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Molecular and ultrastructural aspects in invasive mammary carcinoma
Abstracts / Journal of Biotechnology 231S (2016) S4–S109
focused on the optimization of environmental conditions for bacterial activity in the presence of crude oil. It attributed to both, production of microbial biomass and its introduction to the contaminated soil/water. First, experiments on adaptation of bacterial culture to the hydrocarbons during serial inoculations have revealed the preferences among different hydrocarbons, e.g., diesel oil, paraffin, etc. Second, immobilization of cells on the ceramic support was performed. Alterations in cell growth, physiology and metabolic activity may be induced by cell immobilization. A selection of the most appropriate types of ceramic beads for bacteria immobilization was made. Besides, the beads with a density below 1 g cm−3 were fabricated and tested for capturing the floating oils. The main advantages of immobilized biomass are as follows: retention of higher concentrations of microorganisms, protection of cells against toxic substances and prevention of suspended bacterial biomass in the effluent. Moreover, immobilization of microbial cells provides, in general, high degradation efficiency and good operational stability. Third, composition of nutrient amendments added to media plays a crucial role in the biodegradation process. Different nutrient formulations were tested in order to enhance the microbial viability and activity. Acknowledgement: Research was financially supported by the Latvian National Program 2014.10-4/VPP-6/6 ResProd. http://dx.doi.org/10.1016/j.jbiotec.2016.05.322 Population health maintenance by using agro-alimentary education projects in Romania Gheorghe Pribeanu 1 , Ionut Aurel Balan 1,∗ , Ionela Mihaela Milutin 2 , Cosmina Simona Toader 1 , Simona Cristina Constantinescu 1 , Elena Tonea 1 1
Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania 2 West University of Timisoara, Timisoara, Romania E-mail address: [email protected] (I.A. Balan). In recent years, food industry introduced in the daily menu many non-natural products to which we cannot resist. Many substances from the agro-alimentary products like additives and preservatives represent a real danger for the human health. A Romanian expression says “Tell me what you eat to tell you who you are!” This saying shows a significant truth that there is a strong relationship between diet and health. An analysis of the food consumption helps us to realize the profile of the consumer in terms of life style, agro-alimentary education and importance paid to the diet and health. An important role in ensuring and maintaining the population health is played by the educational environment which informs the consumers of agro-alimentary products about the benefits of a correct consumption and of bio-ecological origin. We need to ensure the educational framework related to the agro-alimentary area, to train the population in order to help them stimulate the health parameters, to be aware of the benefits on the human health. In the present days many countries focus on the health diet, but this means an active involvement of the decisional factors and a considerable financial effort. In Romania by the involvement of different actors (authorized institutions, NGO’s) there are projects in the educational institutions, campaigns which help educating the children in terms
S91
of healthy diet (“Milk and croissant”, “Fruits in schools”, “Health begins in the plate”). http://dx.doi.org/10.1016/j.jbiotec.2016.05.323 Molecular and ultrastructural aspects in invasive mammary carcinoma Corina Elena Mihalcea 1,∗ , Liliana Puiu 1 , Ana Maria Morosanu 2 , Daniela Murarasu 1 , Silviu Voinea 1 , Sabin Cinca 1 , Nicolae Mirancea 2 1
Institute of Oncology, Department Carcinogenesis and Molecular Biology, Bucharest, Romania 2 Institute of Biology, Romanian Academy, Department Plant and Animal Cytobiology, Bucharest, Romania E-mail address: corina [email protected] (C.E. Mihalcea). Mammary carcinoma is the most non-skin malignancy in women. More than 95% of breast cancers are adenocarcinomas which are divided into in situ- or invasive-carcinoma. Based on similarities in the gene expression profile, breast cancers can be also classified into distinct subgroups, useful to establish appropriate therapeutic protocol. Here we present some peculiar aspects concerning infrastructure of tumor cells involved in invasive process, especially desmosomal junctions, invadopodia and shedding membrane vesicles. Moreover, we report about new described cell phenotype termed telocytes involved in cell signaling by their homo- and hetero-cellular contacts. Telocytes from mammary tumor stroma exhibit a reduced number of hetero-cellular contacts, which suggests a possible perturbation of tissue homeostasis modulation. Data on structural aspects were correlated with studies on PIK3CA mutations – gene involved in proliferation, differentiation, and cell survival, that revealed a high incidence (87.5%) of hot spot mutations, most of them located in the kinase domain; in this regard, there were determined: the frequency of mutations in exons 1, 9 and 20 of the PIK3CA gene, gene copy number variation and the relationship between somatic mutations and gene amplification in breast tumors. Acknowledgment: The work was financed by project Partnership no 4/2012, UEFISCDI, Romania. http://dx.doi.org/10.1016/j.jbiotec.2016.05.324 Obtaining vegetable juices with high nitrite concentration using Staphylococcus xylosus ATCC 29971 strain as source of nitrate reductases Sabina Blascu ∗ , Corina Predescu, Camelia Papuc, Valentin Nicorescu, Constantin Savu University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: blascu [email protected] (S. Blascu). Vegetables naturally accumulate high amounts of nitrate. The amount of nitrate in plants varies among plant species and even among parts of the same plant (roots, leaves). Staphylococcus xylosus is a microorganism able to produce nitrate reductases that convert nitrate to nitrite. The aim of this study was to obtain the highest concentration of nitrites by reduction of nitrates from Iceberg and Romaine lettuce juices in the presence of nitrate reductases produced by S. xylosus ATCC 29971 strain.
