- Email: [email protected]
Modulation of cold shivering response by nucleus raphe magnus and subcoeruleus in anesthetized rats
s225 Id-49
MODULATION OF COLD SHIVERING RESPONSE BY NUCLEUS RAPHE MAGNUS AND SUBCOERULEUS IN ANESTHETIZED RATS. HARUHIKO SAT-O, Department of Physical EduChu&o Women's University, 55 Nadakayama, cation_,_Faculty of Physical Education,
Yokone-cho,
_@?42!lichlX-!!Japa!
The activations of motor units induced by whole body cooling were analyzed (GAS) and tibialis anterior as 'a shivering response' (SR) in the gastrocnemius muscles (TA) of the Nembutal anesthetized rats, ana whether these SR are affecTed by electrical stimulation in the nucleus raphe magnus (NRM) and subcoeruleus (SC) The rats were stereotaxically fixed in a small climatic or not wer-e investigated. chamber, and the rectal temperature was maintained at a constant level by interOnce rectal temperature was mittently administering a small amount of Nembutal. the SR induced repeatedly were quite reproducible. A marked enhan:estabilized, ment of the SR in the GAS was produced by NRM stimulation and in the TA by SC stimulation. From correspondence with the effects of NRM and SC stimulation on fusimotor activity obtained previously, it was suggested that the facilitation of the SR in the GAS from the NRM and in the TA from the SC could be ascribed to dynamic and static fusimotor activation, respectively.
14-50 ARISING
ASSOCIATION
AND COMMISSURAL
FROM THE SUPERIOR
ANTEROGRADE
VESTIBULAR
PROJECTIONS NUCLEUS
OF THE VESTIBULAR
IN THE ALBINO
NUCLEAR
RAT USING
COMPLEX
BIOCYTIN
AS AN
TRACER. EUMETANI. TAKEHIK
7-Chome. Kusunoki-cho.
Chuo-ku. Kobe 650. Japan
Courses and terminations vestibular projections
of association and commissural
(SVN) were investigated
fibers of the vestibular complex arising from the superior
in the albino rat by a method using an anterograde transport of extracellularly
injected biocytin. Ipsilaterally, the SVN projected to the ventral part of the lateral vestibular nucleus (LVN) and to the spinal (SpVN) and medial vestibular nuclei (MVN). Small cell group of the complex, subnuclei f, x and y and infracerebellar and interstitial nuclei, also received the ipsilateral projections. Contralateral fibers arising from the dorsal part of the SVN passed through the ipsilateral juxtarestiform body, crossed the midline in the cerebellar commissure and projected to the dorsal part of the SVN, the ventral part of the LVN, the dorsolateral part of the MVN and sparsely to the SpVN. On the other hand, those fibers arising from the ventral part of the nucleus crossed the midline close to the medial longitudinal fascicle at a level of the rostral pole of the SVN to project to the ventral parts of the SVN, LVN and SpVN, and the ventrolateral part of the MVN.
14-51
RETICULOSPINAL PROJECTIONS FROM THE PONTINE RETICULAR FORMATION IN THE CAT, RELATED TO CONTROL OF NECK, AXIAL AND LIMB MOVEMENTS. KIYOJI MATSUYAMA. YOSHIFUMI KOBAYASHI. KAORU TAKAKUSAKI AND SHIGEMI MORI. Deoartment of Phvsiolouv. Asahikawa Medical Colleee. 4-S. Nishikauura, Asahikawa 078. Janan.
In this study, attempts were made to investigate the descending projections from the medial part of the pontine reticular formation, corresponding to the rostra1 half of the gigantocellular tegmental field (FlG) defmed by Berman, to the cervical, upper thoracic and lumbosacral cord related to control of neck, axial and limb movements. For this purpose, an anterogmde neural tracer, PHA-L was focally injected into pontine FIG in cats (n=4) to elucidate the details of the descending axonal trajectories of FTG cells including the fine morphology of fibers and their terminal locations in the spinal cord. The post-injection survival periods of animals were 8 to 9 weeks. Descending axonal trajectories from pontine FTG could be subdivided into two subgroups. The first group of axons with thick calibers descended throughout the length of the spinal cord via the ventral and ventrolateral funiculi, ipsilatemlly. These axons sent collaterals to the gray matter along their descending course from the upper cervical to the lumbosacral cord. In the gray matter, PHA-L-positive fibers and terminals were distributed densely to laminae VII and VIII, where intemeurons innervating neck, axial and limb motoneurons were located, with an ipsilateral predominance. There were additional fibers and terminals distributed to the motoneuron pools of neck and axial muscles, and some terminals were closely apposed to motoneuronal somata. Few labelings were observed in the motoneuron pools of limb muscles at the cervical and lumber enlargements. The second group of axons with fine calibers descended only until the level of C5 via the ventral and ventrolateral fumculi, bilaterally. These axons gave off fibers to the inter- and motoneuron pools of neck muscles at the upper cervical cord. These results suggest that pontine reticulospinal control of neck and axial movements differs from that of limb movements.
