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Molecular cytogenetics protocols and applications
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Book reviews
The editor, Dr. Yao-Shan Fan, is from the London Health Sciences Center and the University of Western Ontario in London, Ontario. To write this book, he brought together 63 experts who collectively represent some of the most important pioneers in FISH for research and clinical practice. This book contains the written step-by-step procedures for 27 of the most important FISH methods. I know of no better publication that contains so many current FISH procedures. In one section, this book contains procedures to create and label probes for FISH to study both DNA and RNA targets. This section also contains methods for chromosome microdissections, primed in situ labeling, spectral karyotyping, multicolor FISH, comparative genomic hybridization, color banding, multicolor fiber FISH, multi-telomere FISH, fluorescence genotyping for detection of cryptic telomere rearrangements. This book contains another section of methods for FISH to characterize marker chromosomes and to do interphase FISH for prenatal diagnosis of common chromosome anomalies including circulating fetal cells in maternal blood. This
section also deals with FISH for diagnosis of chromosome anomalies in preimplantation genetic studies, detection of microdeletion syndromes, and uniparental disomy. The final section of the book deals with important new applications of FISH in oncology. These applications include interphase FISH studies for chronic myeloid leukemia, chromogenic in situ hybridization, FISH in pathology, FISH for HER-2/neu amplification in breast cancer, comparative genomic hybridization in cancer investigations, simultaneous fluorescence immunophenotyping and FISH on tumor cells, and BAC resources for molecular cytogenetics. Gordon Dewald Division of Laboratory Genetics Mayo Clinic Rochester, 200 First Street SW Rochester, MN 55905, USA Tel.: +1-507-284-7278; fax: +1-507-284-0043 E-mail address: [email protected] (G. Dewald) doi: 10.1016/S0145-2126(02)00345-4
Book reviews
The editor, Dr. Yao-Shan Fan, is from the London Health Sciences Center and the University of Western Ontario in London, Ontario. To write this book, he brought together 63 experts who collectively represent some of the most important pioneers in FISH for research and clinical practice. This book contains the written step-by-step procedures for 27 of the most important FISH methods. I know of no better publication that contains so many current FISH procedures. In one section, this book contains procedures to create and label probes for FISH to study both DNA and RNA targets. This section also contains methods for chromosome microdissections, primed in situ labeling, spectral karyotyping, multicolor FISH, comparative genomic hybridization, color banding, multicolor fiber FISH, multi-telomere FISH, fluorescence genotyping for detection of cryptic telomere rearrangements. This book contains another section of methods for FISH to characterize marker chromosomes and to do interphase FISH for prenatal diagnosis of common chromosome anomalies including circulating fetal cells in maternal blood. This
section also deals with FISH for diagnosis of chromosome anomalies in preimplantation genetic studies, detection of microdeletion syndromes, and uniparental disomy. The final section of the book deals with important new applications of FISH in oncology. These applications include interphase FISH studies for chronic myeloid leukemia, chromogenic in situ hybridization, FISH in pathology, FISH for HER-2/neu amplification in breast cancer, comparative genomic hybridization in cancer investigations, simultaneous fluorescence immunophenotyping and FISH on tumor cells, and BAC resources for molecular cytogenetics. Gordon Dewald Division of Laboratory Genetics Mayo Clinic Rochester, 200 First Street SW Rochester, MN 55905, USA Tel.: +1-507-284-7278; fax: +1-507-284-0043 E-mail address: [email protected] (G. Dewald) doi: 10.1016/S0145-2126(02)00345-4