Mo-P1:158 Apolipoprotein E polymorphism in native population of Mountain Shoriya (West Siberia) and its association with plasma lipid levels

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Mon&ty, June 19, 2006: Poster Session Epidemiology of cardiovascular disease

Conclusions: CVD risk profile in the elderly shows a high prevalence of modifiable RF. Mean value and prevalence of RF increases with age, resulting in a high burden of risk. This population requires a global risk approache and s p e d a l strategies for prevention in CVD. I

I Mo-P 1 1581 A P O L I P O P R O T E I N E P O L Y M O R P H I S M IN N A T I V E i P O P U L A T I O N OF M O U N T A I N S H O R I Y A ( W E S T SIBERIA) AND ITS ASSOCIATION WITH PLASMA LIPID LEVELS E.V. Shakhtshneider, O.L. Baxbaxash, M.Y. Ogarkov, I.V. Kulikov, V.A. Baum, S.N. Ustinov, N.S. Yudin, V.F. Kobzev, A.G. Romashchenko, M.I. Voevoda. btstitute of bttemal Medicine, Novosibirsk, Russia Objective: we investigated apolipoprotein E gene polymorphism and its influence on a plasma lipid levels in native population of Mountain Shoriya (West Siberia). M e t h o d s : The study included 40 men and 80 women aged 25-64. The apolipoprotein E polymorphism was analyzed by original method using Hixson's approach. The plasma lipid levels were determined by standard enzymatic assays. Results: The frequencies of E2 (Cys112Cys158), E3 (Cysl12Arg158), E4 (Argl12Arg158) alleles in men were 6.5%, 80.4%, 13.1% and in women - 7.6%, 72.6%, 19.8% respectively. The frequencies of genotypes E2/E4, E2/E3, E3/E3, E3/E4, E4/E4 in men and women together were 1.8%, 12.4%, 51.8%, 31.2% and 2.9%. Mean total serum cholesterol levels in case of genotypes E2/E4, E2/E3, E3/E3, E3/E4 and E4/E4 were 282.9-t-29.7 mg/dl, 221.1-t-10.8 mg/dl, 189.1-t-5.4 mg/dl, 228.1-t-9.2 mg/dl, 326.2-t-23.6 mg/dl (pANOVA=0,0001). The differences of triglycerides and high density lipoproteins cholesterol levels between genotypes are not significant. Conclusions: The native population of Mountain Shoriya is chaxacterized by relatively high frequencies of E4 alleles. The allele E4 prevails in women. The genotypes E2/E4, E3/E4 and E4/E4 have been associated with higher total serum cholesterol level.

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I Mo-P 1 159 iI D I S T R I B U T I O N OF O C H R O N O T I C P I G M E N T IN THE CARDIOVASCULAR SYSTEM - A REAPPRAISAL L.R. Ranganath, T. Helliwell. Universi~ of Liverpool, Liverpool, United

Kingdom I n t r o d u c t i o n : Alkaptonuria (AKU), an autosomal recessive genetic deficiency of homogentisic acid oxidase, results in accumulation of homogentisic acid (HGA) and its polymeric pigment benzoquinone-acetic acid (BAA). Polymeric BAA, derived by the oxidation of HGA, binds to connective tissue resulting in the characteristic ochre pigmentation. Clinical and post-mortem studies in A K U indicate that the deposition of BAA in the cardiovascular system (CVS) is associated with the fibrous component of atherosclerotic plaques and with clinical aortic valve stenosis. Aims and Methods: To document the pattern of pigmentation in the CVS during the post-mortem examination of a 74-year old female with AKU who died from disseminated ovarian carcinoma. Results: Atheromatous plaques were noted in the aorta, coronary, renal, carotid and cerebral arteries. The following features were noted in the distribution of pigmentation: (1) The intimal pigmentation was patchy and more prominent around branches and bifurcations of major arteries. (2) The adventitia of the aorta and carotid arteries were also pigmented to varying degree but not the media. (3) Prominent pigmentation was noted in the aortic and mitral valve rings and cusps. (4) Little pigmentation was seen in the right heart and pulmonary artery. (5)The venous system appeared normal. Conclusion: Pigmentation of CVS was not uniform and although the reason(s) axe unknown, we speculate whether the variation in oxidation may explain this at least in part. F u n d i n g : The Alkaptonuria Society of United Kingdom supported this study. I

I Mo-P1 1601 i A S U R V E Y O N K N O W L E D G E OF C H I N E S E P H Y S I C I A N IN P R E V E N T I O N A N D C O N T R O L OF DYSLIPIDEMIA J.Y. Li 1 , D.Y. Hu 2, X.B. Shi 2, Q.G. Tong 2. 1Tongji Hospital of Tongji

