Modifying effect of ginger on mutagenicity in pan fried chicken

JEMSAbstracts/Mutation Research334 (1995)385-427 typhimurium TA1535 and YG71 series with deficient strains of O6-alkylguanine alkyltransferase (AGT), and the mechanism of the inhibition of mutagenicity was examined in relation to the DNA alkylations and their repair. DNA methylation by NMU was repaired with AGT produced by both ada and ogt genes. However, ethylation, propylation and butylation were repaired with AGT produced only by the ogt gene. Acetate buffer inhibition of mutagenicity with NMU was strongest, and only weaker inhibition was observed with N-nitrosoalkylureas with longer alkyl chains. A sudden increase in the dose-mutagenicity profiles of NMU and NEU was explained by increased repair induced by acetate buffer. However, since inhibition was detected by acetate buffer in NMU and NEU mutagenicity in YG7108 (Aada, Aogt), and also in the mutagenicity of NPU and NBU, it was estimated that repair systems existed other than AGT, which are related to the inhibition of the mutagenicity by acetate buffer. 51 Muraoka, N., N. Uchida, Y. Hosoi and M. Aoki, Department of Food Science and Nutrition, Sanyo Gakuen Junior College, 1-14-1 Hirai, Okayama 703, Japan Modifying effect of ginger on mutagenicity in pan fried chicken

In a previous study, we determined mutagenicities of Japanese meals by the blue cotton method coupled with the Ames test (S. typhimurium TA98, + $9). A series of typical modern Japanese meals were included in this survey. We observed that the mutagenicities of cooked meat samples were significantly different depending on the seasonings added before cooking. In this paper we investigated the effect of seasoning with ginger on mutagenicity. Lean chicken cut into 1 cm long pieces with or without added seasoning was fried in a pan for 3 min. The cooked meat was homogenized and the homogenate was extracted with 0.1 N HCI, centrifuged, and the neutralized supernatant was extracted with blue cotton. Mutagenicities (TA98, + $9) of the blue cotton extracts were determined

407

to be (revertants/50 g raw meat) 824 (no seasoning), 1056 (soy sauce, sake, sugar; teriyaki) and 1338 (ground ginger, soy sauce, sake, sugar; shogayaki). Thus, ginger seems to have an enhancing effect on mutagen formation. We fractionated ginger by solvent extraction and found that an ethyl acetate soluble fraction produces the enhancing effect. Interestingly, after ethyl acetate extraction a water soluble fraction showed a strong inhibitory effect on mutagen formation (234 revertants/50 g, with 2.5 g ginger equivalent). Supported by Ryobi Teien Foundation. 52 Nagaoka, K., T. Sato, H. Nagase, H. Kito, M. Niikawa, K. Suzuki and H. Takagaki. Department of Public Health, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu City 502, Japan Modifying effects of indomethacin and Shosaikoto on mutations induced by an anticancer medicine, mitomycin C

We were interested in studying the effects of combining an anticancer medicine with other medicines prescribed simultaneously. So, we examined medicines which modify the mutagenicity of an anticancer medicine, mitomycin C (MMC), using the wing spot test of Drosophila melanogaster. The mutagenicity of MMC was dose-dependent as measured by the wing spot test; small single spots (SSS), large single spots (LSS) and twin spots (TS) were 7.2, 81 and 32 times of those without MMC at 0.003 mg/bottle. SSS, LSS and TS induced by 0.003 mg/bottle of MMC were over 60% suppressed by the simultaneous administration of indomethacin at 20 mg/bottle in contrast, MMC-induced spots were enhanced 35% by Shosaiko-to at 50 mg/bottle. SSS, LSS and TS were also suppressed when the larvae were exposed to MMC first, and then washed to eliminate MMC and exposed to indomethacin. Therefore, the suppressing effects of indomethacin are considered to be bio-antimutagenie.