Measurement of platelet adherence to collagen

THROMBOSIS &Pergmnon

RESEARCH 14; 519-520 Printed Press Ltd.1979.

LETTER

in Great Britain Ou49-38fi8/79/03@~ -0519

#02.00/O

TO THE EDITORS-IN-CHIEF

I4lzwmm4ENToF

--To-?

MidumlJ;Mmt Dapmhentof&dicina,UniversityofAlberta, Auwta,Czmada. am, (Received

Accepted

15.12.1978.

Axmjor critioismofmny~ collam

by A.L.

Copley)

formaauringphte~tadherenoe

(PAC)isthattkirreSUltSlrraybeinvalidatedbyCan

let aggmgatim ingPACMi&uaea

(1,2). Ihave reazntly m EDAt

inhibit

to

ourmltplata-

a techiqm

forquantitat-

plateletaggregaticm (3). Inny

initial

st&iesaggxega~wasexolu%dby#useaxkrastndmosmpyandbyobsarvatioslsthataddi~ofadenoainedi~~,~~,ahd~~qen, didIlot~aggregationintheEMa-plateletrichplaslnausedintbeprooedure. Imvwiahtorepo~aMiticnalevihnozthatplateletaggmgaticm &x?snotoa%rinthaEthoddescribed. Preino&ationofplateletswithprostaglandinEl oflpg/ml

andlOug/ml

(pGE1)ataxmntrations

for 30 and for 60 minutes hadnoeffectonPP13.

pretiuslynqxxtedbyoUeratheae

As

cmomtratimaofPQ$mmkedlyinhibited

~llaganindumdpla~etaggregationinoitratedplaanm.

Platehtadtmmoe

tocollagenhasalebbeen~~wiMthetechniquein~individualwith lhmlhsthenia.Inthispati.entPAcwasnonnal.mthisdiso*platelet~ agqegationtooolla+nisdefeotive. *maa

ummntofPACitwxldbeantioipat&thatPACin

hlhpresencJe nomu.

If platelet aggnzgation occum during mtkniaand

ofP&l~~dbe~~ti~ys~~rather~being

Findlly,post-adhenenoe~ofw~a~andplatelets~~been

maminedbyt?mamissionel~mi~

Very fewoftheplatelets

pre8entwer@inaxlmotwitheaohother.

Allofthesawerecloaelyaasoci519

PLATELET ADHERENCE TO COLLAGEN

520

atedwithadlagen

v01.1+,N0.2/3

fibrils,definiteaggregalzswerenotcbsemed,andthe

3zmwngplatele~remainedisolated. (3)make it e !&esestLdies,tog&herwithdmsep reviouslye ~lyunlikely~tplateleta~~~interferes~.thquentitatiarof PAcinth@p?XXZdUrfSdWdhd.

1. SIX& J.J.,V, 1977.

J. 'Ihelmmstatic plug. SeminHenvatol.14, 265,

2. -, H.R., TSCHXP, T.B.,WEISS,H.J. Plateletinteracticxlwith collagmfibrilsinflmingblood. II. In@xedadhesi~~~aggregati~ inbleedingdisorders. AaxqarkmwithsdxdMhSum. --. 37, 17, 1977. 3. MWF, M.J. Plateletadhemme toauagzll: as&de, +mJmKbRS. quantitative~thodforitsmasuremnt.

qmddble, ll, 729, 1977.