- Email: [email protected]
Microdialysis monitoring - A method for monitoring organ and tissue chemistry
Abstracts
neurodegeneration, CamKII-TetOp25 mice (Tsai et al., 2003), to evaluate whether NF-L levels in CSF or blood can be used as a biochemical biomarker of neurodegeneration. Induction of p25 transgene brain expression led to increases in CSF and serum NF-L levels that correlated with increased neuronal stress and neurodegeneration. When p25 transgene expression was switched off this prevented further increases in both CSF and serum NF-L levels which correlated with the block of further increases in neuronal stress and neuronal loss. The levels of CSF NF-L detected in p25 mice are about 4–fold higher than the CSF levels detected in patients with chronic neurodegenerative diseases, such as symptomatic FTD (bvFTD). We also report increase with aging in CSF and plasma NFL in two other transgenic mouse models, ThyTau22 (Schindowski et al., 2006) and APPxPS1 mice (Balnchard et al., 2003). Finally, Western blot and NanoLC-MS/MS data indicate that the NF-L detected in CSF is most likely a cleaved form of NF-L. These results suggest that CSF and serum NF-L could be used as potential translational dynamic biomarkers of neurodegeneration in preclinical animal models and in clinical studies.
S15
This report demonstrate how bioinformatics approach on the example of using Elsevier's Pathway Studio® pathway analysis software can be used to create complex, predictive mechanistic models of biological processes, providing novel insights to researchers and helping them direct the course of their studies. The report will highlight application of bioinformatics and mechanistic models approach to biomarkers research. Presented examples include following: prediction of biomarkers based on reconstructed liver toxicity model, identification of new potential candidates for drug targets, identification of drug efficacy biomarkers or safety biomarkers and assessment of clinical biomarker specificity.
doi:10.1016/j.mgene.2018.05.061
Cancer biomarkers in biosensors fabrication for diagnostic purposes Samar Damiati
doi:10.1016/j.mgene.2018.05.059
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia E-mail address: [email protected]
Microdialysis monitoring - A method for monitoring organ and tissue chemistry Katarina Asberg
Abstract
M Dialysis AB, Stockholm, Sweden E-mail address: [email protected] Abstract Microdialysis is a technique for sampling the chemistry of individual organs and tissues. The basic principle is to mimic function of a capillary blood vessel by perfusing a thin semi-permeable dialysis membrane tube implanted in the tissue with a physiological solution. In the tissue substances from the interstitial fluid diffuse trough the dialysis membrane into the perfusion fluid. The perfusate is analyzed chemically and reflects the tissue or the organ chemistry. Today there are CE-approved Microdialysis catheters available for implantation in brain, liver, abdomen, muscle, subcutaneous tissue as well as intravenously. There are Microdialysis perfusion pumps, microvials and analyzers suitable for intra-operative as well as bedside use in the Intensive Care Unit. The presentation will give a brief overview of the different applications of clinical Microdialysis.
doi:10.1016/j.mgene.2018.05.060
Application of bioinformatics for biomarkers research, drug R&D and clinical practice Andrey Khudoshina,⁎, Anton Yuryevb a
Elsevier, Russia Elsevier, USA ⁎Corresponding author. E-mail address: [email protected] (A. Khudoshin)
b
Cancer is one of the leading causes of death worldwide and the number of deaths is increasing due to the limitations of the available diagnostic tests. Early diagnosis plays an important role in controlling disease progression and response to therapy. Hence, cancer biomarkers are widely exploited in oncology to detect the presence of tumors and to evaluate the subsequent treatment of patients. Biomarker-based biosensors have attracted attention due to their simplicity, rapidity, selectivity and sensitivity. The main obstacle that prevents the development of biosensors is the fact that cancer is a complex set of diseases and only a few biomarkers are exploited by oncologists to detect cancer. This talk will be focusing on the fabrication of biosensors for cancer biomarker detection and highlights various biomarker detection techniques. It will also discuss the limitations associated with some developed systems for immunoassay technology.
doi:10.1016/j.mgene.2018.05.062
Particles-induced osteoblast apoptosis and inflammation mediated by endoplasmic reticulum in a murine model of periprosthestic osteolysis Guoyin Liua,b,c,⁎, Yong Zhanga, Jin Wanga, Jiangning Chenc, Jianmin Chena, Junfeng Zhangc, Jianning Zhaob a
Bayi Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, China b Jinling Hospital affiliated to the School of Medicine, Nanjing University, China c School of Life Sciences, Nanjing University, China ⁎Corresponding author. E-mail address: [email protected] (G. Liu)
Abstract Abstract Liver toxicity is a key reason why new drugs fail in clinical trials, or once they are in broader use. Drug-induced cholestasis is a common form of liver toxicity. Yet currently there is no model or test to predict which drugs may induce cholestasis in patients.
