Mitochondrial Biogenesis and Redox State in Gastrocnemius Muscle From Trained Rats Supplemented With L-ARGININE

421 The Difference in Nitric Oxide Content in Cyanobacteria Cultured in the Medium with And without Combined Nitrogen Fei-fei Li1, and Yang-Cang Xu1 1 Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin, China Background: Nitric oxide (NO) is a bioactive signaling molecule. Several potential NO sources may be distinguished in the plant organism, with the physiological role of each depending on the species, type of tissue or cells, external conditions and potential activation of the signal pathway in the plant. in this paper, the NO content in cyanobacteria cultured in the medium with and without combined nitrogen was studied. Results and Discussion: When cyanobacteria were cultured in medium with nitrate sodium, the nitric oxide content in Anabaena sp. PCC7120 was 560 nmol/g dry weight or 3546 fluorescent intensity/cell, and the nitric oxide content in Trichodesmium Erythraeum IMS101 was 780 nmol/g.dry weight or 5684 fluorescent intensity/cell. When cyanobacteria were cultured in medium without nitrate sodium for 3 h, the nitric oxide content in Anabaena sp. PCC7120 was 786 nmol/g dry weight or 5782 fluorescent intensity/cell, and in Trichodesmium Erythraeum IMS101 was 1542 nmol/g dry weight or 12383 fluorescent intensity/cell. It was distinct that the nitric oxide content increased in both cyanobacteria after removing nitrate sodium from medium. It had been shown that nitric oxide was mainly produced by NOSlike enzyme way or nitrate reducase /nitrite reducase way. When combined nitrogen was removed from medium, both L-arginine and nitrite ion content in cyanobacteria decreased, however, the nitric oxide content increased. Then where the nitric oxide come from? the experiment is currently ongoing.

combination of physical exercise plus L-arginine supplementation promoted a significant increase in proteins expression of mtTFA (TR/Larg: 0.89±0.05) compared to C/SD (0.53±0.04); SD/Larg (0.52±0.08) and TR (0.77±0.07); Mn-SOD (TR/Larg: 1.84±0.1) compared with C/SD (1.16±0.1); SD/Larg (0.97 ±0.1) and TR (0.92 ±0.07); and nNOS (TR/Larg: 1.2±0.09) compared with C/SD (0.6±0.1); SD/Larg (0.7±0.1), TR (0.8±0.1) from rat gastrocnemius muscle. However, protein expression of ATP sintase C was not modified in all groups. Conclusion: Exercise training in combination with L-arginine supplementation showed more efficacy on mitochondrial biogenesis in skeletal muscle which was positively related to increased expression of mitochondrial MnSOD and nNOS. Financial support: FAPESP

doi:10.1016/j.freeradbiomed.2011.10.239

doi:10.1016/j.freeradbiomed.2011.10.238

422 Mitochondrial Biogenesis and Redox State in Gastrocnemius Muscle From Trained Rats Supplemented With L-arginine Angelina Zanesco1, Carmem Peres Silva1, Maria Andreia Delbin1, Paolo G La Guardia2, Ana Paula Davel2, and Fernanda B Priviero1 1 2 UNESP, UNICAMP Nitric oxide (NO) plays an important role in the mitochondrial biogenesis by activating several transcription factors. Further, physical exercise exerts beneficial effects on the mitochondrial biogenesis affecting energy metabolism. Objective: Therefore, we tested the hypothesis that exercise training in combination with L-arginine supplementation would act synergistically on the mitochondrial biogenesis in gastrocnemius muscle from male Wistar rat. We also evaluated glycemia, lipd profile and redox state. Methods: Exercise training was performed for 5 days/week, session of 60 min, at a speed of 15 meters/min. L-arginine was given by gavage (62.5 mg/ml/day/rat). L-arg supplementation alone (SD/Larg) or associated with exercise program (TR/Larg) lasted 8 weeks. Blood glucose, lipid profile and malondialdehyde (MDA) were measured by commercial kits. Protein expressions of mitochondrial transcription factor a (mtTFA), ATP sintase C, Cu/Zn-superoxide dismutase (Cu/Zn-SOD), Mn-superoxide dismutase (Mn-SOD), neuronal NO synthase (nNOS) were measured in mitochondria from gastrocnemius muscle. Results: There were a significant decrease in triglyceride levels in both TR and TR/Larg groups whereas HDL-C was increased only in TR/LArg group compared with C/SD, SD/Larg and TR groups. No changes were found in glycemia and MDA levels in all groups. the

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