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Mechanisms of functional recovery of spinal cord injury after primates using dental pulp stem cells
Poster Session can be diagnosed at the earliest time. Crush injury and partial nerve amputation can be diagnosed after a minimum of 10 weeks of observation. This study proves that SCV is an effective factor in the evaluation of nerve injury and regeneration.
POSTER 122 Mechanisms of functional recovery of spinal cord injury after primates using dental pulp stem cells F. Kano: Nagoya University Graduate school of medicine, A. Yamamoto, T. Mita, M. Ueda Spinal cord injury are assumed difficult to recover due to various factors. In recent years, the study of cell transplantation therapy for spinal cord injury have been noted. Previously, we showed that the recovery of motor function improved for lower limb of rats using human dental pulp stem cells. However, compared with the binding mode of corticospinal tract nerve in primates, in rats it is very different. To develop the new treatment after spinal cord injury in humans, we have compared the effect healing of human dental pulp stem cells against to spinal cord injury using a rhesus monkey with the characteristics behavioral physiology, anatomy the same to humans. This study was performed as a process for functional recovery of dexterous finger movement that developed particularly in primates. We performed a hemi-section on the left side of the C5 spinal cord of rhesus monkeys that thoroughly trained finger movement function evaluation. Immediately, we transplanted stem cells from human exfoliated deciduous teeth (SHED) 1*10^6 cells at Hamilton syringe each sites leaion area and its rostral sites and caudal sites. The control group was injected with PBS I at the same site for comparison. Check the hemiplegia of monkey day after surgery, we started the rehabilitation of limb then.
POSTER 123 Functional recovery of Alzheimer’s disease using Stem Cells from Human Exfoliated Deciduous Tooth-Derived Conditioned Medium T. Mita: Nagoya University Graduate school of medicine, A. Yamamoto, F. Kano, H. Hattori, M. Ueda Introduction: Alzheimer’s disease (AD), a progressive neurodegenerative disease, is characterized by deterioration of cognitive function accompanying with deposition of b-amyloid (Ab) peptides. Ab peptides are liberated from larger transmembrane amyloid precursor proteins AAOMS 2014
(APP) and generate Ab42/Ab40 peptides. These aberrant Ab peptides form amyloid plaques induce M1-type pro-inflammatory microglia, releasing high levels of cytokines, glutamate, reactive oxygen species, and nitric oxide (NO). This oxidative stress accelerates generation of 3-nitrotyrosine in neuron and consequently suppresses the catecholamine synthesis and depresses neuro-synaptic transmission. Inhibition of the neurotoxic factor production may be a novel AD treatment. We have reported strong immunosuppressive activities of serum free conditioned medium derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED-CM). Purpose: In this study, we examined the therapeutic benefits of SHED-CM, Bone marrow mesenchymal stem cells (BMMSC)-CM and skin fibroblast (Fibro)-CM for treatment of the Ab peptide injected mouse ADlike model. Materials and Methods: Mouse AD-like model was generated by the i.c.v. injection of oligomerized 5mg of Ab1-40, or Ab40–1 was into the 9-week-old ICR mice brain. Twenty-four hours after the i.c.v. injection, a total of 50ml of various type CM, were administered to each mice via the olfactory pathway. The therapeutic effects were evaluated by the behavioral, biochemical and histological examination. All experiments were performed in accordance with the Guidelines for Animal Experiments of Nagoya University Graduate School of Medicine. Result: SHED-CM suppressed the expressions of proinflammatory cytokines, iNOS and 3-nitrotyrosine and improved cognitive function of the mouse AD-like model. Conclusion: SHED-CM treatment may provide a significant therapeutic benefits for treating cognitive dysfunction of AD.
