4 grml. Petrol extract Ž10 grml. Chloroform extract Ž10 grml. Methanol extract Ž10 grml. Water extract Ž10 grml. Disodium cromoglycate Ž10 grml.
29.7 " 3.7UUU 85.8 " 1.8 72.7 " 3.1U 80.0 " 2.8 38.0 " 4.2UUU 75.3 " 1.6UU 41.7 " 3.4UUU
a Values are mean " S.E.M. Ž n s 6.; Žnegative control., Student’s t-test.
U
P - 0.05,
UU
P - 0.005,
UUU
P - 0.001 vs. compound 48r80
Conclusions. The methanolic extract exhibited a potent mast cell stabilisation effect comparable to disodium cromoglycate. This result indicates the potential usefulness of Bacopa monnieri leaves in allergic conditions.
Acknowledgements The authors are thankful to Mr R.K. Agarwal, Chairman, Mrs. Natural Remedies Pvt. Ltd., Bangalore for his encouragement and Mrs Geetha T.S. for secretarial assistance.
References w1x Nadkarni KM. Indian materia medica, 1. Bombay: Popular Prakashan, 1993:624. w2x Sathyavati GV, Raina MK, Sharma M. Medicinal plants of India, 1. New Delhi: Indian Council for Medicinal Research, 1976:118. w3x Trease EG, Evans WC. Text book of pharmacognosy. 13th ed. London: Bailliere Tindall, 1989:546. w4x Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 4th ed. Pune: Nirali Prakashan, 1996:123᎐124. w5x Rajasekaran M, Sudhakaran C, Pradhan SC, Bapna JS, Nair AGR. J Drug Res 1989;2:179. w6x Orr TSC, Hall DE, Gwilliam JM, Cox JSC. Life Sci 1971;10:805. w7x Marshall R. Thorax 1972;27:38. w8x Kanemoto T, Kasugai T, Yamatodani A, Ushio H, Mochizuki Tohya K, Kimura M, Nishimura M, Kitamura Y. Int Arch Allergy Immunol 1993;100:99.
Short report
Mast cell stabilising activity of Bacopa monnieri D.S. Samiulla, D. PrashanthU , A. Amit Bioassay Unit, Research & De¨ elopment Centre, Natural Remedies P¨ t. Ltd., Plot No. 5B, Veerasandra Indl. Area, Hosur Road, Bangalore 561 229, India Received 17 July 2000; accepted in revised form 5 October 2000
Abstract Successive petroleum ether, chloroform, methanol and water extracts of Bacopa monnieri were tested Žin vitro. for mast cell stabilising effect. The methanolic fraction exhibited potent activity comparable to disodium cromoglycate, a known mast cell stabiliser. 䊚 2001 Elsevier Science B.V. All rights reserved. Keywords: Bacopa monnieri; Mast cell stabilisation
Plant. Bacopa monnieri L. ŽScrophulariaceae. leaves were collected from Calcutta, in February 2000. The sample was authenticated by our Pharmacognosy department where the voucher specimen ŽBAUrBACr02. is deposited. Use in traditional medicine. As a laxative, intellect promoting, in bronchitis and several skin diseases w1x. Previously isolated constituents. Alkaloids, saponins w2x. Tested material. Petrol Ž60᎐80⬚C., CHCl 3 , MeOH and water successive extracts Žyields: 1.75, 5, 12, 17%, respectively.. Preliminary phytochemical screening w3,4x gave positive tests for sterols Žpetrol extract ., alkaloids ŽCHCl 3 extract., saponins, alkaloids ŽMeOH extract. and tannins Žwater extract.. U
Corresponding author. E-mail address: [email protected] ŽD. Prashanth .. 0367-326Xr01r$ - see front matter 䊚 2001 Elsevier Science B.V. All rights reserved. PII: S 0 3 6 7 - 3 2 6 X Ž 0 0 . 0 0 3 0 9 - 9
D.S. Samiulla et al. r Fitoterapia 72 (2001) 284᎐285
285
Studied activity. Mast cell stabilisation assay Žin vitro. w5x. Disodium cromoglycate was used as the reference standard w6,7x. Compound 48r80 was used as mast cell degranulator w8x. Results. Reported in Table 1. Table 1 Mast cell stabilisation activity of Bacopa monnieri leaf successive extracts a Tested material Žconcentration .
Degranulation Ž%.
Control Compound 48r80 Ž4 grml. Petrol extract Ž10 grml. Chloroform extract Ž10 grml. Methanol extract Ž10 grml. Water extract Ž10 grml. Disodium cromoglycate Ž10 grml.
29.7 " 3.7UUU 85.8 " 1.8 72.7 " 3.1U 80.0 " 2.8 38.0 " 4.2UUU 75.3 " 1.6UU 41.7 " 3.4UUU
a Values are mean " S.E.M. Ž n s 6.; Žnegative control., Student’s t-test.
U
P - 0.05,
UU
P - 0.005,
UUU
P - 0.001 vs. compound 48r80
Conclusions. The methanolic extract exhibited a potent mast cell stabilisation effect comparable to disodium cromoglycate. This result indicates the potential usefulness of Bacopa monnieri leaves in allergic conditions.
Acknowledgements The authors are thankful to Mr R.K. Agarwal, Chairman, Mrs. Natural Remedies Pvt. Ltd., Bangalore for his encouragement and Mrs Geetha T.S. for secretarial assistance.
References w1x Nadkarni KM. Indian materia medica, 1. Bombay: Popular Prakashan, 1993:624. w2x Sathyavati GV, Raina MK, Sharma M. Medicinal plants of India, 1. New Delhi: Indian Council for Medicinal Research, 1976:118. w3x Trease EG, Evans WC. Text book of pharmacognosy. 13th ed. London: Bailliere Tindall, 1989:546. w4x Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 4th ed. Pune: Nirali Prakashan, 1996:123᎐124. w5x Rajasekaran M, Sudhakaran C, Pradhan SC, Bapna JS, Nair AGR. J Drug Res 1989;2:179. w6x Orr TSC, Hall DE, Gwilliam JM, Cox JSC. Life Sci 1971;10:805. w7x Marshall R. Thorax 1972;27:38. w8x Kanemoto T, Kasugai T, Yamatodani A, Ushio H, Mochizuki Tohya K, Kimura M, Nishimura M, Kitamura Y. Int Arch Allergy Immunol 1993;100:99.