Membrane proteins

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--- : A&d&z& P~ute&i., A.- @zi,‘.~. -Brodbeck and PZ%hler (Editors). Springer-Verlag, .- Berlin, Heidelberg,, Neti York, -1981ixii + 256 pp., DM49, $22.30. _ : In the-days when science &as conducted at a gentlemanly pace,-scientists used .to make wisecracks about colleagues &ho: published three quick (original) articles and ‘then immediately follow& that- with .a review -based on them. The techniques of _multiplying-‘publicationshave been tith.us so long now that-we no longer react .to new publications with the-qutitionswhy? and furwhom?. However, as a reviewer, I imn@iatcly reacte& to .yet another book oh membrane proteins. .The an&em. to my ~questionsare actually q&reassuring in this case. Let me start by saying that this book is a laboratory .m&nutl based on the International Advanced Course on Membrane Proteins organized by -FEBS and the Swiss Commission on Molecular Biology in Berne, 1980. The course dealt primarily with the techniques for h~&dling membrane-bound enzymes and that is the emphasis of the book. This -is obvious1y.a strength for. those wishing to learn about the isolation of specific proteins (e.g. phosphohpase A,, acetylcholine receptor, acetylcholine e&erase; cytochiome c oxidase,.etc.), but the individual protocols may not be very useful for other proteins. The saxtiemay be said of the experiments on reconstitution, w&S include the jt&ylcholine receptor, mitdchondrial ADP/_ATP carrier protein, purple’membrane, ubiquinone reductase and redcell membrane band 3. The section on analytical techniques (e.g. electrophoresis, immunoelectrophoresis, thin-layer chromatography, etc.),~~‘moregenerally applicable and potentially more useful. This isalso true of .the prottilson membrane modification methods (hydrophobic and hydrophilic labeling; cros+king laheling);.spectral. techniques (spin labels; fluorescencespectioscopy, potentiomeij &ith’dyes, circular -dichroism) .and other .physical methods (monoiayer &u&s, capacitance -measurement,eiectron microscopy). The variety~‘ofm&hods listedindi&zs. that. the.editors have cove&l a very wide range of: ihk. techniques being used today -‘in~re&Grch~ on ~membratieproteins. The de&iption~_m ~each~e%pe$ment -appears adequatej.:aud the. list] of references -at the end of each chapter provides leads’ for those interested infurther study. _.:. .

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