Universi~, Shanghai, China: 2Tongren Hospital Affiliated To Capital Universi~ of Medical Science, Beijing, China Objective: To understand Chinese physicians current knowledge in preven-

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tion and control of dyslipidemia and to provide guidance to China cholesterol education program. M e t h o d s : Through the use of a questionnaire on dyslipidemia, 4000 physicians from 23 metropolitan cities in China were evaluated. 3054 subjects have complete data for analysis. Results: Only 87.1% of respondents know the treatment goal of LDL-C in coronary heart disease. 56.7% of physicians would examine the patients" lipid profile in consistence with Chinese guideline. There axe 32.4% of doctor who don't know the primary target of lipid-modifying.26.4% of doctors diagnose the dyslipidemia only according to the reading in the assay report. 57.8% of participants answer that he or she would ask for a new lipid profile during the first month after the treatment 20.5% of doctors would reduce the dose or discontinue the drug therapy once the required lipid levels axe attained. The lower the level of hospital they served, the worse the above status in physicians. C o n d u s i o n s : There is a gap in the participating physicians'knowledge in prevention and control of dyslipidemia compared to the ATP III and Chinese National guideline. Chinese Cholesterol Education should be stressed. I

Mo-P1:161 Ii D I F F E R E N C E S IN R I S K F A C T O R S A N D ANGIOGRAPHIC FINDINGS BETWEEN THREE E T H N I C G R O U P S OF C A R I B B E A N P A T I E N T S W I T H LOWER LIMB ARTERIAL DISEASE M. Deneuville, F. Losy. Department of Vascular and Thoracic Surgery,

Universi~ Hospital ( CHU) of GucMeloupe, Pointe-gt-Pitre, France Objective: The aim of this study was to compare common risk factors and anatomical involvement of lower extremity arterial disease (LEAD) in different ethnic groups. M e t h o d s : Data was prospectively collected from patients referred for symptomatic LEAD. Patients were of African [Aft, n=196], European [Eu,n=67] or Indian [Ind, n=60] ascent. Scores of severity graded 0-3 were blindly established on angiography by two independent observers and averaged for the aorto-iliac, femoro-popliteal, crural and foot levels. Results: Severity scores (ANOVA) were significantly higher for Eu at aortic level (1.8 4- 2.1) vs Ind (0.93 4- 1.6) and Aft (0.44 4- 1.2). At crural level, scores were significantly higher for Aft (1.94 4- 0.6) vs Ind (1.63 4- 0.7) and Eu (1.28 4- 0.8). Significant differences in risk factors were also observed between ethnic groups; diabetes and hypertension were more common in Indians (82 and 77%, respectively) and Africans (90 and 55%) compared to Eu (56 and 30%). Smoking was more common in Eu (81%) compared to Ind (47%) and Aft (30%). LDL-cholesterol rates were similar between groups. The sex ratio (male:female) was 8.5 in Eu, 2.8 in Ind and 1.3 in Aft. C o n d u s i o n s : This study indicates ethnic differences in both risk factors for atherosclerosis and angiograpic patterns, notably a more severe involvement at the infrapopliteal arteries in patients of african origin uncleaxly related to diabetes. Our results suggest a possible link between proximal involvement of L E A D and smoking currently uncommon in African and women. These findings have obvious implications in public health in the West Indies.

Mo-P1:162 I F A M I L I A L HYPERCHOLESTEROLEMIA-SCREENING RELATIVES

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K. Gkogkos, G. Pavlidis, A. Karakozoglou, S. Fragidis, A. Maliouri, J. Kountouras. G.Papanicolaou Hospital, B 'b~tental Medicine Department,

Thessaloniki, Greece Objective: Familial Hypercholesterolemia(FH)is an autosomal dominant hereditary disease.Its frequency is estimated to be 1 in 500 and considered to be an important predisposing factor for premature Coronary Artery Disease. Manifestations of the disease in heterozygotes-except elevated total cholesterol(two to three fold increase)include tendon xanthomata, xanthelasmata and senile axcuses,which axe quite chaxacteristic.The aim of this study is to search and find relatives of patients with FH afflicted with the same disease. M e t h o d s : Lipid profile and appropriate physical examination were performed in 146 relatives of 28 patients with FH,searcing specifically for elevated total cholesterol,tendon xanthomata and xanthelasmata, symptoms and signs suggestive of Coronary Artery Disease. Results: 78 out of 146 people,ranging from 25 to 65 years old were found to have hypercholesterolemia consistent with the diagnosis of FH.45 out of 78 were not aware of their problem.16 had family history of Coronary Artery Disease,with the younger one being 36 years old.24 of them were under

XIV bztentational Symposium on Atherosclerosis, Rome, Italy, June 18-22, 2006