POSTER 124 Comparison of Nerve Repair Surgery for Inferior Alveolar Nerve M. Takata: Tokyo Dental College, M. Murayama, Y. Fujimoto, K. Sasaki, T. Shibahara Introduction: The most common form of inferior alveolar nerve (IAN) damage in dentistry is partial nerve amputation. Surgical repair is necessary for this condition and when it is left untreated for long periods, neural scarring can cause symptoms of allodynia. Several approaches with microneurosurgery and regeneration materials exist for nerve repair however no definite treatment method has been established. In order to identify the best neurosurgical treatment for partial nerve amputation, damages were applied to the IAN of rabbits and histopathological observations were investigated and compared. Material and Method: Partial nerve amputation injuries were applied to the IAN of rabbits then nerve repair surgery were performed. Nerve repair surgery established 3 groups (z@non coating group ,zAPGA coating e-121
POSTER 122 Mechanisms of functional recovery of spinal cord injury after primates using dental pulp stem cells F. Kano: Nagoya University Graduate school of medicine, A. Yamamoto, T. Mita, M. Ueda Spinal cord injury are assumed difficult to recover due to various factors. In recent years, the study of cell transplantation therapy for spinal cord injury have been noted. Previously, we showed that the recovery of motor function improved for lower limb of rats using human dental pulp stem cells. However, compared with the binding mode of corticospinal tract nerve in primates, in rats it is very different. To develop the new treatment after spinal cord injury in humans, we have compared the effect healing of human dental pulp stem cells against to spinal cord injury using a rhesus monkey with the characteristics behavioral physiology, anatomy the same to humans. This study was performed as a process for functional recovery of dexterous finger movement that developed particularly in primates. We performed a hemi-section on the left side of the C5 spinal cord of rhesus monkeys that thoroughly trained finger movement function evaluation. Immediately, we transplanted stem cells from human exfoliated deciduous teeth (SHED) 1*10^6 cells at Hamilton syringe each sites leaion area and its rostral sites and caudal sites. The control group was injected with PBS I at the same site for comparison. Check the hemiplegia of monkey day after surgery, we started the rehabilitation of limb then.
POSTER 123 Functional recovery of Alzheimer’s disease using Stem Cells from Human Exfoliated Deciduous Tooth-Derived Conditioned Medium T. Mita: Nagoya University Graduate school of medicine, A. Yamamoto, F. Kano, H. Hattori, M. Ueda Introduction: Alzheimer’s disease (AD), a progressive neurodegenerative disease, is characterized by deterioration of cognitive function accompanying with deposition of b-amyloid (Ab) peptides. Ab peptides are liberated from larger transmembrane amyloid precursor proteins AAOMS 2014
(APP) and generate Ab42/Ab40 peptides. These aberrant Ab peptides form amyloid plaques induce M1-type pro-inflammatory microglia, releasing high levels of cytokines, glutamate, reactive oxygen species, and nitric oxide (NO). This oxidative stress accelerates generation of 3-nitrotyrosine in neuron and consequently suppresses the catecholamine synthesis and depresses neuro-synaptic transmission. Inhibition of the neurotoxic factor production may be a novel AD treatment. We have reported strong immunosuppressive activities of serum free conditioned medium derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED-CM). Purpose: In this study, we examined the therapeutic benefits of SHED-CM, Bone marrow mesenchymal stem cells (BMMSC)-CM and skin fibroblast (Fibro)-CM for treatment of the Ab peptide injected mouse ADlike model. Materials and Methods: Mouse AD-like model was generated by the i.c.v. injection of oligomerized 5mg of Ab1-40, or Ab40–1 was into the 9-week-old ICR mice brain. Twenty-four hours after the i.c.v. injection, a total of 50ml of various type CM, were administered to each mice via the olfactory pathway. The therapeutic effects were evaluated by the behavioral, biochemical and histological examination. All experiments were performed in accordance with the Guidelines for Animal Experiments of Nagoya University Graduate School of Medicine. Result: SHED-CM suppressed the expressions of proinflammatory cytokines, iNOS and 3-nitrotyrosine and improved cognitive function of the mouse AD-like model. Conclusion: SHED-CM treatment may provide a significant therapeutic benefits for treating cognitive dysfunction of AD.
POSTER 124 Comparison of Nerve Repair Surgery for Inferior Alveolar Nerve M. Takata: Tokyo Dental College, M. Murayama, Y. Fujimoto, K. Sasaki, T. Shibahara Introduction: The most common form of inferior alveolar nerve (IAN) damage in dentistry is partial nerve amputation. Surgical repair is necessary for this condition and when it is left untreated for long periods, neural scarring can cause symptoms of allodynia. Several approaches with microneurosurgery and regeneration materials exist for nerve repair however no definite treatment method has been established. In order to identify the best neurosurgical treatment for partial nerve amputation, damages were applied to the IAN of rabbits and histopathological observations were investigated and compared. Material and Method: Partial nerve amputation injuries were applied to the IAN of rabbits then nerve repair surgery were performed. Nerve repair surgery established 3 groups (z@non coating group ,zAPGA coating e-121