focused on the optimization of environmental conditions for bacterial activity in the presence of crude oil. It attributed to both, production of microbial biomass and its introduction to the contaminated soil/water. First, experiments on adaptation of bacterial culture to the hydrocarbons during serial inoculations have revealed the preferences among different hydrocarbons, e.g., diesel oil, paraffin, etc. Second, immobilization of cells on the ceramic support was performed. Alterations in cell growth, physiology and metabolic activity may be induced by cell immobilization. A selection of the most appropriate types of ceramic beads for bacteria immobilization was made. Besides, the beads with a density below 1 g cm−3 were fabricated and tested for capturing the floating oils. The main advantages of immobilized biomass are as follows: retention of higher concentrations of microorganisms, protection of cells against toxic substances and prevention of suspended bacterial biomass in the effluent. Moreover, immobilization of microbial cells provides, in general, high degradation efficiency and good operational stability. Third, composition of nutrient amendments added to media plays a crucial role in the biodegradation process. Different nutrient formulations were tested in order to enhance the microbial viability and activity. Acknowledgement: Research was financially supported by the Latvian National Program 2014.10-4/VPP-6/6 ResProd. http://dx.doi.org/10.1016/j.jbiotec.2016.05.322 Population health maintenance by using agro-alimentary education projects in Romania Gheorghe Pribeanu 1 , Ionut Aurel Balan 1,∗ , Ionela Mihaela Milutin 2 , Cosmina Simona Toader 1 , Simona Cristina Constantinescu 1 , Elena Tonea 1 1
Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara, Romania 2 West University of Timisoara, Timisoara, Romania E-mail address: [email protected] (I.A. Balan). In recent years, food industry introduced in the daily menu many non-natural products to which we cannot resist. Many substances from the agro-alimentary products like additives and preservatives represent a real danger for the human health. A Romanian expression says “Tell me what you eat to tell you who you are!” This saying shows a significant truth that there is a strong relationship between diet and health. An analysis of the food consumption helps us to realize the profile of the consumer in terms of life style, agro-alimentary education and importance paid to the diet and health. An important role in ensuring and maintaining the population health is played by the educational environment which informs the consumers of agro-alimentary products about the benefits of a correct consumption and of bio-ecological origin. We need to ensure the educational framework related to the agro-alimentary area, to train the population in order to help them stimulate the health parameters, to be aware of the benefits on the human health. In the present days many countries focus on the health diet, but this means an active involvement of the decisional factors and a considerable financial effort. In Romania by the involvement of different actors (authorized institutions, NGO’s) there are projects in the educational institutions, campaigns which help educating the children in terms
S91
of healthy diet (“Milk and croissant”, “Fruits in schools”, “Health begins in the plate”). http://dx.doi.org/10.1016/j.jbiotec.2016.05.323 Molecular and ultrastructural aspects in invasive mammary carcinoma Corina Elena Mihalcea 1,∗ , Liliana Puiu 1 , Ana Maria Morosanu 2 , Daniela Murarasu 1 , Silviu Voinea 1 , Sabin Cinca 1 , Nicolae Mirancea 2 1
Institute of Oncology, Department Carcinogenesis and Molecular Biology, Bucharest, Romania 2 Institute of Biology, Romanian Academy, Department Plant and Animal Cytobiology, Bucharest, Romania E-mail address: corina [email protected] (C.E. Mihalcea). Mammary carcinoma is the most non-skin malignancy in women. More than 95% of breast cancers are adenocarcinomas which are divided into in situ- or invasive-carcinoma. Based on similarities in the gene expression profile, breast cancers can be also classified into distinct subgroups, useful to establish appropriate therapeutic protocol. Here we present some peculiar aspects concerning infrastructure of tumor cells involved in invasive process, especially desmosomal junctions, invadopodia and shedding membrane vesicles. Moreover, we report about new described cell phenotype termed telocytes involved in cell signaling by their homo- and hetero-cellular contacts. Telocytes from mammary tumor stroma exhibit a reduced number of hetero-cellular contacts, which suggests a possible perturbation of tissue homeostasis modulation. Data on structural aspects were correlated with studies on PIK3CA mutations – gene involved in proliferation, differentiation, and cell survival, that revealed a high incidence (87.5%) of hot spot mutations, most of them located in the kinase domain; in this regard, there were determined: the frequency of mutations in exons 1, 9 and 20 of the PIK3CA gene, gene copy number variation and the relationship between somatic mutations and gene amplification in breast tumors. Acknowledgment: The work was financed by project Partnership no 4/2012, UEFISCDI, Romania. http://dx.doi.org/10.1016/j.jbiotec.2016.05.324 Obtaining vegetable juices with high nitrite concentration using Staphylococcus xylosus ATCC 29971 strain as source of nitrate reductases Sabina Blascu ∗ , Corina Predescu, Camelia Papuc, Valentin Nicorescu, Constantin Savu University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania E-mail address: blascu [email protected] (S. Blascu). Vegetables naturally accumulate high amounts of nitrate. The amount of nitrate in plants varies among plant species and even among parts of the same plant (roots, leaves). Staphylococcus xylosus is a microorganism able to produce nitrate reductases that convert nitrate to nitrite. The aim of this study was to obtain the highest concentration of nitrites by reduction of nitrates from Iceberg and Romaine lettuce juices in the presence of nitrate reductases produced by S. xylosus ATCC 29971 strain.