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Modulation of intracellular gene expression toward Th2 predominant profile by monosodium glutamate in Jurkat cell line
S214 Abstracts
J ALLERGY CLIN IMMUNOL FEBRUARY 2004
754
Modulation of Intracellular Gene Expression Toward Th2 Predominant Profile by Monosodium Glutamate in Jurkat Cell Line
MONDAY
J. Shin1, Y. Seo2, N. Cho3, G. Noh4; 1Pediatric Allergy, Joey Children’s Hospital, Taejeon, REPUBLIC OF KOREA, 2Molecular Immunology, Seoul Allergy Research Institute, Food BioTech Co.Ltd, Seoul, REPUBLIC OF KOREA, 3Microarray & Molecular Immunology, Seoul Allergy Research Institute, Food BioTech Co.Ltd, Seoul, REPUBLIC OF KOREA, 4Allergy & Clinical Immunology, Seoul Allergy Clinic, Seoul, REPUBLIC OF KOREA. RATIONALE: Immunoregulatory roles of food additives are not well known. MSG is widely used in cooking. Immunoregulatory roles of MSG by affecting intracellular signal transduction through modulation of Th1and Th2-related gene expressions was investigated in this study. METHODS: Four conditions of Jurkat cells were made including nonstimulation (NS), PHA stimulation (PS), MSG stimulation(MS), and costimulation with PHA and MSG (CS). Total RNA’s were extracted from 1x106 cells of every condition at 0, 1, 4, 8, and 24 hours after stimulation. RT-PCR was performed and Allergy Gene Chip (AGC) was used for the analysis of Th1- and Th2-related gene expression were tested. RESULTS: MSG affects gene expression relating with Th1- and Th2-signal transduction toward resultant Th2 predominant status. Especially, the expression of t-bet and stat4 were decreased by co-stimulation of Jurkat cell with PHA+MSG. However, The expression of GATA3 were increased by co-stimulation of Jurkat cell with PHA+MSG. CONCLUSIONS: MSG modulated intracellular gene expression toward Th2 predominant profile and MSG may have immunomodulatory effects of Th2-deviation to be prone to allergic status.
J ALLERGY CLIN IMMUNOL FEBRUARY 2004
754
Modulation of Intracellular Gene Expression Toward Th2 Predominant Profile by Monosodium Glutamate in Jurkat Cell Line
MONDAY
J. Shin1, Y. Seo2, N. Cho3, G. Noh4; 1Pediatric Allergy, Joey Children’s Hospital, Taejeon, REPUBLIC OF KOREA, 2Molecular Immunology, Seoul Allergy Research Institute, Food BioTech Co.Ltd, Seoul, REPUBLIC OF KOREA, 3Microarray & Molecular Immunology, Seoul Allergy Research Institute, Food BioTech Co.Ltd, Seoul, REPUBLIC OF KOREA, 4Allergy & Clinical Immunology, Seoul Allergy Clinic, Seoul, REPUBLIC OF KOREA. RATIONALE: Immunoregulatory roles of food additives are not well known. MSG is widely used in cooking. Immunoregulatory roles of MSG by affecting intracellular signal transduction through modulation of Th1and Th2-related gene expressions was investigated in this study. METHODS: Four conditions of Jurkat cells were made including nonstimulation (NS), PHA stimulation (PS), MSG stimulation(MS), and costimulation with PHA and MSG (CS). Total RNA’s were extracted from 1x106 cells of every condition at 0, 1, 4, 8, and 24 hours after stimulation. RT-PCR was performed and Allergy Gene Chip (AGC) was used for the analysis of Th1- and Th2-related gene expression were tested. RESULTS: MSG affects gene expression relating with Th1- and Th2-signal transduction toward resultant Th2 predominant status. Especially, the expression of t-bet and stat4 were decreased by co-stimulation of Jurkat cell with PHA+MSG. However, The expression of GATA3 were increased by co-stimulation of Jurkat cell with PHA+MSG. CONCLUSIONS: MSG modulated intracellular gene expression toward Th2 predominant profile and MSG may have immunomodulatory effects of Th2-deviation to be prone to allergic status.