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Mercury toxicokinetics during larval and juvenile stages in rainbow trout
S288
Abstracts / Toxicology Letters 196S (2010) S37–S351
P304-006 Occurrence of perfluoroalkyl substances in Svitava and Svratka rivers, Czech Republic J. Kovarova 1 , P. Marsalek 1 , J. Jurcikova 2 , Z. Svobodova 1 1
University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic, 2 Institute of Public Health Ostrava, Czech Republic Perfluoroalkyl substances (PFAS, PFCs) represent a group of man-made chemicals constituting persistent or accumulative compounds of considerable negative effects to live organisms or human health. Content of PFAS in fish all over the world is well-known and the consumption of contaminated fish causes an increase in PFC body burdens. In this study seven perfluorinated compounds in fish serum from seven sites of Svitava and Svratka rivers in Brno conurbation, Czech Republic were determined. Content of perfluorohexane sulphonate (PFHxS), perfluorooctane sulphonate (PFOS), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), Nmethyl perfluorooctane sulphonamide (N-EtFOSA), fluorooctane sulphonamide (FOSA) and perfluoro-octenoic acid (FHUEA) were analyzed by high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) in serum of chub (Leuciscus cephalus L.). Major PFAS detected was PFOS (38.86–57.78 ng/mL with the mean of 48.659 ± 11.869 ng/mL), whereas other six perfluoroalkyls’ concentrations ranged between 0.240 and 4.040 ng/mL in all sampling sites. The most remarkable PFAS contamination was recorded on locality Bílovice—below waste water treatment plant (WWTP), where content of PFOS were also the highest. The lowest PFAS concentrations were measured on locality Rajhradice (PFHxS, FOSA, N-metFOSA, FHUEA), Modøice (PFOS) and Knínièky (PFOA, PFNA). It could be concluded that urban and suburban areas are exposed to increased levels of PFAS and that discharge of WWTP present confident risk factor for local PFAS storage in surface waters. doi:10.1016/j.toxlet.2010.03.907
P304-007 Mercury toxicokinetics during larval and juvenile stages in rainbow trout K. Kruzikova, R. Kensova, J. Havranek, Z. Svobodova University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic The aim of our study was to assess mercury content in tissues of rainbow trout (Oncorhychus mykiss) during the development stages in dependence on mercury content in fish feed. The measurement was conducted in feed, muscle, liver, kidney and in the fish homogenates every two months during one cycle of fish stock breeding. Atomic absorption spectrometry (analyzer AMA 254) was used for the determination. The total of 75 fish was used in the study. The mercury content increased during the breeding, the lowest content was detected in the whole body homogenate at the beginning of the experiment (4 ± 2 g/kg), whereas the highest mercury concentration was found in the muscle at the end of the experiment (128 ± 43 g/kg). The content of mercury in the liver and kidney did not differ significantly from the content in the muscle and ranged from 20 to 163 g/kg. Our results show a positive correlation between mercury level in fish and weight/length/age of fish. Three different feeds (A, B, C) were used in sequence during the rearing of rainbow trout. The levels of mercury were as follows: A = 12.6 gHg/kg,
B = 40.1 g/kg, C = 85.9 g/kg. Feeds A and C were used for one month at the beginning (A) and one month at the end (C) of the monitored period. Thus, for most of the experiment (16 months) the fish were supplied with feed B. Although the input of mercury (in feed) was practically the same during the 18 months, the mercury level increased in the muscle, liver and kidney of the fish. Acknowledgements: The study was supported by the project MSM 6215712402 “Veterinary aspect of food safety and quality”. doi:10.1016/j.toxlet.2010.03.908
P304-008 Aluminum analyses in Montrichardia linifera (Aninga), a wide range distributed Amazon macrophyte N. Santos Serrão De Castro 1 , A.L. Ferreira De Almeida Lins 2 , E. Conceic¸ão De Oliveira Santos 1 , I. Maura De Jesus 1 , C. Nahum Alves 3 , M. De Oliveira Lima 1 Evandro Chagas Institute, Brazil, 2 Museu Paraense Emilio Goeldi, Brazil, 3 Federal University of Pará, Brazil
