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Mechanisms of viral impact on asthma exacerbations
S264 Abstracts
J ALLERGY CLIN IMMUNOL FEBRUARY 2004
952
Mechanisms of Viral Impact on Asthma Exacerbations
TUESDAY
M. R. Khaitov1, D. U. Trofimov1, T. V. Petrova1, K. P. Yakovleva1, M. N. Boldyreva1, M. N. Yartsev1, N. I. Ilina1, L. M. DuBuske2, L. P. Alexeev1; 1Institute of Immunology, Moscow, RUSSIAN FEDERATION, 2Immunology Research Institute of New England, Fitchburg, MA. RATIONALE: Respiratory viruses are a frequent cause of wheezing in infants and children. 60 - 85% of asthma exacerbations in children and up to 50% of asthma exacerbations in adults are attributed to respiratory viruses. Previous studies investigated the role of rhinoviruses, RS-viruses and adenoviruses in development of asthma exacerbations in children hospitalized in the clinic of the Institute of Immunology (Moscow, Russia). Rhinovirus was the most frequently detected – 33 samples (24.8% of all tested samples), adenovirus – 5 samples (3.8%), RSV– 4 samples (3.0%), and mixed infections (rhinovirus and RSV) – 1 sample (0.7%). Among positive samples rhinovirus accounted for 76.7%, adenovirus - 11.6%, RSV - 9.3%, and mixed infection - 2.3%. METHODS: Primers for PCR diagnostics of Parainfluenza viruses of 1, 2 and 3 type and coronaviruses OC43 and 229E were developed. Investigation of HLA-alleles and genotypes was conducted in patients with virus-induced bronchial asthma to reveal HLA-associated markers of susceptibility to viral-induced asthma. RESULTS: An increased frequency of HLA-DRB1*13 specificity and reduced frequencies of HLA-DRB1*04 and HLA-DRB1*11 specificities were noted in the viral-induced asthmatics compared to the healthy control group. A minimal increase of HLA-DRB1 homozygotes - 21% was also noted in comparison with the control group - 14%. CONCLUSIONS: Investigation of HLA-haplotype in patients with viralinduced outbreaks of asthma via HLA-genotyping may reveal more evi-
J ALLERGY CLIN IMMUNOL VOLUME 113, NUMBER 2
Abstracts S265
TUESDAY
dent associations between HLA-haplotypes and individual respiratory viral infections. Funding: Institute of Immunology
J ALLERGY CLIN IMMUNOL FEBRUARY 2004
952
Mechanisms of Viral Impact on Asthma Exacerbations
TUESDAY
M. R. Khaitov1, D. U. Trofimov1, T. V. Petrova1, K. P. Yakovleva1, M. N. Boldyreva1, M. N. Yartsev1, N. I. Ilina1, L. M. DuBuske2, L. P. Alexeev1; 1Institute of Immunology, Moscow, RUSSIAN FEDERATION, 2Immunology Research Institute of New England, Fitchburg, MA. RATIONALE: Respiratory viruses are a frequent cause of wheezing in infants and children. 60 - 85% of asthma exacerbations in children and up to 50% of asthma exacerbations in adults are attributed to respiratory viruses. Previous studies investigated the role of rhinoviruses, RS-viruses and adenoviruses in development of asthma exacerbations in children hospitalized in the clinic of the Institute of Immunology (Moscow, Russia). Rhinovirus was the most frequently detected – 33 samples (24.8% of all tested samples), adenovirus – 5 samples (3.8%), RSV– 4 samples (3.0%), and mixed infections (rhinovirus and RSV) – 1 sample (0.7%). Among positive samples rhinovirus accounted for 76.7%, adenovirus - 11.6%, RSV - 9.3%, and mixed infection - 2.3%. METHODS: Primers for PCR diagnostics of Parainfluenza viruses of 1, 2 and 3 type and coronaviruses OC43 and 229E were developed. Investigation of HLA-alleles and genotypes was conducted in patients with virus-induced bronchial asthma to reveal HLA-associated markers of susceptibility to viral-induced asthma. RESULTS: An increased frequency of HLA-DRB1*13 specificity and reduced frequencies of HLA-DRB1*04 and HLA-DRB1*11 specificities were noted in the viral-induced asthmatics compared to the healthy control group. A minimal increase of HLA-DRB1 homozygotes - 21% was also noted in comparison with the control group - 14%. CONCLUSIONS: Investigation of HLA-haplotype in patients with viralinduced outbreaks of asthma via HLA-genotyping may reveal more evi-
J ALLERGY CLIN IMMUNOL VOLUME 113, NUMBER 2
Abstracts S265
TUESDAY
dent associations between HLA-haplotypes and individual respiratory viral infections. Funding: Institute of Immunology