MODULATION OF COLD SHIVERING RESPONSE BY NUCLEUS RAPHE MAGNUS AND SUBCOERULEUS IN ANESTHETIZED RATS. HARUHIKO SAT-O, Department of Physical EduChu&o Women's University, 55 Nadakayama, cation_,_Faculty of Physical Education,
Yokone-cho,
_@?42!lichlX-!!Japa!
The activations of motor units induced by whole body cooling were analyzed (GAS) and tibialis anterior as 'a shivering response' (SR) in the gastrocnemius muscles (TA) of the Nembutal anesthetized rats, ana whether these SR are affecTed by electrical stimulation in the nucleus raphe magnus (NRM) and subcoeruleus (SC) The rats were stereotaxically fixed in a small climatic or not wer-e investigated. chamber, and the rectal temperature was maintained at a constant level by interOnce rectal temperature was mittently administering a small amount of Nembutal. the SR induced repeatedly were quite reproducible. A marked enhan:estabilized, ment of the SR in the GAS was produced by NRM stimulation and in the TA by SC stimulation. From correspondence with the effects of NRM and SC stimulation on fusimotor activity obtained previously, it was suggested that the facilitation of the SR in the GAS from the NRM and in the TA from the SC could be ascribed to dynamic and static fusimotor activation, respectively.
14-50 ARISING
ASSOCIATION
AND COMMISSURAL
FROM THE SUPERIOR
ANTEROGRADE
VESTIBULAR
PROJECTIONS NUCLEUS
OF THE VESTIBULAR
IN THE ALBINO
NUCLEAR
RAT USING
COMPLEX
BIOCYTIN
AS AN
TRACER. EUMETANI. TAKEHIK
7-Chome. Kusunoki-cho.
Chuo-ku. Kobe 650. Japan
Courses and terminations vestibular projections
of association and commissural
(SVN) were investigated
fibers of the vestibular complex arising from the superior
in the albino rat by a method using an anterograde transport of extracellularly
injected biocytin. Ipsilaterally, the SVN projected to the ventral part of the lateral vestibular nucleus (LVN) and to the spinal (SpVN) and medial vestibular nuclei (MVN). Small cell group of the complex, subnuclei f, x and y and infracerebellar and interstitial nuclei, also received the ipsilateral projections. Contralateral fibers arising from the dorsal part of the SVN passed through the ipsilateral juxtarestiform body, crossed the midline in the cerebellar commissure and projected to the dorsal part of the SVN, the ventral part of the LVN, the dorsolateral part of the MVN and sparsely to the SpVN. On the other hand, those fibers arising from the ventral part of the nucleus crossed the midline close to the medial longitudinal fascicle at a level of the rostral pole of the SVN to project to the ventral parts of the SVN, LVN and SpVN, and the ventrolateral part of the MVN.
14-51
RETICULOSPINAL PROJECTIONS FROM THE PONTINE RETICULAR FORMATION IN THE CAT, RELATED TO CONTROL OF NECK, AXIAL AND LIMB MOVEMENTS. KIYOJI MATSUYAMA. YOSHIFUMI KOBAYASHI. KAORU TAKAKUSAKI AND SHIGEMI MORI. Deoartment of Phvsiolouv. Asahikawa Medical Colleee. 4-S. Nishikauura, Asahikawa 078. Janan.
In this study, attempts were made to investigate the descending projections from the medial part of the pontine reticular formation, corresponding to the rostra1 half of the gigantocellular tegmental field (FlG) defmed by Berman, to the cervical, upper thoracic and lumbosacral cord related to control of neck, axial and limb movements. For this purpose, an anterogmde neural tracer, PHA-L was focally injected into pontine FIG in cats (n=4) to elucidate the details of the descending axonal trajectories of FTG cells including the fine morphology of fibers and their terminal locations in the spinal cord. The post-injection survival periods of animals were 8 to 9 weeks. Descending axonal trajectories from pontine FTG could be subdivided into two subgroups. The first group of axons with thick calibers descended throughout the length of the spinal cord via the ventral and ventrolateral funiculi, ipsilatemlly. These axons sent collaterals to the gray matter along their descending course from the upper cervical to the lumbosacral cord. In the gray matter, PHA-L-positive fibers and terminals were distributed densely to laminae VII and VIII, where intemeurons innervating neck, axial and limb motoneurons were located, with an ipsilateral predominance. There were additional fibers and terminals distributed to the motoneuron pools of neck and axial muscles, and some terminals were closely apposed to motoneuronal somata. Few labelings were observed in the motoneuron pools of limb muscles at the cervical and lumber enlargements. The second group of axons with fine calibers descended only until the level of C5 via the ventral and ventrolateral fumculi, bilaterally. These axons gave off fibers to the inter- and motoneuron pools of neck muscles at the upper cervical cord. These results suggest that pontine reticulospinal control of neck and axial movements differs from that of limb movements.