Molecular cloning and sequence analysis of the human parainfluenza type III virus

104

MOLECULAR CLONING AND SEQUENCE ANALYSIS OF THE HUMAN PARkINFLUENZA TYPE III VIRUS. MARK S. GALINSKI, MICHAEL A. MINK, DENNIS M. LAMBERT, STEVEN L. WECHSLER, James N. Gamble Institute and MARCEL W. PONS. Auburn Avenue, Cincinnati, Ohio 45219. USA. The human parainfluenza logical

agents

In order

involved

to analyze

of the viral acutely

CV-1 cells,

has been characterized subgenomic

mRNA

ISOLATION

TAMAS

virus of

of

analyzed

the for

sequence

surface

chain

protein

in

library 50s and

protein

structural

(N) and

has

data

on in

with

polypeptides, with

conservation glycoproteins function

the

amino

F a

Leu,

and

F

Phe

as the

is of

composition. of

in

sequence largely

as

the

a

weaker A

and

with

virus

Extensive

N-terminal

of

the

in

membrane

two

families fusion.

53

but 8

region of

showed

N-terminal

paramyxoviruses

distinct

virus

compowas

hydrophobic,

residue segment strictly conserved well

HA

are

among

and

myxoviruses

site

all

Gly-rich

polypeptides.

at'the

kdal

by

acid

Comparison

different

41

yield

amino

Sendai

to

The

high

The

Ala.

Virology1

immunoadsorption

N-terminal

polypeptide

afid

influenza of

pure

or

of Sweden

A-Sepharose.

determined

polypeptides 4-

the

Departments

by

protein

obtained

Met,

10!5

POLYPEPTIDE.

Stockholm,

electrophoresis.

structure

Ile,

01

isolated

to

determined The

Val,

been

was

Fl

NORREiYl,

S-104

bound gel

was

VIRUS PROTEINS

ERLING

protein

residues. of

F,omology

soecialized

for the major viral

MEASLES

Institutet,

fusion

including

evolutionary its

40

1

homologies F

found

to both viral

nucleocapsid

FUSION

antibodies

similarity

noticed,

sequence of

F

THE

JtiRNVALL2and

(F)

published

considerable

studied

HANS

Karolinska

the

residues

previosuly

also

OF

SDS-polyacrylamide

of 24

,

fusion

component

with

of the viral

PARAMYXOVIRUS

monoclonal

pieparative sition

It I

complex F

OTHER

VARSAN

Chemistry

Measles

and expression

transcripts

The recombinant

identity

have been identified

analysis

CHARACTERIZATION

WITH

M.

organization

of the mRNA

to sequence

etio-

in young children.

(P) will be presented.

AND

COMPARISON

and

clones

Sequence

phosphoprotein

library

has been constructed.

in regard

2141

species.

Recombinant protein.

disease

the molecular

a recombinant

Research,

(PF3) is one of the major

with lower respiratory

in more detail

genome,

infected

type III virus

of Medical

of

The cleavage

highlights

a