1
Introduction: It is well documented that the Amazonia has a rich biodiversity and good mineral resources. In recent years, many mineral industries were attracted to the Amazonia, particularly to the city of Barcarena, Pará State, where an industrial complex of the aluminum production chain was installed. Objectives: To evaluate the Al concentration in root, shoot and leaf samples of Montrichardia linifera in four different rivers near industrial complex. Was quantified Al in sediments and physiochemical waters for environmental characterization. Methodology: The samples of M. linifera were collected, tissues were separated, washed with deionizer water, dried at 40 ± 5 ◦ C and homogenized. An amount of 200 mg for each sample were submitted to acid digestion (HNO3 + HClO4 ) and heated for 48 h (80 ± 5 ◦ C). The sediments were dried, particle separation (<270 mesh) and digested (HNO3 + HClO4 + HF), heated for 4 h (95 ± 5 ◦ C), centrifuged and supernatant solution was removed. Water physicochemical was performed by multiparameter probe and spectrophotometer analyses. Al analyses were performed through ICP-OES. GBW07604 and IAEA-SL-1 were used for quality control. Results: M. linifera collected in rivers near the industrial complex (Dendê, Curuperê and Murucupi) presented high concentrations of Al in roots and shoots, compared with the samples collected in Arapiranga (control). The analysis of Al sediments also indicates its high concentration in the rivers Dendê, Curuperê and Murucupi. Arapiranga presents the lowest concentrations of Al in sediments. Multivariate analysis demonstrated a positive correlation between sediments and shoots. Conclusions: M. linifera could survive at high Al concentration environments. Additionally, Al in roots and shoots reproduce clearly the profile of this metal in ambient. The physiochemical analysis supported the characterization of the outline of the rivers. This study indicates that industrial effluents are enhancing the naturally occurring Al concentration in sediments. Analysis in M. linifera reveals that the Al resulting from impacted areas is entering in ecosystem. doi:10.1016/j.toxlet.2010.03.909
Abstracts / Toxicology Letters 196S (2010) S37–S351
P304-006 Occurrence of perfluoroalkyl substances in Svitava and Svratka rivers, Czech Republic J. Kovarova 1 , P. Marsalek 1 , J. Jurcikova 2 , Z. Svobodova 1 1
University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic, 2 Institute of Public Health Ostrava, Czech Republic Perfluoroalkyl substances (PFAS, PFCs) represent a group of man-made chemicals constituting persistent or accumulative compounds of considerable negative effects to live organisms or human health. Content of PFAS in fish all over the world is well-known and the consumption of contaminated fish causes an increase in PFC body burdens. In this study seven perfluorinated compounds in fish serum from seven sites of Svitava and Svratka rivers in Brno conurbation, Czech Republic were determined. Content of perfluorohexane sulphonate (PFHxS), perfluorooctane sulphonate (PFOS), perfluorooctanoate (PFOA), perfluorononanoate (PFNA), Nmethyl perfluorooctane sulphonamide (N-EtFOSA), fluorooctane sulphonamide (FOSA) and perfluoro-octenoic acid (FHUEA) were analyzed by high performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) in serum of chub (Leuciscus cephalus L.). Major PFAS detected was PFOS (38.86–57.78 ng/mL with the mean of 48.659 ± 11.869 ng/mL), whereas other six perfluoroalkyls’ concentrations ranged between 0.240 and 4.040 ng/mL in all sampling sites. The most remarkable PFAS contamination was recorded on locality Bílovice—below waste water treatment plant (WWTP), where content of PFOS were also the highest. The lowest PFAS concentrations were measured on locality Rajhradice (PFHxS, FOSA, N-metFOSA, FHUEA), Modøice (PFOS) and Knínièky (PFOA, PFNA). It could be concluded that urban and suburban areas are exposed to increased levels of PFAS and that discharge of WWTP present confident risk factor for local PFAS storage in surface waters. doi:10.1016/j.toxlet.2010.03.907