Macrophage apoptosis and inflammation in interface membrane have been shown to play a significant role in the pathogenesis of osteolysis. However, osteoblasts have not been fully explored as a
neurodegeneration, CamKII-TetOp25 mice (Tsai et al., 2003), to evaluate whether NF-L levels in CSF or blood can be used as a biochemical biomarker of neurodegeneration. Induction of p25 transgene brain expression led to increases in CSF and serum NF-L levels that correlated with increased neuronal stress and neurodegeneration. When p25 transgene expression was switched off this prevented further increases in both CSF and serum NF-L levels which correlated with the block of further increases in neuronal stress and neuronal loss. The levels of CSF NF-L detected in p25 mice are about 4–fold higher than the CSF levels detected in patients with chronic neurodegenerative diseases, such as symptomatic FTD (bvFTD). We also report increase with aging in CSF and plasma NFL in two other transgenic mouse models, ThyTau22 (Schindowski et al., 2006) and APPxPS1 mice (Balnchard et al., 2003). Finally, Western blot and NanoLC-MS/MS data indicate that the NF-L detected in CSF is most likely a cleaved form of NF-L. These results suggest that CSF and serum NF-L could be used as potential translational dynamic biomarkers of neurodegeneration in preclinical animal models and in clinical studies.
S15
This report demonstrate how bioinformatics approach on the example of using Elsevier's Pathway Studio® pathway analysis software can be used to create complex, predictive mechanistic models of biological processes, providing novel insights to researchers and helping them direct the course of their studies. The report will highlight application of bioinformatics and mechanistic models approach to biomarkers research. Presented examples include following: prediction of biomarkers based on reconstructed liver toxicity model, identification of new potential candidates for drug targets, identification of drug efficacy biomarkers or safety biomarkers and assessment of clinical biomarker specificity.
doi:10.1016/j.mgene.2018.05.061
Cancer biomarkers in biosensors fabrication for diagnostic purposes Samar Damiati
doi:10.1016/j.mgene.2018.05.059
Institute for Synthetic Bioarchitectures, Department of Nanobiotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria Department of Biochemistry, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia E-mail address: [email protected]
Microdialysis monitoring - A method for monitoring organ and tissue chemistry Katarina Asberg
Abstract
M Dialysis AB, Stockholm, Sweden E-mail address: [email protected] Abstract Microdialysis is a technique for sampling the chemistry of individual organs and tissues. The basic principle is to mimic function of a capillary blood vessel by perfusing a thin semi-permeable dialysis membrane tube implanted in the tissue with a physiological solution. In the tissue substances from the interstitial fluid diffuse trough the dialysis membrane into the perfusion fluid. The perfusate is analyzed chemically and reflects the tissue or the organ chemistry. Today there are CE-approved Microdialysis catheters available for implantation in brain, liver, abdomen, muscle, subcutaneous tissue as well as intravenously. There are Microdialysis perfusion pumps, microvials and analyzers suitable for intra-operative as well as bedside use in the Intensive Care Unit. The presentation will give a brief overview of the different applications of clinical Microdialysis.
doi:10.1016/j.mgene.2018.05.060
Application of bioinformatics for biomarkers research, drug R&D and clinical practice Andrey Khudoshina,⁎, Anton Yuryevb a
Elsevier, Russia Elsevier, USA ⁎Corresponding author. E-mail address: [email protected] (A. Khudoshin)
b
Cancer is one of the leading causes of death worldwide and the number of deaths is increasing due to the limitations of the available diagnostic tests. Early diagnosis plays an important role in controlling disease progression and response to therapy. Hence, cancer biomarkers are widely exploited in oncology to detect the presence of tumors and to evaluate the subsequent treatment of patients. Biomarker-based biosensors have attracted attention due to their simplicity, rapidity, selectivity and sensitivity. The main obstacle that prevents the development of biosensors is the fact that cancer is a complex set of diseases and only a few biomarkers are exploited by oncologists to detect cancer. This talk will be focusing on the fabrication of biosensors for cancer biomarker detection and highlights various biomarker detection techniques. It will also discuss the limitations associated with some developed systems for immunoassay technology.
doi:10.1016/j.mgene.2018.05.062
Particles-induced osteoblast apoptosis and inflammation mediated by endoplasmic reticulum in a murine model of periprosthestic osteolysis Guoyin Liua,b,c,⁎, Yong Zhanga, Jin Wanga, Jiangning Chenc, Jianmin Chena, Junfeng Zhangc, Jianning Zhaob a
Bayi Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, China b Jinling Hospital affiliated to the School of Medicine, Nanjing University, China c School of Life Sciences, Nanjing University, China ⁎Corresponding author. E-mail address: [email protected] (G. Liu)
Abstract Abstract Liver toxicity is a key reason why new drugs fail in clinical trials, or once they are in broader use. Drug-induced cholestasis is a common form of liver toxicity. Yet currently there is no model or test to predict which drugs may induce cholestasis in patients.
Macrophage apoptosis and inflammation in interface membrane have been shown to play a significant role in the pathogenesis of osteolysis. However, osteoblasts have not been fully explored as a