P304-007 Mercury toxicokinetics during larval and juvenile stages in rainbow trout K. Kruzikova, R. Kensova, J. Havranek, Z. Svobodova University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic The aim of our study was to assess mercury content in tissues of rainbow trout (Oncorhychus mykiss) during the development stages in dependence on mercury content in fish feed. The measurement was conducted in feed, muscle, liver, kidney and in the fish homogenates every two months during one cycle of fish stock breeding. Atomic absorption spectrometry (analyzer AMA 254) was used for the determination. The total of 75 fish was used in the study. The mercury content increased during the breeding, the lowest content was detected in the whole body homogenate at the beginning of the experiment (4 ± 2 g/kg), whereas the highest mercury concentration was found in the muscle at the end of the experiment (128 ± 43 g/kg). The content of mercury in the liver and kidney did not differ significantly from the content in the muscle and ranged from 20 to 163 g/kg. Our results show a positive correlation between mercury level in fish and weight/length/age of fish. Three different feeds (A, B, C) were used in sequence during the rearing of rainbow trout. The levels of mercury were as follows: A = 12.6 gHg/kg,
B = 40.1 g/kg, C = 85.9 g/kg. Feeds A and C were used for one month at the beginning (A) and one month at the end (C) of the monitored period. Thus, for most of the experiment (16 months) the fish were supplied with feed B. Although the input of mercury (in feed) was practically the same during the 18 months, the mercury level increased in the muscle, liver and kidney of the fish. Acknowledgements: The study was supported by the project MSM 6215712402 “Veterinary aspect of food safety and quality”. doi:10.1016/j.toxlet.2010.03.908
P304-008 Aluminum analyses in Montrichardia linifera (Aninga), a wide range distributed Amazon macrophyte N. Santos Serrão De Castro 1 , A.L. Ferreira De Almeida Lins 2 , E. Conceic¸ão De Oliveira Santos 1 , I. Maura De Jesus 1 , C. Nahum Alves 3 , M. De Oliveira Lima 1 Evandro Chagas Institute, Brazil, 2 Museu Paraense Emilio Goeldi, Brazil, 3 Federal University of Pará, Brazil
1
Introduction: It is well documented that the Amazonia has a rich biodiversity and good mineral resources. In recent years, many mineral industries were attracted to the Amazonia, particularly to the city of Barcarena, Pará State, where an industrial complex of the aluminum production chain was installed. Objectives: To evaluate the Al concentration in root, shoot and leaf samples of Montrichardia linifera in four different rivers near industrial complex. Was quantified Al in sediments and physiochemical waters for environmental characterization. Methodology: The samples of M. linifera were collected, tissues were separated, washed with deionizer water, dried at 40 ± 5 ◦ C and homogenized. An amount of 200 mg for each sample were submitted to acid digestion (HNO3 + HClO4 ) and heated for 48 h (80 ± 5 ◦ C). The sediments were dried, particle separation (<270 mesh) and digested (HNO3 + HClO4 + HF), heated for 4 h (95 ± 5 ◦ C), centrifuged and supernatant solution was removed. Water physicochemical was performed by multiparameter probe and spectrophotometer analyses. Al analyses were performed through ICP-OES. GBW07604 and IAEA-SL-1 were used for quality control. Results: M. linifera collected in rivers near the industrial complex (Dendê, Curuperê and Murucupi) presented high concentrations of Al in roots and shoots, compared with the samples collected in Arapiranga (control). The analysis of Al sediments also indicates its high concentration in the rivers Dendê, Curuperê and Murucupi. Arapiranga presents the lowest concentrations of Al in sediments. Multivariate analysis demonstrated a positive correlation between sediments and shoots. Conclusions: M. linifera could survive at high Al concentration environments. Additionally, Al in roots and shoots reproduce clearly the profile of this metal in ambient. The physiochemical analysis supported the characterization of the outline of the rivers. This study indicates that industrial effluents are enhancing the naturally occurring Al concentration in sediments. Analysis in M. linifera reveals that the Al resulting from impacted areas is entering in ecosystem. doi:10.1016/j.toxlet.2010.03.909